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THE EFFECT OF ELECTROMAGNETIC AND MAGNETIC FIELDS

ON THE CENTRAL NERVOUS SYSTEM

By Yu. A. Kholodov

Translation of "Vliyaniye elektromagnitnykh i magnitnykh

poley na tsentral'nuyu nervnuyu sistemu."

Academy of Sciences, USSR, Institute of Higher

Nervous Activity and Neurophysiology.

Izdatel'stvo "Nauka." Moscow, 1966.

NATIONAL AERONAUTICS AND SPACE ADMINISTRATION

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TABLE OF CONTENTS

FROM THE AUTHOR 1

INTRODUCTION 4

PART I.. THE ELECTROGRAPHIC METHOD OF STUDYING THE EFFECT OF

ELECTROMAGNETIC FIELDS ON THE CENTRAL NERVOUS SYSTEM 8

EXPERIMENTAL METHODOLOGY AND TREATMENT OF THE RESULTS 9

Methods Used Under Different EMF 9

Methods of Recording the Electrical Activity 11

Surgical Methodology 12

Methods of Treating the Experimental Data 15

CHAPTER 1. THE EFFECT OF A UHF FIELD ON THE ELECTRICAL ACTIVITY

OF THE RABBIT BRAIN 19

The Effect of a UHF Field on the Central Nervous System 19

Changes in the EEG of Rabbits Following the Influence of

a_ UHF Field of Thermal Intensity 22

Changes in the EEG of Rabbits During the Influence of a^

UHF Field of Weakly Thermal Intensity 25

The Effect of a_ UHF Field on the Assimilation Reaction

to a Rhythm of Li;^ht Flashes 31

Physiological Analysis of the Mechanism of Effect of a^ UHF

Field on the Electrical Activity of Rabbit Brain 32

Role of the Distance Receptors in the EEG Reactions

of Rabbits to a_ UHF Field 33

The EEG Reaction to a UHF Field After Removal of the

Cervical Sympathetic Ganglia 37

The EEG Reaction to a UHF Field Following Damage to the Hypo-
thalamus , Thalamus and Reticular Formation of the Midbrain . . 40
The Effect of a UHF Field on the EEG Reaction of an

Isolated Rabbit Brain Preparation 43

The Effect of a UHF Field on the Electrical Activity of a

Neuronally-Isolated Strip of Cerebral Cortex 47

Discussion 56

Conclusions 59

CHAPTER 2. THE EFFECT OF AN SHF FIELD ON THE ELECTRICAL ACTIVITY

OF RABBIT BRAIN 61

The Effect of an SHF Field on the CNS 61

The Effect of a Constant SHF Field of Thermal Intensity

on the Rabbit EEG 65

The Effect of an SHF Field on the Reactance Curve 66

111

The Effect of an SHF Field on the Electrical Activity

of a Neuronally-Isolated Cortical Strip 67

The Effect of a Modulated SHF Field on the Rabjbit EEG 70

The Effect of Caffeine on the EEG Reaction of Rabbits During

the Influence of a Constant SHF Field 71

The Effect of Pulsed SHF Fields of Thermal and Nonthermal

Intensity on the Rabbit EEG 72

The Effect of an SHF Field on the Rabbit EEG After Sectionin&

at the Level of the Midbrain 76

The Dependence of the EEG Reaction of an Intact and an

Isolated Brain on the Intensity of an SHF Field 77

The Effect of Caffeine on the EEG Reaction of an Intact and an

Isolated Rabbit Brain During the Influence of an SHF Field . . 78

Discussion • ^f

Conclusions

CHAPTER 3. THE EFFECT OF A CONSTANT MAGNETIC FIELD ON THE

ELECTRICAL ACTIVITY OF THE RABBIT BRAIN 86

The Biological Effect of Magnetic Fields 87

The Effect of a. Constant Magnetic Field on the Rabbit EEG 94

The Effect of a CMF on the EEG of an Isolated Brain lOO

The Effect of a CMF on the Electrical Activity of a Neuronally-
Isolated Strip of the Cerebral Cortex 103

The Electrical Reaction of Different Sections of the Rabbit

Brain to a_ CMF ^^^

The Effect of a CMF on the Pulsed Electrical Activity of

Cerebral Neurons l '

112

Discussion

Conclusions

CHAPTER 4. THE EFFECT OF AN ELECTROSTATIC FIELD ON THE

RABBIT EEG ^^^

1 1 Q

Con clusions

Synopsis ^^^

The Form of the EEG Reactions 120

The Reaction to Turn-Off 1^^

Reactions at the Moments Electromagnetic Fields are

Turned On and Off 1^3

The Basic Reaction 1^^

The Direct Effect on the Brain 1^'

PART II. THE CONDITIONED REFLEX METHOD OF STUDYING THE EFFE^ OF

ELECTROMAGNETIC FIELDS ON THE CENTRAL NERVOUS SYSTEM 130

EXPERIMENTAL METHODOLOGY AND TREATMENT OF RESULTS 131

Procedure for Development of Conditioned Reflexes 131

Surgical Methodology 1

Methods of Treating the Results 13^

CHAPTER 5. THE DEVELOPMENT OF CONDITIONED REFLEXES TO

ELECTROMAGNETIC FIELDS IN RABBITS, PIGEONS AND FISH 138

The Development of Conditioned Reflexes to a Constant

Magnetic Field in Rabbits 238

The Development of Conditioned Reflexes to a^ Constant

Magnetic Field in Pigeons I39

The Development of a Positive Reflex to a Constant

Magnetic Field 3^39

The Development of Conditioned Inhibition to a

CMF + Light Stimulus 141

The Development of Conditioned Reflexes to Electromagnetic

Fields in Fish -^.^-j

The Development of a Positive Conditioned Reflex 147

Development of Conditioned Inhibition to the

CMF + Light Stimulus I5I

Development of Conditioned Reflexes to a UHF Field

in Fish •|^g2

Development of Conditioned Reflexes to Ionizing

Radiation in Fish 152

Discussion -igg

Conclusions -^M

CHAPTER 6. ANALYSIS OF THE MECHANISM OF THE FORMATION OF CONDITIONED

REFLEXES TO A MAGNETIC FIELD IN FISH I7I

The Effect of Denervation of the Lateral Line Organ on the

Conditioned Magnetic Reflex in Fish I7I

Similarity in the Effects of Light and a Magnetic Field

on Fish 272

Retention of the Conditioned Magnetic Reflex in Fish After

Enucleation I74

The Role of Different Sections of the Fish Brain in the

Realization of a Conditioned Electrodefensive Reflex

to a^ Magnetic Field 178

Development of Conditioned Reflexes After Damage to

the Visual Tegmenta 178

Development of Conditioned Reflexes After Removal of

the Cerebellum I80

Development of Conditioned Reflexes After Removal of

the Forebrain 182

Development of Conditioned Reflexes After Damage to

the Diencephalon 183

Discussion I85

Conclusions I90

Synopsis , I90

PART III . OTHER METHODS FOR STUDYING THE EFFECT OF ELECTRO-
MAGNETIC FIELDS ON THE CENTRAL NERVOUS SYSTEM 193

CHAPTER 7. THE CHANGE IN THE SENSITIVITY OF FISH AND

AMPHIBIANS TO A MAGNETIC FIELD OR LIGHT 194 .

Investigation of Sechenov Inhibition During the

Influence of Light or a Magnetic Field on the

Diencephalon of Frogs with Their Hemispheres

Removed 1^^

The Effect of Light or a_ Magnetic Field on the

Sensit ivity of Fish and Axolotl to an Electric

Current ^^^

Discussion ^^y

Conclusions ^"^

CHAPTER 8. THE CHANGE IN THE MOTOR ACTIVITY OF FISH AND

BIRDS DURING THE INFLUENCE OF A CONSTANT MAGNETIC FIELD 205

The Change in the Motor Activity of Stickleback

During the Influence of a CMF 205

The Change in the Motor Activity of Birds During

the Influence of a OIF 206

D iscussion 211

Conclusions ^^'■

CHAPTER 9. CHANGES IN THE HISTOLOGICAL PICTURE OF THE BRAIN

DURING THE INFLUENCE OF ELECTROMAGNETIC FIELDS 213

Conclusions 216

Synopsis 216

GENERAL CONCLUSIONS 217

REFERENCES 223

THE EFFECT OF ELECTROMAGNETIC AND MAGNETIC FIELDS ON
THE CENTRAL NERVOUS SYSTEM

Yu. A. Kholodov

ABSTRACT. The basic approach of this volume is to clarify
the physiological mechanism of the effect of EMF on the
functions of the brain through the use of the electrophysio-
logical and conditioned-reflex methods. In addition, various
methods of recording motor activity, determining the sensiti-
vity to electrical and chemical stimuli, and certain morpho-
logical methods were used. The experimental objects were
different classes of vertebrates, beginning with fish and
ending with mammals.

FROM THE AUTHOR

The vigorous development of the physical sciences in the XIX and XX centu- A3*
ries is associated with the application of new physical factors in technology,
among which electromagnetic fields occupy an important place. The ^Ventury of
electricity", the "century of radio", the "century of atomic energy", all these
concepts of our epoch involve electromagnetic fields of different frequencies.
There is no doubt that the harnessing of new types of energy will sharply im-
prove human working and living conditions; on the other hand, however, the crea-
tion of an even greater number of power, radio and atomic stations will change,
if we can express it this way, the electromagnetic background of the earth. How
will this affect the health of man, his work capacity and his progeny?

At present, the biological effect of ionizing radiation has been studied to
the extent that its danger has become known to every inhabitant of our planet.
The effect of ionizing radiation now has become not only a biological factor,
but also a political factor in limiting the nuclear arms race. However, prior
to the Second World War not as much was known about the effect of ionizing ra-
diations on an organism. The fact that these radiations did not produce any
sensations in man during their effect made it possible to consider them to be
harmless .

Approximately the same position is now observed with respect to the study
of the biological effect of radio-frequency electromagnetic fields although, of
course, their effect on the organism is manifested to a much weaker degree than
that of ionizing radiation.

It should be emphasized that the problem is not limited to a hygienic eval-
uation of any new physical factor. Electromagnetic fields (EMF) have long been
used for therapeutic and diagnostic purposes. The problem facing the investiga-
tor is not to prove the harmfulness of these factors; they can also be useful.

*Numbers in the margin indicate pagination in the foreign text.

However, to determine their meaning for man we must know the biological mecha-
nisms of the effect of EMF. This knowledge will enable us to further develop
the concepts on the functions of the organism, in particular, the functions of
the central nervous system (CNS) .

It should also be noted that man encounters EMF not only in industry or in
the doctor's office. EMF are an ecological factor to a certain degree. Life
originated and has developed in the earth's EMF, which change periodically and,
possibly, affect certain physiological functions.

Finally, EMF are not only passively absorbed, but they are actively emitted
by certain living structures. Thus, they are a factor of the functional organi-
zation of separate parts of the organism, and, as certain investigators [Granov-
skaya, 1961; Kazhinskiy, 1962; Turlygin, 1942; Presman, 1964a; Mancharskiy, 1964]
assume, they are a factor of communication between organisms.

Among the wide range of EMF we have selected fields of ultrahigh frequency /4
(UHF) with wavelengths from 1 to 100 m, superhigh frequency (SHF) with wave-
lengths from 1 cm to 1 m, and a constant magnetic field (CMF) . The UHF field
attracted us because its biological effect was already well known in the 30 's,
and our problem consisted of conducting an electrophysical analysis of this ef-
fect on the organism. The biological effect of an SHF field is being studied
more intensively at the present. Therefore, we compared our data with the re-
sults of other investigators who use modern physiological methods. The biologi-
cal effect of a CMF cannot now be considered proven. We wanted to validate the
hypothesis concerning the effect of a magnetic field on an organism by methods
that would make it possible to observe the effect of weak EMF. The use of CMF
removed certain methodological difficulties connected with the possible thermal
effect of EMF and the focusing of the electrodes with which electroencephalo-
grams (EEC) were recorded.

Thus, significant sections of the spectrum of electromagnetic oscillations
entered the sphere of our experimental investigations, which, considering the
reference data, allowed us to characterize certain general aspects of the phys-
iological effect of EMF.

In the course of these works we used not only different ranges of EMF, but
also employed different physiological methods and different methods of treating
the data. From the characteristics of just the qualitative properties of the
biological effect of EMF we went to a strict quantitative evaluation of the
changes, using the methods of variational statistics. From this viewpoint, not
all the chapters of this book are equivalent. We wanted to show not only what
was obtained, but also how it was done.

Questions regarding dosimetry, primary mechanisms of the biological effect
of EMF, biochemical changes, etc., remained outside the limits of our investiga-
tion. We conducted only a physiological analysis of the effect of EMF on the
central nervous system of vertebrates. We were interested in the initial physi-
ological changes which occur during the first minutes of exposure, i.e., the
properties of EMF as stimuli. This explains the insufficient development of
many important questions related to the problem of the effect of EMF on the CNS.

The author hopes that this book will promote increased interest in this problem,
which requires further investigation.

I would like to. express my gratitude to my instructors. Corresponding Member
of the USSR Academy of Sciences, Professor M. N. Livanov, and Corresponding Mem-
ber of the RSFSR Academy of Pedagogical Sciences, Professor L. G. Voronin; I
would like to thank Professor M. M. Aleksandrovskaya and Assistant Professor
Z. V. Gordon for their advice and practical aid in this work, and also wish to
express my gratefulness to my colleagues.

INTRODUCTION

Although interest in the biological effect of different physical fields /5
developed long ago, this problem has become particularly urgent in our time.
The question of the biological effect of EMF has been brought up many times, par-
ticularly when physicists discovered a new type of EMF. We shall discuss the
effect of EMF on the CNS in more detail later, but shall now give a brief his-
torical survey.

Even the ancients distinguished magnets from other minerals, not only be-
cause they could attract iron, but also because various healing properties were
ascribed to them. True, each doctor used them in his own manner. Galen used a
magnet as a purgative. Avicenna treated diseases of the liver with a magnet.
Paracelsus used a magnet to treat hernia, dropsy, jaundice and many other dls
eases. Mesmer began by treating certain nervous disorders with a magnet and
ended up acknowledging the existence of an "animal magnetism that affects the
patient just as magnetism affects a mineral. This "animal magnetism , ^o^ever,
was less useful than the "animal electricity" discovered at approximately the
same time by Galvani. A commission of the French Academy of Sciences judged
"animal magnetism" to be unscientific and Mesmer passed into ^Jf f y/^^^f J^^J
charlatan. At the same time, the commission acknowledged the biological effect
of a magnetic field, having written in its report that the healing effect of a
magnet is caused by the direct effect of a magnetic force on the nerves .

The problem of the biological effect of EMF was formulated by V. J. Danilew-
sky (1900), who spoke of the effect of "electricity at a distance . [Transla-
tor's note: i.e., "long-range" effect.] This term was put in quotation marks
by the author himself since, for convenience, all forms of induction were uni-
fied into the concept of "electricity". In the general problem, V. J. Danilew-
sky included "the effect of a magnetic flux, electrical and ^l^^^^^S^^J^^,. ^^
fields, the effect of electrical beams in their different forms and combinations,
including the effect (long-range) of high-voltage and high-frequency currents ,
etc. As objects we must consider not only sectioned nerves and muscles, but
also entire organisms, beginning with the lower organisms, i.e., microbes .
The formulated problem was of great interest for general biology, hygiene ana
electrotherapy .

The first description of the long-range effect of electricity was provided
by Galvani in 1791. He suspended a neuromuscular preparation from a frog on a
wire and placed it in a glass vessel. Upon a spark discharge f^""*/^^/^^^";' ,
cal machine or a "flash of lightning", the muscle contracted. However, although
these experiments were the first of their kind, Galvani's classical investiga-
tions further developed only the electrical contact method of stimulating a
neuromuscular preparation.

Remote electrical stimulation of a frog neuromuscular preparation was also

*V. J. Danilewsky: Issledovaniya nad fiziologicheskim deystviyem elektrl-

chestva na rasstoyanii. (Investigation of the Long-Range Physiological httect
of Electricity.) Volume 1, Kharkov, 1900, pp. 6-7.

produced by Zahn (1868) In the Helmholtz Laboratory by means of the monopolar
ettect of a magneto. The contractions were explained by the effect of an os-
cillatory electrical field. In 1876. Tiegel and Gerens Independently reported
similar tests. Schiff (1879) repeated Charcot's experiments on the restoration
of skin sensitivity in hysterical females by placing their hands in the field of
a solenoid. Magini (1885) noted that the observed effect depends on the orien-
T^^'^oQo"^ ^^^ biological object with respect to the windings of the magneto coil,
in 1888, Hermann published the results of his investigations on the effect of a
magnetic field on a frog neuromuscular preparation. He did not observe muscle
contractions in a magnetic field. The excitability and conductivity of the neu-
romuscular preparation also did not change. We should also note that under
similar experimental conditions McKendrick (1879) observed contraction of a neu-
romuscular preparation when an electromagnet was turned on.

In 1891, D'Arsonval and Tesla independently observed the biological effect
of high-frequency fields on an entire organism for the first time. D'Arsonval
placed his object inside a solenoid that had a high-frequency current flowing
in its windings, and he observed increases in respiration and perspiration,
weight loss, and a reduction in blood pressure in vertebrates. N. Ushinskiy
(1897) noted a weight Increase in guinea pigs under the same conditions.

In 1896, I. R. Tarkhanov first reported on the effect of x-rays on the CNS.

Thus, when V. J. Danilewsky wrote his two-volume monograph (1900-1901), the
literature contained a certain number of disconnected works devoted to the ef-
fect of EMF of different frequencies on a biological object. Although the basic
object of his investigations was a frog neuromuscular preparation, Danilewsky /7
proposed that EMF must cause not only muscle contraction. "We cannot ignore
the other side of the matter, i.e., the possibility of the effect of electro-
kinesis in one form or another on the nutritional processes of nerve fibers and
cells, including all the physical chemistry of this side of life. The nutri-
tional interaction between the axon and the medullated sheath, between the nerve
fiber, the cell and the external plasma of the tissue, the blood, the lymph,
and other physical and chemical conditions of the life of a neuron can no doubt
be modified under the effect of electricity. This in turn can be reflected on
the functions of the nerve apparatus."*

Danilewsky states the purpose of his investigations as the study of elec-
trokinetic stimulation of the frog motor nerve by means of open and closed sec-
ondary magneto circuits.

From this simple problem he went on to study such problems as the electri-
cal properties of the motor nerve, the effects on excitability, sensory nerves,
nerve centers, etc. Then he studied the physiological effect of "electrical
beams ', a magnetic flux, and combinations of various electrical effects.

Electrokinetic stimulation did not differ from contact stimulation except
that the first method did not suppress muscle contractions as the frequency

*V. J. Danilewsky. Issledovaniya nad fiziologicheskim deystviyem elektri-
chestva na rasstoyanii. (Investigation of the Long-Range Physiological Effect
of Electricity.) Volume 2, Kharkov, 1901, p. 103.

of oscillations is increased above 200 Hz, and the reaction was accomplished
with a shorter latent period. Human sensory nerves were also stimulated by EMF.
The sensations were diverse: labored breathing, warmth, tingling, pain. Some-
times when the head was placed in the field, light flashes were sensed. When
the entire subject was placed in the field, animated nervousness sometimes re-
sulted.

After the work of V. J. Danilewsky, it would seem that the problem of the
effect of EMF on an organism would have developed rapidly. However, we observe
the expected interest in this problem only in the 30's; after the extensive in-
troduction of electric lights it became possible to produce powerful UHF
fields. Dozens of monographs and thousands of articles devoted to ^^e biologi-
cal effect of UHF fields were published during these years. The Second World
War abruptly cut off the development of this problem.

The atomic explosions over Hiroshima and Nagasaki gave preeminence to the
problem of the biological effect of ionizing radiations on the organism in gen-
eral and on the nervous system in particular. There are numerous works on this
question, and their number is growing steadily. Several monographs devoted to /»
the effect of ionizing radiations on the nervous system have been published re-
cently [Lebedinskiy, Nakhil'nitskaya, 1960; Livanov, 1962; Mlnayev, 1962; Liv-
shits 19611. These publications contain the general propositions regarding
the mechanisms of the effect of penetrating physical factors on the functions
of the nervous system. Knowledge of these propositions must be considered in a
study of the effect of electromagnetic oscillations of any range on the nervous
system.

The post-war appearance of radar stations has pointed out the problem of
the biological effect of EMF of the SHF range. There are already thousands of
reports on this question. Finally, the conquest of outer space, where signifi-
cant changes in the intensity of magnetic fields are possible, and the magnetic
shielding of a spacecraft from ionizing radiations, have again increased inter-
est in the biological effect of a magnetic field.

Of course, we cannot consider these reasons the only motives for scientific
development of the questions on the biological effect of one or another range
of electromagnetic oscillations. The effect of ionizing radiations on an or-
ganism was studied even before the invention of the atomic bomb, SHF fields were
used in biological experiments before the discovery of radar, and the effect ot
a magnetic field on man was investigated when space flights were only dreams.
However, after considering the mentioned changes in human existence, ttie dis-
cussed problems take on even greater importance.

Thus, the urgency of the problem regarding the effect of EMF on an organism
is prompted by the intensive development of many fields of science and technol-
ogy.

The CNS plays a leading role in the reaction of an organism to various fac-
tors of the environment. The method of conditioned reflexes and the electro-
physiological method have played a significant role in the study of reception
of various stimuli from the environment and from within the organism, itiese

SoUg^af SSt ir^:' """ """ -«»=l-ly i« ^-^ investigation of the

Therefore, the main purpose of this work is to clarify the phvsioloeiral
mechanism of the effect of EMF on the functions of the braL by melns J^'elec-
trophysxologxcal and conditioned-reflex methods. Besides these methods, we have
eStr?Lr.nH \ '^'r'''^^""'"^ """"'^y- determining the sensitivi y to
exnerli^n^^r K T"^ ''^"'"^'' ^"'^ ^^"° '^^^^^^^ morphological methods. The
experimental objects were representatives from various classes of vertebrates
beginning with fish and ending with mammals. rueoraces,

One of the leading ideas used in presenting the material is the concept
concerning the similar effect of SHF. UHF and constant magnetic fields on the
lltin' t "i^t '^^^'^ ^^ ^^ difficult to compare the biological effect of /9

Itll tI^- Jt^''°''^ ^^°^^ intensities, in themselves, are difficult to com-

pare. It IS probable, therefore, that we will be most frequently limited to
describing the initial period of general nonspecific reactions which occur dur-
reLl^^'^^ ^°/f ^°"«/yP^« °f EMF of different intensities; in analyzing the
results, the noted similarity of the biological reactions comes to the fore.
FM^ ^'"k^^'u"^ distinctions in the biophysical effect mechanism of the studied
E^ which are explained by their different degrees of penetration through the
surface tissue, the presence of the thermal effect of variable fields, the pres-
ence of an induced electromotive force when a CMF is varied, etc., were brought
out to a lesser degree in our analysis.

The main questions of this investigation, such as the form of the EEG reac-
tion, the direct effect of EMF on the isolated brain, and the presence of a re-
action when the fields were turned off, were answered in experiments in which
tne effect of each field was investigated separately.

Based on the hypothesis regarding the similar effect of SHF, UHF and con-
stant magnetic fields, in certain cases we applied the results obtained in ex-
periments with one of the fields to other fields. Thus, we clarified the role
ot the distance receptors and the effect of partial sympathectomy only in
experiments with a UHF field, and we studied the dynamics of the pulsed activity
of cortical neurons and the reaction of brain neuromia only under the effect of
3- CMF.

^'^^°"^'^°"*^ ^^^ ^°^^ ^^ ^^^^^ ^° compare the physiological effect of EMF of
the studied ranges not only with one another, but also with the effect of such

/^o^x ^^ light, sound, heat, ionizing radiation, and an electrostatic field
(.ESF) .

PART 1. THE ELECTROGRAPHIC METHOD ilO
OF STUDYING THE EFFECT OF ELECTRO-
MAGNETIC FIELDS ON THE CENTRAL
NERVOUS SYSTEM

Electrographic methods that can record the direct reaction of the CNS have
been insufficiently used in studying the biological effect of EMF. This can be
explained by two reasons.

First, the wave of interest in the biological effect of EMF observed in the
30' s historically belongs to the period in which the electrographic method of
investigating the CNS had just been developed. Second, there were difficulties
in the methodology; these were connected with the possible effect of EMF on the
electrodes used to record the bioelectric reactions.

We know of only six works published prior to 1960 in which the effect of
EMF on the electrical activity of the brain was studied. These were reports on
the effect of a UHF field on the EEC of rabbits [Pardzhanadze, 1954], the effect
of an SHF field on the EEG of rabbits and cats [Bychkov, 1957 and 1959], of mon-
keys [Baldwin et al., 1960] and of man [Sinisi, 1954], and also the effect of
EMF of an undetermined frequency characteristic on the EEG of rabbits [Livanov ^
et al., I960]. In recent years we have noted an increase in this type of inves-
tigation, in which great attention has been devoted to the mechanism of the ef-
fect of EMF on the CNS [Bavro, Kholodov, 1962; Bychkov, 1962; Khvoles et al. ,
1962; Gvozdikova et al., 1964a, b; Vyalov et al. , 1964; Zenina, 1964; Nikonova,
1963; Kholodov, 1962a, b, c; 1963a, b, c; 1964a; Kholodov, Yanson, 1962a, b;
Kholodov, Luk'yanova, 1964; Fleming et al. , 1961; Dinculescu et al. , 1963;
Becker, 1963 and others]. Thus, with each year electrographic methods are ap
plied more widely to investigate the biological effect of EMF, and it is now
time to introduce certain results of these investigations.

We should note that the electrographic analysis of the effect of ionizing ill
radiation on the nervous system is based on the recent monograph of M. N. Livanov
(1962) . Knowledge of the material in this book is necessary to study the effect
of penetrating factors on the CNS, and we will often turn to it in the future.

All our tests were conducted on unanesthetized rabbits.

In studying the effect of different EMF on the electrical activity of the ^
brain, we used the same methodology and methods of treating the data, a descrip
tion of which will be given later.

EXPERIMENTAL METHODOLOGY AND TREATMENT OF THE RESULTS
Methods Used Under Different EMF

In all tests the rabbits were placed on a wooden stand with their spine
upwards: the four extremities and head were secured with bandages.

In most tests we placed the field on the head region. We used total-body
exposure only under a pulsed SHF field. In certain tests we separately focused
the field on the head, chest, intestines, pelvis and hind legs.

Sources of the UHF field (wavelength, 6.6 m) were a UVCh-300 (UHF-300)
generator giving a field strength of about 5,000 v/m (thermal dose) and a UVCh-
2m-40 generator giving a field strength of about 1,000 v/m (oligothermal dose).*
The latter generator was designed for dc feeding, which allowed recording the
electrical activity of the brain during the influence of the field. The plate
electrodes of the UHF generator were placed bitemporally 12 cm apart so that
the entire head of the rabbit was placed between the electrodes (Figure 1) .

A constant SHF field
(wavelength, 12.5 cm) was creat-
ed by a Luch-58 (Beam-58) gen-
erator. The emitter was
placed above the head of the
rabbit at different distances
(Figure 2) . The power flux
densities were 100-200 mw/cm

2
and about 1,000 mw/cm . These

field strengths have a thermal
effect. In some tests the con-
stant SHF field was modulated
by electrical oscillations
with frequencies of 1, 5, 10
and 50 Hz with the aid of a
special attachment to the gen-
erator developed at the VNIIMIiO
(The All-Union Scientific Re-
search Institute of Medical
Instrumentation and Equipment)
and sound generator ZG-10. The

Figure 1. Position of the Electrodes for Re-
cording the Influence of a UHF Field on the
Head of a Rabbit.

modulation index was about 100%.

A pulsed SHF field (wavelength, 52 cm) was used in both thermal (power flu x/14

*The dosimetric investigations of the UHF and SHF fields were conducted with
the help of B. I. Stepanov and Ye. I. Kurakin, colleagues of the Z. V. Gordon
Physical Hygiene Laboratory of the Institute of Labor Hygiene and Occupational
Diseases; the author expresses his sincere gratitude to them.

density of 50 mw/cm ) and „
nonthermal (10 and 2 mw/cm )
intensities for total body
irradiation of the animal.

A constant magnetic
field was created with one
of our two battery-fed dc
electromagnets. Sometimes
a selenium rectifier was
switched in parallel with
the batteries. With a rheo-
stat switched into the cir-
cuit according to the divid-
er principle we could regu-
late the current power and
thereby change the strength
of the CMF.

Figure 2. The Position of the Emitter for an SHF The electromagnets dif-
Field on the Head of a Rabbit. fered in size. The pole

diameter of one was 90 mm,
of the other, 220 mm. The
gap between the poles , where the head of the secured rabbit was placed (Figure
3), could be changed by moving one coil. Most frequently, we used CMF with
strengths of 200, 400, 800 and 1,000 Oe. The field strength was varied by a
"Norma" fluxmeter. When the electromagnet switch was turned on, the time for
reaching limiting strength was 1 sec; when it was turned off, the CMF disappear-
ed in 0.2 sec.

An electrostatic field
(ESF) was created with a
AF-3 generator. The elec-
trodes were 4 x 6 cm copper
plates. For insulation each
electrode was sandwiched be-
tween two 5-mm thick plexi-
glass plates, whose edges
were glued. These electrod-
es were placed bitemporally
around the head of a rabbit
in such a way that the brain
was between them (Figure 4) .
The rabbit's head was reli-
ably secured in a head har-
ness. We used field
strengths of 1.25, 2.50 and
5.00 kv/cm.

The duration of the
Figure 3. Position of the Electromagnet for a Con- influence of each fi«ld was
stant Magnetic Field on the Head of a Rabbit. most frequently limited to 1

Figure 4. Position of the Electrodes for an
Electrostatic Field on the Head of a Rabbit.

or 3 minutes with an interval of 10-20 minutes. In separate experiments with a
CMF the duration of the exposures varied from several seconds to several hours.
Depending on the purpose of the investigation, the duration of the experiment
with each animal varied from several hours to several months.

Methods of Recording the Electrical Activity

Electroencephalograms (EEG) were recorded with the aid of needle electrodes
driven through the bone. The fastening of leads was always monopolar: an in-
ert electrode was fastened to the nasal bones. Before the test, the upper sur-
face of the animal's skull was usually scalped.

Electroencephalograms were recorded from the sensorimotor, parietal and
visual regions of the cerebral cortex with the aid of recording electroencepha-
lographs developed by the VNIIMIiO or the Edisvan (Translator's note: Edison?)
and Alvar Companies, and also with the aid of a UNCh-6 amplifier of the AMN
(Academy of Medical Sciences) Experimental Plant based on a nine-loop oscillo- /15
graph of the Simens Company. On the same instruments we recorded electrocorti-
cograms and the electrical activity of a neuronally-isolated strip of the cere-
bral cortex. In the last case, the recording was performed by the bipolar
method with cotton-wick electrodes. The distance between electrodes was 3-6 mm.

To record the electrical activity of different subcortical formations of the
brain we used nichrome electrodes (diameter, 100 microns), which were insulated
with resin and resistant to biological effects.

According to the Sawyer coordinates [Sawyer et al. , 1954] with the

Meshcherskiy correction [R. M. Meshcherskiy et al., 1960] and using stereotaxic
apparatus, electrodes were imbedded in the sensorimotor and parietal regions of
the cortex, the hypothalamus, hippocampus, thalamus proper, reticular nucleus
of the thalamus and reticular formation of the midbrain. The hypothalamus is
considered to be the formation most sensitive to different ionizing radiations
[Aladzhalova, 1962], including magnetic fields [Kholodov, 1959]. The hippocamp-
us most clearly changed its electrical activity under the influence of ionizing
radiation [Monnier, Krupp, 1962]. After unilateral damage to the reticular
formation of the midbrain the electrical activity of the cortex did not change
on the side of the damage in response to x-rays [Izosimov, 1961a, b; Lebedev,
1963].

The reaction of nonspecific nuclei of the thalamus was investigated be-
cause electrical stimulation of this section of the brain causes spindle-shaped
oscillations on the EEG, i.e., the characteristic form of the EEG reaction to
SHE and UHF fields. Furthermore, electrodes were embedded in specific nuclei
of the thalamus.

To record the pulsed extracellular activity of neurons, we used a micro-
electrode holder such as that designed by A. M. Melekhova (1961). The micro-
electrode was a glass capillary with a tip diameter of 1-2 microns. It was fill-
ed with 2,5 M KCl. The inert electrode was fastened to one ear.

The activity of the neurons was amplified by a UBP-01 amplifier with a
cathode follower. The activity was recorded from the screen of a two-beam
cathode oscillograph operating on film.

To record the neuron activity the rabbits were preliminarily scalped, then
a 2-mm hole was drilled in the skull, the dura mater was opened, and the pia
mater was punctured with a microelectrode. A micromanipulator was fastened to
the skull bone with dental cement. Rotating the screw of the micromanipulator
slowly embedded the microelectrode into the brain. When we observed a spontan-
eously stimulated neuron, we recorded its activity. Recordings were taken be- /16
fore and during the effect of the CMF on the animal's head.

Besides recording such spontaneous electrical activity of the brain, to
elicit the presence of a reaction to the EMF we also used test stimuli methods.
For purposes of testing, we used the reactance curve method (after M. N. Livanov)
and the reaction of the visual cortex neurons to a single light flash. In both
cases the parameters of the electrical reaction to the light stimulus were de-
termined before, during and several minutes after the influence of the field.

In several series of experiments we investigated the electrical reaction of
the brain to EMF after certain pharmacologic preparations had been injected.

Surgical Methodology

To determine the physiological mechanism of EMF perception, we destroyed
the remote receptors and different sections of the brain, performed partial
desympathization, and made an isolated brain preparation (cerveau isol^, in
certain cases encephale isole) and a neuronally-isolated strip of the cereor

:erebral

cortex. The electrical reaction to EMF was investigated both before and after
such surgical intervention.

To exclude vision, we performed a bilateral section of the optic nerves.
In an unanesthetized animal that was secured in a head harness, we cut a section
of the skin under the orbit and cut out a small section of bone immediately in
back of the eyeball with a spatula; trying not to interfere with the blood ves-
sels we moved the muscle tissue in order to observe the optic nerve. It was
sectioned with a scalpel during visual contact. After the nerve was sectioned,
we sutured the skin and performed a similar operation on the other side.

The auditory analysor was destroyed according to the method described by
A. B. Tsypin and Yu. G. Grigor'yev (1961). With a syringe needle bent at a
right angle, we injected 1-3 cc of 96% ethyl alcohol into the middle ear. This
destroyed not only the auditory, but also the vestibular analysor, although in
the future we shall call these animals "deafened".

Macroscopic investigation of the outer, middle, and inner ears of both rab-
bits showed that the region of the middle ear sustained deep destruction on
both sides during the operation. The tympanic membrane was completely absent.
The ossicles of the middle ear (the malleus, incus and stapes) could not be ob-
served.

Very noticeable destruction was observed in the region of the inner ear /17
(the cochlea). With relative retention of the scala tympani and the receptor
formations of the inner ear, we noted mechanical lesions of the auditory nerve
at the base of the cochlea. These lesions varied in severity, but, as a rule,
the auditory nerve was completely destroyed. In the sections of bone adjacent
to the cochlea, we noted sharply expressed hyperemia. Together with bone resi-
due, we observed certain traces of osteogenesis. The test rabbits were unable
to perceive sound with such destruction of their auditory analysor.*

The olfactory analysor was destroyed by vertical section of the olfactory
bulb. We scalped the animal, trepaned a transverse slot over the olfactory
brain and then, through this slot, sectioned the olfactory bulb with a scalpel.

Destruction of the different brain sections was performed with a DT4-3
diathermy apparatus. We secured the rabbit in a stereotaxic apparatus and em-
bedded a live electrode, which consisted of a No. 2 sewing needle insulated wxth
bakelite over its entire length except for the tip. The inert electrode, which
was a lead plate wrapped in moist gauze, was placed on the animal s side. The
place where the inert electrode was applied was preliminarily shorn. A 0.2-amp
current was passed from 20 to 60 seconds. After the tests the animals were kill-
ed and the results of the lesions were checked histologically.

To remove the upper cervical sympathetic ganglia from a rabbit held with
its back downwards on a stand with the aid of a head harness, we sectioned the
larynx under ether. Moving the muscle tissue aside with a spatula, we found a

*The morphological analysis of the auditory analysor structure in the two
deafened rabbits was conducted by Candidate of Biological Science, I. I. Glezer,
to whom the author expresses his gratitude.

sympathetic ganglion and, grasping it with forceps, we cut the nerve fibers
emerging from it. Then, we sutured the skin on the neck and after a day we sub-
jected the animal to experiments on the influence of EMF.

To obtain an isolated brain preparation (cerveau isole) , we used the follow-
ing method for sectioning the midbrain. An unanesthetized rabbit was securely
fastened in a stand with the aid of a head harness. Its skull was scalped and,
parallel to the transverse suture but remaining 3-5 mm in back of it, we made
a slot for the scalpel with the aid of a dental drill and manicure scissors.
Then, we sectioned the midbrain with a scalpel and partially damaged the occipi-
tal region of the cerebral hemispheres located over it. During this operation /18
the spinal arteries were not damaged, so that blood supply was retained to a
significant degree to the isolated brain preparation; the remainder of the ani-
mal served only as a heart-lung system for the preparation. Two out of 10 rab-
bits died immediately after the operation. Just as with normal rabbits, we re-
corded the EEG from the isolated brain preparation with needle electrodes embed-
ded in the bone.

After the tests the animals were killed, and the brain was removed and fix-
ed in formalin. A morphologic analysis showed that sectioning of the midbrain
was most frequently complete, occurring at the level of the lamina quadrigemina .

In determining the size of the neuronally-isolated strip of cerebral cortex,
we considered the fact that the most convenient and bloodless isolation was
possible in the sensorimotor region of the cortex. Proceeding from the di-
mensions of this region, we always isolated a 5 x 15 nmi strip 5-8 mm deep with
the aid of a U-shaped loop of steel wire 0.4 mm in diameter. A wire that served
as the handle was secured to one of the ends of the loop and perpendicular to
its plane.

Isolation was performed in the following manner. The rabbit's head was
secured in the head harness. The upper part of the skull was scalped using no
anesthetic. We then performed trepanation, revealing the interior part of the
hemispheres. With a scalpel we made a transverse incision in the dura mater in
the region of the olfactory brain of one hemisphere, and deepened it to 3-5 mm.
We vertically inserted the forward edge of the loop into this incision and,
gradually bringing it to the horizontal, we moved it all the way to the posterior
section of the brain. We then moved the loop, located under the cortex and
parallel to the surface of the brain, vertically upwards and pressed the dura
mater against the whole perimeter of the loop with a finger. Through the dura
mater we could see that the loop had completely isolated a strip of the cortex
below it. We then performed the same operation in reverse in order to free the
loop. Thus, the loop passed over the same path twice, effectively isolating the
strip. A histologic check showed that isolation was complete (Figure 5).

We should note, as did other authors [Aladzhalova, Koshtoyants, 1960], that
in the initial experiments we operated on and recorded the activity of strips
from animals that had been injected with Diplacini However, it was observed
that injecting this substance reduced the electrical activity of the strip, so we
conducted the remaining series of experiments on unanesthetized animals.

Figure 5. A Transverse Section of the Brain
of a Rabbit. The Arrow Shows the Neuronal-
ly-Isolated Strip of Cortex.

In the initial experiments /19
we also resected the dura mater,
but subsequently we became con-
vinced that retaining the dura
mater did not introduce signifi-
cant changes in the electrical
activity of the strips, while
it also protected the brain sur-
face from drying out and from
reduced blood flow. Further-
more, retention of the dura
mater allowed conducting repeat-
ed experiments with a strip over
several days.

In some experiments we iso-
lated the strip after preliminary
sectioning down to the level of
the midbrain or the spinal cord.

We sectioned the spinal
cord in the following manner. We sheared the fur from the neck, both below (for
subsequent tracheotomy) and above. Under ether, with a scalpel we made a longi-
tudinal section of the skin along the spine immediately behind the occiput. We
separated the muscle tissue with a spatula and removed certain sections with
scissors in order to observe the first cervical vertebrae. Then, the upper part
of the two cervical vertebrae was broken off with forceps, thereby revealing
the spinal cord. The rabbit was injected with Diplacin, tracheotomy was per-
formed and the animal was put on artificial respiration. Electroencephalograms
and the effects of a UHF field in these rabbits were recorded by the same method
as in normal animals. After several sessions, we sectioned the spinal cord
with a scalpel and resumed the treatment with a UHF field on an isolated brain
preparation (encephale Isold) .

Methods of Treating the Experimental Data /20

During each application of any stimulus, there were three portions of the
tracing of electrical brain activity: the background, the period of exposure
and the period of the aftereffect. Frequently, each portion was 1 minute long
and we evaluated the change in electrical activity under the influence of the
stimulus as a result of analyzing a three-minute tracing of the brain electrical
activity. Sometimes the recordings of the background and the period of exposure
were not equal, but the analytical principle remained the same.

Besides the background recording, before each application of the stimulus
we conducted control experiments with a "false" influence; the recording of elec-
trical activity was conditionally divided into background, exposure and after-
effect periods. The control experiments were conducted by exactly the same
method as the tests involving application of stimuli.

A visual analysis of the tracings revealed that changes in the electrical

activity (in comparison with the background recording) did not occur during each
application of the stimulus.

In a number of cases, when a reaction was observed in the EEG of an intact
brain during the period of exposure to different EMF, at the moments the genera-
tor was turned on and off a brief desynchronization of biopotentials occurred.

The characteristic changes in electrical brain activity, which we shall
call the "basic" reaction to the stimulus, appeared several seconds after the
generator was turned on, and frequently continued until it was turned off.
Finally, several seconds after the generator was turned off there were brief
changes in the electrical activity; these we shall call the reaction to switch-
ing off or the "off effect".

Thus, we analyzed 4 types of reactions to the influence of stimuli: 1)
desynchronization at turn-on; 2) the "basic" reaction; 3) desynchronization at
turn-off; 4) the off-effect. Each of these reactions was characterized by a
stability or a recurrence, i.e., the percentage ratio of the number of reactions
to the number of stimulus applications.

The latent period of the desynchronization reactions could not be determined
from the recordings made on the ink-writing electroencephalograph since this
period was a fraction of a second; we evaluated these reactions in terms of
the degree of stability, and sometimes from the duration.

We calculated the average latent period of the basic reaction and the reac-
tion to turn-off, plotted the curve of the distribution of latent periods and 111
determined the stability of the reactions. These data were compared with simi-
lar results from the control experiments.

Sometimes we calculated the average, most characteristic frequency and
amplitude of cerebral cortex biopotentials for each minute of the EEG, taking
the average indices in the background recordings as 100%. The curve of the
dynamics of change in these indices characterized the intensity of the reaction.

Since the basic and turn-off reactions under EMF were most frequently ex-
pressed by an increase in the number of spindle bursts and slow waves, we used
a more detailed method of treatment, calculating the number of these changes
in each 5-second portion of the EEG. Each such portion was characterized by the
alternate presence and absence of these indices. The results of a series of
experiments, which included several tens of exposure periods on several rabbits,
were treated statistically according to the Student criterion for alternative
variability. The following formulas were used:

o = /(iVi + iV.)Pt(l — -P6). (2)

where K^ is the number of spindles or other EEG indices in the background record-
ings;

K is the number of EEG indices during the influence period of the stimu-
lus;

Nj^ is the number of 5-second portions in the background recordings;

N^ is the number of 5-second portions during the influence period of the

stimulus;
Pb is the probability of a spindle being in the background and test re-
cordings;
a is the mean square deviation; and
t is the Student criterion.

When the background recording and the influence period are equal, the
formula is simplified, taking on the form:

t =

(4)

where N is the number of 5-second portions in the background recordings, and
the remaining designations are the same as in formulas 1-3.

In our experiments these results were considered reliable when the Student
criterion was not less than 1.98, which corresponded to a level of significance
of p < 0.05.

The dynamics of the number of spindles and slow waves in the background, /22
during and after the influence period are usually presented in graphic form.

When using the reactance curve method, we calculated the time for the ap-
pearance of the assimilation reaction to light flashes. Using the Student
criterion, we also evaluated the statistical reliability of the difference in
the index in the background and during the influence period of the field, using
the known formula [Rokitskiy, 1961; Beyli, 1964; and others]:

Xj — Xj

V'n\ + '^l' C5)

where x^ is the average time for the appearance of assimilation in the back-
ground;

x« is the average time for the appearance of assimilation during the in-
fluence period;

m^ is the average error for the background; and

in is the average error during the influence period.

During extracellular recording of the electrical activity of separate neu-
rons, the basic index was the frequency of spikes per unit time, which we de-
termined in the background, under the EMF and in the aftereffect period. This
index was determined from the total data for all neurons without exception, and
also from the total data separately for neurons of group I (which increased
their activity during the influence period), group II (which decreased their ac-
tivity during the Influence period) and group III (which did not change their
activity during the influence period) . A neuron was classified in group I or
II only when the frequency of its spikes changed by approximately 2 times during
the influence period.

CHAPTER 1. THE EFFECT OF A UHF FIELD ON THE
ELECTRICAL ACTIVITY OF THE RABBIT BRAIN

The biological effect of a UHF field (wavelength, 1-100 m) was observed by
D Arsonval and Tesla 3 years after this field was discovered by Hertz in 1888.
In the next 70 years the question concerning the effect of a UHF field on a
Vqo^"^?^^''^^" ''^^ investigated in many publications [Libezni, 1936; Shlifake,
1936; Likhterman et al.. 1936; Slavskiy, 1937; Frenkel', 1939-1940; Popov, 1940-
Lxvshxts, 1954; Abrikosov, 1958; and others]. The reader who is interested in
general questions concerning the biological effect of a UHF field will find a /23
sufficiently full description of the ' achievements in this region in these works.
However our purpose is to discuss the effect of a UHF field on the nervous sys-
tem. This subject has been considered in many survey works devoted to the bio-
logical effect of UHF fields, but basically it is discussed in two recent arti-
cles by N. N. Livshits (1957, 1958).

The Effect of a UHF Field on the Central Nervous System

The effect of a UHF field on the functions of the nervous system was noted
even in the first works on the biological effect of such a field. "There is no
doubt that the nervous system, both cerebral and autonomic, is very sensitive
to the influence of UHF waves".*

People subjected to the systematic influence of a UHF field complained of
somnolence, headaches, fatigability and irascibility [Likhterman et al., 1936].
These same authors note that the reactions to a UHF field are distinguished by
their phase nature and reversibility, and they depend on the individual charac-
teristics of the organism and upon the initial functional state. They examined,
both directly and through the reflexes, the UHF field as the unique stimulus
that affects not only the skin receptors, but also the receptor formations lo-
cated more deeply in the organism.

The autonomic nervous system is distinguished by its special sensitivity
to a UHF field. ^

It should be mentioned that many authors distinguish between the thermal
effect of a UHF field, when an increase in body temperature is observed, and a
nonthermal (specific) effect, when no change in temperature is recorded. Under
a strong UHF field the following stages of behavior have been noted in animals:
an increase in motor activity, depression, spasms and death.

Because there is no accurate dosimetry for the UHF field it is difficult
to compare the results of different investigators. However, in most cases non-
thermal or weakly thermal doses were used to study the function of the nervous
system. In this survey we will speak only about the qualitative changes that
occur in the activity of the nervous system under a UHF field.

*y. A. Militsin: Ul ' travysokaya chastota v klinike nervnykh bolezney. (UHF
in Clinical Studies of Nervous Disorders.) Nevropatologiya i Psikhiatriya 7:18
1938.

The effect of a UHF field on the higher centers of the CNS has been studied
experimentally by the method of conditioned reflexes. It has been shown that
a 3-5-minute exposure of a UHF field on the head of a pigeon increases the la- /24
tent period of alimentary reflexes by approximately 2 times. Multiple exposures
lead to a disappearance of positive reflexes, which are slowly restored after
the exposures are stopped [Kharchenko, 1939].

Tests on dogs have shown that the effect of a UHF field on the head in-
creases the conditioned and unconditioned alimentary reflexes. A weakening of
the processes of inhibition has been observed under repeated exposures [Glezer,
1940a]. In normal dogs, in the first phase of the effect, a UHF field in-
creases the alimentary secretory conditioned reflexes, but in the second phase
under repeated application it reduces the positive conditioned reflexes and dis-
inhibits differentiation. Dogs with higher nervous activity disturbances ex-
hibit a deepening of the pathological state during the first phase, and a nor-
malization of activity during the second phase [Promtova, 1956].

During local exposure of the UHF field on the temporal and frontal region
of the cortex of dogs, changes in the conditioned-reflex activity were recorded
in some tests, while in others they were not. The character of the reaction al-
so depended on the type of higher nervous activity of the animal. In dogs with
a strong type of higher nervous activity, the effect of the UHF field on the
auditory region was a reversible lowering of the conditioned reflexes, or disin-
hibition of differentiation only within limits of the auditory analysor. In
dogs with the weak type of higher nervous activity the same exposure reduced
the conditioned reflexes from different analysors [Livshits, 1957].

After multiply exposing rats to an HF field (500 kHz), a shortening of the
latent period of the alimentary motor reflex was noted [Nikonova, 1964].

Thus, an analysis of the experiments conducted according to the conditioned
reflex method shows that a UHF field has a phase- reversible effect on the func-
tions of the cerebral cortex, that this has a cumulative effect, and that it de-
pends on the type of higher nervous activity of the animal and upon the initial
functional state.

After the head of a chicken was placed in a powerful UHF field, the bird
did not drink, eat or even change its position autonomously. An autopsy revealed
hyperemia and reduction of the cerebral hemispheres [Heller, 1932].

Therapeutic doses on the human head produced deflection of extended arms
[Hoff, Weissenberg, 1932] , changes in the auditory thresholds [Sheyvekhman, 1949 J,
a change in the spatial perception of sound [Alekseyenko, 1949], a change in the
flow of sequential visual images [Zagorul'ko, 1948] and an increase in the area
of light sensitivity with a simultaneous reduction in the area of color sensl^
tivity [Bludova et al., 1953]. These reactions did not appear during each ex (l^
posure; this is possibly explained by the direct effect of the UHF field on the
brain, and not on the receptors.

When the head of an animal was placed in a UHF field, a change i^i body tem-
perature [Slavskiy, 1937], an increase in basal metabolism [Tonkikh, iy^ij,

tention of sugar in the blood [Popov and Morkovnikova, 1938] , changes in the
cardiac rhythm [Popov, 1940] and blood pressure [Glezer, 1940b], and also inhi-
bition of the spinal reflexes in frogs [Sych, 1940; Bekauri, 1941] were noted.

Since the effect of a UHF field on the head of a rabbit produced changes in
the activity of the smooth muscles of the intestine, pancreas and salivary
glands, and since a change in respiration was noted in a rabbit under similar
experimental conditions, it was suggested that a UHF field can locally affect
the autonomic centers of the brain [Popov et al. , 1940].

A change in the latent period and the excitability threshold of cerebrospi-
nal reflexes was noted during total-body and local exposure of a UHF field on
the region of the spinal cord [Piontkovskiy, 1936; Kocherga, 1940; Rozanova,
1939; Moskalyuk, 1949; Grishko, 1959]. A low-strength UHF field reduced the la-
tent period of cerebrospinal reflexes in frogs; when the strength was increased
this period lengthened.

Under UHF fields of various intensities, the latent period of the flexor
reflex in rabbits was reduced after 1 minute and increased after 2-15 minutes.
In man, the latent period of the reflex was reduced after 2 minutes and increased
after 20 minutes. This index is considered a sensitive indicator of the effect
of a UHF field [Moskalyuk, 1949].

It is considered that a UHF field affects the central part of the reflex
arc more strongly than the peripheral part, and the afferent more strongly than
the efferent parts. It is possible that a UHF field loosens the protoplasmic
membranes of the intercalary neurons, as a result of which their permeability
increases and the membrane potential decreases [Grishko, 1959].

Morphological investigations of the CNS of animals subjected to the influ-
ence of UHF fields of different intensities frequently reveal hemorrhage
[Slavskiy, Burnaz, 1935; Tolgskaya, Nikonova, 1964], proliferation, and migra-
tion of microglia to the midbrain and the gray matter of the spinal cord [Nikolau
et al., 1934], changes in the neuronal nucleus and in the distribution of Nissl
bodies, tigrolysis in the neurons [Shvarts, 1945], and changes in the axosomatic
synapses of separate nerve cells of the brain, which are manifested as overim-
pregnation and thickening of the end plates and their exfoliation from the
nerve cell body [Tolgskaya, Nikonova, 1964].

Chronic exposure of dogs to a UHF field produced a reduction of the morpho-
logical changes in the CNS in comparison with the data from an acute test
[Zhukhin, 1937], which testifies to the reversible character of the reactions of /26
the CNS to a UHF field [Shvarts, 1945]. During chronic exposure, the greatest
histological changes were observed in the autonomic centers of the hypothalamus
[Shvarts, 1945].

Almost all investigators have noted the inconsistency of the CNS reaction
to a UHF field. This can be explained by the fact that the reaction depends on
the individual characteristics of the human and animal CNS, its initial function-
al state, the phase nature, and localization and intensity [Popov, 1940]. Fur-
thermore, the possible participation of the processes of adaptation or summation
should be considered.

Thus, the participation of the CNS in the reactions of an organism to a UHF
field can be considered proven. Many authors attribute the leading role to the
reflex mechanism, noting that either the reflex is realized directly by a stimu-
lated region on one or another organ, or it is realized secondarily on all sys-
tems of the organism through an essential change in endocrine activity.

The direct effect of the UHF field on different sections of the CNS has been
noted by many investigators, but a comparative evaluation of the role of the re-
flex and the direct effects has been difficult because the reactions have usuaUy
been evaluated from a change in the activity of the peripheral organs and sys-
tems. We know of only one work [Sh. K. Pardzhanadze, 1954, submitted as a the-
sis] devoted to the effect of a UHF field on electrical brain activity. The
author noted an acceleration of biopotentials and an increase in their amplitude
in the cerebral cortex of the rabbit. The respiration rate was also reduced from
72 to 60 cycles a minute; the EKG and the skin temperature did not change.

Simultaneously with our publications on the change in the rabbit EEG under
a UHF field [Kholodov, 1962b, c, 1936b, c, 1964a, b; Kholodov, Yanson, 1962a, b],
reports appeared concerning a change in the rabbit EEG during the influence of a
pulsed low-frequency (2-350 Hz) field [Khvoles et al., 1962] and of a high-fre-
quency (500 kHz) field [Nikonova, 1963, 1964]. Under the influence of low- and
high-frequency fields, the authors noted the appearance of slow, high-amplitude
oscillations in the bioelectric potentials of the cortex.

We have assumed that electrographic methods of investigating the CNS, in
combination with surgery, would allow us to evaluate the importance of direct
reactions of the brain to a UHF field and to qualitatively characterize them.

Changes in the EEG of Rabbits Following the Influence of a 121

UHF Field of Thermal Intensity

We assumed that the higher the UHF field strength, the greater the proba-
bility of a reaction appearing in the EEG. In the first series of experiments,
we recorded the EEG of the visual regions of 4 rabbits for one minute before,
and after 30-60-second exposures to a UHF field with a strength of about 5000
v/m. This duration of the influence period was selected because we noted acute
motor reactions of the animal when it was increased. The interval between ex-
posures was 10-20 minutes because the Initial EEG picture was restored in exact-
ly this time. There were 83 exposures in all. In 18% of the cases we did not
see changes on the EEG, in 32% we observed an increase in the amplitude of the
biopotentials, and in 50% we observed a decrease in the amplitude (Figure 6).
Since we observed an increase in the amplitude of the biopotentials only in one /28
out of four rabbits, we assumed that the most characteristic EEG reaction fol-
lowing the influence of a UHF field of this intensity is a reduction of the bio-
potential amplitude. However, there are still questions concerning the origin
of the changes in the electrical brain activity. Were they caused by a specific
effect of the UHF field on the CNS and other systems of the organism, or were
they caused only by the thermal effect? Are the processes which occur in living
tissues under a UHF field the cause of the changes in the EEG, or are these
changes connected with stimulation (thermal or electrical) of the brain through
the embedded electrodes? An analysis of the reactions is complicated by the

w ■

J t I

-i ! -r- 1 S-

J —4 " . , ,

Figure 6. Changes in the EEG of Rabbit No. 1 (A) and
Rabbit No. 2 (B) Before (I) and After (II) the Influ-
ence of a UHF Field of Thermal Intensity. 1 = Respira-
tion; 2 = EEG of the Visual Cortex; 3 = Time Markings
(1 Sec). Key: (a) 1 Sec; (b) 100 yv.

fact that the rabbits sometimes began to twitch and cry out under the field
which testified to the painful nature of the effect.

After several tests, we noted that ulcers appeared on the forelegs of the
animals. On certain rabbits the ulcers developed to such a degree that the pads
dried out and fell off. Continuing these tests, we noted that the ears of the
animals began to swell. Because of their small dimensions, and because they
were closer to the electrodes, the UHF field could be concentrated on these sec-
tions of the head and legs. To check this assumption we used another method of
harnessing the rabbit on the stand in which the animal's legs were tied to its
back and its ears were held along the midline of the spine. Thus, the ears and
legs of the animal were removed from the surface of the generator electrodes.
The results appeared just as rapidly.

Figure 7 shows a comparative evaluation of the results of EEG tests using
different methods of fixing the animals. Here we have the previously described
results of 83 exposures on 4 rabbits that had their forelegs tied along their
head, and the results of 100 exposures on 15 rabbits that had their forelegs
tied along their back. The duration of the influence period was 30-60 seconds.

Figure 7. Forms
of the EEG Reac-
tion Under UHF
Fields of Weakly
Thermal (A) and
Thermal (B) In-
tensities on the
Rabbit Head. 1 =
Increase in the Am-
plitude of Cortical
Biopotentials; 2 =
Decrease in the Am-
plitude; 3 = Absence
of Changes.

As is evident from the diagram, there was an identi-
cal number of nonreactive cases (18%) with the different
methods of harnessing. When the legs were held along the
head, i.e., next to the electrodes of the generator, the
decrease in biopotential amplitude predominated (50% ver-
sus 32%), but when the forelegs were held along the back,/29
the increase in biopotentials predominated (63% versus
19%) .

It can be assumed that, as for other stimuli [Mnukhina,
1963], phase changes of the EEG occur under a UHF field;
in some rabbits we recorded a desynchronization phase, and
in others, because of their individual characteristics, a
synchronization phase starting at the same time. Postpon-
ing the discussion of the phase characteristics of this
reaction until the end of this section, we now want to in-
troduce certain facts testifying to the reflex character
of the desynchronization reaction. With the forelegs held
along the back, of the 18 cases involving reduced biopo-
tentials following the influence of a UHF field, 12 were
accompanied by a motor reaction. It is probable that in
these cases the UHF field caused a pain reaction, concen-
trating its effect on the ears of the rabbit. If we also
add that after removal of the ears from 3 rabbits, with
their forelegs fixed along their back, we only noted an
increase in the biopotential amplitudes on the EEG after
the effect of the UHF field, then the assumption concerning the painful nature
of its effect becomes more probable in the case of reduced biopotential ampli-
tude. We should also add that exposure of just the hind legs caused only a re-
duction in the biopotential amplitude.

Thus, we can speak of two forms of the EEG reaction following the influence
of a UHF field: an increase and a decrease in the biopotential amplitude (the
first case was most frequently accompanied by a decrease in biopotential fre-
quency, and the second, by an increase; but since there was rarely no change in
frequency, for now, we shall be limited only to an analysis of the biopotential
amplitude) .

Decreases in amplitude (the desynchronization reaction) were observed most
frequently during a painful effect. The animal usually exhibited motor reactions.
The reaction had a sharply expressed reflex character since its source was lo-
calized on the periphery: on the extremities or the ears of the animal, where
the field was concentrated. The cause of this type of EEG reaction probably was
due to heat, as during external heating with the aid of two reflectors we ob-
served a similar reaction. Briefly, the analyzed reaction was a nonspecific EEG
reaction that occurred during the influence of different stimuli and was ably
elicited by painful stimuli.

The second form of the reaction, manifested as an increase of the biopoten-
tial amplitude, occurred during the influence of a UHF field on the head. This
form of the reaction did not change when we removed the electrodes from the skull
during the influence period, then refastened them after this period was over.

Consequently, this type of EEC change was connected with the influence of the
UHF field on the tissue of the head of the rabbit, and not with possible stimu-
lation of the brain through the electrodes.

It was possible to assume that high-frequency heating of the head region
had a different effect on the EEC than the same heating of a different section / 30
of the rabbit body. We noted the solution to this problem by using less intense
UHF fields in which a thermal effect was less probable. We also set ourselves
the problem of studying the rabbit EEG during the influence of the field in or-
der to find out the latent period and the initial character of the bioelectric
reaction. Therefore, in later investigations we used a weaker UHF field.

Changes in the EEG of Rabbits During the Influence of a UHF
Field of Weakly Thermal Intensity

When we began using a weaker UHF generator, holding to the previous metho-
dology, we decided to find out if the less intense UHF field caused heating.

We measured the skin and rectal temperature with a TSM-1 (058) electric
thermometer before and after the influence of the UHF field. It turned out that
a prolonged influence period (several tens of minutes) not only did not increase
the temperature of the skin on the head, but sometimes it even lowered it. For
example, in one rabbit the temperature of the ear before the influence was 35.9°>
after 13 minutes of the influence, 35.6°, after 27 minutes, 34.1°, after 60 min-
utes, 25.2°, after 148 minutes, 25.0°, and after 200 minutes, 25.2''C. Control ex-
periments in which a rabbit was only fastened to the stand and not subjected to
the field showed that just fastening the rabbit caused an increase in its tem-
perature, which then dropped slowly. Similar results are described in the lit- / 31
erature [Kondrat'yeva, 1958]. We later determined the temperature of the ear,
head and rectum for several rabbits during the influence of the UHF field. Ta-
ble 1 gives the results of measuring the skin temperature of the parietal sec-
tion of the head and ear, and also the rectal temperature in 7 rabbits before
and after a 3-minute exposure to a UHF field. The UHF field was not shut off
when the temperature was measured.

TABLE 1. TEMPERATURE OF THE SKIN OF THE HEAD AND EAR AND THE
RECTAL TEMPERATURE OF RABBITS BEFORE AND AFTER A 3-MINUTE EX-
POSURE TO A UHF FIELD WITH A STRENGTH OF 1,000 V/M ON THE HEAD.

o
u

Temperature of the
skin of the head, °C

Temperature of
the ear, "C

Rectal tem-
perature, °C

initial

final

differ-
ence

initial

final

differ-
ence

initial

final

differ-
ence

2
3
4

31.4
31.5
32.2
32.0

30.8
31.8
32.6
31.8

-0.6
+ 0.3
+ 0.4
-0.2

36.9
37.2
28.8
37.4

36.6
36.8
28.6
37.1

-0.3
-0.4
-0.2
-0.3

38.0
38.2
39.8
39.5

38.2
38.1
39.6
39.4

+ 0.2
-0.1
-0.2
-0.1

TABLE 1. (CONTINUED)

•

o
u

Temperature of the
skin of the head, "C

Temperature of
the ear, °C

Rectal tem-
perature, °C

•rt

initial

final

differ-
ence

initial

final

differ-
ence

initial

final

differ-
ence

5
6

31.9
31.8
35.0

31.8
31.6
35.2

-0.1
-0.2
+ 0.2

36.8
35.2
32.8

37.2
34.8
34.0

+ 0.4
-0.4
+ 1.2

39.2
37.2
37.3

39.2
37.3
37.4

+ 0.1
+ 0.1

32.3

32.2

-0.1

35.0

38.5

As the average results of our measurements show, the field did not affect
the temperature (at least in the cases when we measured it).

In contrast to the influence of a UHF field of thermal intensity, during
this series of tests, under the influence of a less intense UHF field, we did
not once observe vocal or motor reaction by the rabbits, salivation did not oc-
cur once, nor did trophic lesions appear. Heartbeat and respiration rate did
not change. The rabbits did not deteriorate, and not one of them died, although
the experiments were conducted for several months (up to six months).

Table 2 gives the qualitative characteristics of the influence of UHF fields
of different intensities and of the thermal effect (bilateral heating with the
aid of two reflectors up to a temperature of 50-60°C) on the head of the animal
according to the reactions recorded in our experiments.

TABLE 2. COMPARATIVE CHARACTERISTICS OF THE INFLUENCE OF
UHF FIELDS WITH STRENGTHS OF 1,000 AND 5,000 V/M AND OF A
THERMAL EFFECT ( 50-60 °C) ON THE HEAD REGION OF A RABBIT.

Reaction

UHF field, v/m

Heating

1,000

5,000

EEG

temperature

motor

vocal

respiratory

cardiac

salivation

trophic lesions

present
absent

present

The table clearly shows that heating and the Influence of a strong UHF field
caused rather similar physiological reactions in rabbits. It is possible that
this similarity is explained by the nonspecific effect of any strong stimulus,
but this assumption does not explain the different character of the EEG change /32
in rabbits under the influence of a heat or a UHF field. The EEG index turned
out to be the most interesting of all the indices of physiological reactions we
recorded.

First, during the influence of a 1,000 v/m UHF field for 3 minutes, of all
the recorded reactions, we only saw changes on the EEG, which testifies to the
greater sensitivity of the bioelectric processes of the brain to this factor.
Second, a common effect of a UHF field of different intensities in our experimen-
tal conditions was the similar character of the changes on the EEG (an increase
in the amplitude). During later investigations, to characterize the EEG reac-
tion to this physical factor more completely, we used only UHF fields with a
strength of 1,000 v/m.

If we disregard the artifact that appeared at the moment of turn-on and the
brief (several seconds) reaction of desynchronization, we did not note any abrupt
changes on the EEG immediately after the generator was turned on.

According to visual evaluation, changes either did not occur on the EEG, or
they appeared several seconds (as a rule, more than 10) after the generator was
turned on and had a very diverse character in both form and duration. To decide
which EEG changes should be considered as reactions to our activities, we con-
ducted a number of control experiments (30 recordings on 7 rabbits) in which the
same procedure of recording the EEG was retained, but the influence of the fxeld
was absent. It turned out that the majority of EEG changes occurring under the
influence of the field could also occur under "false" influences. Although only
a qualitative comparison of the EEG under the real and "false" influences could
not testify to the absence of a reaction, we soon decided to consider only the
changes that were not encountered in either the background recording or the con-
trol experiments as reactions.

Proceeding from this assumption, we called the change in the EEG that was
expressed in the appearance of high-amplitude slow potentials, a bioelectric re-
action; these potentials occurred during UHF treatment, gradually increased in
amplitude and continued for some time after the treatment ceased. The EEG shown
in Figure 8 can serve as an illustration of this definition of the reaction.

It must be acknowledged that our definition of the reaction has a somewhat
formal character, since only the definite qualitative character of the EEG changes
and only prolonged changes are considered. These changes had to last at least
30 seconds and be preserved in the aftereffect. It is reasonable to assume that
the reaction to the UHF field could depend on the initial functional state of /33
the CNS and could be manifested not only as an Increase in the amplitude of cor-
tical biopotentials, but also as a decrease. The duration of the EEG changes
could be less than 30 seconds, could have a phase character, and these changes
may not be preserved in the aftereffect period.

In the most characteristic cases the EEG changes began with the appearance
of single high-amplitude slow waves, which initially alternated with background

. i

(a) 200 MKe\

i cex

(b)

Figure 8. Changes in the EEC of a Rabbit During the
Influence of a UHF Field of Weakly Thermal Intensity.
A Continuous Recording of the EEC of the Visual Cor-
tex. The Arrows Indicate the Moments the Generator
was Turned On and Off, Key: (a) 200 yv; (b) 1 Sec.

activity, but then occupied ever-increasing sections of the EEC and, finally,
began to dominate all other types of activity. After the generator was turned
off, we noted reverse changes in the EEC. Reversibility of the EEC changes fol-
lowing the influence of the UHF field is an essential index of the presence of
a reaction.

In the initial experiments, we recorded the EEC from the sensorimotor, pa-
rietal and occipital regions of both hemispheres. In the sensorimotor region, /34
the reaction was manifested as an increase in the number of spindle bursts.
However, we received the sharpest changes of electrical activity from the occi-
pital region in the form of an increase of the slow waves during the influence.
Therefore, in later experiments we frequently recorded the EEC from only this
section of the cortex. Most frequently, changes in the EEC occurred simulta-
neously in all leads, which indicated the diffuse character of the reactions to
a UHF field.

Being certain of the existence of an EEC reaction to a UHF field, we decided
to characterize it quantitatively. The essential fact is that the reaction does
not appear for each influence period of a UHF field of the same intensity. We
introduced the concept of "reaction stability" and determined it quantitatively

by the percentage ratio of the number of reactions to the number of exposures.

Depending on their individual characteristics, in 34 rabbits the reaction
stability varied from 25 to 76%, averaging 47 ± 2%. The fact that in one rabbit
the reaction stability varied from test to test, indicated its dependence on the
initial functional state.

Thus, the EEG reaction to a UHF field actually exists. But why does it
have such a low stability? We can assiome that this is explained by the weak na-
ture of the stimulus. It is appropriate to recall that under the influence of
a stronger UHF field, the reaction stability attained 82%. However, the suffi- / 35
ciently intense and prolonged EEG reaction occurring in half of the cases testi-
fies that the reaction stability is also determined by the level of excitability
of the structures on which the UHF field acts. In connection with this, it is
interesting to follow the dynamics of the stability of the EEG reaction to a UHF
field in one rabbit, and the average dynamics of the stability for several rab-^
bits.

Table 3 gives the results of 20 influence periods on each of 10 rabbits.
The "+" indicates the presence of the reaction, and the "— ", its absence. One
can see that the test results on rabbit no. 1 demonstrate the summation phenome-
non, i.e., the number of reactions increases as the nximber of exposures increases.
There were 6 reactions for the first 10 exposures, and 8 for the second 10. The
test results on rabbit no. 8 demonstrate the adaptation phenomenon since there
were 6 reactions for the first 10 exposures and only one for the second 10. In
rabbit no. 3, the number of reactions for the first and second 10 exposures was
identical (3).

TABLE 3. STABILITY OF THE EEG REACTION TO
A UHF FIELD IN 10 RABBITS FOR 20 EXPOSURES.

(Ij) HoMep BoaaefiCTBHn

o
(a)!

(c)

S
S
m

2
3
4
5
6
7
8
9
10

+
+
+

+
+

+
+
+

+
+

+
+
+

+
+

+
+
+
+

+
+

+
+
+

+
+

14
7
6
8
5
5
8
7

10
9

Key: (a) Rabbit Number; (b) Exposure Number; (c) Total.

The dynamics of the reaction stability are given on the bottom line. One
can see that the reaction stability varied around an average (40%) and did not
have a significant tendency to increase or decrease. Consequently, the experi-
mentally observed EEG reaction does not obey the laws of adaptation or summation
within the limits of 20 exposures. The appearance of the reaction has a proba-
bility character, and in each specific case it cannot be predicted, but the sta-
bility is characterized with sufficient definitiveness for a large number of ex-
posures .

Going to a quantitative characteristic of the reaction itself, we investi-
gated 66 reactions in 23 rabbits. We visually determined the predominant fre-
quency and amplitude of the cortical biopotentials for a 1-minute interval for
each 3-mlnute influence period (exposure) that caused a reaction. Furthermore,
we determined the same indices for 3 minutes before the generator was turned on /36
and for 1, 3, 5, 7, 10 and 15 minutes after it was turned off. Then we calcu-
lated the average indices for 66 reactions. The dynamics of the changes in the
biopotential amplitude and frequency during the influence of the UHF field are
given in Figure 9. The change in the amplitude is expressed in percent. The
average amplitude of the biopotentials before the generator was turned on was
taken as 100%. On the graph, one can see that in the first minute of the influ-
ence period the amplitude increased by 35%, in the second minute, by 25%, and in
the third minute, by 10%. In 3 minutes, the amplitude increased an average of
70%. Over this same time the biopotential frequency was reduced by a factor of
2. However, we encountered the increase in amplitude more frequently than the
reduction in frequency. When slow waves predominated in the background, the re-
action was manifested only as an increase of the biopotential amplitude. The
correlation coefficient between these indices during exposure was 0.56 ± 0.08.

Thus, the average intensity
of the reaction is manifested as
a 70% increase of the biopoten-
tial amplitude and as a reduction
of the biopotential frequency.
The changes in the EEG caused by
3-minute exposure to a UHF field
are retained after the generator
is turned off. The aftereffect
lasts 10-15 minutes.

Due to the smoothness of the
increase in the biopotential am-
plitude, we did not always manage
to precisely determine the time
of onset of the reaction. How-
ever, a possible error of 1-3 sec /37
in determining the length of
the latent period could not intro-
duce large distortions, since the
spread of the indices was signi-

(a)

' 2 3

t i 3 H b S I

9 10 II tZ 13 m 15

Figure 9. Graph of the Change of the Aver-
age Amplitude (1) and the Average Frequency
(2) of Rabbit Cortical Potentials During Ex-
posure to a UHF Field on the Animal's Head.
I = Background; II - Influence Period (Ex-
posure) ; III = Aftereffect. The Arrows In-
dicate the Beginning and the End of the In-
fluence Period (Exposure). Key: (a) Ampli-
tude, %; (b) Frequency, Hz; (c) Time, Min.

ficant (from 15 to 115 seconds)

Figure 10 shows the distribution curve of the latent periods of 100 reac-

S n 25 35 15 55 55 75 d5 95 IU5 i15 tHi

115

165

Figure 10. Empirical Distribution Curve (1) of
the Latent Period of the Bioelectric Reaction of
the Rabbit Cerebral Cortex During the Influence
of a UHF Field. 2,3 = Theoretical Curves of
Normal Distribution Into Which the Empirical
Curve Can Be Decomposed. Key: (a) = Number of
Cases, %; (b) = Time, Sec.

tions to a UHF field ob-
tained from 37 rabbits. As
can be seen from the figure,
the majority of the reac-
tions have a latent period
of 35 seconds. The curve
has an asymmetric form with
a shift to the right, and
it can be described as the
sum of two theoretical
curves of normal distribu-
tion with the following
parameters: n, = 82, x-, =

42 ± 1.6, o^ = 14.5 and u^
= 18, X2 = 87 ± 4, 02 = 15.

Thus, the EEC reaction
to a UHF field is charac-
terized by a long latent
period. The lengths of
the latent period and the
time of aftereffect allowed
us to determine the method-
ology of future experiments.

Since the reaction occurred maximally in 2 minutes, or did not occur at
all, we established the duration of the exposure at 3 minutes, and considering
the length of the aftereffect, we established the interval between exposures
as 20-30 minutes.

The Effect of a. UHF Field on the Assimilation
Reaction to a Rhythm of Light Flashes

As an index of the functional state of the CNS, we selected reactance curves
of the visual cortex EEC for the influence of light on the eyes in increasing
brightness with a frequency of 3.5 pulses/sec (the reactance curve according to
M. N. Livanov, 1944) . The criterion of cortical excitability was the time for
the appearance of the assimilation to a rhythm of light flashes. We determined
this index before, during and 1 minute after exposure to the UHF field.

From 5 rabbits we recorded 40 reactance curves before, during and after the
exposure.

The average time for the appearance of assimilation before the exposure
was 20.3 ± 0.4 seconds, during the exposure, 17.5 ± 0.3 seconds, and after the
exposure, 18.7 ±0.3 seconds. Statistical treatment showed that the excitabil-
ity of the visual cortex during and 1 minute after a UHF field on the animal's
head reliably increased (p < 0.05).

Thus, the results of tests with the light stimulus supported our conclusions,

reached during an analysis of spontaneous EEG, concerning the change in the func-
tional state of the CNS under the influence of a UHF field on an animal's head.
The application of reactance curves also showed the presence of an aftereffect.
The stability of the reaction to a UHF field determined from the change in the / 38
reactance curve exceeds the stability of the reaction to the same stimulus de-
termined from the change in the spontaneous EEG by almost 2 times. The number
of decreases in the time for the appearance of assimilation to a rhythm of light
flashes during the influence of the UHF field was 88%, and 1 minute after the
generator was turned off, it was 62% (versus 47% of the changes in the sponta-
neous EEG under the influence of the field). Consequently, the spontaneous EEG
recordings do not always indicate the reaction of the CNS to a UHF field, and
the reaction stability is determined to some degree by the peculiarities of the
recorded physiological reaction. To determine the threshold intensity of the
UHF field and to elicit the functional state of the CNS under the influence of
this factor, it is reasonable to apply the more sensitive method of test stimuli.

Because the activity of isolated sections of the brain is difficult to char-
acterize through test stimuli, to clarify the physiological mechanism of the ef-
fect of a UHF field, in later experiments we basically used the method of re-
cording the spontaneous electrical activity of both an intact brain and its sep-
arately isolated sections.

Physiological Analysis of the Mechanism of Effect of a, UHF
Field on the Electrical Activity of Rabbit Brain

After establishing the existence of an EEG reaction to a UHF field, our ba-
sic problem was the clarification of the mechanism of effect of this field on
the organism.

In the first series of experiments, we tried to find out whether all sec-
tions of the rabbit body are sensitive to a 3-minute exposure to a 1,000 v/m UHF
field. For this purpose we placed the animal's hind legs, stomach, chest and
head transversely in the interelectrode space. It is understandable that locali-
zation of the UHF field was relative in these experiments, since the reduced
field exceeded the limits of the interelectrode space. But the test results were
sufficiently definitive: we observed EEG changes only when the influence of the
field was on the head (Figure 11). As was said earlier, the influence of a
strong UHF field on any section of the rabbit body causes a change in the EEG in
the form of a desynchronization reaction. It is possible that a field of this
intensity would cause a change in the EEG if the influence period was increased.
It is also possible that certain physiological reactions not recorded by our
methodology occur in the exposed section of the body during the 3-minute expo-
sure. However, this series of tests definitely showed that the greatest sensi-
tivity to a UHF field is observed in the head region.

The changes that occur in the organism depend not only on the direct effect/40
of an electromagnetic field on the tissues and organs, but they can also be ex-
plained by stimulation of the receptors of different reflexogenic zones, which
has been shown in morphological investigations [Tolgskaya, Gordon, I960].

J-

Role of the Distance Receptors
in the EEG Reactions of Rabbits
to a UHF Field

We decided first to elicit the
role of the known distance receptors
(visual, auditory and olfactory) in
the perception of a UHF field. The

reference data on the effect of EMF on
the functions of these distance ana-
lysers served as the basis for our de-
cision.

Even D'Arsonval (1893) had shown
that accomodation phosphene is sensed
by man during exposure to a variable
magnetic field. This phenomenon was
later supported by many authors

[Danilewsky, 1905; Thompson, 1910;
Magnisson, Steven, 1911; Barlow et al.,
1946; Mogendovich, Skachedub, 1957].
Phosphene sensation occurs most easily
under the influence of a variable mag-
netic field with a frequency from 10
to 30 Hz.

] (a) BOOmhSLj,

Figure 11. Changes in the Rabbit EEG
During the Influence of a UHF Field
of Weakly Thermal Intensity on the
Hind Legs (1), Pelvis (2), Stomach (3)
and Head (4). A = Before the Influ-
ence; B = After the Influence. Key:
(a) 200 uv; (b) 1 Sec.

Changes in dark adaptation of the
retina were noted during the influence
of a UHF field on the human head
[Livshits, 1947]. These changes ap-
peared with a large latent period (3-
4 minutes) and were distinguished by a
long aftereffect (from 15-20 minutes
to 2-3 days) . These peculiarities of
the reaction allowed the author to
state an assumption regarding the for-
mation of the reaction to a UHF field
through the autonomic nervous system
(ANS) . The same assumption was ex-
pressed by L. T. Zagorul'ko (1948),
who had studied the effect of a UHF
field on sequential visual images, K.
Kh. Kekcheyev et al. , (1941) and S. Ya.
Turlygin (1937) , who measured the
thresholds of achromatic vision in man.

G. Demirchoglyan (1953) showed
that the a, b and d waves of the electroretinograms were suppressed and the la-
tent period of the reaction to light was reduced after the influence of a UHF
field on an isolated frog eye. The effect was clearest in experiments with light-
adapted retinas. The author proposed that a UHF field acts on the nerve endings
of the ANS located in the retina or on the molecular structure of rhodopsin.

Thus, by primary or secondary means, a UHF field can affect the activity of
the visual analyser and thereby cause changes in the rabbit EEC.

Our problem also included a determination of the role of the peripheral sec-
tions of the distance analysers, which was accomplished with the aid of denerva-
tion or destruction of the corresponding formations.

We acknowledged that surgery, with which the basic results described in thi s/ 41
chapter were obtained, is an extremely coarse method of influencing the activity
of the whole organism. The reactions of an animal to a UHF field following dif-
ferent types of transections or lesions resemble the reactions of an intact or-
ganism only to a certain degree. Particular care must be used with respect to
negative results obtained after surgery.

But, on the other hand, this method is successfully used by physiologists
in solving various neurophysiological problems, and it allows determination of
the receptor structures and clarification of the role of different sections of
the CNS in various reactions. Therefore, decisive use of the results obtained
in short and prolonged series of tests involving destruction or denervation of
separate structures is a necessary stage in our investigation.

Transection of the optic nerves was previously described. The experiment
involving the influence of a UHF field began either on the day of surgery or the
next day. In all, 3 rabbits were blinded. Two of them were given 20 3-minute
exposures to the UHF field, and the other was given 18. Each day they were
given 2 exposures with 15-20 minute intervals. Immediately after blinding, we
observed changes in the EEC. They were most frequently expressed in a reduction
of the biopotential amplitude. Other investigators [Sarkisov, 1934; Novikova,
1960, 1962; and others] also noted similar changes.

The EEG reaction of blinded rabbits to a UHF field did not differ in form
from the corresponding reactions of intact animals (Figure 12, A). The qualita-
tive characteristics of the reaction to a UHF field by intact rabbits was ob-
tained on the basis of initial experiments on each of the 10 rabbits in which
one of the distance analysers was subsequently destroyed, and from a prolonged
series of tests on 2 normal rabbits. The tests on the intact animals were con-
ducted on the same days as the tests on the subjects.

Thus, the possible effect of individual peculiarities of the animals and the
uncontrollable environmental factors were eliminated to some degree.

The previously described reaction of 34 normal rabbits was characterized by
an average stability of 47% and an average latent period of 48 sec, and the cor-
responding indices for another 12 normal rabbits were 45% and 53 sec. As is evi-
dent, the differences are so insignificant that we can confidently use the data
obtained on the 12 animals for normal characteristics.

In the 3 blinded animals, we observed a reaction stability of 40, 50 and
25%, and an average latent period of 51, 64 and 69 sec, which gave 38% and 61 /42_
sec as averages for the stability and latent period. The reduction in stability
and the increase in latent period of the reaction to a UHF field by the blinded
animals is hardly worth discussing since experiments on 3 animals are clearly

^"■\./*'iv«r»*

2 /V**iv''^''^>'''wW*yVV'^

(a) ,1552w^ [_

(b) '^"^^

Figure 12. EEG of the Visual Cortex of a Rabbit Before
(1) and During (2) the Influence of a UHF Field of Weak-
ly Thermal Intensity Following Destruction of the Visual
(A), Auditory (B) or Olfactory (C) Analysors. Key:
(a) 100 yv; (b) 1 Sec.

insufficient for a quantitative evaluation of the difference in the reactions
between normal and blinded rabbits. However, these experiments allow us to form
a definite conclusion regarding perception of the EEG reaction to a UHF field in
blinded rabbits. Consequently, in the perception of a raF field by an animal,
the retina either does not participate, or its role in the formation of the re-
action is insignificant.

Can the auditory analysor react to a UHF field? According to the data of
N. Yu. Alekseyenko (1949), a 7-mlnute exposure to a UHF field on the head of a / 43
subject destroyed the spatial perception of sound. In similar experimental con-
ditions, B. Ye. Sheyvekhman (1949) observed changes in the threshold of percep-
tion of auditory stimuli. The latest works of Frey (1962, 1963) show that man
can sense, on his head, the influence of EMF with a frequency of 300-3,000 MHz
and an average power flux density of 0.4-7.0 mw/cm^ as sound stimulation.
High-frequency (the 3- and 10-cm range)EMF changed the sensitivity of rats to

sound stimulation [Kitsovskaya, I960]. Investigations were conducted by the
methods of L. V. Krushinskiy (1954) on a genetic strain of rats that react to ^
the sound of an electric bell with a specific motor reaction or convulsive spasms.
EMF (power flux density of 1 or 10 mw/cm2) most frequently reduced the sensiti-
vity of these rats to sound stimulation, which was manifested as an increase in
the latent period of the reaction and a transition of a single-wave reaction in-
to a double-wave reaction. These effects were reversible. Thus, the reference
data allow us to assume the participation of the auditory analysor in the reac-
tion to a UHF field.

Immediately following surgery, the deafened rabbits remained immobile for
several hours. For several days following surgery the EEG was distinguished by
a reduced amplitude and lowered the biopotential frequencies, although the
changes were expressed less sharply than after blinding. Other investigators
[Novikova, 1962; and others] have reported a similar change in the EEG of deaf
ened animals.

The tests involving the influence of a UHF field on deafened rabbits began
approximately a week after surgery and were conducted just as those on the blin-
ded animals. The form of the EEG reaction did not change (Figure 12, B). In
the 3 deafened animals, the reaction stability was 50, 30 and 35%, and the aver
age stability was 45, 68 and 110 sec respectively; the average stability was 38/o
and the average latent period, 74 sec. As is evident, the introduced indices of
the EEG reaction to a UHF field differed little in 2 rabbits from the correspond-
ing reaction of normal rabbits. In the third rabbit we noted a significant in-
crease in the latent period.

Consequently, the EEG reaction to a UHF field can also exist after destruc-
tion of the auditory analysor.

Among the distance analysors, we have yet to analyze the role of the olfac-
tory receptor in the perception of a UHF field. Ye. A. Lobanova and Z. V. Gordon
(1960) observed a reduction in olfactory sensitivity in people who worked under
the influence of SHF electromagnetic fields. Kolin and others (1959) showed
that under the influence of an EMF with a frequency of 1,000 Hz, the subjects i_44
reported a sensation similar to a stuf fed-up nasal cavity.

G. Ya. Khvoles and others (1962) reported that removal of the olfactory
bulbs or painting the nasal mucosa of a rabbit with a 10% cocaine solution stop-
ped the EEG reaction of the animal to a pulsed low-frequency field (frequency,
2-350 Hz; pulsed strength on the order of 100 yv) . Thus, reference data tes-
tify to the participation of the olfactory analysor in the reaction of the CNS
to EMF. Experiments involving the influence of a UHF field began one day after
surgery.

After the olfactory bulbs were removed, changes in the EEG consisting of a
reduction in the potential amplitude were observed [Novikova, 1962]. However,
the form of the EEG reaction to a UHF field remained as before (Figure 12, C) .
For each rabbit, its stability was 30, 15, 20 and 20%, the latent period was 62,
50, 57 and 56 sec; the average figures for the operated rabbits were 21/ and 5/
sec. Thus, after destruction of the analysor the stability of the reaction to
a UHF field was reduced significantly (almost twice), although the average latent

period did not change. Consequently, the EEC reaction to a UHF field can also
exist after destruction of the olfactory analysor.

As a result of a later, larger series of experiments on operated rabbits,
we have become convinced that the EEC reaction to a UHF field is also observed
after simultaneous destruction of the visual and olfactory (2 rabbits), visual
and auditory (1 rabbit), and all three distance analysors (2 rabbits).

The comparative characteristics of the EEC reactions to a UHF field of nor-
mal and operated animals are given in Table 4.

TABLE 4. STABILITY AND LATENT PERIOD OF
THE EEC REACTIONS TO THE INFLUENCE OF A UHF
FIELD IN NORMAL AND DEAFFERENTATED RABBITS.

Test conditions

Number of
rabbits

Number of
exposures

Ntmiber of
reactions

Stability,

Average
latent pe-
riod, sec

normal rabbits

destruction of the
visual analysor

destruction of the
auditory analysor

destruction of the
olfactory analysor

The table shows that destruction of any distance analysor reduces the sta- /45
billty of the reaction to a UHF field and Increases Its latent period to some
degree. However, only the reduction (by 2 times in each rabbit) of the reaction
stability after destruction of the olfactory analysor is significant. The re-
maining operations caused insignificant changes, which sometimes were determined
by the individual characteristics of the animals. It should be noted that sepa-
rate analysors were destroyed by different methods. During destruction of the
visual analysor we sectioned only the optic nerve, during destruction of the
auditory analysor, we mechanically and chemically destroyed the peripheral and
possibly the central part of this and neighboring analysors, and during destruc-
tion of the olfactory analysors, we touched only its central part. Thus, a cer-
tain difference in the results obtained on rabbits after destruction of differ-
ent analysors can be explained by the differences in the methods of destruction.
However, the noted differences do not affect the basic conclusion regarding the
presence of an EEC reaction to a UHF field in rabbits following destruction of
the distance analysors.

The EEC Reaction to a UHF Field After Removal of
the Cervical Sympathetic Ganglia

Many physiotherapists [Shcherbak, 1936; Markelov, 1948] and physiologists
[Orbeli, 1934; Tonkikh, 1940; Livshits, 1958; and others] have noted the impor-
tance of the role of the autonomic (especially the sympathetic) nervous system
in the perception of different physical factors, in particular a UHF field.

Therefore, we set ourselves the task of investigating how the bioelectric
reaction of the rabbit cortex to a UHF field changes following unilateral or bi-
lateral resection of the ganglia cervicale superius.

The literature contains contradictory reports regarding the changes in elec-
trical brain activity following removal of the superior sympathetic ganglia.
Some authors have found no changes in the EEG after unilateral removal of this
ganglion [Shvyrkov and Pukhal ' skaya , 1960], others have found suppression
[Sollertinskaya, 1958, 1960; Karamyan, 1959; Wang T'ai-an, 1960], and still others
have found an increase [Aleksanyan and Arutyunyan, 1959] of slow oscillations of
potential in cortical activity after removal of the sympathetic ganglia. In the
pigeon-rabbit-cat evolutionary class, it is said that the reduction effect in
the amplitude of brain biopotentials following removal of this sympathetic gan-
glion is reduced [Sollertinskaya, 1962].

In our experiments, following unilateral or bilateral resection of the cer-
vical sympathetic ganglia, there was usually a reduction in the biopotential am-/46
plitude. From 4 rabbits, we unilaterally resected the superior sympathetic gan-
glion, but from 2 rabbits, we removed it from both sides. We recorded the EEG
of the occipital regions of both hemispheres before, during and after 3-minute
exposure to a UHF field on the animal's head.

The testing was conducted 1-2 times a day for several weeks. In all, the
6 rabbits were given 64 exposures.

The general results of the experiments are given in Table 5.

TABLE 5. STABILITY AND LATENT PERIOD OF THE EEG REAC-
TION TO A UHF FIELD IN PARTIALLY DESYMPATHIZED RABBITS.

Rabbit
number

Type of sym-
pathectomy

Number of
exposures

Number of
reactions

Stability,
%

Average
latent per-
iod, sec

1
2
3
4
5
6

unilateral
II

bilateral
II

10
5

14
4

4
1

7
8
3
9

40
20
44
57
75
60

33
30
28
35
16
40

total

As the table shows, only the rabbits that were given the least number of
exposures (rabbits number 2 and 5) differed from the average level, which was
characterized by a reaction stability of 49% and an average latent period of 30
sec. These data indicate a certain improvement in the EEG reaction of sympathec-
tomized rabbits to a UHF field in comparison with normal animals, for which the
average stability was 45%, and the average latent period, 53 sec (see Table 4).
In contrast to normal rabbits, in operated animals the EEG reaction was not al-
ways manifested as an increase in the amplitude and a decrease in the frequency
of biopotentials, although this form of the reaction predominated. Sometimes
during the influence, we noted a prolonged decrease in biopotential amplitude
(Figure 13). This tjrpe of change in the potentials occurred only during the in-
fluence and with the same latent period as the increase in amplitude; and it con-
tinued for some time in the aftereffect period. These circumstances allowed us
to consider these changes on the EEG of sympathectomized rabbits as a reaction
to the UHF field.

2 ■:. ^^•^-'^''-^/^•^^^vv'..^^^■JV-AAv,./C.>-v''.^~/->- .-..-^''.,--.v--^-,v

(a) iOOMH8\_^
(b) ^ceK

Figure 13. EEG of the Sensorimotor (1) and Visual (2)
Cortex of a Rabbit Before, (A) and During (B) the In-
fluence of a UHF Field of Weakly Thermal Intensity Af-
ter Partial Sympathectomy. Key: (a) 100 yv; (b) 1
Sec.

It should be noted that a certain aggravation of the reaction in sympathec -/47
tomized rabbits was manifested not only as changes in the form of the EEG re-
action, reduction of its average latent period, and an increase in stability,
but also as an increase of the aftereffect of up to 30 minutes (normal, 10-15
minutes). Based on the data of Table 5, we can assume that after bilateral sym-
pathectomy, the stability of the reaction to a UHF field becomes greater than
after unilateral sjnnpathectomy. However, the small volume of experimental data
does not allow us to insist upon this conclusion at this time.

Thus, the EEG reaction of rabbits to a UHF field is maintained following
resection of the superior cervical sympathetic ganglia.

On rabbits .and cats, K. P. Golysheva (1942) showed that removal of the cer-
vical sympathetic ganglia lessens the rise in rectal temperature during the in-

fluence of a UHF field on the head. In tests on cats, A. V. Tonkikh (1941) not-
ed that after bilateral removal of the stellate and inferior cervical sympathet-
ic eanelia an Increasp. in the basal metabolism V7as not observed in a UHF field.
After removal of the cervical sympathetic ganglia from cats, D. Ya. Glezer
{1940b) noted that the quickening of the cardiac rhythm lagged behind the rise
in temperature under a UHF field, while in control animals a correspondence of
the chronotropic and temperature effects was noted. After sectioning of the
sympathetic pathways from frog extremities, in contrast to local exposure, to-
tal body exposure to a UHF field did not cause contraction of the vessels. N.
Koiwa (1939) showed that after sectioning the sympathetic nerves, the effect of
a reduction in diuresis during the influence of a UHF field was weakened. I. V.
Bekauri (1941) observed that the Secheno^ inhibition in frogs caused by a UHF /48
field was reduced by sectioning the sympathetic nerves.

As this brief listing of reference data reveals, these authors usually re-
corded some peripheral effect caused by a UHF field. Numerous results agree
that the sympathetic nerves frequently serve as effectors for the realization
of the effect of a UHF field on the periphery. However, in our experiments with
recording of the EEG, the sympathetic nerves could fulfill only an afferent
function. As the results show, the sympathetic nerves did not maintain this af-
ferent function and after removal of the superior cervical sympathetic ganglia
the sensitivity of the animal to a UHF field increased somewhat. In the pre-
ceeding cases, when we performed certain surgery (destruction of the distance
analysers), the reaction to the UHF field deteriorated. It is possible that
removal of the sympathetic ganglia increases the sensitivity of the CNS to a
UHF field, primarily the sensitivity of the hypothalamic region, where the
higher autonomic centers are located. Therefore, in the following series of ex-
periments, we decided to investigate how the EEG reaction of rabbits to a UHF
field changes following damage to the hypothalamic region.

The EEG Reaction to a UHF Field Following Damage to the Hypo-
thalamus , Thalamus and Reticular Formation of the Midbrain

These tests were conducted jointly with Z. A. Yanson. In 8 rabbits, we
unilaterally destroyed the hypothalamus and, as a control, in 5 rabbits we de-
stroyed the thalamus, and in 4, the reticular formation of the midbrain. The
tests were begun either on the day of the operation or on the day after. A 3-
minute exposure to a 1,000 v/m field was conducted 1-2 times a day. The chang- /4?
es in the EEG after destruction of any section of the brain were manifested as
the appearance of slow high-amplitude oscillations Immediately following the
operation. This type of change was revealed particularly sharply on the side of
the destruction. The asymmetry of the EEG disappeared 3-7 days after the opera-
tion and we recorded electrical brain activity that differed little from the
corresponding activity of normal rabbits.

The general results of the tests on the influence of a UHF field on opera-
ted rabbits are given in Table 6.

If we recall that in normal rabbits the stability of the reaction to a UHF
field was 47 ±2%, then it becomes clear that after destruction of these sections
of the brain the reaction stability increases, and this increase was particularly

TABLE 6. STABILITY OF THE EEG REACTION TO A UHF FIELD AF-
TER DESTRUCTION OF DIFFERENT SECTIONS OF THE RABBIT BRAIN.

Characteristics
of the operation

Nximber of
rabbits

Number of
exposures

Number of
reactions

Stability
%

destruction of the hypothalamus

destruction of the midbrain

destruction of the reticular
formation of the midbrain

12 3 5

dpeMU ^ nun.

Figure 14. Dynamics of the Change in the
Average Amplitude of Cerebral Cortex Bio-
potentials During the Influence of a UHF
Field in Normal Rabbits (1), After Unilat-
eral Destruction of the Hypothalamus (2),
After Destruction of the Nonspecific Nu-
clei of the Thalamus (3) and After De-
struction of the Reticular Formation of
the Midbrain (4). A = Background; B =
Influence Period; C = Period After the
Influence. Key: (a) Biopotential Ampli-
tudes, %; (b) Time, Min.

significant after destruction
of the hypothalamus. If, how-
ever, we characterize the ob-
tained results by the inten-
sity of the reaction (Figure
14) , then it is evident that
after destruction of the hypo-
thalamus the intensity of the
reaction increased significant-
ly in comparison with the nor-
mal (data obtained from the
same 17 rabbits before the
operation) . After damage to
the nonspecific nuclei of the
thalamus, the intensity of the
reaction of operated rabbits
to a UHF field differed little
from the reaction of normal /50
rabbits, and after destruction
of the reticular formation of
the midbrain, the difference
from the norm towards a reduc-
tion in the intensity of the
reaction appeared essentially
during the aftereffect period.

Thus, an analysis of the
stability and intensity of the
EEG reaction to a UHF field
consistently testifies to an
enhancement of this reaction
after unilateral damage to the
hypothalamus .

The average latent period of the reactions after damage to the hypothalamus
turned out to be the same as in normal rabbits (56 versus 53 sec) .

In form, the reactions to a UHF field in rabbits after destruction of the
hypothalamus were most frequently the same as the reactions of normal rabbits,
i.e., the amplitude of the biopotentials increased and the frequency decreased
or the number of spindles increased. However, sometimes in the operated rabbits,
oscillations appeared in the cardiac rhythm during the influence (Figure 15) ,
which we never recorded in our other animals. Just as the increase in the sta-
bility and intensity of the reaction, the appearance of "autonomic" rhythms tes-
tifies to an increase in the reactance of rabbits to a UHF field following de-
struction of the hypothalamus.

cii

(a) fOOM^S 1 , ■'

(b) fc&< ;

Figure 15. EEG of a Rabbit Before (A) and During (B) the
Influence of a UHF Field of Weakly Thermal Intensity After
Unilateral Destruction of the Hypothalamus. The Numbers
Designate the EEG Leads (See the Diagram). The Bottom Two
Recordings Are an EKG and a Pneumogram. Key: (a) 100 yv;
(b) 1 Sec.

Consequently, damage to the central (unilateral destruction of the hypo-
thalamus) or peripheral (removal of the cervical sympathetic ganglia) sections

of the autonomic nervous system Increases the reactance of rabbits to a UHF
field. It should be noted that the Increase in the reaction is observed mainly
during the first several days after the operation.

The experiments that served as a control to the destruction of the hypothal-
amus showed that damage to the thalamus or the reticular formation of the mid-
brain weakly affects the EEG reaction during the influence of a UHF field.

Graduate Student R. A. Chizhenkova conducted special investigations to clar-
ify the role of the reticular formation of the midbrain, the thalamus and the
hypothalamus in the EEG reaction of rabbits to UHF, SHF and constant magnetic
fields. Her results show that the integrity of these sections of the brain is
not a necessary condition for the existence of an EEG reaction to these physical
factors.

On the other hand, G. V. Izosimov (1961a) observed that exclusion of the re-
ticular formation of the midbrain involves a disappearance of the changes in bio-
electric activity during the early periods after total-body irradiation of rab-
bits with Co , 700 R, using an EGO-2 stand; destruction of the hypothalamus
aggravates the electrical reaction of the brain to irradiation.

Similar results were obtained by I. N. Kondrat'yev (1962) and A. N. Lebedev
(1963). Thus, besides the specific systems, the nonspecific systems of the hypo-
thalamus and the reticular formation of the midbrain also participate in the for-
mation of the early radiation reaction of the cerebral cortex. M. N. Livanov /51
(1962) noted that during exposure to ionizing radiation, afferent impulses also
increased significantly, which affects the initial disturbance of cortical elec-
trical activity following exposure. Destruction of the reticular formation of
the brain stem somewhat reduced the flow of pathological afferent impulsation.
This circumstance also led to changes in the cortical biocurrents appearing to
a lesser degree in the first hours after the irradiation.

Since the EEG reaction of rabbits during exposure to EMF did not change /52
significantly after destruction of the nonspecific formations of the midbrain,
thalamus and hypothalamus, it was assumed that this reaction does not depend on
afferent impulsation and is realized as a result of the direct effect of the
field on the brain. However, peripheral impulses can also reach the cortex
along specific pathways. Therefore, to check this assumption we conducted ex-
periments on isolated rabbit brain preparations.

Starting from the first tests of Bremer (1936), physiologists have widely
used the classic isolated brain preparations, encephale isole and cerveau isole,
to clarify the role of afferentation in the formation of electrical brain activ-
ity, to study the functions of separate sections of the brain, and to derive the
mechanism of the effect of different chemical substances on the CNS. However,
we have not encountered works which used these preparations to clarify the mech-
anism of the effect of physical agents on the CNS.

The Effect of a. UHF Field on the EEG Reaction of an
Isolated Rabbit Brain Preparation

This series of tests was conducted under acute experimental conditions.
These conditions required a new methodological approach. We have already con-
sidered different surgical methods from the standpoint of the activity of the
total organism. Since we could not preserve the life of the animals for more
than one day after the sectioning, it became necessary to apply the influence
of the UHF field more frequently. While previously we were limited to 1-2 ex-
posures per day, fearing that an increase in the number of exposures would some- /53
how change the functional state of the CNS, now the number of exposures per test
was from 2 to 20. Did this intensification of the experiment affect the obtain-
ed results? It turned out that it did not. Some 15-20 minutes after a 3-minute
exposure to a 1,000 v/m UHF field on the head of a rabbit, the effect of the
preceding reaction was not experienced. In this period of time, repair (adap-
tational) processes probably fully eliminate the consequences of such a brief
stimulus.

The fact that we recorded the EEG of the parietal region from the isolated
brain preparation, and the occipital region from normal rabbits, did not have
decisive importance since the EEG reaction to the UHF field had a diffuse char-
acter. Finally, additional damage to the occipital lobes of the hemispheres
during sectioning of the midbrain did not have a noticeable effect on the EEG
reaction of the brain. After sectioning, the experiments lasted 1-6 hours. The
method of exposure to the UHF field was the same as in the experiments with nor-
mal rabbits.

The EEG changed abruptly immediately after sectioning. Slow, high-amplitude
oscillations appeared in the biopotentials in all cases. Spindles sometimes
arose in the background of separate oscillations. However, in spite of the fact
that the sectioning itself caused the same kind of change in the EEG as the in-
fluence of the UHF field, the reaction of the isolated brain preparation (like
the reaction of an intact brain) was manifested as an increase in the amplitude
and a decrease in the frequency of the biopotentials (Figure 16) .

(a) 200mhB\_^
(b) Icex

Figure 16. EEG from an Isolated Brain Preparation Be-
fore (A) and During (B) the Influence of a UHF Field.
Key: (a) 200 yv; (b) 1 Sec.

Table 7 shows the results of the influence of a UHF field on isolated brain
preparations from 10 rabbits.

TABLE 7. STABILITY AND LATENT PERIOD OF THE REACTIONS
TO A UHF FIELD IN AN ISOLATED BRAIN PREPARATION.

Rabbit

Number of

Stability,

Average la-

number

exposures

reactions

tent per-
iod, sec

100

total

33 .

The table shows that we observed a reaction to each application of the UHF /54
field in half of the animals after sectioning the midbrain. The lowest stabil-
ity was 50%. In other words, an analysis of the reaction stability in each
rabbit shows that the isolated brain preparation reacted more frequently to a
UHF field than a normal brain.

Contradistinct to the tests involving destruction of the hypothalamus, in
which the reaction stability also increased significantly, with the Isolated
brain preparation the reaction was observed not only more frequently, but also
with a smaller latent period. This fact is demonstrated by both the averages
of the latent periods (33 sec in the isolated brain preparation in comparison
with 53 sec in normal animals) and the graph of the distribution of the latent
periods of the reactions in normal rabbits and in rabbits after sectioning of
the midbrain (see Figure 20). Most of the reactions after sectioning of the
midbrain took place after a latent period of 25 sec, while in normal rabbits
the mode of the latent period distribution curve occurs at 35 sec. The graph
also indicates that the spread of the results is reduced after sectioning. In
short, after sectioning of the midbrain, the EEG reactions to a UHF field were
more frequent and had a shorter latent period than in normal animals. The ob-
tained results were unexpected. We had assumed that such a severe interference
as sectioning at the level of the midbrain must sharply reduce the reaction to
a UHF field, which is a weak stimulus. However, the operation reinforced this
reaction.

Structures of the dlencephalon and telencephalon that were neuronally con-
nected with only the optic and olfactory nerves were included in the isolated
brain preparation.

When we additionally sectioned the optic nerves on 2 sides in 4 rabbits.

the reaction of the isolated brain to a UHF field did not change either in the
degree of stability, or in the length of the latent period. Consequently, the
structures of the diencephalon and telencephalon, which had retained nervous
connection with only the olfactory analyser, reacted just as after sectioning
of the midbrain.

In other words, if we exclude the humoral mechanism, the structures that
perceive the influence of a UHF field are in the brain itself or in the olfac-
tory analysor system. In discussing this material now, as well as later on, we
have not considered the possibility of the effect of EMF on the sections of the
CNS located below the level of the sectioning.

But how will an isolated brain react to a UHF field after damage to the ol-
factory analysor? To answer this question we conducted a series of experiments
on 8 rabbits which involved the influence of a UHF field on the head after sec-
tioning of the midbrain and additional sectioning of the olfactory brain and
the optic nerves.

The changes in the EEC under the influence of a UHF field on totally deaf-/ 55
ferentated structures of the diencephalon and the forebrain were the same as
during this influence on the cerveau isole preparations. The test results are
given in Table 8.

TABLE 8. STABILITY AND LATENT PERIOD OF THE REACTIONS TO A UHF
FIELD IN A TOTALLY DEAFFERENTATED ISOLATED BRAIN PREPARATION.

Rabbit

Number of

Stability,

Average la-

exposures

reactions

tent per-

number

iod, sec

total

The table shows that half of the animals (nos. 1, 2, 5, 7) reacted to the
UHF field only once for 4-6 exposures. The most sensitive rabbit (no. 3) did
not change its sensitivity to the UHF field after additional sectioning of the
olfactory brain and the optic nerves. However, on the average, the reaction
stability after additional sectioning was reduced by a factor of two, although
the latent period did not change.

If we compare the results given in Table 8 with the results of Tables 4
and 7, we can conclude that in some animals the integrity of the olfactory brain
plays an important role in the reaction to the UHF field (the stability is re-
duced down to 20% regardless of whether sectioning of the olfactory brain was
conducted in an intact or an isolated brain) , and in other animals the reaction
occurs very well even after sectioning of the olfactory brain.

In any case, on the average, the stability of the reaction to the UHF field
of totally deafferentated structures of the diencephalon and telencephalon is
almost equal to the stability of the corresponding reaction of an intact brain
(46 versus 47%), but the latent period of the reaction of the deafferentated
brain is shorter (34 versus 53 sec) .

Thus, we can conclude that the fully deafferentated structures of the dien-/56
cephalon and the telencephalon react better to a UHF field than an intact brain.
Consequently, a UHF field has a direct effect on these structures. The increase
in stability and the decrease in latent period of the reaction to the UHF field
after sectioning of the midbrain can be explained as the removal of the inhibit-
ing effect of afferent impulsation and by an increase in the excitability of
the structures of the diencephalon and telencephalon. Both mechanisms seem
probable, but can just the cerebral cortex react to a UHF field? To answer this
question we conducted a series of experiments, recording the electrical activity
of a neuronally-isolated strip of the cortex.

The Effect of a UHF Field on the Electrical Activity of a
Neuronally-isolated Strip of Cerebral Cortex

In recent years, the method involving a neuronally-isolated strip of the
cerebral cortex has become widespread for answering questions concerning the
origin of spontaneous cortical activity [Kristiansen, Courtois, 1949* Burns
1951; Ingvar, 1955; and others] and concerning the direct effect of certain*
chemical [Preston, 1955; Rech, Domino, 1960; Haiti, Domino, 1961; and others]
or physical factors [Aladzhalova, 1962; Gidlof, Soderberg, 1964] on the elec-
trical activity of the cortex.

We began recording electrocorticograms 10-20 minutes after the operation
and continued for 4-6 hours on the day of the operation, and in some cases, on
the day after. Immediately following the operation, we usually recorded inde-
terminate low-amplitude electrical activity. In comparison with the activity
of the surrounding cerebral sections, the strip was "silent." However, 10-100
minutes after isolation, we began to record high-amplitude potentials from the
strip. We recorded this electrical activity in 61 rabbits. The distribution
of the time of appearance of high-amplitude activity from the strip after iso-
lation is given in Figure 17. We should note that later on, we usually observed
the appearance of electrical activity in the strip (after 1-1.5 hours) after
injection of Diplacin or after sectioning at the level of the midbrain. In a
strip isolated in the parietal-occipital region of the cortex, the activity
usually appeared later than in a strip isolated in the sensorimotor region.

Thus, in an unanesthetized animal, high-amplitude activity occurred in the
strip 30-40 minutes, on the average, after isolation (the mode was 25 minutes).

J « 2i Ji ¥i SS S5 n Si Si Wi

Figure 17. Distribution
Curve for the Time of Ap-
pearance of Electrical Ac-
tivity from a Neuronal ly-
Isolated Strip of the Cor-
tex in 61 Rabbits. Key:
(a) Number of Cases; (b)
Time, Min.

The obtained results contradict the conclu- /57
sions of Burns (1958) concerning the absence of
spontaneous electrical activity in a neuronally-
isolated strip of cat cerebral cortex. According
to his data, certain bursts of spontaneous activ-
ity can be observed for an hour or two after the
operation, but they usually disappear if the strip
is left in a state of total rest. In certain prep-
arations, comprising 10% of the cases, these
spontaneous bursts did not cease, but Burns con-
sidered them to be random and excluded them from
further analysis.

In our experiments, more than 50% of the
preparations retained their activity for 2 hours
after isolation even if no influence was exerted
on the brain. Appearing approximately 1 hour af-
ter the operation, the high-amplitude oscillations
of potential were repeated only rarely at first
(approximately every 20 sec), but then they gradu-
ally began to appear more frequently (every 2
sec) .

As an example, we offer the dynamics of the increase in the frequency of
single high-amplitude oscillations in an isolated strip of the sensorimotor re-
gion of an unanesthetized rabbit (Figure 18). The activity of the strip was
recorded every 5. minutes for 2 hours and 10 minutes. One can see that the ac-
tivity, which appeared 25 minutes after isolation, had reached 12 waves/min at
40 minutes, 24 waves/min at 85 minutes, and 36 waves/min at 2 hours.

Thus, the electrical activity of the
cortical section, destroyed as a result of
isolation, is initially depressed, but then
it gradually increases, taking on a form
that differs from the form of the electri-
cal activity of neighboring cortical sec-
tions .

Some 3-6 hours after the operation,
the electrical activity of the strip either
is depressed again, or (more frequently)
becomes similar to the electrical activity
of the intact cortical sections. On the
day after the operation, the electrical ac-
tivity of the strip differs little from the
electrocorticograms of adjacent sections.
However, during an influence from any stim-
uli on the CNS, the electrocorticograms of
the strip change differently than the elec-
trocortiograms of the intact cortex.

W 211 P W 5/7 SO 70 BO SO HIO HO 120 W

Figure 18. Dynamics of the Fre-
quency of Single High-Amplitude
Oscillations of Biopotentials in
a Neuronally- Isolated Strip of
Cerebral Cortex of an Unanesthe-
tized Rabbit. Key: (a) Number
of Oscillations Per Minute; (b)
Time, Min.

In the literature, we encountered

several works that investigated the electrical activity of an isolated strip of
rabbit cortex [Aladzhalova and Koshtoyants, 1960; Aladzhalova, 1962; Monakhov /58
1963; Repin, 1963]. N. A. Aladzhalova (1962) isolated 6 mm x 4 mm strips, alon^
the surface and 3 mm deep, in the parietal region of the cortex. The rabbit
was given a 0.5 g/kg dose of urethan intraperitoneally or it was immobilized
with Diplacin. Spontaneous electrical activity in the strip was observed in 33
of 52 cases (65%) during periods up to 50 min after the isolation. In our ex-
perimental conditions, spontaneous activity of the strip was observed in 61 of
67 cases (91%), and they lasted for hours, frequently not stopping. The dynam-
ics of the appearance of "spontaneous" activity of the infrequent spindle type
coincides in principle with the dynamics of the corresponding activity we des-
cribed above. N. A. Aladzhalova (1962) gives an example in which the electrical
activity of the strip was reduced sharply 5 minutes after isolation* spindles of
high-amplitude oscillations that were initially repeated with an interval of 80
sec occurred 17 min after isolation; these spindles occurred with an interval of
7 sec by the 25th minute after isolation. The spindles disappeared from the
45th minute on after isolation.

K. K. Monakhov (1963) recorded the electrical activity of an isolated strip
under conditions of drugged sleep (sodium amytal, 0.1 mg/kg) . High-amplitude
discharges were observed in the strip after it was electrically stimulated.
I. S. Repin (1963) reported that the spontaneous electrical activity of the
strip consists of high-amplitude discharges that occur on a generally reduced
level of electrical activity. Moderate hypercapnia suppressed these discharges.

Thus, the electrical activity of the strip we recorded is qualitatively
similar to the electrical activity recorded in the same species of animals by
other authors. We are inclined to explain the certain quantitative differences
(we observed spontaneous activity in the strip more frequently and for a longer
period of time) by the fact that we did not give Diplacin or an anesthetic to
the rabbits and we most frequently recorded the electrical activity of a strip
from the sensorimotor region.

The fact that anesthesia affects the electrical activity of a strip has /59
been noted by many authors, but the dependence of electrical activity on the
region of isolation has not been noted. By the way, in our experiments, the
electrical activity of the sensorimotor and parietal-occipital strips differed
somewhat. The activity of a strip from the posterior sections of the cortex
was less than that from the anterior, which is probably connected with the pe-
cularities in histological structure of different regions of the cortex.

An analysis of our data and the reference data shows that a cortical strip
from an unanesthetized animal has spontaneous electrical activity at least dur-
ing the first hours after isolation. All authors who have studied the electri-
cal activity of a strip consistently testify that it can react to electrical or
chemical stimuli. Certain data indicate an increased excitability of the strip,
the inhibiting influence from the subcortex disappears in accordance with the
general principle of an increase in the sensitivity of denervated structures
[Henry, Scoville, 1952]. Consequently, we can assume that a UHF field can af-
fect the electrical activity of an isolated strip.

The influence of the UHF field was applied by the same method as in the

preceeding series of investigations, i.e., the head of the rabbit was placed be-
tween the generator electrodes. The influence began 10-20 min after isolation
regardless of the character of spontaneous activity in the strip. Each exposure
lasted 2-3 min and was repeated every 20-40 min.

As before, prolonged reversible changes in electrical activity occurring dur-
ing the influence of the field were considered to be a reaction to the UHF field.
The electrical reactions of the strip differed in form from the reactions ob-
served in an intact brain and in isolated brain preparations. The basic series
of experiments conducted on 16 rabbits involving strip isolation in the sensori-
motor region showed that there were 51 reactions for 98 exposures (an average
stability of 52%). The average latent period was 27 sec.

j-y/^g/V\y'^V'*^^^^V*^'A'V';^V*'^

/ f-Z

nil

fz y I ^ ■ . • ' .' '

Figure 19. Electrical Activity in a Neuronally-Isolated Strip
of Rabbit Cerebral Cortex (1-2) Before (I) and During (II) the
Influence of a UHF Field of Weakly Thermal Intensity. Depend-
ing on the Initial Level During the Influence, the Electrical
Activity of the Strip Increased (A), Although the Electrocor-
ticogram of an Intact Hemisphere Either Did Not Change (3-4) ,
Decreased (B) or Acquired a Rhythmic Character (C) , Regardless
of Respiration (See the Curves Without Numbers). The Numbers
Designate the Leads for the Electrocortiogram (See the Dia-
gram) . Key: (a) 100 yv; (b) 1 Sec.

Of the 51 reactions, 32 were manifested as an Increase in activity (Figure
19, A), 12 as a reduction in electrical activity of the strip (Figure 19 ,B),
7 as the appearance of a regular rhythm of biopotentials with a frequency of
1-3 Hz (Figure 19, C).

During the influence on the electrical activity of the strip, we observed
either the appearance of high-amplitude discharges of different frequencies on
a background of suppressed electrical activity, or an increase in the frequency
of the high-amplitude discharges already present in the background. Sometimes
during the influence, the amplitudes of these discharges abruptly increased.
This reaction can be compared, to some degree, with the reaction of an intact
and isolated brain to a UHF field, and we can consider that during the influence
on any section of the brain, the UHF field brings about the generation of higher/61
amplitude potentials.

With the decrease in electrical activity of the strip, we saw either a sig-
nificant suppression of the potentials or their rarefaction. A similar reac-
tion was also encountered under the influence of a UHF field on sympathectomized
rabbits. To a lesser degree, suppression of the electrical activity character-
izes the influence of a UHF field, and it occurs, as a rule, on the altered
level of excitability of the CNS. This altered level is caused, in particular,
by the surgery.

The appearance of regular rhythms of electrical activity in the strip under
the Influence of a UHF field sharply demonstrates the presence of the field's
effect. This type of reaction did not occur in experiments involving an intact
brain or with isolated brain preparations. Only after damage to the hypothala-
mus in certain rabbits did we note the occurrence of "autonomic" rhythms in the
EEG that resembled the described rhythms of the strip in regularity. However,
the regular rhythms of the strip did not coincide with the respiration rate or
the frequency of cardiac contractions. It turned out that the appearance of
regular rhythms is not a specific reaction of the strip to a UHF field. In cer-
tain cases, M. A. Aladzhalova (1962) recorded a rhythm of 3 Hz in a rabbit cor-
tical strip during an interval from 15 to 25 min after isolation. A rhythm of
1 Hz, which was equal to the respiratory rhythm, but did not coincide with it
in phase, sometimes occurred 25 min after isolation. Such rhythms can be caused
by electrical or chemical stimulation of the strip.

Thus, although the forms of the change in electrical activity of an isolat-
ed cortical strip to a UHF field differ from those of an intact cortex, in both
cases we have a nonspecific change in electrical activity, which can also occur
under the influence of other stimuli.

More definite changes in the electrical activity of the isolated cortical
strip, of a qualitative character in comparison with the gradual quantitative
changes of the EEG under the influence of a UHF field on an intact brain, allow
us to more precisely determine the latent period of the reaction and the dura-
tion of the aftereffect, and also to reveal a new type of reaction that appeared
after the generator was turned off. The fact that the latter reaction sometimes
appeared in the absence of the usually observed reaction during the influence of
a UHF field (the basic reaction), and that it occurred following a definite la-
tent period, led us to the assumption that its cause is the switching off of the

field. The fact that the latent periods of the basic reaction to a UHF field
did not exceed 90 sec, and that the exposure usually lasted another 90 sec, dur-
ing which time the reactions did not occur, repudiates the assumption concern-
ing the possibility of a delayed basic reaction that occurs after the generator
is turned off. Therefore, we assumed that, besides the basic reaction, in an /62
isolated strip there is another electrographic reaction to the turning off of
the field. In its form, the reaction to turn-off was similar to the basic re-
action; it occurred after approximately the same latent period after turn-off
as the basic reaction did after turn-on of the generator, but the stability of
the reaction to turn-off was approximately 5 times less than the stability of
the basic reaction.

We saw the analog of
these facts in the electro-
graphic reactions observed
during the influence of
light. In our terminology,
the on-effect is the basic
reaction, and the off-ef-
fect, the reaction to turn-
off, is especially clearly
elicited in tests with an
isolated cortical strip, /63
but it can also be observed
under the influence of an
electromagnetic field on an
intact cortex. A more de-
tailed analysis of the re-
action to turn-off will be
given sometime later.

The distribution of
the latent periods in the
reactions of an isolated
cortical strip (see Figure
20, D), show that half of
the reactions occur 15 sec
after the beginning of the
exposure to the UHF field,
and one fourth occur after
5 sec. It turned out that
during the average latent
period (27 sec) , the iso-
lated strip is the most sen-
sitive formation to the in-
fluence of a UHF field,
leaving the intact brain
far behind and far exceed-
ing the isolated brain prep-
aration. Also, in the degree of stability of the reaction, the strip signifi-
cantly differs from an intact brain (52% versus 45%) . The aftereffect period
for the strip (1-5 min) was less prolonged than the aftereffect for an intact
brain (10-15 min).
52

5 Zi M

8A
(b)

//5 itib S W Bb

Bejiwiuid nanieHWHOdu iwpacSa, Cec-

ils , 165

Figure 20. Latent Period Distribution Curves for
Electrical Reactions to a UHF Field in Normal Rab-
bits (B) , an Isolated Brain Preparation (C) , a
Neuronally-Isolated Cortical Strip (D) , and the
Average Curve for All the Experimental Material
(A). Key: (a) Number of Cases, %; (b) Length
of the Latent Period, Sec.

It should be noted that these figures characterize the reaction of a corti-
cal strip isolated in the sensorimotor region, and we recorded the EEG reactions
to a UHF field in an intact and isolated brain from the occipital and parietal
regions. On the other hand, we have previously noted that spontaneous activity
m a strip isolated in the sensorimotor region predominates over spontaneous
electrical activity in a strip in the parietal-occipital region. It is possible
that the different cortical regions react differently to a UHF field. We have
previously shown that in an intact cortex, the reaction to a UHF field occurs
most clearly in the occipital region. Is this correspondence preserved after
isolation of a cortical strip? To answer this question we isolated a strip in
7 rabbits in just the sensorimotor region (47 exposures), and in 6 rabbits in
just the parietal-occipital region (44 exposures). The strips were identical

m sxze.

Since the certain tendency towards an increase in reaction stability (55%
versus 50%) and a reduction in the latent period (24 sec versus 26 sec) observed
in strips isolated from the sensorimotor region is unreliable, the reactions to
a UHF field of cortical strips isolated in different regions can be considered
identical.

The reaction stability of an isolated strip in the sensorimotor region to
a UHF field varied in different rabbits from 25 to 90% (in normal rabbits from
25 to 75%). Consequently, isolation of the strip did not exclude the individual
characteristics of the animals during the reaction to the UHF field. Therefore,
a comparison of the reactions of the cortical strips isolated in different re-
gions should be conducted on one animal. In connection with complications in-
volved in recording the electrical activity, which was expressed in the fact
that the recording was conducted simultaneously from two strips isolated in one
hemisphere, we immobilized the animals by injecting Diplacin, and put them on
artificial respiration, although we knew that such a procedure somewhat reduced
the sensitivity of these isolated cortical strips.

This series of experiments was conducted on 9 rabbits (63 exposures) . It /64
should be noted that in only 9% of the cases did we observe an electrical reac-
tion in both strips under the same influence, and this reaction began, as a
rule, after different latent periods. At the same time, however, the average
characteristics of the reactions of strips isolated in different regions coin-
cided. The coincidence of the average reaction stability is complete (30 and
30%), but in the average latent period the strips in the parietal-occipital re-
gion somewhat lead the strips in the sensorimotor region (34 sec versus 39 sec).

Thus, the experiments in which we recorded the electrical activity of two
strips isolated in one hemisphere under different conditions, support the con-
clusion that the electrical reaction to a UHF field is identical in cortical
strips isolated in the sensorimotor and parietal-occipital regions.

Consequently, in the future, with justification, we will compare the elec-
trical reaction to a UHF field of an isolated strip in the sensorimotor region
to the reaction of an intact and isolated brain from which we recorded the EEG
of the parietal or occipital regions .

In discussing the results of this series of experiments, we must call your

attention to the fact that Isolation of the strips (or one strip in two experi-
ments) does not occur without any effect on the intact hemisphere. After iso-
lation of a strip, the spontaneous electrocortlnograms in the adjacent hemisphere
change towards a predominance of slow, high-amplitude oscillations? and the EEG
reaction to a UHF field, although it preserves its qualitative characteristics,
i.e., is manifested as an increase in amplitude and a decrease in the frequency
of biopotentials, does occur with a smaller latent period (39 versus 53 sec).
In this case, the average reaction stability is reduced insignificantly (40%
versus 45%). Thus, the operation of isolating a cortical strip increases the
sensitivity to a UHF field not only in the strip itself, but also in the adja-
cent intact hemisphere. However, we intend to give a more detailed characteri-
zation of the excitability of a brain, sensitized by isolation of a strip, some-
what later.

It should be noted that, in contrast to the preceding tests, in recording
the electrical activity of the strip the electrodes directly contact the menin-
ges. Consequently, if the current fed to the electrodes has a stimulating ef-
fect, then the EEG reaction must be more marked than in the case of fastening
the electrodes to the bone, which offers greater resistance. However, in sever-
al control experiments it was shown that trepanation and recording of the elec-
trocorticograms did not change the electrical reaction of an intact brain to a
UHF field either in stability, or in the latent period.

Let us return to the reaction of an isolated strip to a UHF field. If it
depends little upon the place of isolation, how can it change over the time that
passes from the moment of isolation? As material to investigate this question, /65
we offer a series of experiments on 16 rabbits in which a strip has been isola-
ted in the sensorimotor region. Table 9 gives the data on the reaction stabil-
ity of a strip to a UHF field for each hour that passed from the moment of iso-
lation.

TABLE 9. DEPENDENCE OF THE STABILITY OF THE RE-
ACTION TO A UHF FIELD ON THE TIME THAT PASSED FROM
THE MOMENT OF ISOLATION OF THE CORTICAL STRIP.

Index

Time of existence of the iso-
lated cortical strip, hours

(0
4J
O

number of exposures
number of reactions
stability, %

24
11
46

34
16
47

100

98
51
52

The tabular results show that during the first three hours after isolation,
the reaction stability held stably at 46-47%. During subsequent hours, the sta-
bility increased to 60-100%. The conclusion regarding the increase in sensitiv-
ity as the time of existence of the strip increases is retained when we con-

sider the smaller number of exposures during each hour in the later hours. If
we total the results for the 4th, 5th and 6th hours, for 20 exposures we will
obtain 13 reactions Ca stability of 65%), while for the 3rd hour we observed 8
reactions for 17 exposures (a stability of 47%). However, during chronic tests
(2-10 days after isolation), the strip reacted to a UHF field less well than
during the first hours after the operation.

Thus, on the day of the operation, the reaction of a cortical strip to a
UHF field depends on the time of existence of the strip, but it does not depend
on the isolation site (within experimental limits). How can we explain this
last circumstance? Let us recall that in an intact brain, the occipital region
reacted to a UHF field better than the sensorimotor region. Consequently, the
reaction of an intact cortex to a UHF field is determined not only by the prop-
erties of the cortex itself (otherwise, the reaction would have been identical
in all regions) , but also by its connections with the lower-lying levels of the
CNS. In other words, a comparison between the reactions of an intact and an
isolated cortex shows that under the conditions involving an entire brain, a
UHF field acts not only on the cortex.

Does an isolated cortical strip react to a UHF field absolutely indepen-
dently, or does its behavior depend on the integrity of the lower-lying struc-
tures? To answer this question, we investigated the reaction of a strip to a /66
UHF field after sectioning at the level of the spinal cord or the midbrain. We
should note that spontaneous activity in the strip was reduced after sectioning
at the stated levels. Working with an isolated cat brain preparation. Burns
(1958) concluded the absence of spontaneous electrical activity in an isolated
cortical strip. At the same time, acute changes did not occur in the reactions
of a strip to a UHF field. Table 10 gives the results of experiments on the
electrical activity of an isolated cortical strip in the sensorimotor region of
an intact brain, and also after sectioning at the level of the spinal cord or
the midbrain.

TABLE 10. STABILITY AND LATENT PERIOD OF THE REACTIONS TO A UHF FIELD
OF A NEURONALLY-ISOLATED CORTICAL STRIP IN AN INTACT BRAIN, AFTER SEC-
TIONING OF THE SPINAL CORD AND AFTER SECTIONING OF THE MIDBRAIN.

Subject of the
investigation

Number of
animals

Number of
exposures

Number of
reactions

Stability,
%

Average la-
tent period,
sec

isolated cortical

strip in an intact
brain

isolated cortical

strip after sectioning
of the spinal cord

isolated cortical

strip after sectioning
of the midbrain

The tabular data show that the reaction stability in an isolated cortical
strip did not change after sectioning at the level of the spinal cord or the
midbrain. These facts indirertly testify that we observed an independent reac-
tion of the cortex to a UHF field. However, the increase in the average latent
period of the reaction of the strip after sectioning at the level of the mid-^
brain indicates a certain effect of the subcortical structures on the excitabil-
ity of the isolated strip. N. A. Aladzhalova (1962) also noted a similar reduc-
tion in the excitability of a neuronally-isolated cortical strip after intra-
collicular sectioning of the brain stem. She pointed out the possible role of
the humoral mechanism in" the realization of the influence of certain subcortical
formations on the excitability of the cerebral cortex. The general conclusion
is that an isolated cortical strip reacts identically (in the degree of stabil-
ity) regardless of the localization of the strip or of certain additional de-
struction to the integrity of the brain.

Discussion

/67

Summing up the results of experiments on the influence of a UHF field on
animals, we must note that the electrographic method has become a sufficiently
convenient approach to characterize the direct effect of an electromagnetic
field on the central nervous system. Numerous works show the existence of a
reflex path of the influence of a UHF field on the CNS , but this path has re-
mained outside the limits of our analysis, not because we ascribe small impor-
tance to it, but only because a different path (the direct effect) has only been
noted, but not analyzed in most works. It can be affirmed that, in the long
run, the reaction of an organism to a UHF field is determined by both the reflex
and the direct influences, and that in each specific case (depending on the in-
tensity, localization, etc.), one or the other path predominates.

Certain differences in our results from the results of other authors can be
explained by the fact that our influence lasted no more than 3 min and was re-
peated no more often than 20 min, when, as we have assumed, the organism has
finished reacting to the preceding influence.

What are the properties of the reaction we have investigated?

First, its appearance carries a statistical character. It is impossible to
predict the appearance of the reaction, but we can expect that of 100 exposures
on the head of an intact animal, 50 will elicit a reaction.

Second, the reaction depends on the individual characteristics of the ani-
mal, and on its initial functional state.

Third, the reaction depends on the intensity of the influence: the more
intense the field, the more stable the reaction.

Fourth, the reaction is most frequently manifested as an increase in elec-
trical brain activity.

Fifth, the reaction has an aftereffect and an off-effect.
56

Sixth, the reaction has a definite latent period.

With sufficient persuasiveness, these points indicate the presence of an
EEG reaction to a UHF field.

If we summarize the results of the basic series of experiments with respect
to the average stability and the average latent period of the reactions (Table
11), one can clearly see that the response will depend on the type of surgery.

TABLE 11. STABILITY AND LATENT PERIOD OF THE REACTION TO A
UHF FIELD IN RABBITS, BOTH CONTROL AND AFTER VARIOUS SURGERY.

Test conditions

Number of
animals

Number of
exposures

Number of
reactions

Stability,
%

Average la-
tent period,
sec

normal rabbits (con-
trol)

280

130

destruction of the
visual analysor

destruction of the
auditory analysor

destruction of the
olfactory analysor

partial desympathiz-
ation

destruction of the
hypothalamus

sectioning of the
midbrain

sectioning of the
midbrain and the
olfactory brain

strip of the sensimo-
tor cortex

145

strip of the parietal-
occipital cortex

cortical strip iso-
lated after sectioning
of the spinal cord

cortical strip iso-
lated after sectioning
of the midbrain

total

127

915

441

As the tabular data show, 127 rabbits were given almost 1,000 exposures
(915) ; in half of the cases (48%) , we observed a change in electrical brain ac- :
tivity. The spread of the average reaction s Lability was within the interval
from 217. (after destruction of the olfactory analyser) to 83% (after destruction
of the hypothalamus). However, most of the experimental groups showed a reac- lb§_ .
tion stability of about 50%.

We have tried to formally analyze the reaction mechanism in discussing the
latent period distribution curve. The general averaging of the distribution
curve is given in Figure 20, A. It is the result of an analysis of 391 reac-
tions in 95 rabbits, of which 37 rabbits were control, 10 were deafferentated,
6 had their sympathetic ganglia destroyed, 8 had their hypothalamus destroyed,
10 had their midbrain sectioned, 8 had their midbrain and olfactory brain sec-
tioned, and 16 had a neuronally- isolated cortical strip in their sensorimotor
region.

We should remember that the changes in the stability of the EEG reaction Jt^
to a UHF field, and in its latent period, do not always correlate with each oth-
er. Thus, after sectioning the olfactory brain in normal rabbits, and in a cer
veau isole preparation, the reaction stability decreased almost twice, but the
latent period did not change. After damage to the hypothalamus, the stability
increased almost twice, but the latent period did not change. On the other
hand, after isolation of a cortical strip, the stability remained practically
unchanged in comparison with the norm, but the latent period decreased by a fac-
tor of two. It is possible that the latent period characterizes excitability,
while the reaction stability characterizes the reactance of those structures
that react to a UHF field.

The obtained average empirical curve of the distribution of latent periods
of the reactions can be formally described by 3 theoretical curves of normal
distribution with a mode at 15 sec, 40 sec and 85 sec, wherein the 1st group
contains 27% of the reactions, the 2nd, 61% and the 3rd, 11% (Figure 20, A).

If we compare the total average curve with the distribution curve of the
latent periods for normal rabbits (Figure 20, B), we will observe a similarity
in the 2nd and 3rd groups of reactions.

These two groups of reactions are observed in one and the same rabbit, but

sometimes the change in biopotentials occurs at the 40th and at the 90th seconds

even under identical application of a UHF field. Consequently, the two possible
reaction mechanisms can develop independently of each other.

Analyzing the latent period distribution curve only for reactions of nor-
mal rabbits, we cannot say anything about their hypothetical mechanism except
to indicate its existence. But when we have examined the latent period distri-
bution curves for reactions of rabbits after different types of damage, certain
suppositions arise regarding the possibility of the origin for different groups
of reactions. The following discussion proceeds from the assumption that the
connections between different sections of the CNS during the formation of the
EEG reaction to a UHF field are accomplished only along nerve pathways.

Since the reactions of the 3rd group (an average latent period of about

sec) were absent after sectioning at the level of the midbrain (Figure 20 ,C),
we can assume that this group of reactions is induced by certain structures lo-
cated below this level. It is known that the basic mass of afferent pathways
pass into the brain below the level of sectioning. Consequently, if the reflex
pathways play some role in the formation of these reactions, their share is not
great (18% for normal rabbits and 11% for all the experimental material) . It is
also not necessary to reject the possibility of a direct influence of the UHF
field on the lower- lying sections of the CNS.

The first group of reactions (an average latent period of 15 sec) are par-
ticularly clearly delineated in the reactions of an isolated cortical strip
(Figure 20, D). We can assume that the cortex reacts directly to a UHF field /70
with a short latent period. However, this conclusion pertains only to isolated,
cortex, the excitability of which is increased. In normal rabbits, we did not
observe reactions with this short latent period. Does this mean that a normal
cortex does not react to a UHF field? We assvmie that the reaction of a normal
cortex takes place with a longer latent period than the reaction of isolated
cortex, and that the telencephalon and diencephalon, reacting simultaneously as
one total system, give the reactions (with an average latent period of about 40
sec) that make up the majority in both normal rabbits (82%) and in the average
distribution curve of the latent period of the reactions of most experimental
animals (61%) .

We shall return to the discussion of the question regarding the participa-
tion of different sections of the brain in the reaction to electromagnetic
fields when we discuss the results of tests with electrodes embedded in certain
sections of the brain.

Conclusions

1. The character of the EEC reaction of rabbits after a 30-60-sec exposure
to the influence of a 5,000 v/m UHF field on the head, depended on the method of
securing the animal. If the forelegs and ears were placed between the elec-
trodes, we noted a decrease in the biopotential amplitude on the EEC. If the
legs were secured along the spine, and the ears tightly bound to the head or
cut off, we noted an increase in the amplitude on the EEC. In the first method
of securing the rabbit, the UHF field caused motor reactions and cries from the
animal. The influence of a strong UHF field on the head sometimes caused auto-
nomic reactions: a quickening of respiration and abundant salivation. The mo-
tor and autonomic reactions, and also the EEC reactions consisting of a decrease
in the biopotential amplitude, can be explained by the thermal effect of the UHF
field on the extremities and ears, since such reactions also occurred during bi-
lateral external heating of the head region with the aid of two reflectors.

The EEC reactions manifested as increases in biopotential amplitude were caused
by the influence of the UHF field on the head region, and not by external heat-
ing. The stability of the EEG reaction was 82%, and the aftereffect lasted
about 20 min.

2. During a 3-minute exposure to a 1,000 v/m UHF field on the head of a
rabbit, we managed to record only EEG changes. Motor, vocal and salivary reac-
tions were not observed. The pulse and respiration rates did not change. The

EEG reaction to a UHF field occurred only during the influence on the head, and
it was most frequently manifested as an increase in biopotential amplitude and /71
a decrease in biopotential frequency, and also as an increase in the number of
spindle-shaped oscillations. The reaction had a diffuse character, but it was
most clearly observed in the occipital region. The reaction stability varied
around 45%, and the aftereffect lasted 10-15 min. The latent period of the re-
action varied from 15 to 115 sec, but in most cases it was equal to 40 sec. Af-
ter the generator was turned off, we observed changes in the EEG similar to the
off-effect. In our experimental conditions, we did not manage to observe either
adaptation or summation as a result of repeated exposure to the UHF field. The
excitability of the cortical end of the visual analysor, determined by the meth-
od of the reactance curve, was increased (in comparison with the normal) during
and one minute after the influence of the UHF field. The reaction stability in
this case attained 88%,

3. The EEG reaction to a 1,000 v/m UHF field was retained after destruc-
tion of the visual, auditory or olfactory analysors, after removal of the supe-
rior sympathetic ganglia, and after destruction of the hypothalamus, thalamus
or the reticular formation of the midbrain. The electrical reaction to a UHF
field in an isolated brain preparation, elicited as a result of sectioning at
the level of the midbrain, and in a neuronally-isolated cortical strip, was more
clearly noted than in an intact brain.

4. The change in electrical brain activity during the influence of a UHF
field can occur as a result of the direct effect of the field on the brain tis-
sue.

CHAPTER 2. THE EFFECT OF AN SHF FIELD ON THE ELECTRICAL
ACTIVITY OF RABBIT BRAIN

The dm, cm, and mm ranges of radio waves or microwaves, or superhigh fre-
quencies (SHF) , are next to the UHF range on the scale of electromagnetic oscil-
lations. Therefore, it is not surprising that many investigators have indicated
the significant similarity in the biological effect of UHF and SHF. We should
note, however, the higher thermal effect and the increased possibilities of an
acute local effect from an SHF field. At the present time, the biological ef-
fect of an SHF field has been studied more intensively than the biological ef-
fect of a UHF field.

A detailed survey of the questions connected with the biological effect of
a UHF field is given in recent works [Presman et al. , 1961; Presman, 1964b, c; 111
Gordon, 1960, 1964; and others] and in the collections "Concerning the Biologi-
cal Effect of a Superhigh Frequency Field" (1957) , "Concerning the Biological
Influence of Superhigh Frequencies" (1960), "Biological Effects of Microwave
Radiation" (1961) , "Concerning the Biological Effect of Radio-Frequency Electro-
magnetic Fields" (1964) and "The Biological Effect of Ultrasonics and Superhigh
Frequency Electromagnetic Oscillations" (1964) .

The Effect of an SHF Field on the CNS

People who work under the influence of an SHF field usually complain about
increased fatigability, periodic or constant headaches, extreme irritability and
somnolence [Brogichina, 1960; Sadchikova, 1960; Gembitskiy, 1962, Osipov et al. ,
1962; Tyagin, 1962; and others]. Prolonged exposure to an SHF field causes a
number of nonspecific reactions in man, among which the CNS reactions that occur
as asthenic states occupy the prime position. Autonomic nervous system changes
are manifested as a predominance of vagotonic reactions, hypotonia, bradycardia
and a change in the conductivity of the heart. Furthermore, there is a reduced
sensitivity of the olfactory analysor [Lobanova, Gordon, 1960] and the appear-
ance of irregular slow waves in the tracing of the cortical electrical activity
[Sinisi, 1954; Drogichina et al., 1962; Klimkova-Deycheva and Rot, 1963;
Ginzburg, 1964; and others].

Investigations on the effect of microwaves on the nervous system were con-
ducted on rabbits during exposure of the head to SHF fields of high intensity
[Olendorf, 1949]. A 3-minute exposure caused the animals to refuse food. Mor-
phologic changes in the gray and white matter of the brain were observed. Total-
body exposure led to death.

Exposure of the rat occiput to an SHF field led to convulsions [Austin,
Horwath, 1949, 1954]. The onset of convulsions was preceded by an increase in
brain temperature up to approximately 40''C.

Using the conditioned reflex method, it was shown that, during single and
repeated exposure of dogs to centimeter waves of different intensities, stimula-
tion of the higher nervous activity was observed following small doses, and

suppression was observed after large doses [Subbota, 1957, 1958]. The character
of the changes was determined by the Initial functional state of the CNS and by
the characteristics of the individual animals.

Investigation of conditioned and unconditioned salivary reflexes in dogs
after single and repeated exposures to low-intensity decimeter waves revealed
wave-like shifts in the conditioned reflex activity, and disturbance of the power
ratios after repeated exposure [Svetlova, 1962]. Immediately after a single /73
unilateral exposure, there was suppression of positive conditioned reflexes on
that side and insignificant reinforcement on the other side. On the next day,
suppression was noted on the opposite side. Compensating and paradoxical phases
frequently occurred. Differentiation was sometimes disinhibited. The uncondi-
tioned reflexes changed to a lesser degree and in a different direction. After
repeated exposure, a typical neurotic state developed in certain dogs. Follow-
ing a break in the exposure, normalization usually occurred in the functioning
of the cerebral hemispheres. Prolonged exposure caused adaptation in some ani-
mals and sunrmation in others.

Following chronic exposure to low- intensity microwaves, conditioned motor
reflexes in rats underwent phase changes [Lobanova, Tolgskaya, 1960; Lobanova,
1964], Following the initial exposures, there was an increase in the excitabil-
ity of the cerebral cortex and a weakening of the inhibition process. A lessen-
ing of excitability and a decrease in efficiency of the cortical cells was ob-
served as the number of exposure sessions was increased. After cessation of
the exposures, the changes in the conditioned reflex activity exhibited a wave-
like character, and full normalization was observed towards the end of the sec-
ond month.

On the day following exposure to high- intensity centimeter waves, mice ex-
hibited a decrease in the magnitude of their conditioned reflexes, partial dis-
inhibition of differentiation, and disturbances of the power ratios found in the
cerebral cortex [Gorodetskaya, I960].

It has been noted [Fleming et al. , 1961] that when rats are exposed to an
SHF field, their thermal regulation system and previously developed conditioned
reflexes are destroyed. The same authors noted that an SHF field increases the
motor activity and causes insomnia in simians.

Thus, the data obtained by the conditioned reflex method testifies to the
presence of an effect of an SHF field on the higher-level functions of the
brain. The changes in brain activity during exposure to microwaves resemble the
biological effect of a UHF field. A phase effect of an SHF field on the func-
tions of the cerebral cortex, as well as a dependence of the reaction on the
type of higher nervous activity of the animal and on the initial functional
state are also observed. It is possible that the described changes in condi-
tioned reflex activity are a nonspecific reaction of the brain to any injurious
factor. In any case, ionizing radiation causes similar changes in conditioned
reflex activity [Livanov, 1962].

When the head of an anthropoid was placed in a cylindrical resonator fed by
a 100-w generator operating in the 225-339 mHz range, a phase reaction was /74
noted in the animal [Baldwin, et al.,1960]. If the animal's chin was elevated,

exposure for 1 mln sequentially caused excitation, somnolence and destruction of
sensitivity. A 3-minute exposure frequently led to convulsions, terminating in
death. With the chin lowered, a 3^ninute exposure led only to excitation and
somnolence, and when the head was unsecured, these changes were not noted, but
the animals did position their heads upwards. A definite orientation in the
field was also noted in rats and dogs. The latter turned their heads toward the
microwave source. Exposure of the entire body except for the head did not cause
the noted reactions.

A slowing down of the frequency and an increase in amplitude was observed
in the electrical activity of the cortex of anthropoids when only the head was
exposed. The body temperature did not change substantially. All the changes
noted disappeared 24 hours after the test. The authors consider the described
reactions as phenomena of nonthermal origin occurring as a result of disturbance
in the normal functions of the dlencephalon and the midbrain, due to intracellu-
lar molecular changes.

Studying the reactions of the peripheral system to microwave exposure dur-
ing an increase in body temperature induced by a metallic heater or infrared
lights, certain investigators [McAfee, 1961, 1962; McAfee et al. , 1961; Seth,
Michaelson, 1964] concluded that an SHF field only causes a thermal effect. Re-
gardless of the method of heating, in cats they noted an Increase in the respi-
ration rate, pupillary dilatation, an increase in blood pressure, tachycardia,
etc., when the temperature of the peripheral nerves (radial, trigeminal or sci-
atic) reached 46*'C.

On the other hand, in studies on the effect of an SHF field on the func-
tions of the CNS, or on the entire organism, a concept regarding the nonthermal
character of the effect of an SHF field has been expressed [Bychkov, 1962; Gor-
don et al., 1962; Presman, 1962; and others].

By eliminating the cathode-electrotonic syndrome of functional changes or
the syndrome of cathode depression involving sharply expressed inhibition at a
low level of lability, anodizing the brain had a normalizing effect on animals
exposed to thermal intensities. The nonthermal influence of microwaves was
characterized by anode-electrotonic changes Involving suppression at a high
level of lability. Under these conditions, a dc cathode had a normalizing ef-
fect [Bychkov, 1962].

An SHF field of thermal intensity reduced the resistance of mice to strych-
nine poisoning, but a field of nonthermal intensity increased it [Bychkov, 1961].

Under the local Influence of an SHF field, anesthetization practically /75
eliminated the negative chronotropic effect on the cardiac rhythm. It is as-
sumed that this effect has a regular nature, and that a positive chronotropic
effect is Induced by the direct influence of the field on the brain cells [Livi-
tina, 1964].

During the influence of an SHF field, a disturbance was noted in the be-
havior of anteaters, who lost their ability to "inform" other anteaters about a
food source. During the influence, the anteaters oriented their snouts along the
force lines of the field [Jaski, I960].

A. N. Frey [1962, 1963] described the auditory sensations of certain peo-
ple under the influence of an SHF field as a buzzing, clicking orawhlRtle= An- -
tinoise plugs increased the SGnsitlvity to the field. According to this index,

2
the sensitivity to a pulsed SHF field was 3 pw/cm . The sound disappeared only

during screening of the temporal region of the head. Frey proposed that the SHF
field acts directly on the auditory nerve or on the brain cells by means of in-
teraction with the electrical and magnetic fields formed around the nerve cells.

There is also the assumption that the nonthermal effect of an SHF field de-
presses the synaptic transfers of impulses [Bychkov, 1962], due to the effect on
the activity of acetylcholine. It has been observed [Nikogosyan, 1960, 1964;
Bychkov, Syngayevskaya, 1962] that the activity of cholinesterase in the brain
is reduced under the influence of an SHF field.

A change has been noted in the epileptoid reaction of rats, which are sen-
sitive to sound stimulation, during the influence of microwaves of different
ranges [Kitsovskaya, 1960, 1964]. A decrease in the severity of convulsions, an
increase in the latent period and a change in the duration of the inhibition
period between the first and second excitation waves have also been observed.
In brief, an SHF field reduces the excitability and weakens the inhibition pro-
cess.

An increase in the potassium chloride content and a decrease in the glucose
requirement have been established in rats during the chronic influence of centi-

2
meter waves with an intensity of 10 mw/cm . An increase in the calcium require-
ment (appetite) was observed in rats that were subjected to the influence of

2
centimeter and decimeter waves with an intensity of 40 mw/cm [Kulakova, 1964].

2
During exposure to strong SHF fields (40-100 mw/cm ) , changes were noted in

the interneuronal junctions (synapses) of the cerebral cortex; these changes in-
cluded disappearance of the junctions at the dendrite tips of pyramidal cells.
During chronic exposure to SHF fields of different intensities, dystrophic
changes were noted in the neurons, especially in the cortex and in the thalamus-
hypothalamus region [Tolgskaya, Gordon, 1960, 1964]. Sometimes a productive re-
action of the glia, especially the microglia, was observed [Dolina, 1961; Tolgs-
kaya, Gordon, 1964].

In studies on the effect of an SHF field on an organism, the electrical /76
brain activity has more frequently been recorded than in studies on the biologi-
cal effect of a UHF field. Apart from the already cited works of Baldwin et al.
(1960), Fleming et al. (1961) and clinical EEG investigations, we should mention
the works of M, S. Bychkov (1957, 1962), which especially investigated the ef-
fect of an SHF field on rabbit and cat EEGs. The EEGs were recorded before and
after exposure of the animals to microwaves of different intensities. Differ-
ently directed changes in the amplitude and frequency of cortical biopotentials
were noted. For brief exposure (10 min) , excitation was noted, but with a pro-
longed exposure, an inhibition effect was noted. The most expressive EEG chang-
es appeared on the treated side, which testifies to the direct effect of a UHF
field on brain tissue. On the other hand, neurodynamic changes also occurred
during local exposure on the extremities. This fact indicates the presence of

a reflex path for the effect of an SHF field on the CNS. Following the injec-
tion of novocain, the effect of local exposure on the extremities disappeared.

Simultaneously with our reports concerning the effect of an SHF field on
electrical brain activity [Bavro, Kholodov, 1962; Kholodov, 1962a, Kholodov,
Zenina, 1964], several investigations devoted to this question [Gvozdikova et
al., 1964a, b; Zenina, 1964] appeared, which testifies to the development of
electrographic methods for analyzing the biological effect of an SHF field.

We considered our problem to be a comparison of the electrical reactions of
an intact and an Isolated brain, and a cortical strip, to the influence of con-
stant and pulsed SHF fields of thermal and nonthermal intensities. Furthermore,
we proposed to compare the electrical reaction of the brain to SHF and UHF
fields.

The Effect of a Constant SHF Field of Thermal
Intensity on the Rabbit EEC

As an analysis of the works of Bychkov (1957) and the experiments of Bald-
win et al. (1960) show, the EEC reactions of different species of animals to the
influence of a UHF field were sufficiently polymorphous. It remains unclear
whether this poljraiorphism is explained by the species peculiarities of the ex-
perimental animals or by the applied field strength. Furthermore, these inves-
tigators did not quantitatively characterize the index of the reaction that we
call stability. Proceeding from these statements, we considered it necessary
to investigate the EEC reaction to an SHF field in one species of animal, using
one method and one treatment of the results, as was done in the study on the in-
fluence of a UHF field. Thus, the purpose of our work is a comparison of the
results of the local influence of UHF and SHF fields on the head of a rabbit. 777

As in the experiments involving a UHF field, we began with exposure to a
sufficiently strong SHF field, the calculated power flux density of which was

2
close to 1 w/cm . The field was created by a "Luch-58" generator, whose osc-
illation frequency was 2,400 MHz. The duration of the exposure varied from 1
to 5 min, but most frequently it was 3 min. Twenty rabbits were exposed 92

times. Furthermore, an SHF field with a power flux density of 100-300 mw/cm^
acted on the rabbit's head for the same duration. These 12 rabbits were exposed
120 times.

The EEC changes consisted of the appearance" of spindles in the sensorimotor
region and slow high-amplitude oscillations in the visual region (Figure 21).
Let us recall that the same type of EEG changes appeared under a UHF field, al-
though the appearance of spindle-shaped oscillations was noted less frequently.
The stability of the reaction to an SHF field of the applied intensities was
90%. The stability was identical for power flux densities of 200 and 1,000 mw/

2
cm , although the latent period of the reactions depended on the intensity of
the exposure.

As is evident from Figure 22, the application of stronger fields did not

-'V

^ ' ' . (b) ^ceH -

Figure 21. EEG Changes During the Influence of an SHF Field
of Thermal Intensity on the Rabbit Head. 1 = EEG of the Vis-
ual Cortex; 2 = EEG of the Sensorimotor Cortex. Key: (a)
200 yv; (b) 1 Sec.

cause reactions with a latent period that exceeded 1 min. Attention should be
called to the fact that a strong field caused a reduction in the average latent
period of the reactions only due to a decrease in the number of reactions with
a long latent period. The mode of the latent period distribution to strong and
weaker SHF fields occurred in the same interval, 25-35 sec. This fact indi- U°.
cates that the observed EEG reaction is triggered by such a slow mechanism
that it does not change its qualitative characteristics even when a stronger
SHF field is applied.

The Effect of an SHF Field on the Reactance Curve

A difference in the effect of a strong and a weak SHF field also occurred
when we determined the time for the appearance of assimilation to a rhythm of
light flashes of increasing brightness (the reactance curve). In 4 rabbits, we
determined 30 EEG reactions to interrupted light before, 1 minute after the
start of, and 1 minute after the end of an exposure to a field with a power
flux density of 100-300 mw/cm^. In 3 cases, the time for the appearance of as-
similation to a light rhythm under the influence of the field did not change,
in one case it increased, and in 26 cases it decreased. On the average, before
exposure, this time was 19.6 ± 0.3 sec; during exposure, 17.8 ± 0.3 sec; and Ul
after exposure, 19.5 ± 0.3 sec. Thus, an SHF field with the stated power flux
density increased the sensitivity of the cortical and visual analysors with sta-
tistical significance (p < 0.01). However, this effect was observed only dur-
ing exposure to the field: it disappeared 1 minute after the generator was
turned off. In this case the reaction stability was 87%, i.e., as both a trig-
gering (one that caused its reaction) and a correcting (one that changes the
reaction to stimuli of different modality) stimulus, the SHF field induced ef-
fects with identical frequency.

However, under the influence of an SHF field with a power flux density of
about 1,000 mw/cm^ on the rabbit head, the excitability of the cortical termi-
nations of the visual analysor was reduced. The following picture emerged from
the results of 25 exposures on 4 rabbits. The time for the appearance of as-
similation to light flashes remained unchanged only once during the influence
of the field and once after the generator was turned off. In all other cases,

J ' ■ •

' ' A

S Z5 ¥5 65 85 105 f25 m 165 185
(b ) SaiuvuMa nameHntHoeo nepuoia , cck

Figure 22. Latent Period Distribution

Curves of the EEC Reactions to an SHF

Field with a Power Flux Density of 1,000

2 2

mw/cm (1) and 200 mw/cm (2). Key:

(a) Number of Cases, %; (b) Length of

the Latent Period, Sec.

the time increased. Before the ap-
plication of the field, it was 18.4
±0.3 sec, during the influence of
the field, 22,2 ± 0.4 sec, and af-
ter the field was turned off, 21.3
± 0.4 sec. These data show that
during the influence of an SHF field
and 1 minute after the generator is
turned off, the excitability of the
cortical terminations of the rabbit
visual analyser decreased with sta-
tistical significance (p < 0.01).
Thus, like any other stimulus, an
SHF field has a phase effect on the
excitability of the CNS. A weaker
field heightens the excitability,
and a stronger field lowers it.

In this connection, we should
recall that fields with different
power flux densities did not differ
from each other in the type of the
spontaneous EEG changes they in-
duced; in both cases an increase in
the number of spindles and slow
high-amplitude oscillations of po-
tential were noted in the cerebral
cortex. Consequently, just one
tracing of a spontaneous EEG cannot
give reliable evidence regarding the
predominance of one or another basic
nervous process in the rabbit CNS.
The application of test stimuli is
necessary.

The Effect of an SHF Field on the Electrical Activity
of a^ Neuronally-Isdlated Cortical Strip

In discussing the mechanism of the effect of an SHF field on the CNS, cer-
tain investigators have made statements concerning the direct effect of the
field on the cerebral cortex [Bychkov, 1957; Presman, Levitina, 1962; and oth-
ers]. To check this hypothesis, we conducted a series of tests on 14 rabbits
bearing a neuronally-isolated strip in the sensorimotor region of the cortex on
the left hemisphere. The number of exposures was 119, the power flux density

2
was 200 mw/cm . The experimental methodology was the same as in the study on
the effect of a UHF field on the electrical activity of a strip. /80

It was shown that a strip can react to an SHF field with an increase in
electrical activity (Figure 23) . This type of strip reaction also predominated
during the influence of a UHF field. We should note that in this case, the

reaction of the strip was less frequent than the reaction of an Intact brain.
The reaction stability of a strip was 50%, and of an Intact brain, 90%.

2 yv'i.(wv>ori/v~vi4rvvv«Hi»-NAAAr/^^

2 .v4-..-,,vA-w-v'v.<' rv-~-.w^V{V|VJVj^ftW*^^

X.W « /' >"'' > l j

Figure 23. The Electrical Reaction of a Neuronally-Isolated
Cortical Strip and Adjacent Sections of an Intact Brain to an
SHF Field. A = Before Exposure; B = During Exposure; the Num-
bers Indicate the Electrocortlcogram Leads. 1 = Isolated Cor-
tical Strip; 2 = Visual Region of the Damaged Hemisphere; 3 =
Sensorimotor Region of the Intact Hemisphere; 4 = Visual Region
of the Intact Hemisphere.

The average latent period of the strip reaction was less than that of the
intact brain (20 ± 2 versus 63 ± 4 sec). In the latent period distribution
curve of the strip reactions, the mode was at 15 sec, while for the intact
brain it was at 35 sec (Figure 24). Consequently, although the reaction is ob-
served less often, an isolated cortical strip reacts to an SHF field more quick-
ly than an Intact brain.

Apart from spindles and slow waves, the change in the functional state of
the rabbit brain following mechanical damage to the cortex of one hemisphere in
order to Isolate a strip sometimes led to the appearance of convulsive discharg-
es on the EEC during the influence of an SHF field (Figure 23). We should note
that the high temperature of the air (the tests were conducted in the summer)
led to the appearance of convulsive discharges, which could also be elicited by
any uncontrolled stimulus, and were provoked by the next stimuli we adminis-
tered: light, sound, a constant magnetic field, and also an SHF field. A de- X£i
tailed comparative characterization of the EEC reaction to these stimuli will
be given later. Here, however, we want to note that the SHF field caused con-
vulsive discharges on the EEC more often than light or sound, and these dis-
charges were sometimes accompanied by convulsive twitching of the animal.

The latent period of convulsive EEC reactions did not differ from the la-
tent periods of other types of EEC reactions, although on the whole the damaged

(b)

rLJJ. ^^''^ ^^ "S <35 ii5 175 m
Be/ruvuHa mmeHniHOit nepuoda ce/t

brain reacted to the SHF field more
quickly than the intact brain. As is
evident from Figure 24, the mode for
the distribution of latent periods of
the damaged hemisphere reactions
falls at 25 sec.

It is interesting to compare the
latent period distribution curve of
damaged brain reactions to a 200 mw/

2
cm SHF field with the latent period

distribution curve of the reactions

2
of an intact brain to a 1,000 mw/cm

SHF field (Figure 22). These curves
coincide almost completely.

Two important conclusions follow
from this fact.

First, depending on the initial
functional state, the form of the EEG
reaction to an SHF field can change,
i.e., it can be expressed in convul-
sive discharges following mechanical
damage to the cortex. However, we
can assume that this reaction is ac-
complished by the same mechanism as
the reaction that is manifested as
an increase in the nvmiber of spindles or slow high-amplitude oscillations on the
EEG, since the latent period of the different reaction forms is identical. Sec-
ond, the reaction to an SHF field can be optimized by two methods: increasing
the power flux density of the field or increasing the sensitivity of the CNS.
In both cases, the ceiling for optimization of the reaction was identical. The
stability was 90%, and the average latent period was 25 sec.

The occurrence of a convulsive reaction to an SHF field after isolation of
a cortical strip allows us to separate the reaction to generator turn-off, /82
which can occur in the strip, the damaged hemisphere, the intact hemisphere, or
in all leads simultaneously.

In the damaged hemisphere, the reaction to turn-off was characterized by a
stability of 20% and an average latent period of 21 ± 2 sec; the latent period
distribution curve of the reactions to turn-off had a mode at 15 sec (Figure
25), and it resembled the latent period distribution curve of the strip reac-
tion to turn-on of the SHF field.

The reaction to turn-off was also observed in the intact brain, only there
it was encountered less often and was less distinct. The turn-off reaction co-
incided with the turn-on reaction (the basic reaction) in the form of the EEG
changes. Several seconds after the SHF generator was turned off, we noted an
increase in the number of spindles or slow high-amplitude oscillations. In

Figure 24. Distribution Curves of the
Latent Period of Electrical Reactions
to an SHF Field in an Intact Brain (1) ,
an Undamaged Hemisphere (2) and an Iso-
lated Contralateral Cortical Strip (3) .
Key: (a) Number of Cases, %; (b)
Length of the Latent Period, Sec.

5 tS 25 35 95 55 65
(\yye/iiii>u>ii mmeHmntti iefiLilt,eef

Figure 25. Latent Period Distri-
bution Curve of the EEG Reaction
to Turn-Off of the SHF Field.
Key: (a) Number of Cases, %; (b)
Length of the Latent Period, Sec.

contrast to the basic reaction, the turn-
off reaction was observed less often (in
20% of the cases versus 90%) . Souietlmea
the basic reaction was absent, but the
turn-off reaction appeared distinctly,
which forces us to assume a certain inde-
pendence of these reactions.

The Effect of a Modulated SHF
Field on the Rabbit EEG

It became clear that the rabbit CNS
reacts not only to the presence of fields,
but also to their change. In this case,
one would expect that a modulated field
would have a more expressed effect than a
constant field. To check this assvimption,
we conducted experiments in which we com-
pared the biological reaction of rabbit
cerebral cortex to an SHF field with a

2
power flux density of 200 mw/cm that was
constant or modulated at a frequency of 2,
5, 10 or 50 Hz. Ten rabbits were each
given five 3-minute exposures to an SHF
field modulated at each of these frequen-
cies. Each range of modulation was char-
acterized by 50 exposures. The obtained results were compared with the reac-
tion to a constant field influence.

We noted that a modulated SHF field causes EEG reactions that are the same
in form and stability as those caused by a constant field. A certain difference
was observed only in the latent periods. J—

Figure 26 gives the latent period distribution curves of the EEG reaction
to a constant SHF field and to the same field modulated at a frequency of 10 Hz.
As is evident from the figure, the EEG reactions to a modulated field occur ear-
lier than those to a constant field.

Figure 27 gives the curve of the dependence of the average latent period of
the EEG reaction to an SHF field on the modulation frequency. As the modulation
frequency increases, we observe a tendency towards shortening the average latent
period of the reaction. This dependence is not proportional. The largest dif-
ference is observed upon passing from a constant field to one modulated at a
frequency of 10 Hz. During transition from 10 to 50 Hz, the averages of the la-
tent period do not change, and upon transition from a constant field to one that
is modulated at a frequency of 2 Hz, the changes in the average latent period of
the reactions are insignificant.

Experiments involving a modulated field force us to assume that a discon-/84
tinuous field has a somewhat more expressive physiological effect on the rabbit

*S 65 85 105 125 m 165 185 205
(b) BpeuR , cen

Figure 26. Latent Period Distribution
Curves of the EEG Reactions of Rabbits-
to a Constant SHF Field (1) and to an
SHF Field Modulated at a Frequency of
10 Hz (2). Key: (a) Number of Cases,
%; (b) Time, Sec.

6 10 It to 22 ZS JO 3f 38 V2 ¥B iO
/■u\ Vacmoma Madymmuu ,fn

Figure 27. The Dependence of the Average Latent
Period of the EEG Reactions to an SHF Field on
the Modulation Frequency. Key: (a) Latent Peri-
od, Sec; (b) Modulation Frequency, Hz.

EEG than a constant field.
The effectiveness of low
frequencies (up to 10 Hz)
leads to the thought that
the possible coincidence
of the modulation frequen-
cy of the SHF field with
the frequency characteris-
tics of the electrical ac-
tivity in rabbit brains
plays an important role
here.

The Effect of Caffeine
on the EEG Reaction
of Rabbits During
the Influence of
a. Constant SHF
Field

As tests involving
isolated cortical strips
have shown, the electrical
reaction of the rabbit
brain to an SHF field de-
pends on the initial func-
tional state. For addi-
tional confirmation of this
conclusion we decided to
conduct tests involving the
influence of a field on the
head of a rabbit after pre-
liminarily increasing the
excitatory process. For
this purpose, we subcutan-
eously (in the thigh) in-
jected 18 rabbits with 1
mg/kg of caffeine. We be-
gan the experiments on the
effect of an SHF field 30
minutes after the injec-
tion. In 13 rabbits, we
observed the appearance of
convulsive discharges in
the EEG, and sometimes con-
vulsive twitching 1-2 min
after the start of the ex-
posure to an SHF field with
a power flux density of

1,000 mw/cm^ (Figure 28).

Ninety-two 5-minute exposures of the same strength on 20 rabbits that were not
injected with caffeine, did not induce one instance of convulsive discharges in
the cerebral cortex. Injection, alone, of the same dose of caffeine into rab-
bits did not cause the appearance of convulsive discharges in the EEC. Conse-
quently, only the summation of the physiological effects of an SHF field and of
caffeine could cause these changes in the rabbit EEC.

/>■....-,.. v....- ■•■■■■ ■ ■-— ^■^■'::.w.^v: ^

„,y,,^r

(a) 200ml!L.

_\_ ._ (b) ICBK :

Figure 28. The Appearance of Convulsive Discharges in the
Rabbit Cerebral Cortex During the Influence of an SHF Field,
After Preliminary Injection of Caffeine. 1 = EEC of the Vis-
ual Cortex; 2 = EEC of the Sensorimotor Cortex. Key: (a)
200 yv; (b) 1 Sec.

Thus, during the influence of relatively powerful SHF fields on the head
of a rabbit, we observed the appearance of slow high-amplitude oscillations, /85
spindles or convulsive discharges on the EEC, i.e., just the changes that char-
acterized the effect of a UHF field. The similarity of these effects also in-
cluded a long latent period of the reactions, a reaction to turn-off, and an
effect on the electrical activity of a neuronally- isolated cortical strip. We
also observed that a weaker SHF field increases the excitability of the cortical
terminations of the visual analysor (as determined from the reactance curve),
and a strong SHF field reduces it.

The similarity in the physiological effect of UHF and SHF fields, which has
been noted by many authors, was revealed in our experiments very distinctly.
However, in explaining the causes of this similarity, we must first assume the
possible thermal effect of these fields, since in our experiments we used ther-
mal and scarcely thermal field strengths. It was necessary to conduct similar
experiments with nonthermal SHF field doses.

The Effect of Pulsed SHF Fields of Thermal and
Nonthermal Intensity on the Rabbit EEC

We were given the opportunity of studying the biological effect of an SHF
field of nonthermal strength in the Laboratory of Electromagnetic Radio-Frequen-
cy Waves (directed by Z. V. Gordon) of the Institute of Labor Hygiene and Occu-
pational Diseases of the USSR Academy of Medical Sciences (directed by Professor
A. A. Letavet). This allowed us to compare our data with the results obtained

by the coworkers of this laboratory using different methods for investigating
the effect of an SHF field on t> a CNS of an animal. In contrast to our previ-
ous method of exposure, a pulsed SHF field (X = 52 cm) acted not only on the
head, but also on the entire body surface of the animal from one side. There-
fore, at the very start of the investigation, we asked about the possibilities /86
of a mechanism of effect that was different from the one we considered in our
study on the effect of UHF and SHF fields on the head of a rabbit. Proceeding
from this premise, we decided to investigate the effect of pulsed SHF fields of

2 2

nonthermal (2 and 10 mw/cm ) and thermal (50 mw/cm ) power flux densities on the

bioelectric activity of an intact and on an isolated rabbit brain. Isolation
was performed by sectioning at the level of the midbrain.

Total-body exposure was always conducted from the left side. Electroenceph-
alograms of the parietal regions of the cortex of both hemispheres were record-
ed with needle electrodes by the monopolar method. The EEGs were recorded on a
four -channel ink-writing VNIIMIiO electroencephalograph located in a room next
to the one used for exposure. Usually, we recorded a background EEG for 1 min
and, without interrupting the recording, applied a 3-minute dose, then recorded
another 1 min of cortical electrical activity after the exposure ended. The ex-
posure was repeated every 10-15 min. The tests were conducted on 14 rabbits,
who received a total of 300 exposures. SHF fields of each power flux density

2
(2, 10 and 50 mw/cm ) were applied 100 times; an intact brain was exposed 50
times, and an isolated brain preparation, 50 times.

Most frequently (76% of the total number of reactions), in response to any
power flux density on the EEG of the intact brain, we noted an increase in bio-
potential amplitude, which was sometimes accompanied by a decrease in biopoten-
tial frequency (Figure 29, A). Sometimes (20% of the cases), in response to the
exposure, we observed a prolonged desynchronization reaction (Figure 29, B) .
And, finally, convulsive discharges appeared on the EEG during the exposure
(Figure 29, C) , but only very rarely (4% of the cases). The EEG changes occur-
ring during an influence (especially the slow high-amplitude oscillations) last-
ed for several minutes after the influence ceased.

Thus, the form of the EEG reaction to a pulsed SHF field was basically sim-
ilar to the form of the EE^ reaction of rabbits to a constant UHF or SHF field.

We observed convulsive discharges in the intact brain during exposure only
two times in the same rabbit, and we explain this fact by the individual height-
ened excitability of this animal's CNS. We could obtain this type of reaction
to a constant SHF field by artificially increasing the excitability by an injec-
tion of caffeine, or by mechanical damage to the cortex. It is possible that
this rabbit would also react with convulsive discharges to the application of a
constant SHF field. In brief, we do not note any specifics of the effect of a
pulsed SHF field in this form of reaction.

The prolonged desynchronization during exposure is another matter. We /87
frequently saw this type of reaction during the influence of a powerful UHF
field on the extremities of a rabbit. It is possible that the method of total-
body exposure is the cause of the occurrence of this form of EEG reaction.
Since this form was encountered relatively rarely (4 times less often than the

. ..„ .... -J^vlU

4 tA/tH^Kf

2 /VVX^>.y^M/'-»'''^-

Figure 29. Forms of the EEG Changes During the Influence of a
Pulsed SHF Field on an Intact Rabbit Brain. A = Increase in
Potential Amplitude; B = Decrease in Potential Amplitude; C =
Appearance of Convulsive Discharges; 1 = EEG Before Exposure;
2 = EEG During Exposure. Key: (A) 100 yv; (b) 1 Sec.

Increase in biopotential amplitude) , we did not conduct a detailed analysis of
the paths of its origin.

The general conclusion is that constant UHF and SHF fields and pulsed SHF
fields of different intensities cause similar changes in the rabbit EEG.

This similarity appeared not only in the form of the basic reaction, but
also in the appearance of the desynchronlzatlon reaction at the moments the gen-
erator was turned on and off (Figure 30, A, B) . These desynchronlzatlon reac- /88
tlons frequently occurred with a latent period measured in fractions of a second,
and they lasted for 2-6 sec. They sometimes occurred during the exposure that
caused the basic reaction, but they could not appear Independently of the basic
reaction. The turn-on reaction could also be observed independently of the turn-
off reaction. The average of the first was 31%, of the second, 12%. The cer-
tain Independence of the described reactions made it possible to look for anoth-
er source for them, for example, the appearance and disappearance of sound when
the generator was turned on and off. However, the presence of similar reactions

during investigations of other EMF in which a sound stimulus was excluded, al-
low us to assume that, like any other stimulus, an SHF field causes a nonspecif-
ic EEG reaction (of the orienting type) at turn-on and turn-off.

B ' * ' » i ' ' ' ■ ■ '

*f\M,»A;.^y^v^^Jy,(^/^f AvMvMM; ^MM^\r\;^VV^iWM;Vi^^^/;-■, :k ■

(a) BUuMf^d [__

(b) h-s

Figure 30. EEG Reactions Occurring When the SHF Generator is
Turned on (A) and off (B) , and the Off-Effect, the Reaction to
Turn-Off (C, D) . The Arrows Designate the Moments the Genera-
tor is Turned on and off. Key: (a) 200 pv; (b) 1 Sec.

Concluding our description of the form of the EEG reactions during the in-
fluence of a pulsed SHF field, we must mention that a reaction resembling the /89
basic reaction in form (Figure 30, C, D) sometimes occurs after the field is
turned off. In other words, slow high-amplitude waves and spindles appeared on
the EEG 5-25 sec after the field was turned off. This reaction frequently oc-
curred when the basic reaction was absent. The turn-off effect probably appears
to a lesser degree in the background of the aftereffect from the basic reaction
and, therefore, the stability of the reaction to turn-off was 13%.

Thus, we noted the following general scheme of the EEG reaction of rabbits
to any UHF or SHF field. This includes changes in the EEG that occur when the
field is turned on and off (these are usually an insignificant decrease in the
biopotential amplitude and an increase in the biopotential frequency) , and chang-
es observed during the influence of the field and several seconds after it is
turned off (usually the appearance of slow high-amplitude oscillations of poten-
tial and spindles) . From the external picture of the change in cortical bio-
potentials, the reactions to EMF can be divided into two clear groups: the de-
synchronization reactions are faster, occuring with a latent period of less than
one second at turn-on and turn-off, and lasting not more than 10 sec; the syn-

chronization reactions are slower, occurring with a latent period of 5-90 sec
and lasting from 20 sec up to several minutes.

Since the EEG reactions of rabbits to external heating in our tests were
manifested as prolonged desynchronization, we assumed that the forms of the EEG
reaction to EMF that we have noted are essentially the result of a nonthermal
mechanism of the effect of EMF on the CNS.

The Effect of an SHE Field on the Rabbit EEG After
Sectioning at the Level of the Midbrain

The assumptions stated above needed support from the side of the physiolog-
ical mechanism of the effect of an SHF field of nonthermal intensity. Therefore,
the next stage of our investigations was a clarification of the question regard-
ing the direct effect of an SHF field of nonthermal intensity on the structures
of the forebrain and diencephalon. The experiments were conducted on an iso-
lated preparation, cerveau isol6.

f jyw^V ^ViA^*^""^"' ":

2 a-'«VvV(V'**wWJ**'**^^^^

(b) fct'/c i

Figure 31. Forms of the EEG Changes During the Influence of a
Pulsed SHF Field on an Isolated Rabbit Brain Preparation. A =
Increase in Potential Amplitude; B = Decrease in Potential Am-
plitude; C = Appearance of Convulsive Discharges; 1 = Before
Exposure; 2 = After Exposure. Key: (a) 100 yv; (b) 1 Sec.

Although the spontaneous EEG rarely changed towards predominance of slow
waves after the stated operation, the character of the change in the electrical
brain activity during exposure remained as before. In most cases (71% of all
reactions), we observed an increase in the biopotential amplitude (figure 31, A),
in 19% of the cases we observed its decrease (Figure 31, B) and in 10% of the
cases, convulsive discharges appeared (Figure 31, C) . In comparison with an in-
tact brain, there was a relative increase in the cases in which convulsive dis -/90
charges occurred in the cortex during exposure (10% versus 4%) .

The nimber of desynchronization reactions at turn-on and turn-off of the
field decreased to 3-4%, the number of basic reactions (stability) increased
from 30% to 55%, and the number of reactions to turn-off remained practically
unchanged (15% versus 13%) .

Thus, if we judge from the degree of stability of the basic reaction, de-
afferentation of the brain increases its sensitivity to a pulsed SHF field (as
to a UHF field), which indicates the important role of the direct effect of this
factor on the structures of the diencephalon and telencephalon.

The question arose as to whether the increase in reaction stability after
sectioning at the midbrain level can be explained by deafferentation or by the
stimulation induced by mechanical trauma. It seemed to us that an analysis of /91
the change in the reaction stability depending on the time that passed after
sectioning would help to answer this question to some degree. It was observed
that 1 hour after the operation, the stability of the basic reaction was 46%,
after 2 hours it had increased to 53%, and after 3 hours, it had reached 70%.
This fact forces us to assume that the basic cause of the increase in sensitiv-
ity of an isolated brain to an SHF field is deafferentation, and not just the
stimulation caused by trauma.

The Dependence of the EEG Reaction of an Intact and an
Isolated Brain on the Intensity of an SHF Field

Until now we have discussed general data on the effect of a pulsed SHF
field. It is time to investigate the dependence of the physiological effect on
the field intensity. We determined this dependence from the average stability
and the average latent period of the basic reaction. From Figure 32 it is evi-
dent that, as the intensity of the pulsed SHF field increases, the average la-
tent period of the basic EEG reaction of an intact rabbit brain decreases, but
its stability increases. If we combine the results on the effect of pulsed and
constant SHF fields with different wavelengths, we shall note that when the pow-

2
er flux density is from 2 to 200 mw/cm , there is an increase in the reaction

stability from 20 to 90%, and a shortening of the average latent period.

Finally, we showed that the intensity of the EEG reaction of the rabbit /92
brain increases as the intensity of the SHF field is increased, but the mathe-
matical character of this dependence is still not clear.

After sectioning at the midbrain, the average latent periods of the reac-
tions did not change significantly, and the reaction stability increased at each

30
80
W
SO
SO

30
20

Llll

BCD

field Intensity, but the weaker the
field, the greater the degree of
increase. Thus, at a power flux

2
density of 2 mw/cm , the stability

2
□/ increased 2.6 times, at 10 mw/cm ,

2
\^ 1.7 times, and at 50 mw/cm , 1.5

times. In other words, after sec-
tioning at the midbrain, we noted a
tendency towards equalization of the
stability of the reaction to a field
of different intensity. Many inves-
tigators have noted the inconstancy
of the physiological reactions to
the influence of electromagnetic
fields. In our tests, this proper-
ty of the reaction was manifested as
low reaction stability. In each
specific case, it was impossible to
predict whether a reaction would oc-
cur or not under the given influence.
As in the case of the UHF field, the
occurrence of a reaction could not
be explained by adaptation or summa-
tion. In some cases (Figure 33, A,
rabbit no. 1) we observed the pro-
cess of suiranation; in other cases (Figure 33, C, rabbit no. 3) we observed adap-
tation; and, in total, during exposure to nonthermal and thermal doses, the re-
action was randomly distributed regardless of the result of the previous influ-
ence. The statistical character of the EEG reaction to an SHF field probably
cannot be explained by just the weak nature of the stimulus, because we some-
times observed such a strong reaction as convulsive discharges. However, the
appearance of this form of cortical biopotential in a normal animal did not
change the latent period or the reaction stability. In other words, by itself,
the reaction could be very strong, but the triggering mechanism of the reaction
did not operate during each exposure. It is probable that the formations on
which the EMF act are responsible for this triggering mechanism. The possibil-
ity of a direct effect of an SHF field on the structures of the diencephalon and
telencephalon allows us to assume that just these structures, which are not spe-
cialized receptors, bear the justification for the statistical character of the
reaction. Consequently, by affecting these structures in some manner, we can
increase the reaction stability.

Figure 32. Dependence of the Average
Latent Period (1) and Stability (2) of
EEG Reactions on the Intensity of the

SHF Field. A = 2 mw/cm

C
mw/cm

50 mw/cm
2

200 mw/cm

10 mw/cm ;
E = 1,000

The Effect of Caffeine on the EEG Reaction of^ an Intact

and an Isolated Rabbit Brain During the

Influence of an SHF Field

/93

We already know that the reaction stability increases as the SHF field
strength is increased, after injection of caffeine, after mechanical damage to
the cortex (isolation of a strip), and after sectioning at the midbrain level.

A B CD E F

Figure 33. Dynamics of
the Appearance of EEG
Reactions to a 50 mw/cm
SHF Field on Separate
Rabbits. 1 = Presence of
the Reaction; 2 = Absence
of the Reaction; A-F =
Different Rabbits.

We decided to find out whether the effect of these
influences is additive, like the injection of caf-
feine and sectioning at the midbrain level, during

the influence of an SHF field of nonthermal intens-

2
ity (10 mw/cm ) .

These experiments were conducted on 25 animals.
The first group consisted of normal rabbits, the
second group was injected with caffeine, the third
group was sectioned at the midbrain level and, fin-
ally, the fourth group contained rabbits that were
injected with caffeine after the midbrain had been
sectioned. The method of recording the EEG and its
treatment was the same described above. Caffeine
sodium benzoate was injected intramuscularly in the
left thigh, 50 mg/kg, which relates to the doses
that caused an increase in the excitability of the
higher levels [Kalinlna, Tsobkallo, 1962]. Our
control tests on 3 rabbits showed that this dose of
caffeine reduces the amplitude and increases the
frequency of cortical potentials, but it does not
cause convulsive discharges.

We have already described the character of the rabbit EEG changes during
total-body unilateral exposure to a pulsed SHF field. In this series of tests,
it was interesting to follow the quantitative relationship of the different
forms of the EEG reaction, which depended to a great degree on the initial func-
tional state of the animal. The general results of this series of experiments
are given in Table 12. /94

TABLE 12. STABILITY OF THE EEG REACTION TO AN SHF FIELD IN AN
INTACT AND AN ISOLATED RABBIT BRAIN AFTER INJECTION OF CAFFEINE.

Test conditions

Number

of
rabbits

Number
of ex-
posures

Number
of con-
vulsive
reactions

Stability
%

Number

of all

reactions

Stability
%

normal animal

norm + caffeine

isolated brain

+ caffeine

As Table 12 shows. In normal rabbits the reaction stability is 32%, after
injection of caffeine it increases to 50%, after sectioning at the midbrain it
increases to 62%, and under the combined effect of these two forms of interven-
tion, it reaches 86%. In other words, the sensitivity of rabbits to an SHF •
field increases significently after pharmacological and surgical Intervention
in the activity of the CNS. However, sectioning at the midbrain increased the
sensitivity to a greater degree (approximately 2 times) than injection of caf-
feine (approximately 1.5 times), and the combined effect of these factors in-
creased the sensitivity by more than 2.5 times.

It is Interesting to note that the increase in reaction stability in the
last series of tests occurred only through an increase in the number of convul-
sive reactions, while the number of other forms of reaction was the same as in
the norm (29 and 28% respectively). In the second and third series, the in-
crease in reaction stability occurred due to both convulsive reactions and other
forms of EEG changes. Thus, the increase in the sensitivity of rabbits to an
SHF field following our interventions is manifested not only as an increase in
the number of EEG reactions during the influence of the field, but also as an
increase in the sharper, convulsive forms of the reaction.

5 1i 2i 35 V 55 S.' ?5 bt 95 C' .'5 '25 135 if5 155 155 m
fv.\ Be/Juyuha /iame»m,HOco nepuoM cex

Figure 34. Distribution of the Latent Periods of the EEG
Reactions to a Pulsed SHF Field in Normal Rabbits (1) , After
Injection of Caffeine (2), in an Isolated Brain (3) and in an
Isolated Brain After Injection of Caffeine (4). Key: (a)
Number of Changes, %; (b) Length of the Latent Period, Sec.

As we go to an analysis of the latent periods of the reaction, we should /95
note that it is difficult to characterize this index by an average magnitude
since the distribution of the values of the latent period is not a normal dis-
tribution. However, as Figure 34 shows, the shortest periods are observed after
the combined effect of sectioning and caffeine, then come the latent periods of
the reactions that occur after sectioning, behind them the latent periods of the
reactions after injection of caffeine and, finally, the reactions of normal rab-
bits have the longest latent periods. It is Important to emphasize that the

shortest latent periods of the reactions are very rarely less than 15 sec. Con-
sequently, even under the best conditions, EEG reactions to an SHP field retain
the properties that distinguish an SHI" field from other stimuli that affect spe-
cialized receptors. Thus, the stability, form and latent period of the EEG re-
action of rabbits, consistently attest that the sensitivity of the animal to an
SHE field increased after injection of caffeine, sectioning at the midbrain,
and the combined effect of both these factors.

We will not analyze the EEG reactions that occur at the moments the gener-
ator is turned on and off since these changes were insignificant in the differ-
ent series of tests. We decided to illustrate the change in the intensity and
duration of the basic reaction with a graph in which the results of the first
and last series of tests are compared, Figure 35 shows the dynamics of a num- /96
ber of slow and sharp waves that occur in the rabbit EEG. The changes were cal-
culated for each 10-second interval of the recording. The figure shows the
total picture obtained as a result of applying 50 exposures to a normal rabbit
(1) and 50 exposures to an isolated brain preparation after injection of caf-
feine (2). In this calculation, each slow and sharp wave was given a value of
one, and if convulsive discharges occupied the entire 10-second interval, they
were given a weight of 5. In other words, the presence of a change in a 2-sec-
ond recording of the EEG was taken as unity. Considering the great arbitrari-
ness of this quantitative evaluation of the EEG, we still decided to conduct it
because we were interested in a relative comparison of the background with the
influence on a normal rabbit, and on an isolated brain preparation after injec-
tion of caffeine.

(b) Bpei^'i . ceif

Figure 35. Djmamics of Slow and Sharp Waves in
the Electrical Brain Activity of a Normal Rabbit
(1) and in an Isolated Brain Preparation After
Injection of Caffeine (2) in the Background and
Under the Influence of a Pulsed SHF Field. The
Arrows Indicate the Moments the Generator was
Turned On and Off. A = Before Exposure; B = Dur-
ing Exposure; C = During the Aftereffect. Key:
(a) Number of Changes; (b) Time, Sec.

From the graph, it is
clear that the number of
slow and sharp waves in the
EEG of a normal rabbit be-
fore the start of the expos-
ure varied around 10, but
after 45 sec of exposure,
it had increased by 3.5
times, which is a statistic-
ally reliable change (p <
0.001). However, in spite
of the fact that exposure
continued, the number of
waves had a tendency to de-
crease, and before the gen-
erator was turned off, they
exceeded the background num-
ber by only 2.5 times.

Some 15-25 sec after
turn-off, we saw a new rise
in the number of slow and
sharp waves, which charac-
terizes the previously de-

scribed reaction to turn-off [Kholodov, 1962aJ .

In the isolated brain preparation, after injection of caffeine, the chang-
es in cortical biopotentials during exposure were revealed more clearly than in
the normal rabbit. The difference was that the increase in the changes in the
EEG occurred earlier and more sharply than in normal rabbits. In the back-
ground, the number of changes in a 10-second interval averaged 15, 35 sec after
the start of exposure this number had increased by 5 times, and after 95 sec,
by almost 6 times. Some 15-25 sec after exposure ended, as in the normal rab-
bit, we noted the reaction to turn-off. However, while the number of slow and
and sharp waves began to approach the background in normal rabbits after the ex-
posure ended, in the isolated brain preparation after injection of caffeine, the
number of changes exceeded the background by almost 4 times just one minute af-
ter the exposure ended. Thus, the increase in sensitivity to an SHF field after
operative and pharmacological intervention was also manifested as an increase in
the intensity of the basic reaction and as an increase in the aftereffect reac-
tion.

The fact that the eff€:cts of caffeine and sectioning are additive, i.e.,
during their combined effect the stability of the EEG reaction to an SHF field
increases to a greater degree than during the effect of one factor, forces us /97
to assume different mechanisms to explain the increase in sensitivity. As was
sho\m previously, by themselves these factors cause different changes in the
rabbit EEG. Caffeine quickens the biopotential oscillations and reduces the am-
plitude, and sectioning at the midbrain level leads to the appearance of slow
high-amplitude oscillations. Caffeine probably increases the sensitivity of
elements that react to an SHF field, and sectioning reduces the inhibition of
afferent effects that possibly interfere with the occurrence of a reaction to
the SHF field.

Discussion

We can increase the stability of a reaction to a UHF field by increasing
the power flux density of the field, or the sensitivity of the CNS, but even at
the optimal limit of the reaction, its stability does not exceed 90%. If we do
not disregard the remaining 10%, we should acknowledge that the statistical
character of the reaction is retained in all our experiments, as is its pro-
longed latent period. Consequently, these properties characterize the reaction
of the CNS to an SHF field of any of the strengths we used. It is difficult to
explain these properties of the reaction without knowing the essential biophysi-
cal mechanisms of the effect of a field on the cell. However, the thermal ef-
fect, which we tried to avoid by using a field of nonthermal intensity, still
was not fully precluded by these tests. The supporters of only the thermal ef-
fect of an SHF field can explain the obtained facts as selective heating of sep-
arate elements of the CNS. The statistical character of the reaction and its
prolonged latent period can also be explained by heating. We saw the final an-
swer to the question concerning the specific effect of EMF in the use of con-
stant magnetic and electric fields, which fully excluded the thermal effect.
The next chapter is devoted to these questions.

The form of the bioelectric reaction of the brain to SHF fields of different
82

wavelengths, different power flux densities and different character (constant,
interrupted and pulsed) was identical and similar to the form of the bioelectric
reaction to a UHF field. Other investigators [Gvozdikova et al., 1964a, b;
Zenina, 1964] have noted polymorphism of the EEG reactions of rabbits to an SHF
field, but changes towards predominance of slow waves occurred more frequently.
This fact testifies to the existence of a nonspecific electrical reaction of the
brain to radio-frequency EMF. Being a rather sensitive method for determining
the presence of an effect of EMF, electrography of the brain did not give us un-
equivocal testimony on the predominance of one or another nervous process. At /98
the different power flux densities, the external picture of the EEG reaction was
similar, but from the reactance curves, the changes in excitability of the cor-
tical terminations of the visual analysor were directed differently. It is pos-
sible that the nonspecificity of the EEG changes is explained by the absence of
differences in the effect of pulsed and constant fields, although certain auth-
ors [Abrikosov, 1958], using different methods of investigation, have observed
diametrically opposed effects of constant and pulsed UHF fields on the CNS.

By recording the electrical activity in an isolated brain preparation and
in a neuronally- isolated cortical strip, we were able to prove the existence of
a direct reaction of the brain to an SHF field. The isolated brain sections re-
acted to an SHF field, as to a UHF field, more rapidly than an intact brain.

The depth of penetration of microwave energy into the tissue of the manmial-
ian head at a frequency of 2,400 MHz is about 1 cm [Presman, 1963], which en-
sures a direct effect of EMF not only on the cortex, but also on the subcortical
formations of the brain. Since the depth of penetration is increased at a fre-
quency of 600 MHz, we should conclude that in our experiments Involving SHF
fields, the conditions for the direct effect of the field on the brain tissue
did exist.

The final integrated reaction of an organism, the electrical brain activi-
ty, is determined by both reflex and direct effects of environmental factors.
Judging from the fact that only reactions in the form of an increase in the num-
ber of slow waves and spindles occurred during the influence of strong SHF
fields on the head of a rabbit, we can essentially speak about a central effect
of an SHF field in our experimental conditions. Let us recall that an EEG re-
action, of reflex origin, to a UHF field was expressed in the occurrence of de-
synchronization.

The results of experiments described in this chapter support our observa-
tions concerning the presence of a reaction to EMF turn-off. The existence of
an off-effect is an important index of the functioning of many receptor systems
[Granit, 1957]. The long latent period (several seconds) of the reaction to
turn-off forces us to assume that some slow systems react to EMF. According to
this index, the basic reaction and the reaction to turn-off differ little from
each other. The latent period of the reaction to turn-off is somewhat smaller
than the latent period of the basic reaction. On the other hand, after section-
ing at the midbrain level and after isolation of a cortical strip, the stabili-
ty and the latent period of the basic reaction changed, but the corresponding
indices of the reaction to turn-off remained unchanged. These facts testify /99
to a certain independence of the analyzed reaction.

We should note that the aftereffect appeared not only during the off-effect,
hut also during the prolonged change of the electrical brain activity after the
SHF generator was turned off.

In studying the effect of an SHF field on the electrical activity of the
rabbit, we called attention to the brief desynchronization reactions that occur
at the moments the generator is turned on and off. These reactions were also
observed during the influence of a UHF field, but they were not analyzed in de-
tail. We consider these reactions to be orienting reactions that occur regard-
less of the basic character of the EEG reactions to EMF. A special analysis
showed that these orienting reactions are not connected with sound stimuli that
occur during operation of the generator [Zenina, 1964].

The use of caffeine showed that pharmacological analysis expands the possi-
bilities of investigating the mechanism of the effect of an SHF field on the CNS.
The appearance of convulsive discharges in the cortex after its excitability was
increased by caffeine shows that an SHF field can cause significant changes m
the activity of the CNS. From this point of view, the report [Zenina, 1964]
that prolonged exposure (1-2 months) to decimeter and centimeter waves can create
epileptoid readiness in the CNS, which is realized during sensory and electro-
magnetic provocation in the form of epileptoid bioelectric activity and some-
times in the form of convulsions is of interest.

In a study on the sensitivity of the CNS to SHF fields of different ranges
and different power flux densities, it was established that the sensitivity is
increased as the wavelength is increased from the centimeter to the meter range
and as the power flux density is increased in all ranges. The curve of CNS
sensitivity to an SHF field is close in form to the classical Weiss-Lapique
curve for electrical stimulation [Gvozdikova et al., 1964a, 1964b]. We cannot
now name the threshold intensity of an SHF field, although it is below 0.02 mw/
2

We must mention that EEG investigations on the effect of an SHF field on
the CNS have only begun, and we can shortly expect the appearance of many inter-
esting works in this area.

Conclusions

/lOO

1. The effect of a constant SHF field (wavelength, 12 cm) with power flux

densities from 100 to 1,000 mw/cm^ or a pulsed SHF field (wavelength, 52 cm)

with power flux densities from 2 to 50 mw/cm on the rabbit caused an increase
in the number of slow high-amplitude and spindle-shaped oscillations of cortical
biopotentials. Sometimes the SHF field caused the appearance of convulsive dis
charges in the cortex. The stability and the average latent period of the basic
reaction depended on the power flux density of the field. As the power flux
density was increased, the stability changed from 20 to 90%, and the average ^
latent period changed from 70 to 25 sec. The reaction to a pulsed ^^^^^^ J'^^^
spired with a shorter latent period than the reaction to a constant SHF field.

The excitability of the cortical termination of the visual analysor in-
creased during exposure to an SHE field with a power flux density of 100-300 mw/
2
cm and decreased during exposure to an SHI" field with a power flux density of

about 1,000 mw/cm , at which time the phase effect of the field appeared.

2. Besides the basic EEG reaction to an SHF field, we noted a reaction to
turn-off (the off-effect) with a stability 13-20% and an average latent period
of 15-20 sec. Furthermore, at the moment of turn-on (in 31% of the cases) and
at the moment of turn-off (in 12% of the cases) of the generator, brief desyn-
chronization occurred in the electrical brain activity, which reflected an ori-
enting reaction of the animal to the stimulus.

3. The electrical reaction of the brain to an SHF field was improved af-
ter sectioning at the level of the midbrain. The electrical activity of a neu-
ronally-isolated cortical strip changed during exposure more rapidly than the
EEG of an intact brain.

4. Intramuscular injection of caffeine shortened the latent period of the
EEG reaction to an SHF field, increased its stability and increased the number
of cases when convulsive activity appeared under the influence of the field.

5. Like a UHF field, an SHF field caused electrical reactions in the rab-
bit brain that transpired with a long latent period and a prolonged aftereffect
and that essentially occurred due to the direct effect of the field on the brain
tissue.

CHAPTER 3. THE EFFECT OF A CONSTANT MAGNETIC FIELD ON THE /lOl

ELECTRICAL ACTIVITY OF THE RABBIT BRAIN

While we referred our study of effects of UHF and SHF fields on the elec-
trical activity of the rabbit brain to the widely acknowledged existence of a
biological effect due to electromagnetic oscillations in these ranges, when we
go to an investigation on the effect of a constant magnetic field (CMF) , we
first of all discuss the question concerning the presence of a biological effect
due to this physical factor. The fact is that many authoritative investigators
deny the possibility of an effect of a magnetic field on biological processes.
The following statements can be given as an illustration.

1901. "Life has not developed special organs to perceive magnetic stimula-
tion because the magnetic effect does not exist as a stimulus for protoplasm"
[Danilewsky] .

1928. "We must admit that until now no bases for acknowledging the effect
of a constant magnetic field have been obtained" [Rozenberg] .**

1936. "Not enough is currently known regarding the effect of pure magne- ^^
tism. The organism does not have a sensitivity to magnetic fields" [Zeyfrits].

1948. "Magnetic fields are not perceived by an organism and, therefore,
they cannot play the role of stimuli" [Nasonov] .***

1961. "Thus, to the question of whether a constant magnetic field affects
living matter, we must presently answer no" [Blyumenfel'd] .t

1964. "Evidently, there is no sensory or metabolic reaction to a magnetic
field" [Akkerman] .tt

However, together with these statements, more and more experimental works
have appeared that have proved the presence of a biological effect due to mag-
netic fields. We shall try to briefly and chronologically examine the course of
the study on the effect of magnetic fields on biological objects.

*Danilewsky, V. J.: Issledovaniye nad fiziologicheskim deystviyem elek-
trichestva na rasstoyanii. (Investigation of the Long-Range Physiological
Effect of Electricity.) Volume 2, Kharkov, 1901, p. 9.

**Cited by V. I. Karmilov: The history of the question regarding the bio-
logical and therapeutic effect of a magnetic field. In the collection: Bio-
logicheskoye i lechebnoye deystviye magnitnogo polya i strogo-periodicheskoy
vibratsii. (Biological and Therapeutic Effect of a Magnetic Field and Strictly
Periodic Vibrations.) Perm, 1948, p. 9.

***Nasonov, D. N. : prirode vozbuzhdeniya. (On the Nature of Excitation.)
Moscow, 1948, p. 4. ,

tBlyumenfel'd, L. A.: On the problem of biomagnetism. Nauka i zhizn 7:
90, Moscow, 1961.

ttAkkerman, Yu. : Biofizika. (Biophysics.) Izd-vo "Mir", Moscow, 1964, p. :):j4.

The Biological Effect of Magnetic Fields /102

Before the XX century, many investigators had acknowledged the biological
effect of a magnetic field. We have taken the most detailed information on this
question from the book of N. I. Grigor'yev, "Metalloscopy and Metal Therapy,"
published in St. Peterburg in 1881.

In the XIX century, the study of the therapeutic effect of a magnetic field
was conducted on a scientific basis and was not surrounded by the mysticism that
distinguished similar investigations in preceding centuries.

Magglorani (1869) concluded that "If tests are conducted on healthy people,
on some a magnet will have its effect, and on others it will not. Hysteriacs,
those with ataxia, and diabetics are most sensitive to a magnet. The phenomena
caused by a magnet consist of a temperature rise, convulsions, tonic spasms,
anesthesia and hyperesthesia."*

A large series of works, which were begun by Charcot, were devoted to the
effect of a magnetic field on hysteriacs. It was noted that a magnet: 1)
causes itching, a creeping sensation, and twitching or pain at the exposure site
[Miiller, 1879; Drozdov, 1879]; 2) restores the destroyed sensitivity of the skin
[Westphal, 1878; Gamgee, 1878]* and of the retina [Charcot, Renard, 1878]* or
induces a "transfer" of anesthesia from the ill side of the body to the healthy
side [Jacond, 1880; Deboe, 1880]*; 3) destroys [Miiller, 1879]* or reduces paral-
ysis, spasms and contractures [Vlgouroux, 1878]*; 4) relieves pains of diverse
origin [Benedict, 1879*; Drozdov, 1879] or causes an increase in pain [Botkin,
1879]; 5) causes general weakness, headache and somnolence [Landouzy, 1879*;
Sprimon, 1879; Elenburg, 1911].

It can be seen that a brief listing of the various therapeutic effects of
a magnetic field indicates its predominant effect on the functions of the ner-
vous system. This property has also been noted by such authoritative physi-
cians as S. P. Botkin and J. M. Charcot.

However, the therapeutic properties of a magnetic field turned out to be
less effective in comparison with the new electrotherapy methods (d'arsonvall-
zation, diathermy, a UHF field, etc.).

In the XX century, we turn up only scattered reports on the therapeutic ef-
fect of a magnetic field; these works describe the favorable effect of a magne-
tic field on cancerous diseases [Spude, 1937; Barnothy, J., 1960; Ukolova,
Khimich, 1960], on radiation sickness [Barnothy, M. , 1963; Audo et al. , 1960],
for projected pain after amputation of the extremities, in clausalgia, nephritis / 10 3
and eczema [Karmllov, 1948], for Internal diseases [Seleznev, Bobrova, 1948a]
and cardiovascular diseases [Nemanova, 1948]. In recent years, there have been
reports of Investigations on magnetism for hypertensive disorders by Romanian
and Japanese doctors.

Unfortunately, the certain revival of Interest concerning the therapeutic

Cited by N. I. Grigor'yev: Metalloskopiya 1 metalloterapiya. (Metallo-
scopy and Metal Therapy.) St. Peterburg, 1881.

effect of a magnetic field Is not connected with any theoretical achievements
In this region. Until now, the mechanism of the effect of a magnetic field on
_ biological objecL remains unclear, which explains the predominantly empirical
direction of the works on the biological effect of a magnetic field.

In experiments on single cells, some investigators have noted a decrease
the intensity of movement, and a suppression of growth and multiplication
[Cheneveau, Bohn, 1903; Grenet, 1903; Kimball, 1938; Buksa, 1950; Platunova,
Korotkova, 1955; Gerencser et al. , 1962], and others have noted no biological
effect of a magnetic field [Luyet, 1935; Jennison, 1937].

In a series of works, P. F. Savostin (1928, 1937) showed that a magnetic
field changes protoplasmic streaming in plant cells (similar results were no-
ted by Ewart, 1903), increases the rate of root growth and cell membrane per-
meability. In tests on plants, it was observed that corn roots turned toward
the south pole of the magnet as they grew [Krylov, Tarakanova, 1960], watercress
roots were deflected toward the side with the least magnetic field strength
[Audus, 1960], and the seeds of many plants grew faster in a magnetic field
[Murphy, 1942], especially when they were oriented along the magnetic force
lines [Pittman, 1962, 1963].

In a magnetic field, the harvest of apples was increased [Karmilov, 1948]
and their ripening was accelerated [Boe, Salunkhe, 1963], photosynthesis in^
leaves was reduced [Tarchevskiy, 1964; Zabotin, Nazarova, 1964], and the orien-
tation of volvox changed [Palmer, 1963].

In the majority of works on the effect of a magnetic field on plants, it
has been stated that the observed effect is achieved through enzymatic processes.

In a culture of chick embryo heart tissue, placed in a magnetic field, a-
typical cells, sometimes multicellular and of giant dimensions [Lengyel, 1934],
cellular migration in the direction of the electromagnet poles [Huzella, 1934],
and sometimes only a weak tendency toward protoplasmic disintegration
[Payne-Scott, Love, 1936] have been observed. Cultures of tumorous cells re-
quired less oxygen in a magnetic field [Reno, Nutini, 1963], and after 18 hours
in the field, a large part of the cells had fully degenerated [Mulay and Mulay,
1961].

A culture of mouse embryo kidney tissue required 87% less oxygen in a mag-
netic field, although this effect was not revealed in tissue from adult mice /104
[Reno, Nutini, 1963].

In studies on the effect of a magnetic field on a frog neuromuscular prepa-
ration, some investigators have shown that this physical factor does not have a
stimulating effect, although its chronaxy exhibits a two-phase change, initially
increasing, and then decreasing, and parabiosis is removed [Petrov, 1930; Erdman,
1955]. Other investigators deny any effect of a magnetic field on a neuromus-
cular preparation [Liberman et al., 1959].

From approximately 1938, questions regarding the biological effect of a
magnetic field were intensively formulated in the Perm Medical Institute. In
vitro , it was noted that during the effect of a magnetic field: 1) according

to some authors [Kyuntsel' and Karmilov, 1947], the blood coagulation time in-
creases, but according to others [Tishan'kin, 1948] this rate decreases; 2) the
erythrocyte sedimentation rate slows down [Mogendovich, Sherstneva, 1947;
Mogendovich, Sherstneva, 1948a; Mogendovich, Tishan'kin, 1948a, b]; 3) sinking
of a drop of blood in a copper sulfate solution (the microgravitatlonal effect)
is accelerated [Mogendovich, Sherstneva, 1948b]; 4) leukocyte phagocytosis in-
creases [Sherstneva, 1950].

In her dissertation, 0. S. Sherstneva (1951) noted that a magnetic field
affects the phagocytes of an Intact organism primarily through the CNS. This
explains the greater sensitivity of the entire organism to a magnetic field in
comparison to Isolated organs and tissues.

It has been noted that the lower invertebrates, such as the planaria and
gastropods, can orient along an artificial magnetic field that exceeds the na-
tural magnetic field of the earth by only several times. It has been noted that
this orientation depends on the diurnal, lunar and solar cycles [Brown, 1962;
Prosser, Brown, 1961; and others]. In a magnetic field, the reproduction of
Daphne [Luczak, 1961] and Drosophlla [Llvengood, Shinke, 1962] is inhibited and
the mortality rate of exposed Drosophlla [Forssberg, 1940] Increases. Orienta-
tion of files and termites has been noted in both a natural and an artificial
magnetic field [Becker, G. , 1963a, b].

The possibility of an orienting effect of the earth's magnetic field has
been studied particularly intensely on such migrating fauna as birds and fish.

In 1885, the Russian Academician A. T. Mlddendorf, while studying the per-
iods of spring migration of certain Siberian birds, expressed the concept con-
cerning the possible orientation of birds along the earth's magnetic field.

The American physicist Yeagley (1947, 1951), experimentally proved that
pigeons orient with respect to the earth's magnetic field and the Coriolis forca/105
He trained pigeons to return to their loft from remote distances. Then the pi-
geons were transported thousands of kilometers away to a place where the earth's
magnetic field and Coriolis force were similar to what they were at the training
ground. When they were released in this unfamiliar locality, the pigeons were
able to find their loft.

The possibility of pigeons perceiving the earth's magnetic field was check-
ed in experiments in which magnets were tied to the wings of test pigeons, and
copper plates were tied to the wings of control pigeons. From an equal dis-
tance, the control pigeons returned to their loft sooner than the pigeons bear-
ing magnets.

However, numerous repetitions of Yeagley 's tests have not supported these
results [Gordon, 1948; Van Riper, Kalmbach, 1952; Griffin, 1955; and others].
Attempts to develop a conditioned reflex to a magnetic field in pigeons were
unsuccessful [Orgel, Smith, 1954, 1956; Neville, 1955; Kholodov, 1959]. How-
ever, in tests employing a maze, the weak effect of a magnetic field on the be-
havior of birds was noted [Neville, 1955], and in tests with the food-getting
method (these tests were similar to those described below), there was an Increase
in the intersignal reactions and Inhibition of the developed conditioned re-

flexes to light [Kholodov, 1959]. A magnetic field increased the motor activity
of certain sparrows [El'darov and Kholodov, 1964]. These positive results force
us to assume that the question of the effect of a magnetic field on the behavior
of birds requires further experimental clarification.

Experiments involving the development of conditioned reflexes to a magnetic
field in fish were more successful. Simultaneously and independently, Lissman
(1958) did this on the Nile electric fish (Mormlrus) , and Kholodov (1958b) on
carp. Furthermore, it was shown that a magnetic field can be a conditioned-
inhibiting stimulus in tests on stickleback, and can inhibit developed condi-
tioned reflexes to light and sound in bullhead and flounder [Kholodov, Verevkina,
1962]. The sensitivity of stickleback to a constant electrical current was re-
duced by 45% [Kholodov, Akhmedov, 1962] and their motor activity was increased
[Kholodov, 1959] during the influence of a magnetic field.

Thus, the hypothesis concerning the ecological significance of the earth's
magnetic field has directed investigators to study the behavior of animals under
the influence of this physical factor, and the important role of the CNS has
become clearer here than in investigations on radio-frequency EMF.

We have begun our description of the effect of UHF and SHF fields on the
CNS with the results of observations of people who work under conditions of /106
prolonged exposure to these factors.

In people subjected to the prolonged effect of magnetic fields (hands in a
350-3, 500-Oe field, but head in a field less than 150-250 Oe) for 20-60% of
their working time, deviations were most frequently noted in the nervous system
[Vyalov et al., 1964]. These deviations were characterized by headaches, pains
in the heart region, fatigability, a reduced and unstable appetite, insomnia,
increased sweating, and sensations of itching and burning on the hands.

When the EEGs of these people were investigated, a tendency was noted to-
ward predominance of the process of cerebral inhibition. Slow waves and spin-
dles of a-rhythm were noted during rest and during the light test,

Otoneurological investigations most frequently indicated the central origin
of suppression of this apparatus (paravestibular destruction). A tendency to-
ward bradycardia was noted during an investigation of the cardiovascular system.
The authors assume that magnetic fields most frequently cause the first stage of
parabiosis according to W. Ye. Wwendensky (a change in lability) in the autonom-
ic nervous system. Although it is concluded that the observed changes do not
exceed the physiological limits of change, one can see that the tendency toward
change of the organism's functional state is the same as during the influence
of physiologically stronger stimuli, such as UHF and SHF fields.

We should remember that the magnetic field also has a sensory effect on the
visual analysor. D'Arsonval (1893) first showed that the phenomenon of phosphene
occurs in man during the influence of a variable magnetic field. This fact was
later noticed in many works [Danllewsky, 1905; Thompson, 1910; Mognisson, Steven,
1911; Barlow et al. , 1946; Mogendovich, Skachedub, 1957; and others]. Phosphene
can be produced during a constant magnetic field or in its absence, and also
during the influence of a variable magnetic field with a frequency of 10-100 Hz.

It is considered that the sensation of phosphene is a result of retinal stimu-
lation [Mogendovich, 1956; Mogendovich, Skachedub, 1957].

It was assumed that the phenomenon of phosphene occurs only due to an in-
duced electromotive force; however, in the work of N. A. Solov'yev (1963), it
was shown that the magnitude of phosphene depends on the exposure duration to
the magnetic field.

Besides phosphene, other changes were noted in the activity of the visual
analysor under the influence of a magnetic field. In a variable magnetic field,
the stability of clear vision was reduced in man [Mogendovich and Skachedub,
1957], A constant magnetic field applied to the occiput of the subject changed
the visual images that were suggested in hypnosis (Fere, 1885; Binet, Fere, /107
1887; Vasil'yev, 1921] and reinforced the visual hallucinations caused by mes-
caline intoxication [Perikhanyants , Terent'yev, 1947].

Although the important role of the nervous system in the reactions of ver-
tebrates to a magnetic field is emphasized in many works, the changes which oc-
cur are not limited to just this system.

During total-body exposure to a magnetic field, the Perm investigators have
noted an Increase in the number of leukocytes and a lowering of the resistance
of erythrocytes in guinea pigs [Karmilov, 1948], a 10% increase in the body
weight of mice in comparison with controls [Karmilov, 1948] and a reduction in
their oxygen requirement [Tishan'kin, 1950]. An increase in the acidity of the
gastric juice [Seleznev, Bobrova, 1948b] and an increase in peristalsis of the
large intestine [Suvorova, 1948] have been noted in people under the influence
of a magnetic field.

Investigations of the osmotic processes in muscle [Bekker, Mogendovich,
1948] and the permeability of skeletal muscle by means of staining [Skachedub,
1948] showed that a magnetic field increases the permeability. However, other
investigators [Troshina, 1951] do not support these results.

When guinea pigs were placed in a 700-Oe field 6 times for 30 min each,
there was dilation of the vessels with subsequent hyperemia and hemorrhage, es-
pecially in the lungs, liver and CNS [Karmilov, 1948].

The effect of a constant 2,000-5,000-Oe magnetic field on mice has been
studied most systematically by the American investigators, M. F. Barnothy and
J. M. Barnothy, in 1954-1958 [Barnothy, I960]. It was shown that pregnant fe-
males placed in a 2,500-Oe field gave birth to healthy offspring, but the young
were approximately 20% smaller than those born previously of the same mothers.
When a 3,100-Oe field acted on the pregnant females, the newborn lived only
several days, and under a 4,200-Oe field the embryos were resorbed in the uterus.
Young that were placed in a field at 3-4 weeks of age grew more slowly than con-
trol animals, and the males grew slower than the females. A strong field (5,000
Oe) greatly delayed growth. The magnetic field had practically no effect on the
weight of adult mice.

If mice were placed in a field before they attained full growth, they could
adapt to and develop in the magnetic field, but adult, 7-week-old males died af-

ter 10 days in a field. However, not one female died during these experiments.

In the males that died in the field, the weight of the liver was 50% lower
than in the control animals, although the weight of the lungs, heart, kidneys
and testicles did not change. In mice that had been subjected to a 4,200-Oe
field for 5 weeks, and killed 3 months after the end of the experiments, neo- /108
blasts were found in the spleen.

The rectal temperature of mice was lowered 0.8°C in a magnetic field. This
lowering of temperature was still retained months after exposure had ended.
Following an exposure to a field, the motor activity of mice was increased by
50%. Animals that had been subjected to a field ate approximately 14% less food
than the controls, and if we calculate the metabolic efficiency (food/motor ac-
tivity) , in the test animals it was 60% higher.

The fur of adult animals that had been in a magnetic field from youth was
fully retained, while in control animals of the same age (1 year) full balding
of the abdomen had occurred.

The menstrual cycle of females subjected to a field was disturbed, but it
was immediately restored after the influence ceased. Impregnation was not ob-
served when males and females were placed together in a 3,000-Oe field, but it
could occur if one of the partners was not in the field.

The RBC did not change in the field, but the WBC was reduced by 30-40%.^
After the influence ceased, the WBC increased to 100% of the initial level, in
the next two weeks it was somewhat below the initial level, and after months it
had returned to normal. The number of lymphocytes increased only following the
field influence.

The changes observed in the blood composition allow us to use a magnetic
field as a means of preventing the development of radiation sickness, during^
which the WBC is sharply reduced. While the effect of radiation led to a 30%
death rate, a preliminary stay in a magnetic field completely eliminated the
lethal effect. If, however, radiation caused death in 80%, the death rate was
not reduced, but mice that had stayed in a magnetic field died later than the
controls.

After inoculation with breast tumor, mice that had stayed in a magnetic
field lived 43 ± 9% longer than the controls, but the weight of the tumor was
250% higher than in the control period. Autopsy revealed that the magnetic
field prevents metastasis, but does not obstruct the growth of a primary tumor.

The effect of a magnetic field on development, blood picture or tumors was
not observed by one author [Eiselein, et al. , 1961]. However, works that sup-
port the Barnothy conclusions are more numerous. A decrease in the weight (7
9%) and the WBC in guinea pigs following a 24-hour exposure to a 200-Oe field
with a frequency of 50 Hz has been noted by investigators from the Tomsk Medi
cal Institute [Kurlov et al., 1963]. During similar exposures, other Investi-
gators from the same institute [Gorshenina, 1963] observed that the shock or- ^iuy
gans," i.e., the organs in which the sharpest morphological changes are observed,
are the spleen, testicles, and also the CNS.

Determining the absorption of oxygen by kidney tissue, Reno and Nutini
(1963) noted a greater susceptibility to damage in embryonal tissue during the
influence of a magnetic field. An increase in motor activity in a magnetic
field was noted by T. I. Gorshenina in guinea pigs, and also by Yu. A. Kholodov
(1959) in fish and birds.

Thus, we can see with each passing year, the number of reports indicating
the existence of a biological effect of a magnetic field has increased.

The largest group of investigators working on this problem in our country
is concentrated in the Tomsk Medical Institute. Since 1959, under the leader-
ship of Corresponding Member of the USSR Academy of Medical Sciences I. V.
Toroptsev, they have studied the morphological changes occurring in animals in
a magnetic field. After 500 hours (at night, 12-hour sessions) in a constant
7,000-Oe field, necrotic processes connected with metabolic disturbances occur-
red in guinea pigs. The testicles were damaged most, then the spleen and, fin-
ally, the lungs. Disturbances in the hemodynamics and lymphodynamics were re-
vealed with sufficient clarity [Toroptsev, Garganeyev, 1964b]. A variable (50-
Hz) magnetic field with a strength of 200 Oe has a similar biological effect
[Toroptsev, Garganeyev, 1964a]; during a single 7-hour exposure, this field not
only caused morphological changes in the lungs (hemorrhage, edema), but it also
disturbed the chemical composition of the stroma [Gorshenina, 1964].

Morphological changes in the spinal cord and skeletal musculature of mice
under the effect of a magnetic field were particularly clearly revealed during
functional loading in the form of swimming for 15 min [Rassadin, 1964]. A sin-
gle 6-hour exposure to a field did not bring about visible qualitative changes
in the morphological and histochemical aspects of immunogenesis [Vasil'yev et
al. , 1964], but multiple 6-hour exposures for 15 days lowered the natural resis-
tance of white mice to Listeria [Odintsov, 1964]. N. V. Vasil'yev (1964) showed
that a variable or a constant magnetic field affects immunogenesis, intensifying
it in some cases (the formation of hemagglutinin) and suppressing it in others
(the development of antiviral immunity).

The development of magnetobiology (or biomagnetism) can be illustrated by
the increase in the number of scientific conferences where questions on the
biological effect of magnetic fields are discussed. The First International /llO
Symposium on Biomagnetism was held in 1961 in Chicago [Barnothy, 1962]; 8 re-
ports were presented and discussed. The second such symposium was held in 1963,
and 17 reports were presented. The material from this symposiimi is reflected in
the collection, "Biological Effects of Magnetic Fields" edited by M. F. Barnothy,
which was published in 1964. This book significantly supplements the reports
concerning the biological effect of magnetic fields found in the Russian collec-
tion, "The Biological and Therapeutic Effect of a Magnetic Field and Strictly
Periodic Vibrations", which was published in 1948. In October, 1963, at the In-
stitute of Labor Hygiene and Occupational Diseases of the USSR Academy of Medi-
cal Sciences (Moscow), the first Soviet symposium on the biological effect of a
constant magnetic field and static electricity was held. In 1964, there was a
scientific conference of the Tomsk Medical Institute, which included a section
entitled "The Effect of Magnetic Fields on an Organism"; 14 reports were pre-
sented. The second such conference was held in June, 1965. The Third Interna-
tional Symposium on Biomagnetism was held in March, 1966, in Chicago (USA).

A bibliography of works on the biological effect of magnetic fields [Davis
et al., 1962], which reflects the interest of many scientific workers in the
evolution of this problem, has been published in the U.S.

A survey of the achievements of magnetobiology testifies to the complexity
of the problem and to the significant successes in the area of proving the exis-
tence of the biological effect of a magnetic field.

The effect of a magnetic field on electrical brain activity has been stud-
ied by us [Kholodov, 1963a, b; Kholodov, Luk'yanova, 1964] and by Romanian in-
vestigators [Dinculescu et al., 1963], who revealed an Increase in the A-rhythm
in man during the influence of a weak constant magnetic field. The American
biologist, R. Becker (1963), reported on the appearance of slow high-amplitude
oscillations in the electrical brain activity of a salamander during the influ-
ence of a constant magnetic field with a strength of several thousand Oe.

The Effect of a Constant Magnetic Field on the Rabbit EEG

Before we speak about the presence or absence of an electrical reaction in
the rabbit brain to a CMF, we should find out what indices must be evaluated to
reflect such an effect. The study of the rabbit EEG reaction to UHF and SHE
fields has shown that changes are most frequently manifested as an increase in
the number of spindles and slow waves. However, we have not conducted a detail-
ed analysis of the other EEG changes under the effect of these EMF.

Since the biological effect of a CMF is considered to be weaker, we con- /111
ducted a stricter treatment of the EEG in experiments involving this physical
factor. Besides spindles (or EEG changes resembling spindles) and slow waves
(biopotential oscillations with a frequency less than 4 Hz and an amplitude at
least twice the background amplitude), S. N. Luk'yanova, in experiments on 13
rabbits, also considered the dynamics of cycles, sharp waves and sections of the
recording of electrical activity with reduced amplitude or with increased ampli-
tude without frequency changes and with desynchronization, i.e., decrease in am-
plitude and increase in frequency of the potentials in the background and during
a 1-minute exposure to a CMF.

The data were statistically treated by the previously given method, and the
results of evaluating the difference (in the corresponding indices of electrical
activity in certain brain sections) between the 1-minute recording of the back-
ground and during exposure to 400-Oe CMF are given in Table 13. The table gives
the total result of 1,270 exposures on 13 normal rabbits, utilizing the Student
criterion. Values of this criterion that exceeded 1.9 (underlined in the table)
were considered reliable (p < 0.05). During the influence of the CMF, a number
of characteristics of the electrical activity frequently increased. The numbers / 112
noted with asterisks testify to a decrease in the corresponding changes of elec-
trical activity under the influence of a CMF. A dash indicates that changes
were not observed in the given lead.

The tabular data show that the most frequent and reliable result of expo-
sure to a CMF is an increase in the number of spindles, slow waves and sometimes
sharp waves. The other indices of electrical brain activity did not reveal sig-/113
nificant changes during the influence of a CMF.
94

TABLE 13. THE VALUE OF THE STUDENT CRITERION DURING A COMPARI-
SON OF A NUMBER OF DIFFERENT INDICES OF ELECTRICAL ACTIVITY IN
CERTAIN SECTIONS OF THE RABBIT BRAIN, FOR 1-MINUTE RECORDINGS
OF THE BACKGROUND, AND EXPOSURE TO A CMF ON THE ANIMAL'S HEAD.

1 >i

O 0)

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O JH

Character of the

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tl) O C!

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0.03

2.7

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sharp waves

3.7

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peaks

0.5

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1.0

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increased amplitude .

1.0

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0.8*

decreased amplitude .

1.8

1.3

0.1

0.8

0.2

1.6

0.5

desynchronization . .

0.1

0.5

0.06*

0.5

Note: the underlined numbers in the table are explained in the text.

Thus, as during the influence of UHF and SHF fields on the rabbit head, a
CMF affects electrical brain activity, increasing the number of spindles and
slow waves. In the further analysis of the rabbit EEC reaction to a CMF, we
shall be limited to a consideration of only these indices.

The table also shows that the most intensive reaction is observed in the
hypothalamus. However, we consider it more expedient to discuss below the ques-
tion concerning the participation of different sections of the brain in the re-
action to a CMF. At this stage of the analysis, it is sufficient to know that
the EEG of the sensorimotor and visual regions of the rabbit cortex change un-
der the influence of an 800-1, 000-Oe CMF, and that from the results for 100 ex-
posures on 12 rabbits, this change is manifested as an Increase in the number
of spindles (30% of the cases) and slow waves (19% of the cases), and sometimes
(4% of the cases) in the appearance of sharp waves (Figure 36).

After establishing the character of the rabbit EEG reaction to a CMF, it
was rather easy to determine the dynamics of the EEG changes that made up the
reaction, the reaction stability and the latent period, and also to analyze the
aftereffect, i.e., to repeat the analysis that we used during the influence of
UHF and SHF fields on the rabbit head.

Figure 37 gives the results of counting the number of changes, i.e., the
number of spindles and slow waves, for each 10 seconds of the EEG recording.
This graph is composed from the results of 100 3-minute exposures to a 1, 000-Oe
CMF on 12 rabbits. Although we saw sharp, at least 30 second long, changes in
the EEG with a definite latent period in only 53% of the cases (stability),
adding up of all the influences shows that when the electromagnet is turned on,

f 7
'I

B 2

.-»;,'V4»~

1 B

(b) ICCK

Figure 36. Forms of the EEG Changes During the Influ-
ence of a CMF on an Intact Rabbit. I = Increased Num-
ber of Spindles; II = Increased Number of Slow Waves;
III = Increased Number of Sharp Waves; A = Before Ex-
posure; B = During Exposure; 1 = EEG of the Sensorimo-
tor Cortex; 2 = EEG of the Visual Cortex. Key: (a)
200 yv; (b) 1 Sec.

we observe a significant (by a factor of 2) and statistically reliable (p <
0.001) increase in the number of changes. We must call attention to the fact
that in the background recording the number of changes was approximately iden-
tical in each 10-second interval. The significant rise in the curve begins at
the 15th second of exposure, but, having achieved a maximum at the 45th second,
the number of spindles and slow waves begins to decrease, regardless of the con-/114
tinning influence of the magnetic field.

It is interesting that immediately after the electromagnet is turned off,
the number of changes decreases. At the moment the electromagnet is turned off,
as when it is turned on, we sometimes observe a brief desynchronization reaction
in the EEG. However, 15-25 seconds after turn-off there is a statistically re-

.^ in
\ ,w

5 in

•5.7/7

(a) '"

5i3!i li

{h)'P'

.! 25 a

Figure 37. Dynamics of Slow
Waves and Spindles in the
Electrical Brain Activity of
a Normal Rabbit, in the Back-
ground and During Exposure to
a 1,000-Oe CMF. A = Before
Exposure; B = During Exposure;
C = During the Aftereffect.
Key: (a) Number of Changes;
(b) Time, Sec.

liable (p < 0.001) increase in the number of
spindles and slow waves, which almost approxi-
mates the basic reaction in intensity, but
lags behind it in duration. This reaction to
turn-off (Figure 38) sometimes occurs in the
absence of the basic reaction.

The above results of statistical treat-
ment allow us to assert with a high- degree of
reliability that a CMF of the applied strength
has an effect on the functional state of the
CNS; it increases the number of spindles and
slow high-amplitude oscillations. Some physi-
ologists connect these changes in the EEC with
the appearance of inhibition. We decided to
determine the directivity of the nervous pro-
cess that occurs under the influence of a mag-
netic field by using M. N. Livanov's method
of reactance curves.

We recorded the reactance curve 90 times
from 3 rabbits. The time for the appearance
of the assimilation reaction to light flashes of increasing brightness was 18 ±
0.4 sec in the background, 20.4 ± 0.4 sec under the influence of a 200-Oe magne-
tic field, and 20.0 ± 0.5 sec 30 seconds after the electromagnet was turned off.
The difference between these figures, as evaluated employing the Student cri-
terion, is statistically reliable (p < 0.001). Consequently, a magnetic field
causes a reduction in excitability of the cortical terminations of the visual
analysor and this effect continues for some time after the electromagnet is
turned off.

We obtained similar results during the influence of a strong SHF field, in
which case this effect could be explained by heating. The present experiments
testify to the possibility of an inhibiting effect of EMF without heating.

In determining the latent period of the reaction, we usually counted only
the stable EEC changes, i.e., the changes that lasted at least 30 seconds. Such
changes occurred in 53% of the cases, which characterizes the reaction stability.
However, in comparing the distribution of the latent periods of stable and un-
stable (changes lasting less than 30 sec) reactions, we found that the curves
repeat themselves. This fact points out that the formal separation of stable
changes does not fully characterize the EEC reaction to a CMF. Nevertheless,
since we distinguished only the prolonged EEC changes as reactions during ex- /116
posure to UHF and SHF fields, we will most frequently consider only the stable
reactions in the future.

We see that the latent period distribution curve of the EEC reactions to a
CMF has three peaks (see Figure 39). The first peak occurs at the 25th second
of the influence, the second, at the 55th second, and the third, at the 115th
second. The presence of several peaks testifies to the periodicity of the re-
action or to the participation of several mechanisms in its effectuation. The
small size of the third peak allows us to disregard it in subsequent investiga-

tions when the time of exposure
to the CMF was shortened to 1 :
min. The EEG reaction to a
CMF has a long latent period,
measured in seconds, and in
this respect it is similar to
the EEG reaction during the in-
fluence of UHF and SHE fields.

While the effects obtain-
ed under the influence of UHF
and SHE fields can be explained
to some degree by heating, the
effects obtained under the in-
fluence of a CMF could be ex-
plained by simple induction of
an electrical current. If an
electromotive induction force
plays the main role in the EEG
reactions under the influence
of a CMF, then we should see
the greatest change on the EEG
at the moment the electromagnet
is turned on or off, when the
induced electromotive force has
its greatest magnitude; in ac-
tuality, however, at the moments /117
the electromagnet is turned on
and off we sometimes observed
only an unpro longed (2-10 sec)
desynchronization reaction
(Figure 40) . For 175 exposures
in 15 rabbits, the stability of
the desynchronization at turn-
on is calculated as 14%, and
the stability of the same reac-
tion at turn-off, 24%. The
more frequent reactions at turn-
off (in comparison with those
at turn-on) can testify that
these desynchronization reac-
tions are caused by the induced
electromotive force, which is

^ Experiments involving a slower turn-on and

turn-off of the electromagnet by means of a rheostat led us to the same conclu-
sion. In this case, it is significant that reactions during turn-on and turn-
off are encountered more rarely. In a series of tests conducted by R. A.
Chizhenkova, in which an electromagnet was turned on for 2-3 sec, only the de-
synchronization reaction was observed.

These experimental results show that the induced electromotive force which
occurs when an electromagnet is turned on and off does not play a significant

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greater at turn-off than at turn-on.

¥5 65 85 /.OS 125 5

(b) 0ejiuvuMa mmi:nmNoso nepuoda, cen.

Figure 39. Latent Period Distribution Curves of the
Basic Electrical Reactions (A) and the Reactions to
Turn-Of f (B) of a CMF and an Intact Brain (1) , in an
Isolated Brain Preparation (2) and in a Neuronally-
Isolated Cortical Strip (3). Key: (a) Number of Cases,
%; (b) Length of the Latent Period, Sec.

role in generating the basic EEC reaction to a CMF. The basic reaction occurs
more frequently, has a longer latent period and a different electrographic mani-
festation than the EEC reaction that occurs at turn-on and turn-off.

icW?v»\,'*v,'vv'-v'*H\

.'■"• "' ' % " 'f- . (a) iS5K/W^L_ 1

Figure 40. Changes in Cortical Biopotentials Occurring at
the Moment the Electromagnet is Turned On. 1 = Sensori-
motor Region; 2 = Visual Region; the Arrow Indicates the
Moment the Electromagnet is Turned On. Key: (a) 200 uv;
(b) 1 Sec.

The possibility that the effect of a CMF is effected by the mechanism of an
electromotive force induced in the moving elements of the organism is not pre-
cluded. The effect of a CMF on the blood flowing through the vessels is especi-
ally probable. In the case of the effect of a CMF on the hind legs of a rabbit,
we did not observe changes in the EEC, while the effect of a weaker CMF on the
head of the same animal caused the usual EEG reaction. Of course, these experi-
ments do not answer the question concerning the participation of an induced

electromotive force in the observed reactions to a CMF, but they definitely tes-
tify to the high sensitivity of the rabbit head to a CMF in comparison with oth-

ay a a r> i- n r\rt^cr Qf itS ^-'^-^'

Finishing our analysis of the EEG reactions occurring at turn-on and turn- /118n
off, we must note that the observed phenomena can possibly occur with no connec-
tion to the physiological process of an induced electromotive force, but are an
expression of a negative physiological reaction that occurs at the beginning and
after the end of the influence of many stimuli [Sokolov, 1958]. However, a fin-
al judgment regarding the mechanism of the described reactions will become pos-
sible after additional experimental material has been accumulated.

We have already spoken of the reaction to turn-off when we discussed the
EEG reaction to UHF and SHF fields, and also when we described the dynamics of
the EEG changes under the influence of a CMF. This reaction was electrographi-
cally similar to the basic reaction, i.e., it was manifested as an increase in
the number of spindles and slow waves. In the distribution of the latent peri-
ods of the reaction to turn-off, the mode appeared at 15 sec (see Figure 39, B) .
Thus, the reaction to turn-off has a shorter latent period than the basic reac-
tion.

We should note that in evaluating the reactions to turn-off, we basically
considered unstable changes, i.e., EEG changes that lasted less than 30 sec.
Therefore, in comparing the stability of the basic reaction and the reaction to
turn-off, we should consider all changes in both cases. While the stability of
the basic reaction in such an evaluation reached 95%, the stability of the re-
action to turn-off attained 58%, i.e., it was 2/3rds as stable as the basic re-
action.

Thus, the reaction to turn-off is an independent reaction. It cannot be
considered a form of the reaction that occurs at some stage after the electro-
magnet is turned on regardless of whether we turn it off or not. The reaction
to turn-off occurs with a similar latent period after exposures of 30 sec, 1
min, 3 min and 15 min to a CMF. Since it is not observed after an exposure that
lasts 2-3 sec or 30 min, it depends on the duration of the CMF exposure. In our
experiments, the stopping of the exposure is the determinant for this reaction.

On the other hand, the reaction to turn-off is not connected with an in-
duced electromotive force since: 1) this reaction has a long latent period; 2)
it is retained during slow switching-on of the electromagnet by means of a rheo-
stat; 3) it is absent following exposure to a CMF that lasts 2-3 sec. It is
probable that some slow processes connected with the CMF exposure also slowly
normalize after the exposure has ended, and this fact finds expression in the
reaction to turn-off. The presence of this reaction proves the existence of a
biological effect due to a CMF, and indicates that it is difficult to identify
this effect with the effect of an electrical current.

The Effect of a CMF on the EEG of an Isolated Brain /119

To clarify the mechanism of the effect of a CMF, we conducted a series of
tests on an isolated rabbit brain preparation obtained after sectioning at the

100

level of the midbrain. Since the EEG reactions to a CMF in a brain preparation
did not change after additional bilateral sectioning of the olfactory and optic
nerves, we combined all the results obtained after sectioning at the midbrain
and after additional deafferentation into one group.

Sectioning was conducted on 11 rabbits, which were then exposed 209 times
to a CMF.

The character of the EEG reactions to a CMF after sectioning consisted of
an increase in the number of spindles and slow waves (40% of the cases) . Some-
times convulsive discharges occurred during the influence (12%) , or we observed
the disappearance of these discharges (9%) if they existed in the background
(Figure 41) .

% 4"- i •. '

\M: ? ^-

"•^v

•' ■ .^ /-t ...-■

't (a) 200mk6\

(b) ken :

Figure 41. Forms of the EEG Changes During the Influence
of a CMF on an Isolated Rabbit Brain Preparation. I = In-
creased Number of Sharp Waves; II = Increased Number of Slow
Waves and Spindles; III = Increased Number of Sharp Waves;
A = Before Exposure; B = During Exposure; 1 = EEG of the
Sensorimotor Cortex; 2 = EEG of the Visual Cortex. Key:
(a) 200 yv; (b) 1 Sec.

Here the reaction stability is evaluated from the stable EEG changes. Af-
ter sectioning, we observed a tendency towards an increase in the stability of
stable reactions to a CMF from 52 ± 5% to 61 ± 5%, which, however, is not a sta-
tistically reliable increase. The stability of unstable reactions did not change
after sectioning, remaining equal to 95%.

101

P i^a.) Ao nepepcjhu

ffZ3^2V^27

■ /
U?.

(b)„

fti

,Va //v w.'u !::^-!V'^-'5

uSulOf^-Zi'^^O

■

Figure 42. Dynamics of the Appearance
of EEG Reactions in Rabbits During the
Influence of a CMF Before and After Sec-
tioning at the Midbrain. In the Control
Experiments, We Administered "False" Ex-
posures. A = Test Animals; B = Control
Animals; the Numbers Indicate the Number
of the Rabbit; 1 = Presence of the Reac-
tion; 2 = Absence of the Reaction. Key:
(a) Before Sectioning; (b) After Sec-
tioning.

The number of stable reactions
to a CMF and similar changes in the
rabbit EEG during "false" exposures
for a normal and an isolated brain
is ahown in Figure 42. Each column
on the figure includes the number
of exposures for one rabbit. A
crosshatched square designates the
presence of a reaction, and a blank
square, its absence. Except for
rabbits no. 1 and 4, we investiga-
ted the reaction to a magnetic
field in the normal brain and af-
ter sectioning at the midbrain in
the same rabbits. As a comparison
shows, EEG changes similar to the
reaction were encountered in the
controls 5 times less often than
in the test with normal rabbits,
and 10 times less often than in an
isolated brain preparation. Thus,
the isolated brain reacted more
frequently to a CMF than a normal
brain.

Comparing the different col-
umns, we could not observe the pro-
cess of adaptation or summation in
the normal brain. In the isolated
brain we observed a tendency toward
an increase in the stability of the
reaction to a CMF as the time after
sectioning increases. This fact
indicates the definite value of de-
af ferentation for improving the re-
action to a CMF in comparison with
surgery.

The improvement of the EEG re-
action to a CMF in an isolated
brain was also expressed in a short-
ening of its latent period. While
in a normal rabbit, the mode in the
latent period distribution occurs
at 25 sec, in the isolated brain it
shifts to 15 sec (see Figure 39,

However, in the normal and

A). ,

isolated brain, we observe 2 basic/120

peaks in the latent period distribution curve of the EEG reactions to a CMF,
which can testify to the phase character of the reaction.

It is interesting to note that the reaction to turn-off in the isolated

102

brain was the same in stability and latent period as the same reaction in an in-
tact brain (see Figure 39, B) . This fact forces us to assume the presence of
different mechanisms for effectuation of the basic EEG reaction to a CMF and the
reaction to turn-off. Upon isolation of the brain, the mechanism of the basic
reaction changes somehow, but the mechanism of the reaction to turn-off remains
the same.

Thus, the structures of the diencephalon and telencephalon, deprived of
nervous connection with all receptors, react to a CMF sooner, more sharply and
more frequently than a normal brain. The question arose as to whether any sec-
tion of the brain will react to a CMF in an equal degree, or whether there are
sections of the brain more reactive to this stimulus. To answer this question,
we conducted experiments involving the exposure of a neuronally-isolated strip /122
of the rabbit cortex to a CMF and simultaneous recording of electrical activity
from several sections of the brain during the influence of a CMF.

The Effect of a QIF on _the Electrical Activity of a Neuronally-
isolated Strip of the Cerebral Cortex

m-'-*n f

SH % Wv^- ^"'v/v>-- Y^j.^^ V"-"--^"' iHli'-^'

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/123

r-S'^hr

"(;r'''^V/'^^^^^-J'r^//Pv^

(a) Abtxame-s-

/■/ 1

.. ■ ' \\ • . ..(b) 200mkB\^

Figure 43. Electrical Reaction to a CMF in a Neuronally-isolated Cortical
Strip and Adjacent Sections of an Intact Brain. A = Before Exposure; B, C =
During Exposure. The Numbers Designate Electrocorticogram Leads (See the
Diagram). Key: (a) Respiration; (b) 200 pv; (c) 1 Sec.

103

The method of Isolating the strip was described previously. In all, 7 rab- .
bits were given 157 l-to-3-minute exposures to a 200-Oe CMF. As is evident from
Figure 43, under Lue influence of a CMF, convulsive discharges sometimes appear-
ed in the strip later than in the other sections of the cortex, and sometimes
the reaction occurred only in the strip. There were cases when the electrical
activity of the strip did not change during exposure to the CMF. The reaction
stability was 46%. The distribution of the latent periods of the reaction in
the strip to a CMF (Figure 39, A) shows that the electrical reaction in the strip
occurs sooner than the EEC reaction of an intact brain.

The reaction to turn-off was observed in 53% of the cases; 12% of the cases
exhibited only the reaction to turn-off. The mode of the latent period distri-
bution of this reaction occurred at 5 sec. As is evident from Figure 39, in^
both the degree of stability and in the curve of the latent period distribution,
the reaction to turn-off in the normal brain, the isolated brain and the corti-
cal strip surprisingly coincided, although each total includes results from dif-
ferent rabbits; the data on the strip were obtained at a lower field strength.
We should also remember that in the experiments on the strip, the EEC reaction
was expressed most frequently in the appearance of convulsive discharges. Con-
sequently, a change in the state of the cortex (isolation of the brain or a
strip) affects only the form of the electrographlc expression of the turn-off
reaction, without changing its stability or latent period.

The above discussion on the form of the electrographlc reaction can also
pertain to the basic reaction, but its latent period and stability are more la-
bile than the turn-off reaction. The latent period of the reactions in the
strip and in the isolated brain preparation was shorter than in an intact brain,
although the differences in the degree of stability were insignificant.

We should note that the operation of isolating a strip also affected the
EEC reactions of the intact hemisphere under the influence of a CMF. At first,
convulsive discharges also began to appear there.

Finishing the analysis of this series of experiments, we must note that the
neuronally-isolated cortical strip changes its electrical activity during expo- /125
sure to a CMF. However, we cannot decide whether the observed changes in elec-
trical activity are a direct reaction of the cortical elements or whether they
are caused by humoral effects from various subcortical levels. Experiments in-
volving the simultaneous recording of electrical activity from several sections
of the brain can answer this question.

The Electrical Reaction of Different Sections of the Rabbit

Brain to a CMF

We have previously described the experimental method, the site where the
electrodes were embedded, and the form of the electrical reaction during a one-
minute exposure to a CMF. Therefore, we shall immediately go to the latent peri
od and the reaction stability, as characterized by the number of spindle-shaped
oscillations in cortical potentials. For our recording conditions (recording
speed, 15 mm/sec), the electrical reaction to a CMF almost always occurred in
all sections simultaneously (Figure 44). For 1,584 exposures involving 13 rab-

104

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5

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/^.W^

(b) Icen

Figure 44. Electrical reaction in the Cortex and Subcortical Formations
of the Rabbit Brain During the Influence of a CMF. The Arrows Designate
the Moments the Electromagnet is Turned On; A, B = Different Rabbits;
1 = EEG of the Frontal Region of the Cortex; 2 = EEC of the Occipital
Region of the Cortex; Electrograms of the Subcortical Formations; 5 =
Nonspecific Thalamus Formations; 4 = Hypothalamus; 5 = Reticular Forma-
tion of the Midbrain. Key: (a) 100 pv; (b) 1 Sec.

i 7,5 to 12, i « /<'5 20 ZZ,b 25
(\y\ HpfMu jiamnHnmoiO iiipunda ,ceii.

Figure 45. Distribution of the Latent Period
of the Electrical CMF Reactions Which Occur in
the Cortex and the Subcortical Sections of the
Rabbit Brain. Key: (a) Number of Reactions,
%; (b) Duration of the Latent Period, Sec.

bits, a reaction in just
the sensorimotor cortex was
noted in 25, in just the
parietal cortex in 6, in
only the hypothalamus in
30, and in only the reticu-
lar formation of the mid-
brain in 2. Figure 45 gives
the latent period distribu-
tion of the reactions taken
simultaneously from all
leads. We see that the
mode on the latent distri-
bution curve occurs at 5
sec. In comparison with
the curve obtained during
recording of the EEG reac-
tion to a CMF using elec-

105

trodes that were driven Into the bone (see Figure 39) , the latent period of the /126
reaction to a CMF in rabbits with embedded electrodes is significantly shorter
even several weeks after the operation, although the reaction stability (inclu-
ding unstable changes) was less (66 versus 95%). It is probable that the degree
of trauma inflicted by deeply embedding electrodes in the brain leads to a short-
ening of the latent period of the reaction to a CMF. We observed a similar ef-
fect during sectioning at the level of the midbrain and during isolation of a
cortical strip.

lau

JLDJl

n n n

300

ZOO-

wu-

n n

(a)

200-

W/l

The different latent periods of the reactions
to a CMF in different formations of the brain indi-
cate that the reaction in any section has a pro-
longed latent period, measurable in seconds. With
this type of experiment we could not clarify
whether the reaction is foirmed in all sections of
the brain simultaneously or whether it occurs at
some lead point, from which it is propagated to the
other sections of the brain via a neuronal pathway
(with a velocity that cannot be measured by an EEC
recording) . Experiments involving isolation of
the structures of the forebrain and the diencepha-
lon and with isolation of a cortical strip sway us
toward the first opinion, i.e., that the reaction
to a CMF is formulated independently in each sec-
tion of the brain, and then it occurs in the iso-
lated strip. Of course, the discussed pathways of
the reaction to a CMF do not exclude each other and
can coexist.

Figure 46. The Inten-»
slty (in %) of the Elec-
trical Reactions in Dif-
ferent Sections of the
Brain to a CMF in the
Norm (A), After Injection
of Caffeine (B) and Af-
ter Injection of Adrena-
lin (C) . 1 = Hypothala-
mus; 2 = Sensorimotor
Cortex; 3 = Parietal Cor-
tex; 4 = Specific Nuclei
of the Thalamus ; 5 = Non-
specific Nuclei of the
Thalamus; 6 = Hippocam-
pus; 7 = Reticular Forma-
tion of the Midbrain.
Key: (a) Intensity, %.

In any case, if the different sections of the
brain react relatively identically to a CMF in
their latent period, there is a definite hierarchy
in the intensity of the reaction, i.e., the rela-
tive (%) increase in the number of spindles during
exposure in comparison with the background record-
ing.

Figure 46 shows that normally the most inten-
sive reaction to a CMF is observed in the hypothal-/127
amus, then come the sensorimotor cortex, the pari-
etal cortex, the specific nuclei of the thalamus,
the nonspecific nuclei of the thalamus, the hippo-
campus and the reticular formation of the midbrain.
Based on these results, we can consider that just
the hypothalamus is the lead point in the reaction
to a CMF.

However, an intramuscular injection of caf-
feine, 50 mg/kg, into 3 rabbits changed the inten-
sity of the reaction to a CMF. The intensity in-
creased in all leads, but the reaction of the cortex, especially the sensorimo-
tor cortex, most sharply increased; the hypothalamus then occupied third place.

106

followed by the specific nuclei of the thalamus, the reticular formation of the
midbrain, the hippocampus and the nonspecific nuclei of the thalamus.

Intravenous injection of adrenalin, 0.03 mg/kg, into 3 rabbits also increas-
ed the intensity of the reaction to a CMF, only here the reticular formation of
the midbrain, usually in last place, became the "lead point," followed by the
hypothalamus, sensorimotor cortex, parietal cortex, specific thalamus, hippo-
campus and nonspecific thalamus.

Thus, the results of experiments involving the injection of caffeine and
adrenalin testify in favor of the viewpoint that the CMF acts on each section of
the brain, but the intensity of the reaction is determined by the reactance of
the separate sections.

By artificially increasing excitability by means of pharmacological agents,
we can arbitrarily make one or another section of the brain more reactive to
CMF. According to pharmacological data [Val'dman, 1963], caffeine acts essen-
tially on the cortex, and adrenalin, on the reticular formation of the midbrain.
According to this we have obtained a greater intensity of the reaction to a CMF
in the cortex or in the reticular formation of the midbrain.

Probably the hypothalamus and the cortex normally are the most reactive
structures and, therefore, react most intensively to CMF.

The experimental results of R. A. Chizhenkova, who found that the electri-
cal reaction of the cerebral cortex to CMF did not change significantly after
destruction of the hypothalamus, thalamus or reticular formation of the midbrain,
also indicates the absence of "lead points" in the reactions to CMF.

Turning to the results of experiments on an isolated cortical strip, we can
now assume, with a better basis, that the observed changes in the electrical ac-
tivity of the strip are caused by the direct effect of a CMF on it. What cellu-
lar elements in the cortex react to a CMF? We have proposed that recording the
impulses of the cortical neurons during exposure to a CMF can answer this ques-
tion.

The Effect of a CMF on the Pulsed Electrical Activity /128

of Cerebral Neurons

S. N. Luk'yanova made an extracellular recording of the electrical activity
of 352 neurons of the sensorimotor cortex, parietal cortex, hypothalamus, speci-
fic formations of the thalamus, hippocampus and the reticular formation of the
midbrain in tests on 5 rabbits. Exposure to a 1,000-Oe CMF on the animal's head
lasted one minute. The electrical activity of the neurons was continuously re-
corded for 20 sec. The recording periods were distributed over time as follows.
The first 20-second interval was recorded with no exposure, then followed a 30-
second interval. The second recording included 10 seconds of background and 10
seconds after the electromagnet was turned on. The third recording, which char-
acterized the influence period, was taken after a 10-second interval. The fourth
recording, beginning after a 10-second break, contained 10 seconds of exposure,
the moment the magnet was turned off and 10 seconds of the aftereffect. The

107

fifth and last 20-second recording, which characterized the aftereffect period,
was taken after a 30-second break. The results of two or more exposures on cer-
tain neurons were recorded =

Such an experimental method allowed a comparison of the results obtained by
the microelectrode technique with the results of EEG investigations, in which a
continuous 3-minute recording of the electrical activity was made with the peri-
od of field influence located in the middle minute.

In treating the experimental results, the number of spikes for each 10-
second recording was counted in such a way that 10 numbers were provided for
each test. Table 14 shows the additive results for the number of spikes for
neurons in different sections of the brain. This addition makes it possible to
display reactions of cortical neurons to stimulation, in particular to light
stimulation [Kondrat'yeva, 1964].

TABLE 14. TOTAL NUMBER OF RECORDED DISCHARGES FROM NEURONS IN DIF-
FERENT SECTIONS OF THE RABBIT BRAIN DURING 10-SECOND INTERVALS IN
THE BACKGROUND, DURING EXPOSURE TO A CMF, AND AFTER THE EXPOSURE.

7129

Section of
the brain

o o

M
• 3
O (U

g a

Background

Influence period

Aftereffect period

hypothalamus

2804

2865

2826

2620

2832

2715

2788

2679

2339

2650

hippocampus

1621

1632

1666

1747

1731

1903

1606

1805

1640

1903

reticular for-
mation of the
midbrain

2495

2060

2690

2553

2061

1980

2376

2228

2362

2201

specific nu-
clei of the
thalamus

36-

1546

1593

1799

1663

1626

1965

2089

2915

2761

2636

sensorimotor
cortex

3369

3346

3378

3329

3404

3853

3482

3710

3468

3384

parietal cor-
tex

3829

3727

3715

3895

4041

3900

3731

3621

3406

3501

total

352

15664

15223

16074

15807

15695

16316

16072

16958

15976

16275

As the lower line of Table 14 shows, during the influence of the CMF, there
were no significant changes in neuronal impulses. This conclusion is valid not
only for the total data, but also for the separate sections of the brain. Only
in the thalamus do we note a tendency toward an increase in neuronal impulsation
after the electromagnet is turned off.

Comparing the additive results for neuronal impulses with the results of a

108

similar addition (counting the number of spindles and slow waves) of the elec-
trical activity recorded by microelectrodes in the same sections of the brain,
we see that the electrical activity of the brain tissue changes during the in-
fluence of a CMF, but the total number of neuron spikes does not undergo a
change. Hence, the conclusion follows that neurons perhaps do not participate /130
in the initial reaction of the brain to a CMF. We shall put aside a final judg-
ment on this question until we have obtained results from different methods of
treating the data.

In studying the reaction of separate neurons to different stimuli, investi-
gators quite frequently divide the neurons into groups. In these experiments,
neurons were considered excited if the frequency of their impulses increased
twice every 10 seconds (group I) . The same quantitative reduction in the impulse
frequency characterized inhibited neurons (group II) . All the remaining neurons,
which did not significantly change their impulse frequency, were placed in the
third group. We have treated the results obtained both during the influence of
a CMF and in its absence according to this classification. The change in the
number of neurons that retain their impulse frequency during exposure to a CMF
allowed us to make a conclusion about the presence of a reaction.

The obtained data are given in Table 15. First of all, we should note that
in comparing the average electrical activity of neurons for all sections of the
brain without the influence of a CMF (the first and the last columns), the num-
ber of group III neurons varied slightly (65 and 70% respectively). The averages
characterizing the sections of electromagnet turn-on (74%) and turn-off (79%)
are close to the previous figures, which indicates the absence of significant
changes in the activity of neurons during changes in the CMF. However, in com-
paring both the average data and the results of investigating each section of
the brain separately, which were obtained by recording 20-second intervals of
neuronal impulsation without the influence of a CMF as well as during the middle
period of the influence (the third column of the table), we see that the number
of neurons in group III decreased approximately twice. This fact indicates that
the impulses of cerebral neurons acquire a longer latent period (more than 10
sec) under the influence of a CMF.

A comparison of the results of the first and third columns of Table 15
makes it evident that under the influence of a CMF, the number of group III neu-
rons changes most in the hypothalamus (by 33%) and least in the reticular forma-
tion of the midbrain (by 8%), which coincides with the data on the reactance of
these formations to a CMF, obtained by means of recording the electrical activ-
ity with microelectrodes.

The decrease in the number of group III neurons is accompanied by an ap-
proximately identical increase in the number of group I and II neurons, which
coincides with the data of Table 14 and testifies that the total activity of neu-
rons does not change under the influence of a CMF. We should note that in tests
on 7 rabbits, L. L. Pragina observed similar reactions to a CMF from 28 neurons
of the sensorimotor and parietal regions of the cortex.

Investigators usually base the grouping of neurons on several reactions of /131
the same neuron. Since the exposure was prolonged in our experiments, we rarely
managed to record the results of two CMF exposures on one neuron. Twenty-three

109

TABLE 15. THE RELATIVE NUMBER OF EXCITED (I), INHI-
BITED (II) AND UNCHANGED-ACTIVITY (III) NEURONS IN
DIFFE5ENT SECTIONS OF ToE BRAIN IN THE BACKGROUND, DUR-
ING EXPOSURE TO A CMF, AND AFTER EXPOSURE.

Section of the brain

(U o

3 a)
z a

CO
Ou

3
O

u
o

Number of Neurons, %

•iH

a a

3--S 3— V

O U O U

Vi 0) l-i 0)

60 CO bO CO

o o oo

nj rH (di-i

•rl

3/~v (U,^
O O M O

M 0)
bOO]

3 <U
CO CO

oo 0.0

<l)v

•iH

3^
o u

tlO CO

o o

Cd CM

h u

3 <U

CO CO

•H 4J

S o

Vj U tt-l O
3 0) (U 0)
CO CO M CO

o <u
cuo ■uo

nj^

o >

14-1 CJ 14-1 U

0) (U <U (U

M CO )-l CO

0) 0)

4JO W O

14-1 iH M-lrH

hypothalamus

hippocampus

reticular formation
of the midbrain

specific thalamus

sensorimotor cortex

parietal cortex

averages

III

12
22
66

13
13
74

22
28
50

9
21
70

16
17
67

18
17
65

15
20
65

6
14
80

22
22
56

20
19
61

19
14
67

13
13
74

24
43
33

30
25
45

26
32
42

24
29
47

25
31
44

24
36
40

25
33
42

18
14
68

8
14
78

7
12
81

9
12
79

11
10
79

2
25
73

14
12
74

26
23
51

16
14
70

neurons received 2 exposures: 7 of them did not change their activity either
time, 8 neurons changed their activity only under one of the two Influences, 4
neurons decreased their Impulsatlon both times, and 2 Increased It. Thus, to
Increase the clarity of neuron classification. In the future we must apply a
larger number of exposures to the same neuron.

110

zoo

^fBO

\I2D

» A

• \M :

^ 80

t ^ V

(a)

■i V

^v^-.

Figure 47. Changes in the Electrical Activity of Neurons
at Turn-On (B) and Turn-Off (A) of the Electromagnet.

We have already mentioned that /132
turning the electromagnet on and off
does not cause sharp changes in the elec-
trical activity of neurons (for example,
see Figure 47) , both in the addition of
neuronal activity, as well as in neuron-
al grouping. However, the data of Table
15 show that while the reaction of neu-
rons does not occur in the first 10
seconds of the CMF exposure, the after-
effect lasts for the first 10 seconds
after the electromagnet is turned off.

A comparison of the electrical re-
actions of the brain and separate neu-
rons to a CMF shows that in both cases
the reaction is distinguished by a long
latent period and by a prolonged after-
effect. The changes in electrical brain
activity during the influence of a CMF
were clearer than the changes in neuron-
al impulsation. These facts force us to
assume that not only the bodies of nerve
cells, but also other formations of nerve
tissue (dendrites, glia or blood vessels)
participate in the electrical reaction
of the brain to a CMF. The latent peri-
ods of the neuron reactions are usually
measured in milliseconds. In our case,
this index is increased thousands of
times, forcing us to think that neurons
react late to a CMF.

800 600 fOO ZOO B ZOO -fOO 600 800
(b) BpeMO ^ Mcen

Figure 48. Frequency of Neuronal
Impulsation to a Light Flash in the
Background (1), During Exposure to a
CMF (2) and After the Electromagnet
was Turned Off (3). Key: (a) Num-
ber of Impulses; (b) Time, msec; (c)
Light.

Let us recall that the electrical reaction of the brain to a CMF was ex-

Ill

pressed most clearly when test light stimuli were used. This fact served as the
basis for experiments conducted together with I. N. Kondrat'yeva that involved :
recordiug the pulsed activity of neurons LhaL react Lo light. Tne recording
lasted 2-3 sec. We investigated the reaction of 23 neurons in the visual cortex
to a light flash before, during and after exposure to a 200-Oe CMF on the am- /133.
mal's head. During each stage of the investigation, we administered 106 flashes.

The total dynamics of neuronal activity are given in Figure 48. Analyzing
the curves before the start of the light effect, one can see that the number of
neuronal impulses increased somewhat under the influence of the CMF, but this
increase was not statistically reliable (p > 0.05). The increase in neuronal
impulsation after the electromagnet was turned off was statistically reliable
(p < 0.05).

The neuronal reaction to a light flash intensifies both during exposure to
a CMF and, in particular, after the electromagnet is turned off.

The obtained results show that a CMF affects neuronal activity in a partic-
ularly strong way during the functional loading caused by light stimulation. A
CMF causes a sharply expressed aftereffect. In control experiments on 36 neu-
rons, without exposure to a CMF, we did not observe a dependence of the reaction
to the flash on the number of preceding flashes.

Discussion

/134

In studying the electrical reaction of the rabbit brain to a CMF, we re-
vealed its significant similarity to the reactions to UHF and SHF fields. It is
possible that the physical basis of this similarity is the fact that the magnet-
ic component of EMF has a more significant biological effect [Khazan and
Goncharova, 1959; Presman, 1960; Nikonova, 1963, and others]. On the other hand,
the probability of the presence of a nonspecific physiological reaction of the
CNS to any penetrating physical factor is not precluded. We should note that a
CMF is a weaker factor than a UHF or SHF field. The stability of the EEG reac-
tion increases as the field strength increases, but at 1,000 Oe it does not ex-
ceed 52%.

It is possible that oscillations at the metabolic level, which are reflec-
ted in superslow oscillations of brain potential [Aladzhalova, 1962], can ex-
plain the statistical nature of the electrical brain reaction to different
fields. Depending on the phase of the potential oscillation, the sensitivity^to
a CMF changes, and we may not obtain a response, although the external experi
ment conditions remain identical. As the number of exposures Increases, the
probability increases of coincidence of the exposure with increased excitability
of the brain 6y a CMF, which leads to a statistically reliable change in elec
trical brain activity.

Our observations show that a predominance of spindles and slow waves in the
background reduces the probability of the appearance of a reaction to a CMF. If
we artificially increase the number of spindles or slow waves on the EEG by intra-
muscular injection of 12 mg/kg of aminazine or by intraperitoneal injection ot
13 mg/kg of sodium amytal, then the electrical reaction of the brain to a CMF

112

disappears. As we have already said, artificially increasing the excitability by
injection of caffeine or adrenalin significantly intensifies the reaction of the
CNS to a CMF. Consequently, tests involving the injection of pharmacological
substances support the assumption that the statistical nature of the electrical
brain reaction to a CMF can depend on unconsidered variations in the excitabil-
ity of the nervous system.

However, if the effect of a CMF depends on variations in brain excitability,
we can "capture" increased excitability by increasing the exposure time to the
CMF. Experiments of this type were conducted on 4 rabbits, from which we re-
corded the EEC of the sensorimotor and visual regions of the cortex by the mono-
polar method with electrodes embedded in the bone. The CMF attained a strength
of 300 Oe and acted on the animal's head for 3 hours a day. The experiment
lasted 2 weeks.

Under the prolonged influence of a CMF on the head of a rabbit, the EEC /135
changed differently than during brief exposures to the same field, and the
changes were not limited to just the electrical brain activity. The treatment
of the results was not as thorough as for the brief exposures, but a tendency
was revealed toward predominance of low-amplitude (less than 50 yv) potentials
on the EEC both during exposure to the CMF and in the background recording, as
the number of prolonged exposures increased (Figure 49). We should note that on
the 8th day, high-amplitude (above 100 \iv) potential oscillations predominated,
but later on the biopotential amplitudes began to decrease. The observed am-
plitude changes in the EEC during exposure to a CMF resemble the phase reaction
of the cerebral cortex to a weak dose of ionizing radiation [Livanov, 1962].

Besides the changes on the EEC, we observed an increase in excitability in
these rabbits, manifested as a marked increase in motor reactions and a reduc-
tion in total body weight by 9-17% during the two-week period. The control rab-
bits, which were also harnessed on the stand for a long time and subjected to
the influence of the same CMF, but for 1-3 min with an interval of 10-15 min,
did not exhibit changes in weight or motor activity, and their EEC changes were
of a different type, which we have described previously.

The obtained results cannot be explained only by the fact that during pro-
longed exposure to a CMF, the effect depends to a lesser degree on variations in
brain excitability. While the reaction to each subsequent brief exposure to the
CMF did not depend on the preceding brief exposure, during prolonged exposures
we observed a clear summation effect. It is probable that the repair processes
in the CNS cope quickly and easily with the degree of alteration inflicted by a
1-3 minute exposure to a CMF. However, the repair processes are significantly
disturbed after a 3-hour exposure to a CMF since a 24-hour break in the field
does not allow restoration to the initial EEC. The described results show that
we have still devoted little attention to the duration of the EMF exposure. In
subsequent investigations, consideration of this factor will promote a deeper
analysis of the mechanism of the effect of EMF on the CNS.

Recording the electrical activity with macroelectrodes and microelectrodes
from different sections of the brain showed that the reactions to a CMF, as to
a UHF or an SHF field, occur with a long latent period for all the structures
recorded. However, the hypothalamus occupies first place in reactance to a CMF.

113

7136

3 0)

^ -^

"-1 ^

tIJ «

O 0)

CO (U

•N S-^

•^

< CO

s^ T3

>-i (U

3 hJ

Sri o

1 w

CO W

cd 0)

-G

H u

4J 0)

H-l w

<: Cd

X -H

(U 13

*J C

)-i M

O CO

U (U

•H ^

j3 a

,Q 3

•

iw •

o b

^-v

>^o

4-1

^-^

•H cd

• M

.rl (3

4J -rl

•al >>0

.-1 u

csl

cd en

/^N

•H /-N

n o

N-^

^ v_^

a) Vj

• •

r^ 3

w o

• o

C3^ vO

•

■^

^-s

)-i cd

t>0

60^

•rl P3

•zi

Pm ^^

It is possible that this section of the brain is also more sensitive to EMF, but
we will make our final judgment on this conclusion following experiments which /I 3,7
determine the CMF thresholds for different sections of the brain. At this time
it is appropriate to note that the hypothalamus, where the higher autonomic cen-
ters are located, is considered the structure most reactive to the effect of
penetrating factors [Popov, 1940; Aladzhalova, 1962; and others].

The cortex follows the hypothalamus in reactance to a CMF. After prolonged
exposures to high-frequency [Tolgskaya, Nikonova, 1964], UHF [Shvarts, 1945] and

114

SHF [Pitenin. 1962; Tolgskaya, Gordon, 1964] fields, morpho legists have found
the most significant histological changes in the neurons in just these sections
of the brain.

Recording neuronal impulsation has shown that, in contrast to such stimuli
as light, sound or an electrical current, a CMF causes a neuronal reaction with
a longer (more than 10 sec) latent period. It is possible that the neuronal re-
action involves a secondary means, i.e., the CMF causes some changes in the me-
tabolism of the brain tissue and the neurons react to this chemical factor. The
absence of change in neuronal activity at turn-on and turn-off, and also the
existence of an aftereffect, speak in favor of this assumption.

The changes in the electrical activity of the rabbit brain indicate the
presence of a biological effect of a CMF. This effect was intensely revealed by
means of test stimuli (the reactance curve and the reaction of visual cortex neu-
rons to a light flash) and by increasing the excitability by pharmacological (in-
jection of caffeine or adrenalin) and surgical (isolation of a cortical strip)
means.

Similar changes in the electrical brain activity during exposure to a CMF,
which were manifested as an increase in the number of slow high-amplitude oscil-
lations, were observed in salamanders [Becker, 1963] and in humans [Dinculescu
et al., 1963; Vyalov et al. , 1964].

Thus, both our data and the reference data indicate the effect of a CMF on
the CNS.

Conclusions

1. During a 1-3 minute exposure to a 200-1, 000-Oe CMF on the head of a
rabbit, we observed a statistically reliable increase in the number of spindles
and slow high-amplitude oscillations in the electrical brain activity. The la-
tent period of this basic reaction varied from 5 to 100 sec, and the stability
attained 52%. A brief desynchronization reaction sometimes occurred in the cor- /138
tex at the moments the electromagnet was turned on and off. After the electro-
magnet was turned off, we observed an off-effect with an average latent period

of 15 sec that resembled the basic reaction in the form of the changes in elec-
trical activity. The time for the appearance of assimilation to light flashes
of increasing brightness increased during exposure to a CMF on the animal's head.

2. The electrical reaction of the cortex to a CMF occurred more frequent-
ly, more intensely and with a shorter latent period after sectioning at the lev-
el of the midbrain. The reaction to a CMF was also retained in a neuronally-
isolated cortical strip.

3. Experiments with embedded electrodes showed that all the recorded sec-
tions of the brain react to a CMF. The most reactive were the hypothalamus and
cortex, and the least reactive was the reticular formation of the midbrain.

4. By recording the neuronal impulsation in different sections of the
brain, we observed that a CMF does not change the total activity of all the

115

studied neurons, but with a latent period of more than 10 sec it inversely re-
duces the number of neurons that do not change their activity. Under a CMF, the
number of excited and inhibited neurons increases by approximately tne same ae-
gree The impulsation reaction of visual cortex neurons to a light flash during
exposure to a CMF increased its intensity. This effect was retained m the af-
tereffect.

5. A CMF causes a nonspecific electrical reaction in the rabbit brain that
is similar to the reaction which occurs during exposure to UHF and SHF fxelds.

116

CHAPTER 4. THE EFFECT OF M ELECTROSTATIC FIELD ON THE

RABBIT EEC

The biological effect of an electrostatic field (ESF) has been studied
since the invention of an electrostatic machine by von Gerich in 1672 up to the
present time [Ostryakov, Vorob'yev, 1964; Solov'yev, 1962], but the mechanism of
this effect remains unclear [Anikin, Varshaver, 1950]. It is considered that
the physiological effect of an ESF on the nervous system is accomplished by
means of reflexes. By stimulating the endings of the trigeminal and other
nerves, an ESF can cause changes in the functional state of the CNS. Airborne
ions formed in a silent discharge reach the mucosa of the respiratory tract and
skin and have a stimulating effect on their receptors. With this we observe a /139
change in the skin sensitivity, stimulation of capillary blood circulation, nor-
malization of the vascular tonus, a change in the morphological state of the
blood, and improvement in gaseous interchange during the activity of the gastro-
intestinal tract [Finogenov, 1963]. In physiotherapy, the method of therapeuti-
cally using an ESF is called franklinization. We should remember that, besides
an ESF, the airborne ions and ozone formed in an ESF can affect animals and man.
However, we shall only be interested in the effect of an ESF on the rabbit EEC
during a local one-minute exposure to this factor on the animal's head employing

I . • f

Z '^*'f*J'SJ\^>Kr--i'i*Ai/A^

>JAwyvsrvAA*wW

2 >''''*V^''*AAAi(A^»*\^v>^^

i •:.- '■ (a) mrndfL^

Figure 50. Change on the Rabbit EEC During the Influ-
ence of an ESF on the Animal's Head. Continuous Record-
ing. The Arrows Designate the Moments of Generator Turn-
On and Turn-Off; 1 = Sensorimotor Cortex; 2 = Visual Cor-
tex. Key: (a) 100 yv; (b) 1 Sec.

117

brief intervals (1-5 min)

"~\ \

§Zfl

lA >k

:g to

(a)

//
/ 1
/I

v. — -

1 1

2 H 6 B

Figure 51. Distribution of the
Duration of Desynchronization Reac-
tions at Turn-On (1) and Turn-Off
(2) of an ESF. Key: (a) Number of
Reactions, %; (b) Duration of Reac-
tions, Sec.

We investigated the effect of an
ESF on the rabbit EEG together with N.
A. Solov'yev. As in the preceding
tests, we recorded the EEG by the mono-
polar method from the sensorimotor and
visual regions of the cortex. The field
strength, as recorded by a voltmeter,
was 1.25, 2.50 and 5.00 kv/cm. The dura-
tion of exposure was one minute.

First of all, we found that a de-
synchronization reaction frequently oc-
curred in the rabbit cortex when the ESF
was turned on and off (Figure 50) ; this
reaction occurred at field strengths of
5.00 and 2.5 kv/cm, but was not observed
at 1.25 kv/cm. Consequently, the ESF
threshold for the desynchronization re-

action lies between 1.25 and 2.50 kv/cm

A detailed analysis of the desynchronization reaction during the influence
of a 5.00 kv/cm ESF showed that this reaction occurred at turn-on in 61% of the
cases, and at turn-off, in 31%. The latent period of these reactions was equal
to a fraction of a second and, therefore, by recording the EEG on a recording
electroencephalograph we could not measure it. The duration of the reactions
varied from 1 to 11 sec, but most often it was 3-4 sec. Figure 51 shows dis-
tribution curves of desynchronization reactions with different duration at turn-
on and turn-off of the ESF. The similarity of these curves, on which the mode
is at 3 or 4 sec, draws our attention. From this we can conclude that the de-
synchronization reactions at turn-on and turn-off of the ESF are caused by a
similar mechanism. The only difference is that the reaction occurs twice as
frequently at turn-on as at turn-off. In this sense the effect of an ESF re-
sembles the effect of an SHF field, in which the reaction at turn-on occurred in
31% of the cases and at turn-off in 12%. However, the desynchronization reac-
tions during the influence of an ESF occurred twice as frequently as during the
influence of an SHF field.

An analysis of the dynamics of the number of spindles and slow waves on the
EEG, which allowed us to reveal the basic reactions to the previously investi-
gated CMF, showed that, in contrast to the effect of a 1,000-Oe CMF, no statis-
tically reliable changes occurred in the rabbit EEG at ESF exposures of 1.25 and^M
5.00 kv/cm (Figure 52). Only the desynchronization reaction at turn-off of the
5.00 kv/cm ESF reveals a drop in the number of spindles and slow waves on the
EEG in the first 10 sec of the exposure. Even this drop did not occur during
the influence of the 1.25 kv/cm ESF. The results of the ESF experiments given
in Figure 52 can serve as a control for tests with a CMF, and once more indicate
the presence of an EEG reaction to a CMF.

It is possible that M. N. Livanov et al. (1960) studied the effect of an
ESF on the rabbit EEG that occurred when an x-ray machine was turned on. We

118

I 5 /J ZS 35 15 55 \ 5 IS 25 35 H5 55\5 IS ZS 35 iS SS

/■L^s BpcMn , eeir.

Figure 52. Dynamics of the Number of Spindles
and Slow Oscillations of Rabbit Cortical Poten-
tials in the Background and During Exposure to
a CMF (1) and an ESF with a Field Strength of
5 kv/cm (2) or 1.25 kv/cm (3). A = Before Ex-
posure; B = in the Influence Period; C = In the
Aftereffect. Key: (a) Number of Spindles, %;
(b) Time, Sec.

during exposure to an ESF.

conclude this from the report
concerning the presence of a
desynchronization reaction
with a duration of 2-8 seconds
during an 8-15 second exposure
to the field — this reaction
occurred less often in our
experiment involving other
fields — and from the simil-
arity of the threshold values
of the field. In the stated
work, the effect is reduced
when the cable is at a dis- /142
tance from the animal, and
also when the voltage in the
machine is lowered from 60 to
10 kv.

Thus, the effect of an
ESF on the rabbit EEG is of a
different type than the effect
of high frequency, UHF, SHF
and CMF.

Conclusions

1. A one-minute exposure
to a 5 kv/cm ESF on the head
of a rabbit caused a desyn-
chronization reaction in the
electrical activity of the
cortex at the moments the
generator was turned on and
off. Significant changes
were not observed on the EEG

2. In contrast to UHF, SHF or CMF, an ESF did not increase the number of
spindles and slow oscillations of potential in the cortex either during the ex-
posure or after it.

Synopsis

At the end of the part devoted to the electrographic method of investigat-
ing the effect of EMF on the CNS, we should review the results described in the
preceding chapters.

Although we used EMF of different ranges and different intensities, never-
theless we obtained similar results by using the same methods of investigation.
The similarity was observed in the form of the EEG reaction: the low stability

119

and the long latent period of the reaction, the existence of desynchronizatxon
reactions that occur at the moments of generator turn-on and turn-off. the pres-
ence of reactions to turn-off, the direct effect of EMF on the structures of the
telencephalon and diencephalon, and also on an isolated cortical strip. Since
we did not set ourselves the task of a detailed comparison of the EEG reactions
to different EMF at the start of the investigation, there may be certain omis
sions in the discussion of these questions, but the general picture is sufti
ciently clear.

The Form of the EEG Reactions

It turned out that during exposure to a field, there was a statistically
reliable increase in the number of spindles in the anterior sections of the cor
tex, and of the number of spindles and slow waves in the posterior sections.
During the influence of UHF and SHF fields, we frequently recorded the EEG only
from the posterior sections of the cortex and analyzed only the slow waves. How-
ever, because of the diffuse nature of the EEG reaction to EMF and the function-
al relationship of spindles and slow waves, the reactions which we evaluated
?rom different indices and in different sections of the cortex had much in commai.

The appearance of slow waves on the EEG and an increase in potential ampli-ZUS
tude are most frequently described in the literature as the result of the ettect
of different EMF. However, other forms of the reaction have also been observed.
Sh. K. Pardzhanadze (1954) noted a quickening of the waves and an increase in
their amplitude on the rabbit EEG during exposure to a UHF f^^^*^- . ^- ^^"""-^^^^^^
(1954) noted an increase in voltage and bursts of theta-waves on the EEG of some
people. M. S. Bychkov (1957) and I. N. Zenina (1964) reported the appearance of
a different form of change in rabbit and cat EEGs depending on the power flux
density and the duration of the exposure to an SHF field. M. Baldwin et al.
(1960) noted the appearance of slow waves (a rhythm of 5 Hz predominated) on the
simian EEG during exposure to a 225-400 MHz SHF field on the animal s head.

Slow waves and spindles appeared on the rabbit EEG in our tests during the
influence of a UHF field [Kholodov and Yanson, 1962a, b], a constant [Bavro and
Kholodov, 1962] and a pulsed [Kholodov, 1962a] SHF field, and also during the
influence of a CMF on the animal's head [Kholodov, 1963a]. An increase in the
amplitude of slow waves on the rabbit EEG also occurred under the influence ot a
high-frequency field (500 kHz) [Nikonova, 1963].

Thus, the enumerated results of a few experimental works on the effect of
EMF of different ranges on the EEG show that in the majority of cases the reac-
tion was manifested as an increase in amplitude, but sometimes as ^ /^"^^^J^'^
frequency of the cortical potentials. Many investigators have noted that this
reaction is diffuse in nature and has a significant aftereffect. The majority
of investigators recorded the EEG after exposure.

Clinical studies involving the recording of the EEG from people who have
undergone prolonged exposure to different EMF in industrial conditions are a
somewhat isolated phemonenon. All such works presently testify that patnoiogi
cal forms of bioelectric activity are recorded in the EEG of these subjects in
the form of slow delta-waves and beta-waves, frequently sporadic in character
[Drogichina et al., 1962; Klimkova-Deycheva and Rot, 1963; Svacina, 1963;

120

Ginzburg, 1964; Vyalov et al. , 1964]. We should note that, soon after different
total-body x-ray irradiation of rabbits, there was an increase in the amplitude
of bioelectric oscillations, and sometimes changes in the frequency spectrum of
different directivity, in the cerebral cortex [Livanov, 1962].

In sum, we find that the most diverse EMF cause similar nonspecific changes
on the EEG of different vertebrates. Since the primary biophysical processes
are different during exposure to different EMF, we assume that the described EEG
reaction reflects a common final result of the interaction of the organism with
the field. Let us explain this by means of an example. The sensation of phos-
phene in man can be caused by adequate (light), electric and mechanical stimu- /144
lation of the retina. However, it does not follow that the enumerated stimuli
act on the retina in an identical manner.

What is the functional significance of the forms of the EEG reactions that
are observed most frequently during exposure to EMF? It is known that slow waves
and spindles appear in the cortex of sleeping animals. Therefore, it is reason-
able to asstime that EMF cause inhibition in the CNS. This assumption is sup-
ported by certain facts, for example: the results of our tests on determining
the threshold of assimilation for a rhythm of flashing lights of increasing
brightness in rabbits during the influence of a CMF; the statement regarding
somnolence in certain people who work in EMF [Grigor'yev, 1881; Klimkova-Deycheva
and Rot, 1963]; the lowering of the sensitivity in an electrical current during
exposure to a magnetic field on the human occiput [Nikolayev, I960]. However,
although EMF have a predominantly inhibiting effect on the CNS during normal
conditions, when there is an increase in excitability due to injection of caf-
feine or mechanical stimulation of the brain, EMF can cause convulsive discharges
on the EEG, which is an index of the limiting excited state.

In the partially discussed experiments on the effect of a SHF or a constant
magnetic field on the rabbit EEG in which a cortical strip was isolated in the
sensorimotor region of the left hemisphere, the following interesting fact ap-
peared.

TABLE 16. CHARACTERISTICS OF THE EPI-
LEPTOGENIC EFFECT OF DIFFERENT STIMULI,

Stimulus

4-1
O

U 4J

4-1 M
O <]}
U
U 3
0) M

-9 ° ■

3 ><!

Number of con-
vulsive electro-
graphic reactions

Stability of con-
vulsive electro-
graphic reactions, %

basic

turn-
off

total

basic

turn-
off

total

200-0e CMF

200 mw/cm2 SHF field

light

sound

7
5

7
7

109
26
32
44

73
17

15
8

19
3
5
8

92
20
20
16

67
65
47
18

17
12
16
18

84
77
63
36

121

Apart from the stated fields, we used light from an electric light and
sound from a ZG-10 generator as stimuli. These stimuli were of moderate strength, :
but they always caused a desynchronizgtion reaction in the EEG of an intact rab-
bit brain. However, during their influence on a damaged brain these stimuli
were less epileptogenic factors than EMF. The experimental results are given
in Table 16.

The far right of the table shows that the most epileptogenic stimulus for /145
the intact brain was the weakest stimulus, the CMF, followed by the SHF field,
light and, finally, sound.

These experiments clearly demonstrate the presence of the effect of EMF on
the CNS. They show that discussions on the weak nature of EMF as stimuli, and
on their predominantly inhibiting effect, pertain only to the normal functional
state. If, however, the excitability is increased, if by additional exposures
we cause a paradoxical parabiotic phase in the CNS, EMF cease to be weak and in-
hibiting stimuli and provoke the strongest excitation. In connection with this,
we should note that the seizures of so-called "television epilepsy" [Pallis and
Louis, 1961; Mawdsly, 1961] that have occurred in certain people when they tuned
a television set are possibly provoked not only by the light stimulus, but also
by the electromagnetic stimulus that is more intensive close to the set.

The Reaction to Turn-Off

Table 16 shows that, in contrast to the basic reaction, the electrographic
reaction to turn-off occurs in an almost equal number of cases after different
stimuli are turned off. The reaction to turn-off was an unexpected event. It
was first noted in tests on the effect of a UHF field on an isolated cortical
strip. In experiments involving the effect of an SHF field on electrical brain
activity, it was shown that this reaction actually exists, and in investigations
of the effect of CMF on the CNS, it was described with sufficient detail from a
quantitative point of view.

First of all, the existence of reactions to turn-on and turn-off leads to
the thought concerning the similarity of such reactions to the regularities es-
tablished for a direct current acting on a neuromusculature preparation. The
difference was that during exposure to an EMF, reactions were observed not only
for a change in the field, but simply for its presence as well.

The functional significance of the reaction to turn-off remains unclear not
only in our experiments on the effect of CMF on the CNS, but also in the discus-
sion of most of the presently accumulated facts on this question [Granit, 1957].
It seems that the presence of a system with a turn-on and turn-off effect char-
acterizes many receptor formations, but it occurs most clearly in the reactions
of the visual receptor. It is assumed that the turn-on and turn-off effects
are connected with the activity of different mechanisms. In certain cases, the /146
turn-off effect is absent on the periphery of the analysor (the Limulus and the
mammal auditory organ), but it can appear in its central formations. It is pos-
sible that an impulsation in response to turn-off, similar to the so-called
Sherrington release reflex, can occur as a result of the interaction of central
structures that have a contradictory effect on one and the same neuron [Granit,
1957].

122

The difference between our results and classical concepts regarding the ef-
fect of turn-off is that, like the basic reaction to EMF, the reaction to turn-
off is effected by some slow system, since the latent period of the reactions
is measured in seconds.

A second difference is observed in the identical form of the changes on the
EEG during the basic reaction to EMF and during the reaction to turn-off.

On the other hand, our results can indicate a certain independence of the
mechanisms responsible for the basic reaction and the reaction to turn-off. One
reaction can be realized regardless of the presence or absence of the other.
Although the stability of the reaction to turn-off was usually less than the
stability of the basic reaction, we cannot say that turn-off of the EMF is the
weaker stimulus. In the experiments whose results are shown in Table 16, im-
mediately after isolation of a cortical strip we did not observe a convulsive
EEG reaction to EMF. After some time has passed, convulsive discharges appeared
on the EEG only after the EMF was turned off. Then, for a long time, convulsive
discharges appeared both at turn-on and at turn-off of the EMF, but in the sub-
sequent period only turn-off caused the described reaction. Finally, the con-
vulsive discharges disappeared.

Thus, in certain cases, the reaction to EMF turn-off can appear more sharp-
ly than the reaction to turn-on.

The stability of the reaction to turn-off is especially noticeable. For
example, the stability of this reaction and its latent period during exposure
to an SHF or a constant magnetic field did not change after sectioning the mid-
brain, although the corresponding indices of the basic reaction underwent signi-
ficant changes.

At the start of our description of the reaction to turn-off, we indicated
its nonspecific character; light, sound, an SHF field and a CMF caused similar
reactions. In this connection we should note that a similar reaction to turn-
off was noted after exposure to ionizing radiation [Grigor'yev, 1963]. In tests
on an isolated frog retina, it was shown that the reaction to turning on a light
carries information about the strength of the stimulus, and the reaction to turn-
off, about its duration [Val'tsev, 1964]. We feel that clarification of the
role of the reaction to turn-off is an interesting neurophysiological problem. /147
Certain general mechanisms for the reaction of excited structures to different
stimuli find expression in this event. More than likely, this reaction is re-
lated to the tracking processes.

Reactions at the Moments Electromagnetic Fields are Turned

On and Off

Certain investigators of the biological effect of EMF have noted reactions
to generator turn-off [Popov, 1940], but these were frequently reactions with a
short latent period. Consequently, the noted effects cannot be the reaction to
turn-off we have described above. Most likely, these were reactions similar to
the desynchronization reactions of the EEG that occur at the moment the genera-
tor is turned on and off. The reactions to turn-on and turn-off were studied in
greatest detail in the experiments on the effect of a CMF on the CNS, since

123

there we spoke about the possible induction of an electrical current in the
brain. However, this type of reaction occurred even more frequently during the
influence of UHF and SHF fi'plds on the rabbit head. For example, in experiments
on the effect of a pulsed SHF field with a power flux density of 10 mw/cm^, we
observed the desynchronization reaction at turn-on in 31% of the cases, and at
turn-off, in 12%. We should note that the corresponding indices at electromag-
net turn-on and turn-off were 7 and 19%.

Some part of the reactions at the moment of turn-on and turn-off can be ex-
plained by the effect of the clicks that appear when the generator is turned on
and off. But control tests with "false" switching, when there was a sound, but
no field, with silent switching of the generator and with deafened rabbits, show-
ed that the analyzed reactions occurred primarily during the appearance and dis-
appearance of EMF.

Are these reactions connected with the basic reaction and the reaction to
turn-off? We feel they are not, and this is why. The reactions to turn-on and
turn-off can appear independently of each other and of other reactions, which
does not indicate that the electrographic reactions during changes in the field
contradict the basic reaction and the reaction to turn-off. Furthermore, after
sectioning of the midbrain, the number of reactions occurring during changes in
the EMF decreases sharply, which can testify to their peripheral, reflex origin.
It is fully probable that these reactions are a result of an effect of EMF on
the receptors.

In their latent period (equal to a fraction of a second) and their electro-
graphic expression in the form of desynchronization, the reactions that occur
during a change in EMF are similar to the EEG reactions observed during the ap- /148
pearance and disappearance of such known stimuli as light, sound, an electrical
current, etc. The difference was that the desynchronization reaction during
change in the EMF occurred very rarely in comparison with similar reactions to
known stimuli.

Finishing our analysis of the EEG reactions that occur during change in the
EMF, we must say that these reactions are inherent to all the fields we studied,
both variable and constant. Such reactions can hardly be explained by an induc-
ed electrical effect since an ESF causes these reactions only at the beginning
of exposure and immediately after its cessation. The observed effects are prob-
ably nonspecific changes in the EEG that reflect the orienting reaction.

We can assume that reflex effects play a large role in the appearance of
the desynchronization reactions. The tests with an ESF showed that reactions
can occur at the moment of turn-on or turn-off when the basic reaction was ab-
solutely not observed, i.e., the mechanisms of the formation of these reactions
are probably different. We have already noted the possibility of different
mechanisms for the basic reaction and the reaction to turn-off. We can formally
separate three types of EEG reactions in response to the influence of EMF: 1)
desynchronization reactions, which occur at generator turn-on and turn-off and
which are evidently effected by one mechanism; 2) the basic reaction, which is
manifested as an increase in the number of spindles and slow waves during the
whole time of exposure; 3) the reaction to turn-off, which is externally similar
to the basic reaction, but differs from it in the mechanism of its appearance

124

and in quantitative characteristics. After this general survey of the EEG reac-
tions to EMF we can proceed to a more detailed characterization of the basic re-
action.

The Basic Reaction

The strengthening of spindle-shaped activity in the rabbit EEG that occurs
during exposure to EMF on the animal's head can also be recorded in the absence
of this influence. There are probably many other unconsidered stimuli that,
like EMF, can cause spindle-shaped activity on the EEG. Only localization of
the influence over time allows us to statistically separate the useful signal
from the noise level. Consequently, the unstable character of the reaction to
EMF can be explained not only by a variation of excitability, but also by the
effect of unconsidered stimuli on these structures, although more than likely
these two circumstances are two sides of the same process.

It is widely accepted that spindles are recorded on the EEG during sleep, /149
during light barbiturate anesthesia, in a classical isolated brain preparation
(cerveau isole) and during low-frequency electrical stimulation of the nonspecif-
ic formations of the thalamus. Although it is possible to observe a difference
in the form and frequency of spindles produced by different methods, for now we
shall speak of the general mechanism of their generation. It is probable that
the spindles that occurred in our experiments during exposure to an EMF on the
animal's head are also generated by this general mechanism [Gusel'nikov et al. ,
1963].

Spindles can be recorded in all sections of the brain down to the upper por-
tions of the spinal cord. When the subject changes to localization of the sec-
tion of the brain from which spindle-shaped activity is generated, some authors
point to the subcortical formations [Morison and Bassett, 1945; Kennard and Nims,
1949; Schneider et al., 1952; etc.], others to the cortex [Jouvet et al. , 1959;
Robiner, 1961; etc.], and others emphasize the necessity of thalamocortical con-
nections for the appearance of spindle-shaped activity [Okuma et al. , 1954;
Narikashvili, 1962; Serkov et al., 1960; etc.]. Since spindle-shaped activity
is frequently noted in the EEG of dogs upon restoration after clinical death,
there is an opinion that this activity is determined by the level of metabolism
in the brain cells [Gurvich, 1964].

Recent works indicate the appearance of spindle-shaped activity in response
to the influence of different stimuli. Thus, in response to an audio click
spindles have appeared in the auditory cortex, the medial geniculate body and
the reticular formation of the midbrain in cats drugged with curare [Kawamura
and Jamamoto, 1961].

During electrical stimulation of the radial nerve with square pulses with
a frequency of 5 Hz and a pulse length of 0.5 m/sec, the appearance of spindles
in the cortex was observed in an unanesthetized cat at a stimulation intensity
of 0.3 V, whereas changes in blood pressure and the appearance of muscle cur-
rents were not observed. At a stimulation intensity of 0.5 v, a desynchroniza-
tion reaction occurred immediately in the cortex, the blood pressure changed
and movements appeared. It is assumed that the reaction to weak electrical stim-
ulation occurs due to inhibition of the ascending activating system [Pompeiana,
1963].

125

Discussing the results of these works, Magoun (1963) noted that spindles
can be produced in the cortex during electrical stimulation of the pre-optic re-
gion or the caudate nucleus, and also after injection of glucose in the blood of
a hungry dog. The increase in synchronization and the number of spindles in the
EEG are sometimes connected with internal Pavlov inhibition since these electro-
pathic pictures occur during extinction of conditioned reflexes, and development
of differentiation and subsequent inhibition. Before the work of Pompeiana, the/150
appearance of spindles and slow waves was explained as stimulation of the non-
specific thalamocortical system, but now it has been shown that such a response
can be caused along specific paths. In conclusion, Magoun notes that the thala-
mocortical inhibiting system is in a reciprocal relationship with the reticular
ascending system.

The cooling of separate sections of the reticular formation with butane or
propane can produce spindles in the cortex of the Ips Hater al hemisphere that
disappear after stimulation by the gas has stopped [Naquet, 1963]. If the skull
is opened over one hemisphere of a cat that Is under f raxidlllc narcosis , spin-
dles appear in the opened hemisphere after several hours. These data support
the hypothesis that the cortex plays an active role in the formation of spindles
[Naquet, 1963].

Summing up this brief
discussion on the mechanism
of the appearance of spindles
on the EEG, we should note
that the appearance of spin-
dle-shaped activity is a re-
sult of complex intercenter
relationships, including spe-
cific and nonspecific systems.
The appearance of this form
of electrical brain activity
in response to external stim-
uli has been noted only in
several recently published
works. Our results of tests
with embedded electrodes sup-
port the hypothesis concern-
ing the participation of many
brain structures in the for-
mation of spindle-shaped ac-
tivity.

The coiq)aratlve results /151
of EEG reactions to a CMF,
light and sound of average
intensity are given in Figure
53. In her tests, S. N.
;-mlnute exposures to CMF, light

and slow waves indicate that
e the nimiber of these EEG in-
numb er.

i fi 25 35 V5 5S

JJ Vf 55

Figure 53. Dynamics of the Number of Spindles
in the Electrical Activity of the Rabbit Cor-
tex in the Background and During Exposure to
a CMF (1), Light (2) or Sound (3). A = Before
Exposure; B = in the Influence Period; C = in
the Aftereffect Period. Key: (a) Number of
Spindles and Slow Waves, %; (b) Time, Sec.

Luk'yanova alternately gave the same rabbit 40 one
and sound. The dynamics of the number of spindles
during the exposure, light and sound sharply reduc
dices, but a CMF has a tendency to increase their

126

However, when R. A. Chizhenkova exposed a rabbit for one minute to a sound
with an intensity on the human audible threshold, there was an increase in the
number of spindles on the EEG, although the dynamics of these changes differed
from the corresponding index for a CMF. Certain reference data [Pompeiana, 1963]
and the experiments of Chizhenkova indicate that the primary desynchronization
reaction cannot be observed on the EEG in response to a weak stimulus, but rath-
er an increase in the number of spindles and slow waves. We should probably
speak of the general regularities of a 3-phase effect of stimuli (weak, average,
strong) in addition to the known 2-phase effect (average, strong), which has
found expression in the Arndt-Schultz law. This question has already been dis-
cussed in the references [Simonov, 1962], and the study of the effect of EMF on
the CNS can render a large contribution to resolving the general biological prob-
lem of the phase nature of the influence of stimuli.

To conclude our discussion of the basic EEG reaction occurring during expo-
sure to an EMF, we should say several words about its latent period. In all ex-
periments involving UHF, SHF and constant magnetic fields, the latent period of
the EEG reaction varied from 1 to 100 sec. We have already discussed the possi-
ble physiological significance of one or another peak on the latent period dis-
tribution curve. Here, we only want to say that, changing within certain limits,
depending on the functional state, the intensity, and other physical characteris-
tics of the EMF, the latent period remains long. We have the impression that
the EEG reaction to EMF is effected by some slow regulatory system. It is pos-
sible that this slow system is analogous to the one indicated in the investiga-
tions of N. A. Aladzhalova (1962) and certain American authors [Becker et al. ,
1962].

The Direct Effect on the Brain

We consider the most important fact in our investigations to be the clari-
fication of the presence of a direct effect of EMF on isolated structures of the
brain. Until now, proof of the direct effect of EMF on the CNS was seen in the
more intensive reaction during local exposure on the animal's head, in the lead-
ing role of the exposed hemisphere (during a unilateral exposure) , in the devel- /152
opment of functional changes, and in the predominance of effects on the contra-
lateral side of the body with respect to the exposed hemisphere [Bychkov, 1962;
and others].

Experiments on an isolated brain preparation and a neuronally-isolated cor-
tical strip showed that SHF, UHF and CMF have a direct effect on the brain tissue.
Furthermore, this effect was more ably revealed in the isolated sections than in
the intact brain.

The. very fact of a direct effect of certain stimuli on the CNS has been no-
ted by many investigators. The effect of an electrical current and certain chem-
ical substances is a common fact. "Compounds circulating in the blood stimulate
primarily the chemoreceptors of tissue and vessels (and possibly also formations
similar to chemoreceptors in the brain tissue)."* R. Granit (1957) indicated the
possibility of certain receptors being doubled in the brain to improve the regu-
latory system.

'^. M. Bykov: Isbv. proizvedeniya. (Selected Works.) Volume 1, 1953, p. 6-7.

127

By directly affecting the basic link of the regulatory processes, we can
control the activity of the entire organism. The importance of a direct effect
of a stimulus on the CSS is indisputable; we can speak only of the propagation
of this phenomenon, of its normal or pathological character. Speaking of its
propagation, we must consider both organisms with different levels of biological
organization and factors of a different physical nature. It is known that one-
celled animals perceive any stimulus directly with their one cell. Only after a
significant period of development do multicellular organisms acquire sensory
organs. In the vertebrate lancelets, for example, light is still perceived by
the brain itself.

Thus, the form of the interaction of an organism with the medium in which
we are interested is an old method and the only method of interaction in the low-
er stages of evolutionary development. Each living cell of a complex organism,
regardless of its high degree of specialization, retains the ability, to some
degree, to react directly to separate factors of the environment. Finally, among
the cells of different systems, the nerve cell occupies one of the foremost po-
sitions in this respect.

Even such stimuli as light and sound, perceived by complexly organized
special receptors, also act directly on the CNS. Thus, light acts on the dien-
cephalon of fish [Frisch, 1911; Scharrer, 1928], amphibians [Dodt, 1963] and
birds [Benoit, 1955], and sound acts directly on the spinal cord [Nasonov and
Ravdonik, 1950]. Therefore, it is not surprising that stimuli for which there /153
are no specialized receptors can be perceived by such an "ancient" method, i.e.,
directly on the CNS.

In the future, when there is an increase in the intensity or the duration
of the effect, EMF can also affect other systems and organs. Therefore, for a
full description of the process of interaction of an organism with the medium,
we must consider all the possible effects on the receptors, the nervous system
and other systems of the organism.

We have limited our problem to a study of the effect of EMF on the CNS.
But even this fragment of the complex process of interaction of an organism with
the medium is not uniform. We have already noted that the structures of the hy-
pothalamus and cortex are most reactive to the influence of CMF. It is perti-
nent to recall that many physiotherapists [Shcherbak, 1936; Slavskiy, 1937; and
others] have noted the special sensitivity of the higher autonomic centers in
the diencephalon to physical factors.

There are electrophysiological investigations of the effect of diathermy
procedures. Simultaneous recording of the electrical activity of the cortex and
the diencephalon has been conducted. In their analysis of the site of diathermy
application, the authors state: "It is possible that bitemporal diathermy
causes changes in the higher autonomic centers of the hypothalamus region. The
fact that all the observed changes are most significant and constant in the di-
encephalon, and that changes in the cerebral cortex either follow behind those
in the diencephalon, or generally do not exist, also allows us to consider the
changes in the diencephalon after bitemporal diathermy as primary. The changes
in the cerebral cortex are the result of secondary effects of diathermy through

128

the centers of the dlencephalon.

II*

As our experiments with a CMF have shown, the presence of the most inten-
sive reaction of the diencephalon still cannot be considered direct proof of the
priority of just this section of the brain in the reaction. We can assume that
the changes in electrical activity occur synchronously in all sections of the
brain, that the changes in the diencephalon are of secondary origin, but more
intensive, etc. However, the certain similarity in the electrical reaction of
the brain to EMF and high-frequency currents allows us to assume that the reac-
tions we have studied are nonspecific.

As for the question regarding the precedence of the appearance of an elec-
trical reaction in the cortex or the diencephalon, we should note that an iso-
lated cortex can react to EMF. We shall try to clarify the role of the dien-
cephalon m experiments on fish, in which the cortex is lacking.

In concluding our discussion of the results obtained by means of electro- /154
graphic methods, we want to note that the methods we applied are rather artifi-
cial, are necessarily connected with considerable intervention in the behavior
of animals, and are frequently just acute tests (isolation of different sections
of the brain) . The unavoidable harnessing of the animal created additional
stimulation, not to speak of the embedded electrodes, which, by themselves, induc-
ed definite trauma and also necessitated the removal of the scalp from the upper
surface of the skull.

But, like any other method, electrography also has its advantages. Only by
means of this method could we show the direct effect of EMF on many structures
of the brain, establish the participation of neurons in the reaction to EMF, and
distinguish the reaction to turn-off. We look for further study on the physio-
logical effect of EMF on the CNS: on the one hand, in investigations of the mor-
phological changes caused by EMF in the brain; and on the other hand, in an anal-
ysis of the physiological reactions of an intact organism to EMF by the method
of conditioned reflexes, as well as by other methods.

Blagovidova et al. : Byull. Eksperiment. Biol, i Med. ^(5): 12, 1962.

129

PART II. THE CONDITIONED REFLEX /155

METHOD OF STUDYING THE EFFECT
OF ELECTROMAGNETIC FIELDS ON
THE CENTRAL NERVOUS SYSTEM

We would like to begin this section of our investigation with a statement
by the well-known Soviet physiologist, L. A. Orbeli. "Until now, we have experi-
mentally studied the effect of these ultrahigh frequencies from a narrow practi-
cal point of view: their application in technology, their use in medicine, etc.;
but now they have become one of the possible factors in the evolutionary pro-
cess. We must therefore study ultrasonic frequencies, electromagnetic waves of
different frequencies, and ultraviolet light not only from the point of view of
their effect on individual functions and individual organisms, but we must also
study them from the point of view of their possible role in the evolutionary
process, and the role that they have played in the evolutionary process, there-
by clarifying how they can affect future generations."*

The evolutionary approach to the study of the biological effect of electro-
magnetic fields has just begun. There are now fragmentary reports concerning
the participation of EMF in the basic processes of the origin of life [Miller,
1955] , the effect of EMF on the formation of biological rhythms [Brown, 1962] ,
the ontogenic development of the organism [Barnothy, 1960] , the effect of EMF
on the higher invertebrates [Brown et al. , 1960], and the orienting effect of a
CMF on migrating birds and fish [Yeagley, 1947, 1951]. In the light of the
fragmentary data on the effect of natural-state EMF on the functions of the en-
tire organism, we set before ourselves the basic task of studying the effects
of EMF on the most sensitive index of the activity of an animal, i.e., on its be-
havior. As an adequate method of investigation, we selected the Pavlov method
of conditioned reflexes, which has been successfully used in studying the prop-
erties of many stimuli which act on exteroceptors and interoceptors, as well as
directly on the CNS.

In studying the biological effect of EMF, the effect of different fields on/156
pre-developed positive and negative conditioned reflexes to light and sound has
most frequently been investigated. Detailed reports on such studies are given
in the beginning of the first chapters of this part of the book. It seemed to
us that the most complete data on the physiological effect of any factor can be
obtained by using it as a conditioned stimulus.

This type of work has been carried out on man during the development of a
conditioned electrodefensive reflex to low-frequency EMF [Petrov, 1952], on fish
during the development of motor (feeding) conditioned reflexes to a CMF [Lissman,
1958] and on white mice in the development of electrodefensive conditioned re-
flexes to an SHF field [Malakhov et al. , 1963]. In all these cases, the authors
managed to develop a conditioned reflex to EMF, but it was distinguished by its
late appearance and instability.

*L. A. Orbeli: Izbrannyye trudy. (Selected Works.) Volume 1. Moscow and
Leningrad, Izd-vo AN SSSR, 1961, p. 65.

130

EXPERIMENTAL METHODOLOGY AND TREATMENT OF RESULTS

The tests were conducted on rabbits, pigeons and fish (carp, goldfish,
stickleback, flounder and bullhead). Rabbits were used in order to compare the
results obtained by the electrographic and conditioned reflex methods, and pig-
eons and fish were selected because of their ability to orient themselves along
the earth s magnetic field during long migrations. This explains why we used a
CMF m most tests. Besides a CMF, we studied the effect of a UHF field and v-
radiation only in the experiments with fish, on which the essential part of the
investigations was carried out.

Procedure for Development of Conditioned Reflexes

Considering the results of electrophysiological tests that proved the pres-
ence of the biological effect of a CMF, we tried to develop conditioned def ens-
^Ico^ "^^^ ^" rabbits by the shaklng-off method [Bolokhov and Obraztsova,
1958], using a 300-Oe CMF. Numerous experiments that involved recording the
rabbit EEC have shown that a CMF does not cause unconditioned motor reflexes in
animals. The sound stimulation (a 500-Hz tone produced by a ZG-10 generator)
used as a control also did not cause movements. Unconditioned electrical stim-
ulation from the ZG-10 generator was applied to the rabbit's ear. During the
tests, the animal was harnessed to a stand just as in the electrophysiological
experiments. Its head was placed between the poles of an electromagnet. Head /157

movements were recorded on a kymograph with the aid of an electrical contact,

the closing of which caused the movement of an ink stylus.

The isolated effect of the conditioned stimulus lasted 7 sec, and its joint
effect with the unconditioned stimulus lasted 3 sec. During a test, we admin-
istered 10 combinations with an interval of 5-10 min. A total of 14 tests were
conducted on an individual rabbit, so that each received 70 exposures to sound
and magnetic stimuli.

In the tests on birds and fish, we used the food-getting method developed
in the laboratory of Professor L. G. Voronin. The essence of this method is
that before the animal received food reinforcement, it had to make a special
food-getting motion during the influence of the conditioned stimulus: in partic-
ular, the pigeon had to peck at a handle. The fundamental description of this
method is given in the works of L. G. Voronin (1953, 1954, 1957); the specific
method for fish, in the works of N. V. Prazdnikova (1953); and for birds, in the
work of A. V. Baru (1953).

The diagram of the apparatus for developing conditioned food-getting re-
flexes in pigeons is given in Figure 54. During the influence of the condi-
tioned stimulus, the pigeon pecks at a handle, thereby closing an electrical
contact, or pecks at a tin plate fastened to a Marreyev capsule, and this con-
ditioned reflex movement is noted on the kymograph drum via air transmission
through another Marreyev capsule. The conditioned stimuli were light from a
40-w electric lamp, a gurgling sound, and a 200-Oe CMF created by a solenoid
wrapped around the chamber. Most frequently the food reinforcement was hemp

131

seeds. The exposure time to the conditioned stimulus was 20 sec. Reinforce-
ment was given Immediately after the conditioned reaction was accomplished.
The conditioned stimuli were applied in intervals of 1-3 min. In a test we ap-/158
plied the conditioned stimuli 10-20 times. To develop conditioned inhibition
after the conditioned food-getting reflexes had been developed without a stereo-
type, the pigeons were exposed to a combined stimulus (a CMF + light) 2-3 times
during a test; for this the solenoid was turned on 10 sec before the light was
turned on, but both stimuli were turned off simultaneously. The combined stimu-
lus was not accompanied by food reinforcement.

Figure 54. Diagram of Apparatus for
Developing Food-Getting Conditioned Re-
flexes in Pigeons.

The diagram for developing condi-
tioned food-getting reflexes in fish
is shown in Figure 55, A. By pulling
the bead in response to the condi- /159
tioned stimulus, the fish closes an
electrical contact, which is record-
ed on the kymograph drum with the aid
of an electromagnetic marker. The
conditioned stimuli that we used were
the light from a 50-w electric lamp
placed over the aquarivrai, the sound
of an electric bell attached to the
wall of the aquarium, and a CMF. The
CMF was created by a permanent magnet
and also by switching on an electro-
magnet or a solenoid.

The diagram of the apparatus for developing the electrodefensive condi-
tioned reflex is shown in Figure 55, B. As in the method of J. P. Frolov
(1925), the conditioned reflex response is the general motion of the fish re-
corded on the kymograph drum with the aid of two Marreyev capsules. To do this,
one end of a thread was attached to the dorsal fin of the fish and the other
end was fastened to the handle of one Marreyev capsule. The conditioned stimuli
were the light of a 50-w bulb, the sound of an electric bell, and the variable
50-Hz magnetic field of the solenoid wound around the aquarium. The exposure
to the conditioned stimulus was noted by an electromagnetic marker on the sec-
ond (from the top) line of the kymograph. Unconditioned electrical reinforce-
ment was applied through electrodes immersed in the aquarium; current was fed
to them from the secondary winding of the induction coil. The moment the uncon-
ditioned reinforcement was turned on was noted on the kymograph by means of an
electromagnetic recording on the third line from the top. As in the tests em-
ploying the food-getting method, the time was noted on the bottom line of the
kymograph in 5-sec intervals.

Not all the fish were easily secured with a thread to the handle of the
Marreyev capsule. Therefore, in tests with flounder and bullhead, we did not
record the conditioned reflex movements of the fish on the kymograph.

The movements of the fish were also recorded with a probe floating on the
water; the needle transducer that transformed mechanical oscillations into

132

^— ^^^^IBBSJH

Figure 55. Diagrams of Apparatus for Developing Condi-
tioned Reflexes in Fish by the Food-Getting (A), Electro-
defensive (B) and Defensive (C) Methods.

electrical oscillations was attached to it. As a result of the motion of a fish
the water began to oscillate, and the probe with it, which caused the transducer
to move. An electrical signal was fed from the transducer to the input of an
amplifier, and then to a recording oscillograph. The feeding of conditioned and
unconditioned stimuli were automatically recorded. The conditioned stimulus for

this method was total-body Co gamma-irradation in a dose of 0.5-0.1 R/sec (des-
ignated below as irradiation) and a 500 v/m UHF field created by a UVCh-40 (UHF-
40) generator.

We also used another defensive method, the diagram of which is given in
Figure 55, C. In this method, the conditioned reflex movement is the swinmiing of
the fish from one half of the aquarium to the other. The fish swam through an
opening in a partition that divided the aquarium into two equal parts. The /160
conditioned stimulus was the light of a 50-w electric lamp; one light was placed

133

over each half of the aquarium. The coaditioned stimulus consisted of turning
off the light in the half of the aquarium where the fish was at the given moment
and turning on the light in the opposite half. A CM? created aeat uue partition
by turning on an electromagnet or a solenoid, or by setting up a permanent mag-
net also served as the conditioned stimulus. The unconditioned reinforcement
consisted of mechanical stimulation with a finger or with air bubbles passed
through a plastic hose.

In the development of a positive conditioned reflex by the food-getting me-
thod, the conditioned stimulus lasted 30-40 sec, and by the defensive ^aethod.
10-15 sec In the latter case unconditioned reinforcement was given for 7-10
sec only if there was no conditioned response. In the development of conditioned
inhibition, the magnetic field was created 10 sec before the positive conditioned
stimulus was given, and it was turned off together with this ^^Imulus. Tests
were conducted once or twice a day. Conditioned stimuli were applied 10-20 times
in each test, of which 1-5 inhibiting stimuli were applied without a stereotype.
All the tests were recorded. The lag period was determined by a stop watch with
an accuracy to 0.1 sec.

Surgical Methodology

To determine the physiological mechanism of EMF perception in fish, we per-
formed denervation of the lateral line organ, enucleation and destruction of
different sections of the brain. We compared the results on the development of
conditioned reflexes in intact and operated fish. In certain tests the opera-
tion was conducted after preliminary development of the conditioned reflexes.

Denervation of the lateral line organ was performed by bilateral section of
the n. lateralis and n. suborbitalis according to the method described by G. A.
Malyukina (1955). The fish was taken from the water and wrapped in a wet clotn.
The nerves were dissected and transected. When the fish was put ^^^/^^^^
aquarium it did not differ in external behavior from intact fish. The described
method did not achieve full denervation of the lateral line organ and our prob-
lem was to clarify the question of how partial denervation of the lateral line
organ affects the conditioned reflex activity of fish.

Enucleation was accomplished by sectioning the optic nerve and '^o'^letely
removing the eyeball from the orbit. Immediately after the operation the fish
developed motor restlessness and ran into the walls of the aquarium However,
after several days the external behavior of the blinded fish did not differ /Ibl
from the behavior of intact fish, although they could always be distinguished
by the darker coloring of their skin surface.

The operations to remove different sections of the brain were inducted by
the method described by A. I. Karamyan (1949). A fish was taken from the aquar-
ium and wrapped in a damp cloth in such a way that its head remained pee. At
ter 1-3 min in air the fish ceased to move. Holding the fish in the left hand,
with scissors in the right, we made a transverse section of the sfcuii Done oe
hind the line connecting the posterior edges of the eyes. Then we made two
parallel longitudinal incisions back from the edges of the transverse section.

134

The piece of bone was raised, but left attached to the rear side; the fatty tls^
sue was removed with cotton swabs and the brain was exposed.

The brain of a goldfish (Figure 56) is divided by sharp boundaries into
hJtln''''*^ '"'■f ^k"" i^^^T^ tegmenta) and the cerebellum. The forebraln could
be removed and the visual tegmenta damaged with sufficient ease because of their
surface position. Complete removal of the cerebellum was complicated by the
dSr^nf /'^ ^ti^'u ^^ l^'^^ted under the visual tegmenta. Destruction of the
diencephalon, which composes the basic part of the brain under the visual teg-
menta was a more complex operation. We destroyed the anterior part of the di-
encephalon after removal of the forebraln.

Figure 56. Diagram of the Structure of
the Goldfish Brain. 1 = Forebraln; 2 =
Diencephalon; 3 = Midbrain; 4 = Cere-
bellum; I = Top View; II = Side View.

The results of the operations /162
to remove the cerebellum were con-
trolled very strictly because the
main role in accomplishing the fix-
ing functions in fish is ascribed
to this section of the brain [Kara-
myan, 1949; Malyuklna, 1955]. The
results of operations to remove the
forebraln and the visual tegmenta
were checked visually on an intact
brain. The results of operations to
remove the cerebellum were checked
on a series of brain sections of
dead fish. Removal of the cerebellum
was complete. The morphological work
was conducted in the CNS Morphology
Section of the Department of the
Physiology of Higher Nervous Activity
at Moscow State University under the
direction of Professor G. I. Polya-
kov and his research associate, V.
M. Svetukhina.

„ . . r. , , After removal of the necessary

section of the brain, the opening in the skull was covered with the bone and
smeared with a mixture of beeswax and vaseline (in a 2:3 ratio). We evaluated
the changes caused by the extirpations from the character of the movements of
tne fish immediately after the operation, from how they related to food, but
basically from the results of the development of conditioned reflexes to a mag-
netic field, and to light and sound. The rate of formation and the stability
of the conditioned reflexes after extirpation of separate sections of the brain
were compared with the corresponding indices for Intact fish.

During destruction of the midbrain, each visual tegmentum was peeled off
the brain and cut along the edges with an eye knife. For several hours iiraned-
lately after the operation the fish lay immobile on the surface of the water.
Some of them were in a contorted position. The fish swam in circles, rolled
from side to side, accelerated rapidly and slowed down, and sometimes they
turned around the longitudinal axis of the body. These disturbances in

135

movements are probably related to primary or secondary damage to the cerebellum.
M^er the operation the fish did not actively take food, but they would swallow
insects placed in their mouths.

To remove the valve of the cerebellum, the visual tegmenta were moved
slightly the valve was freed from under them, raised upwards, and cut from the
body of the cerebellum by a vertical section. The body of the cerebellum was
separated from the remaining brain by a horizontal section. For some txme im-
mediately after the operation the fish lay on their sides on the surface of the
water. The first motions of the fish were uncoordinated, i.e., they swam in tne
lateral position, and made circles and corkscrew movements. However, after
several days some fish assumed their normal body position and began to swim,
rolling slightly. The operated fish did not begin to actively take food for
two weeks after the operation. In the same period of time we managed to develop
relatively stable conditioned reflexes in them, although the appearance of con-
ditioned reflexes could also be observed sooner.

The operation of removing the forebrain is simple and easily withstood by /163
fish. After opening the skull, we separated the large hemispheres from the re-
maining brain mass with a vertical section and removed them from the skull.
After the operation the fish immediately assumed their normal position, moved
about normally, and began to actively take food after several minutes. Condi-
tioned reflexes could be developed immediately after the operation, although we
usually started to develop them after several days.

We destroyed the diencephalon after preliminary removal of the forebrain
by a section backwards and downwards into the base of the brain under the vis-
ual tegmenta. In doing this, we basically destroyed the hypothalamic part of
the diencephalon [Kappers et al., 1936; Zelikin, 1957]. The behavior of the
fish after the operation was the same as after the removal of the forebrain,
although in certain fish we observed brief disturbances in movements, and these
fish began to actively take food later. The vision of certain fish was des-
troyed and their coloring became darker.

Methods of Treating the Results

A conditioned reflex is primarily characterized by its rate of formation.
Here we consider the rate of its appearance, i.e., the number of applications
of the conditioned stimulus necessary for the first performance of the retiex,
and the rate of its fixation, i.e., the number of applications of thecondi-
tioned stimulus after which the conditioned reflex appears five times in a row.
We calculated the stability of the conditioned reflex, i.e., the percentage
ratio of the number of applications of the conditioned stimulus that caused a

conditioned response to the total number of applications of the ^^i?|"^^^','j5'itv
in one test and also in 50 and 100 applications of the stimulus. The stability
of the conditioned reflex in the first 50 or 100 applications of the condition-
ed stimulus was used as an index in comparing the conditioned reflexes to one
stimulus in different animals and the reflexes to different stimuli in the same
animal. In calculating the lag period of the conditioned response we ^suaily
counted only the applications of the conditioned stimulus that caused a response.

136

The average of the lag period was calculated within one test, and in 50 or 100
applications of the stimulus.

Although intersignal reactions were observed in all experimental animals
in the development of conditioned reflexes by different methods, we analyzed
the intersignal reactions only in tests according to the food-getting method.
Intersignal reactions in the form of intermediate salivation ware noted even in
the first works of the Pavlov school; however, they became the object of a spe-
cial study comparatively recently [Voronin, 1954; Skipin, 1947; Shirkova, 1956;
Meshcherskiy, 1957; and others]. It has been noted that the number of inters ig- /164
nal reactions depends not only on the type of nervous system, the character and
degree of the unconditioned stimulus, but also on the conditioned stimulus.
This fact forces us to analyze the dynamics of intersignal reactions with parti-
cular care in order to clarify the properties of the factor used as the condi-
tioned stimulus with respect to all indices.

The listed indices of conditioned reflex activity were tabulated and ex-
pressed graphically. The test results were sometimes illustrated by kymograms
or test records.

137

CHAPTER 5. THE DEVELOPMENT OF CONDITIONED REFLEXES TO ELECTRO-
MAGNETIC FIELDS IN RABBITS, PIGEONS AND FISH

Although representatives of the mammals, particularly the dog [Pavlov, 1927,
1951], served as the classical subject in the study of higher nervous activity,
the basic regularities of behavior are common for mammals, birds and fish [Bay-
andurov, 1937; Frolov, 1941; Voronin, 1957; Thorpe, 1956; Bull, 1957]. The me-
thod of conditioned reflexes has been used especially widely to determine the
receptor possibilities of different species of animals.

The Development of Conditioned Reflexes to a.
Constant Magnetic Field in Rabbits

Figure 57 shows the results of experiments on the development of condition-
ed shaking-off reflexes to sound and CMF in one rabbit. We can see that in the
first 4 tests, when intersignal reactions abounded, certain movements could co-
incide with the influence of the CMF 1-2 times per test. Later, the conditioned
reflexes to sound tended to increase, and a reaction of the rabbit did not occur
in response to turning on the electromagnet. These tests were conducted by R.
A. Chizhenkova on 3 rabbits. Not one of the animals developed a conditioned
reflex to a CMF.

Although we cannot now make a
final conclusion about the possibility/165_
of developing conditioned reflexes to
a CMF in rabbits, since only 70 com-
binations were given and the strength
of the CMF was low, the test results
testify to the weaknesses of a CMF as
a stimulus in comparison with sound.

(a) /

/ \

/ ^^

1-^

/ \

■<< 1

\ 1

1 \

' \

-4

1/ S 6 7 9 H
(b) ffOMCp onmma

10 fi n 13 It

Figure 57. Dynamics of the Development
of Conditioned Defensive Reflexes in
Rabbits to a Magnetic Field (1) and
to Sound (2). Key: (a) Number of
Reactions per Test; (b) Test
Number.

Since we frequently observed EEG
changes after a 7-second exposure to
a CMF, it is possible that the dura-
tion of the isolated influence of the
CMF as the conditioned stimulus was
insufficient. However, increasing
the duration would introduce addition-
al complications into the process of
developing a conditioned reflex.
Changing to the feeding method would
require huge new sources of CMF.
Therefore, we decided to conduct ex-
periments with conditioned reflexes
on smaller animals, representatives
of other classes of vertebrates.

138

The Development of Conditioned Reflexes to a
Constant Magnetic Field in Pigeons

We tried to develop a positive conditioned reflex to a CMF and conditioned
inhibition to a combination of stimuli (CMF + light) in pigeons. The tests were
conducted according to the food-getting method.

The Development of a Positive Reflex to a Constant
Magnetic Field

The results of a series of experiments conducted on 4 pigeons showed that
a positive conditioned reflex to a CMF could not be developed in pigeons, al-
though each bird was given from 190 to 427 combinations. The conditioned re- /166
flex to light appeared in the same pigeons after 7-16 combinations and became
fixed after 18-26 combinations.

In calculating the number of Interslgnal reactions we found that when
light was used as the conditioned stimulus 41, 135 and 52 interslgnal reactions
arose in 3 pigeons for 100 applications, but when a CMF was used, 133, 208 and
128 Interslgnal reactions arose. This fact cannot be explained by a difference
in food excitability since both the CMF and light were reinforced with food.
The excess of interslgnal reactions also cannot be explained by an orienting re-
action to a new stimulus because in pigeon no. 1, for example, during 400 appli-
cations of the stimulus the number of Interslgnal reactions during the develop-
ment of a conditioned reflex to a CMF was approximately 3 times greater than
the number of Interslgnal reactions during development of a light conditioned
reflex. We can conclude that the physiological effect of a CMF is revealed by
the increase in the number of interslgnal reactions. However, the cases of in-
terslgnal reactions we considered occurred in the intervals between exposures
and can be explained only by the aftereffect.

We felt it was important to check whether the Increase in the number of
interslgnal reactions occurred during the application of the CMF. We managed
to do this on 2 pigeons (no . 3 and 4) in a series of experiments in which a
positive conditioned reflex to light was developed in 10 tests, and in 10 other
tests we switched on the solenoid for 10 seconds before the start of the light
effect and switched it off together with the light. The tests in which the CMF
was used were Irregularly mixed with tests in which only the manipulations ac-
companying switching of the solenoid were performed, but current was not fed to
the solenoid. Each application of light with or without a CMF was reinforced
with food. We calculated the total nvraiber of interslgnal reactions per test
and the number of Interslgnal reactions for the 10 seconds before the light was
turned on, i.e., for the period of time when the CMF operated Independently.
The test results are given in Table 17.

In some pigeons the number of interslgnal reactions for the whole test
with the Influence of a CMF exceeded the number of interslgnal reactions for
the whole test without the influence of the magnet. However, this excess be-
comes insignificant or completely disappears if we subtract the total number of
Interslgnal reactions for the 10 sec before the light was turned on from the

139

TABLE 17. NUMBER OF INTERSIGNAL REACTIONS IN PIGEONS
nURING THE INFLUENCE OF A CMF AND IN ITS ABSENCE.

/1$7

For the 10 sec before the light

For the whole test

Test
Number

was turned on

pigeon no. 3

pigeon no. 4

pigeon no. 3

pigeon no. 4

with

without

with

without

with

without

with

without

the

CMF

Total

135

271

175

total number of intersignal reactions for all 10 tests. For pigeon no. 3 this
difference is (271 - 135) - (175 - 47) = 136 - 128 = 8 intersignal reactions,
and for pigeon no. 4 (38 - 23) - (31 - 11) = 15 - 20 = -5 intersignal reactions.

Consequently, the difference in the number of intersignal reactions in the
test and in the control basically appears during the influence of the CMF. Ac-
tually, in pigeon no. 3 the number of intersignal reactions during the influence
of the magnet was approximately 3 times greater (135 vs. 47), and for pigeon no.
4, approximately 2 times greater (23 vs. 11) than in similar periods of time in
the tests without the application of the magnet. This series of tests shows
that during the influence of a CMF the number of intersignal reactions increases
significantly. In the intervals between applications of the magnet we do not
find an increase in the number of intersignal reactions and we explain this by
the fact that the magnet acts together with the light in this case.

Finishing our discussion of the question concerning the effect of a CMF on
the number of intersignal reactions, we must note that a CMF increases the num-
ber of intersignal reactions both during its influence and in the intervals be-
tween applications of the stimulus, when it is applied without the light.

140

Consequently, the physiological effect of a CMF also appears after the end of
its physical action.

The Development of Conditioned Inhibition to a
CMF + Light Stimulus

The lack of success in developing positive conditioned reflexes to a CMF in
pigeons forced us to try to develop conditioned inhibition to this factor. The
tests were conducted on 3 pigeons. We applied a combined CMF + light stimulus
after fixation of the light-conditioned reflex.

In pigeon no. 2 the CMF caused an inhibiting effect in the first applica-
tion.

In pigeons no. 1 and 3 the inhibiting effect of a CMF appeared on the 5th
application. As an illustration we give the kymograms of the tests, on which
one can clearly see the presence of the inhibiting effect of a CMF (Figure 58).

"* ' 7^) (i) {a3 [bl Ca5 /168

ce . cs_ . a Ma *c6 cb

. ^ .

302 303 30V n 305

1 l llllllllllll l l l i n illll lll llllllll ll lll l ll l l l l HIIHII II IIII |iii | !l! lii l ll lll|l|| |

(a) (a) (a) (b) (a) (a) (a)
C B CB Cd 1a *CB CB C b CB

138 139 IV0 5 'VI m m

Figure 58. The Inhibiting Effect of a Constant Magnetic

Field on a Light Conditioned Food-Getting Reflex. A =
Pigeon No. 2; B = Pigeon No. 1; 1 = Marking of the Food-
Getting Motion; 2 = Marking of the Effect of the Conditioned
Stimulus; 3 = Time Marking with a 5-Second Interval; MF =
Magnetic Field; L = Light; the Numbers Indicate the Number
of the Stimulus Application. Key: (a) L; (b) MF + L.

We should note that the effect of a CMF is sometimes exhibited not only in
the time when the solenoid is turned on, but also during the next application
of the light stimulus. This sequential inhibition strongly varied in intensity.
Most frequently, it increased the lag period for the light stimulus following
the CMF. The stimulus following the conditioning-inhibiting stimulus often
did not cause a conditioned response. In pigeon no. 2 the CMF applied in the
fourth position in the test inhibited not only the response to the light given
together with the CMF, but also the reaction to the next 6 light stimuli. The

141

pigeon stopped pecking at food given to it, and tried to break out of the cage.
Sometimes a negative behavior reaction to a CMF appeared: t..e pigeon walked
away from the feeder, although usually, even in the intervals between stimuli,
the pigeon stood near the feeder.

All the cases of inhibition are summarized in Table 18. The tabular data
show that, although sequential inhibition was observed most frequently in pigeon
no. 1, it appeared most intensively in pigeon no. 2, for which a third of all
the cases of sequential inhibition are made up of full inhibition of more than ^^^^

2 responses to light. In pigeon no. 3 sequential inhibition appeared less

marked in both frequency and intensity than in the two other pigeons.

It is evident that in 9-19% of the cases the effect of a CMF appears only
in the form of sequential inhibition. Cases of immediate inhibition are more
frequent. The stability of the inhibiting reaction is essentially reflected in
the last column of Table 18, where all the cases of the inhibiting effect of the
magnet are totaled. We see that in pigeon no. 1 the magnet failed to cause an
inhibiting effect in only 6 cases out of 70. In^ pigeons no. 1 and no. 2 the
stability of the inhibiting reaction attained 60%.

In discussing the data obtained, we wondered whether the procedure of turn-
ing on the solenoid and the sounds that arise during this may cause the immedi-
ate inhibition, and whether the absence of food reinforcement under the in-
fluence of the magnet is the cause of sequential inhibition. To check these
questions we conducted control tests on pigeon no. 2, for which sequential in
hibition was most intensive. There were 35 tests in the series: 17 continued
the development of conditioned inhibition in the normal manner, but in 18 tests
the procedure of switching on the magnet differed in that there was no electri-
cal current in the solenoid. During the pretended switching, as during the
actual, food reinforcement was not given. During this series of tests 50 appli
cations of the CMF + light stimulus and 50 false switchings plus light were
given. Tests with the influence of the magnetic field were randomly mixed with
tests accompanied by false switching of the solenoid. Table 19 gives the com-
parative characteristics of inhibition observed during the influence of the mag
net and in the control tests.

The total number of cases of sequential inhibition under the effect of the
magnet exceeded the number of cases observed during false switching by 3.5
times (28 vs. 8%) (p < 0.05). Consequently, our assumption that the frequent
displays of sequential inhibition with the CMF + light stimulus are caused by
the properties of the magnetic field, and not by the absence of food reinforce-
ment of the preceding light stimulus, is valid.

Sequential inhibition after application of a CMF was not only observed
more frequently, but it appeared with greater intensity. Thus, we see that in
almost half (6 out of 14) of the cases of sequential inhibition after the in-
fluence of a CMF we observed full inhibition of one or several conditioned re- /171
sponses to light. After the procedure of false switching, sequential inhibition
was most frequently expressed in an increase of the lag period of the condition-
ed reaction to the following stimulus.

142

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143

The ancillary stimuli that arise when the solenoid is turned on may cause
inhibition of the response to the light that is applied together with the magnet,
but, as Table 19 shows, the conditioned light reflex is inhibited 6 times more
frequently during the influence of the magnet than during false switching
(p < 0.05).

We can consider that inhibition of the conditioned reflex to light is the
clearest physiological property of a magnetic field. Sequential inhibition also
characterizes the physiological effect of a magnetic field, although to a lesser
degree. Sometimes the CMF caused only immediate inhibition, more frequently it
caused immediate and sequential inhibition, and sometimes it caused only sequen-
tial inhibition. The most complete characterization of the inhibiting effect of
a CMF is a total of the cases of immediate and just sequential inhibition, which
is reflected in our tables.

We should note that there was not only a quantitative, but also a qualita-
tive difference in the conditioned response to light under the influence of the
magnet and when just the solenoid switching procedure was performed. Since the
lag period of the conditioned response to light averaged 2 sec, we considered
a lag period of more than 3 sec to represent inhibition, and a lag period of
less than 1 sec to represent acceleration. Table 20 shows the qualitative dif-
ference between the real and the false applications of the magnet.

TABLE 20. CHARACTER OF THE LAG PERIOD OF THE CONDITIONED
RESPONSE TO LIGHT UNDER THE INFLUENCE OF THE MAGNETIC FIELD
OR THE SOLENOID SWITCHING PROCEDURE.

Character of the
influence

Number
of appli-
cations

Number of cases

acceleration

normal

inhibition

raw

— : — : — r.' . — :

magnet

false solenoid
switching

50
50

26
38

52
76

24
4

48
8

When the solenoid switching procedure was carried out, the lag period of
the conditioned reflexes did not change in most cases (76%), decreased in 16%
of the cases, and increased only in 8% of the cases. Under the influence of the
CMF in approximately half of the cases we observed an increase in the lag per-
iod, but, characteristically, we did not encounter even one case of a decrease
in the lag period.

144

Under the influence of a CMF the response to light, if it existed, was ex- /172
pressed in a single pecking motion. The response usually occurred within the
first 3 sec, and in the remaining time of the application of light with the
magnet, i.e., 17 sec, the pigeon did not peck the bar. When only the solenoid
switching procedure was performed, the pigeon pecked the bar throughout the time
of the light effect. Thus, the inhibiting effect of a magnetic field on pigeons
appeared once more.

The widespread opinion that both a positive and a negative conditioned re-
flex can be developed for any stimulus that the animal perceives did not agree
with our results. Therefore, we decided to check whether development, i.e.,
the formation of the inhibiting process during the time when we did not rein-
force the CMF + light stimulus with food, occurred at all. The basic index for
the magnitude of the positive reflex in our tests is the lag period of the con-
ditioned reflex to the conditioned stimulus. Changes of this index during the
tests on development of conditioned inhibition to a magnet must characterize
the process of forming a time relationship. To calculate all the cases of the
response to the CMF + light stimulus we designated the absence of the conditioned
response by a lag period equal to 25 sec, although the conditioned stimulus
acted for only 20 sec. This allowance was made for convenience of numerical
treatment of the material and it could help explain why there is only a tenden-
cy towards a definite change in the magnitude of the response lag period under
the influence of the CMF + light stimulus. The results of each test were treat-
ed in the following manner. We determined the average lag period of the condi-
tioned reaction to light before the application of the CMF in the test, i.e.,
we calculated the lag period of the reactions in which sequential inhibition
from the influence of the CMF could not result. Furthermore, we calculated the
average (for the test) lag period of the response to the CMF + light stimulus.
Thus, on the graph we obtained 2 curves that characterized the dynamics of the
lag periods of the response to light under the influence of the magnet and in
its absence. But the lag periods of the conditioned responses change over a
significant range from test to test, and determining the direction of the
change required additional treatment of the results. We applied the graphic
distribution method of treating the results: the method of the moving average.
The majority of separate indices participate several times in the formation of
the average index. Thus, we found the average value of 6 indices. We obtained
the first point on the graph by determining the average magnitude of the lag
period from the 1st to the 6th test, the 2nd point from the 2nd to the 7th test,
the 3rd point from the 3rd to the 8th test, etc. The curves of the response
lag periods thus developed for 3 pigeons are shown on the graphs of Figure 59.
These graphs clearly show that in all pigeons the lag period of the response /174
to the CMF + light stimulus exceeds the lag period of the response to light.
I.e., the inhibiting effect of the magnet is observed throughout the tests.
The degree of the inhibiting effect depends upon the characteristics of the in-
dividual pigeons, but the intensity of this inhibition did not increase as the
number of combinations increased. If we compare the average graph for the 3
pigeons, we will see that the curve of the lag period of the response to CMF +
light has a tendency towards rectilinearity, i.e., conditioned inhibition does
not develop in pigeons.

But if conditioned inhibition is not formed, then the CMF acts according

145

IS
13
t1
3
7
5
3
1

\ f7\

. tS
^ /J

9
7
5
3
I

If
9
7
5
3
1

■ I I I I ■ I 1 I I 1 1 I I t I I I I I I I I I

/ 3 5 7 3 f1 13 15 17 19 21 23 25 27 29 31

I ■ I ■ ■ ■ ■ ■ I ■■ I I I 1 I I ■ 1 1 1

/ 3 5 7 3 If 13 15 17 19 21 23 25 27

■ ■ ■ f ■ ■ I ■ ■ ■ I I I I I

1 3 5 7 9 11 13 15 17 13 21 23 25
(],) tloMep onoima

Figure 59. Dynamics of the Averaged Lag Per-
iod of the Conditioned Responses to a Constant
Magnetic Field + Light (1) and to Light (2) in
Pigeon No. 1 (A), No. 2 (B) and No. 3 (C) .
Key: (a) Lag Time, Sec; (b) Number of the
Test.

+ light stimulus was sometimes equal to the lag
sometimes it exceeded it, but most often it was

to the principle of exter-
nal inhibition. Then, an
increase in the lag period
of the conditioned response
must also occur during food
reinforcement of the effect
of the CMF + light stimulus.
We observed this in pigeon
no. 2. As the number of
combinations of the CMF +
light stimulus with food in-
creased, the lag period of
the response did not de-
crease, but even increased
somewhat, although the lag
period of the response to
light remained at the same
level .

To check whether the
conditioned response to
light is inhibited under the
influence of any additional
stimulus, we applied light
together with a gurgling
sound, beginning this sound
stimulus, like the CMF, 10
sec before the light was
turned on and stopping it
together with the light.
Figure 60 shows the magni-
tudes of the lag period of
the response to light, CMF
+ light and gurgling +
light observed during one
test. Alternating these
stimuli allows us to com-
bine them in 5 groups, each
of which includes the mag-
nitude of the lag period
of the response to all 3
different stimuli. Among
the groups, the lag period
of the response to the CMF /175
+ light stimulus always ex-
ceeded the lag periods of
the responses to the other
stimuli. The lag period of
the response to the gurgling
period of the response to light,
less than it. In selecting the

146

average (for the test) magnitudes of the lag period, we should note that the
lag period of the response to CMF + light exceeded the analogous indices of the
response to light and to gurgling + light by 2 times. The lag periods of the
response to light and to gurgling + light differed insignificantly, so that they
can be considered equal.

Consequently, a CMF also has
an inhibiting effect when it is
reinforced with food, while in
combination with other stimuli
(gurgling) the response to light
is not inhibited, although the
number of applications of the com-
bination of gurgling + light stim-
uli was great, and we could expect
an inhibiting effect under the in-
fluence of this combination due
to the orienting effects. Perhaps
the gurgling did not cause exter-
Figure 60. Lag Periods of the Conditioned ^^^ Inhibition because the condi-
Response to Light (1) , a Constant Magnetic tioned reflex to light was extreme-
Field + Light (2), and Gurgling + Light (3), ^^ stable, since several hundred
for One Test. A-E = Different Measure- combinations of light with food
ments During the Test; F = Averaged In- reinforcement were given.

dices. Key: (a) Lag Time, Sec. .n ^u ...

All the material given above

on the development of conditioned
reflexes to a magnetic field in pigeons allows us to conclude that in our tests
we observed only the unconditioned effect of a magnetic field on birds, which
was expressed in inhibition of the conditioned reflex to light and in an in-
crease in the number of intersignal reactions.

The Development of Conditioned Reflexes to
Electromagnetic Fields in Fish

The basic subjects for investigation in this series of tests were carp,
goldfish and stickleback. Positive conditioned reflexes were developed to a
CMF, a variable magnetic field with a frequency of 50 Hz, a UHF field and ioniz-
ing radiation. The electrodefensive method was basically used.

In this series of tests we developed both positive and inhibiting condi-
tioned reflexes to a magnetic field using both the food-getting and defensive
methods .

Development of a Positive Conditioned Reflex

The food-getting method . Table 21 gives the results of developing food-
getting conditioned reflexes to a CMF in five goldfish. As the CMF source we
used a cobalt magnet and a battery-powered electromagnet. A CMF was created
in the aquarium with a field strength gradient from 2 to 200 Oe.

/176

147

TABLE 21. CHARACTERISTICS OF THE POSITIVE CONDI-
TIONED REFLEX TO A MAGNETIC FIELD IN GOLDFISH.

Number of

combinations

Within

the first

100 combinations

before the

lag

number of

appearance

fixation of

•r4

period.

intersignal

of the re-
flex

the reflex

sec

reactions

11.5

234

12.5

114

13.3

8.7

15.0

aver-

ages

12.2

100

Judging by the rate of fixation of the conditioned reflex, the degree of
stability and the magnitude of the lag period, the response to the magnetic
field is similar to the response to light. But we observe a sharp difference
between these reflexes in the number of intersignal reactions. While during
the development of the conditioned reflex to light there was an average of 38
intersignal reactions to 100 combinations, during the development of the reflex
to the magnet the average number of intersignal reactions for the same number
of combinations was 100. This difference in the number of intersignal reactions
is observed not only for the first 100 applications of the conditioned stimulus,
but it is retained over several hundred applications.

The possibility of developing a food-getting conditioned reflex to a CMF
in fish (Figure 61) is illustrated by the kymograms of the tests.

The electrodefensive method . In most tests by this method we used a vari-
able magnetic field with a strength of 100 Oe created by a solenoid that was
fed from a city power line. The conditioned electrodefensive reflexes develop-
ed to a variable magnetic field appeared at the same rate as the corresponding
reflexes to a CMF and differed only in greater stability. Therefore, we assum-
ed that a constant magnetic field and a 50 Hz variable field have a similar
mechanism of effect on fish and in future tests we did not compare the effect
of these physically different agents. All tests were conducted on carp 6-10 cm
long.

The positive conditioned reflex was developed in all fish. The possibili-/177.
ty of developing a positive conditioned reflex is illustrated by the kymograms
of the tests (Figure 61, B) . The results of the tests are combined in Table 22.
If we compare them with the results of developing a positive conditioned reflex
to a magnetic field in goldfish through the food-getting method (Table 21) , we

148

Ma Ma Ma Ma Ma Ma Ma Ma

"Vr^ T y L[ IT U T

3 Z 33 jy 3f 36 37 38 39

^ I [ 1

f BmmmammMmMmBmMBmBmMmmmMmmmmmMmmmmmmmmmm.

3V ii / id 2 57 3 58

Figure 61. Kymograms of Tests Concerning Development of Time Re-
lationships to a Magnetic Field in Fish. A = Development of the
Positive Conditioned Reflex by the Food-Getting Method; B = De-
velopment of the Positive Conditioned Reflex by the Defensive Me-
thod; C = Development of Conditioned Inhibition by the Food-Getting
Method; 1 = Marking of the Conditioned Reaction; 2 = Marking of the
Influence of the Conditioned Stimulus; 3 = Marking of Reinforcement;
4 = Time Marking with a 5-Second Interval. The Remaining Designa-
tions are the same as in Figure 58. Key: (a) MF = Magnetic Field;
(b) L = Light; (c) L + MF = Light + Magnetic Field.

note that with the food-getting method the reflex is formed more quickly and is
more stable. With the food-getting method the reflex to a magnetic field, on
the average, appears after 5 combinations, becomes fixed after 26 combinations
and attains a stability of 61%, but with the electrodefensive method the reflex
appears, on the average, after 11 combinations, becomes fixed after 57 combina-
tions and attains a stability of 39%.

To compare the effect of a magnetic field with the effect of other stimuli
on fish, we give the results of another series of tests on the development of
positive conditioned reflexes to light and to a bell in fish (Table 23) .

The introduced data allow us to conclude that conditioned reflexes to lYlZ
light and to a bell are formed in fish, on the average, at an identical rate:
they appear after 8-9 combinations and are fixed after 15-20 combinations;
but the reflex to light is more stable (56%) than the conditioned reflex to the
bell (42%).

Comparing the results of Tables 22 and 23, we see that the conditioned
reflex to a magnetic field yields in both rate of development and degree of

149

TABLE 22. CHARACTERISTICS OF THE POSITIVE CONDITIONED REFLEX TO A
MAGNETIC FIELD IN FISH, DEVELOPED BY THE ELECTRODEFKNSIVE METHOD.

Number of

combinations

&«

combinations

•

•u

•H

before

appear-

fixa-

•rl

appear-

fixa-

1 k

ance of

tion of

■^

ance of

tion of

the re-

(Zl

flex

102

aver-

ages

TABLE 23. CHARACTERISTICS OF POSITIVE CONDITIONED REFLEXES TO LIGHT /179
AND TO A BELL IN FISH WHEN THE ELECTRODEFENSIVE METHOD IS USED.

•

Bell

Light

Number of

■

combinations

!>.

4-1
•H
.-1
•rl

-S
4J

•H

■
before
appear-
ance of

before
fixa-
tion of

■H
H
•H
Xi

before
appear-
ance of

before
fixa-
tion of

the re-

4J
C/3

the re-

flex

—

4
5

2
6

56
49

150

TABLE 23. (Continued)

Bell

Light

Number of

•H
P4

combinations

•H
<-i
•H

combinations

■U
•H

•H

before
appear-

before
fixa-

before
appear-

before
fixa-

ance of

tion of

ance of

tion of

43
CO

the

the re-

flex

■

average

s 8

Note: the dash indicates that the reflex was not developed, the
? indicates that it was not fixed.

stability to the light and sound reflexes, which characterizes the magnetic
field as a weak stimulus.

Development of Conditioned Inhibition to
the CMF + Light Stimulus

Development of conditioned inhibition to a magnetic field was conducted on
5 goldfish by the food-getting method and on 7 fish (3 goldfish and 4 stickle-
back) by the defensive method. The overall results are given in Table 24.

The data of Table 24 show that with the use of different methods the rates
of formation of the conditioned reflex in goldfish are approximately identical.
With the food-getting method, on the average, the reflex appears after 3 com-
binations and is fixed after 20 combinations of light with food, and with the
defensive method it appears after 5 and is fixed after 23 combinations of light
with the mechanical stimulus. In stickleback the conditioned reflex is fixed
somewhat later (on the average, after 32 combinations) than in goldfish (on the
average, after 23 combinations). The stability of the conditioned reflex to
light is approximately identical and it averaged 60% in both species of fish
when different methods were used.

The lag period of the reaction to light is somewhat longer in stickleback /179

151

TABLE 24. CHARACTERISTICS OF THE CONDITIONED REFLEX TO LIGHT, THE
DEVELOPMENT OF WHICH PRECEDRD THE FORKA.TION OF CONDITIONED II41IIBITI0N.

7180

to ji

<u m

H -H

O >M

ft>«

•H

Number of combinations

before
appear-
ance of
the re-
flex

•O

r-j

Jii

a ^

•H

■u rt

w ^

before
fixa-
tion of
the re-
flex

•H
•5

lag
period,

sec

Food-Getting

9
13

7
15
44

Defensive

31
10
29

25
11
72

11.9

15.0

15.8

16.2

17.0

e
58

3.1

4.9

5.9

7.9

6.3

5.5

5.3

No. of inter-
signal reac-
tions for 100
applications
of the condi-
tioned stimulus

56
20
68
18
28

TABLE 25. RATE OF FORMATION OF CONDITIONED
INHIBITION TO THE CMF + LIGHT STIMULUS IN FISH.

Number of

stimu-

M J3
(U 10
•H -H

0)
Pl< M-l

•

lus appli

cations

before ap-

before fix-

pearance of

ation of

inhibition

Food-Getting

•H
14-4
T)

5
6
7

1
2
1

3
14
18

152

TABLE 25. (Continued)

Number of

stimu-

to ^
(U to
•H -H

lus applications

before ap-

before fix-

CO o

pearance of

ation of

Inhibition

inhibition

Defensive

13 J3

rH to
O 4-1

9
10
11

1
3
1

8
15
11

1
2

?
5

•H O
4-1 0]

1
1

(on the average, 6.1 sec) than in goldfish (on the average, 4.6 sec). The
average number of intersignal reactions in the first 100 combinations is 38.

We began to apply the magnetic field after fixation of the conditioned re-
flex to light. Table 25 gives the rate of the appearance and fixation of con-
ditioned inhibition to CMF + light.

In most of the fish studied, inhibition appeared during the application of
the CMF + light stimulus which indicates the effect of the CMF as external in-
hibition, and was fixed within 15 applications of this combination of stimuli.
Inhibition appeared and was fixed somewhat more rapidly in stickleback and in
goldfish.

The possibility of the appearance of inhibition to a CMF is illustrated /181
by the kymograms of the tests taken during use of the food-getting method (Fig-
ure 61, C) , and the records of tests conducted by the defensive method.

In fish no. 7 and 8 we sometimes observed a general negative reaction
during the influence of the CMF. Usually the fish swam in no particular direc-
tions during the influence of the stimuli and in the intervals between them,
but during the influence of the magnet they sometimes swam rapidly to the oppo-
site corner of the aquarium as if they were trying to get as far as possible
away from the magnet. After removal of the magnet the fish never repeated
these movements.

The material introduced shows that conditioned inhibition to a CMF is easi-
ly formed in fish. As is evident from the test recordings, the inhibiting ef-
fect of a magnetic field is frequently not limited to the time of application
of the magnet, but also appears in sequential inhibition. This sequential

153

TEST NO. 13, GOLDFISH NO. 9, 4 MAY 1954

Stimulus
number

Time of the start of
the stimulus influence

Stimulus

Lag peri-
od, sec

Notes

161
162
163
164
27

11 hr 04 min 00 sec
11 hr 4 min 30 sec
11 hr 5 min 10 sec
11 hr 5 min 50 sec
11 hr 6 min 30 sec

light
II

CMF + light

165
28

11 hr 7 min 10 sec
11 hr 7 min 40 sec

light
CMF + light

166

167

11 hr 08 min 15 sec

11 hr 9 min

11 hr 9 min 30 sec

light
II

CMF + light

sequential
inhibition

168
30

11 hr 10 min 00 sec
11 hr 10 min 50 sec

light
CMF + light

169

11 hr 11 min 30 sec

light

sequential
inhibition

TEST NO. 16, GOLDFISH NO. 10, 10 SEPTEMBER 1954

Stimulus

Time

of the start of

Stimulus

Lag peri-

Notes

number

the s

timulus influence

od, sec

280

9 hr

13 min 00 sec

light

281

9 hr

15 min 00 sec

282

9 hr

17 min 00 sec

283

9 hr

19 min 00 sec

284

9 hr

21 min 00 sec

285

9 hr

23 min 00 sec

9 hr

25 min 00 sec

CMF + light

286

9 hr

27 min 00 sec

light

9 hr

29 min 00 sec

CMF + light

—

287

9 hr

30 min 30 sec

light

9 hr

33 min 00 sec

CMF + light

—

288

9 hr

34 min 30 sec

light

9 hr

36 min 00 sec

CMF + light

289

9 hr

39 min 00 sec

light

sequential

9 hr

41 min 00 sec

CMF + light

inhibition

290

9 hr

42 min 30 sec

light

154

TEST NO. 14, GOLDFISH NO. 11, 8 SEPTEMBER 1954

Stimulus
number

Time of the start of
the stimulus influence

Stimulus

Lag peri-
od, sec

Notes

251
252
253
254
255
11

17 hr 28 min 00 sec
11 hr 29 min 00 sec
11 hr 31 min 00 sec
11 hr 32 min 30 sec
11 hr 34 min 00 sec
11 hr 35 min 30 sec

light

It
II

CMF + light

3
7
3
7
5
10

256
12

11 hr 37 min 00 sec
11 hr 38 min 30 sec

light
CMF + light

257
13

11 hr 40 min 00 sec
11 hr 41 min 30 sec

light
CMF + light

sequential
inhibition

258
14

11 hr 43 min 00 sec
11 hr 44 min 30 sec

light
CMF + light

sequential
inhibition

259
15

11 hr 48 min 00 sec
11 hr 49 min 30 sec

light
CMF + light

sequential
inhibition

260

11 hr 51 min 00 sec

light

TEST NO. 6, STICKLEBACK NO. 1, 21 JUNE 1956

Stimulus
number

Time of the start of
the stimulus influence

Stimulus

Lag peri-
od, sec

Notes

71
72
73
74
2

15 hr 00 min 00 sec
15 hr 00 min 50 sec
15 hr 1 min 20 sec
15 hr 2 min 00 sec
15 hr 2 min 40 sec

light
II

CMF + light

12
4
2
2

11 hr 3 min 25 sec

11 hr 4 min 20 sec
11 hr 5 min 00 sec
11 hr 5 min 40 sec
11 hr 6 min 25 sec

light
II
II
II

CMF + light

4
7

sequential
inhibition

79
80

11 hr 7 min 30 sec
11 hr 8 min 20 sec

light
II

sequential
inhibition

155

TEST NO. 11, STICKLEBACK NO. 4, 23 JUNE 1956

Stimulus
number

Time
the 8

of the start of
timulus influence

Stimulus

Lag peri-
od, sec

Notes

101
102
103
104
6

9 hr
9 hr
9 hr
9 hr
9 hr

00 min
1 min
1 min

3 min

4 min

00 sec
00 sec
20 sec
00 sec
15 sec

light
11

CMF + light

2
2
4
3

105
106

9 hr
9 hr
9 hr

6 min

7 min

8 min

00 sec
30 sec
45 sec

light
II

CMF + light

sequential
inhibition

107
108
109
110

9 hr
9 hr
9 hr
9 hr

10 min

11 min

12 min

13 min

00 sec
15 sec
20 sec
25 sec

light

M
II
II

sequential
inhibition

inhibition observed in tests with the use of the defensive method cannot be ex-/182
plained by the fact that the conditioned-inhibiting combinations of stimuli are
not reinforced and thereby suppress the conditioned reflex to light. As was
stated in the description of the defensive method, the conditioned stimulus was
not reinforced if the conditioned response was observed during its influence.
Consequently, the fact of sequential inhibition can only be explained by the
influence of the CMF itself.

To quantitatively characterize the observed properties of the magnetic
field, in Table 26 we have generalized all the cases of inhibition encountered
in developing conditioned inhibition to a CMF in fish.

The tabular data show that sequential inhibition is encountered in stickle-
back more often (on the average 73% of the cases) than in goldfish (on the /183
average, 36%). In the latter, sequential inhibition is encountered more fre-
quently with the use of the food-getting method (on the average, in 47% of the
cases) than with the use of the defensive method (on the average, in 36% of the
cases) . We obtain similar comparative data in an analysis of the intensity of
sequential inhibition. Sequential inhibition is expressed in complete inhibi-
tion of one or several reactions to light more frequently in stickleback than
in goldfish. An intensive sequential inhibition is observed in goldfish signi-
ficantly more often with the food-getting method than with the defensive method.

The data of Table 26 indicates that cases of sequential inhibition alone ^ /184
are encountered approximately 10 times less often than cases of immediate inhi-
bition of the conditioned light reaction. But cases of sequential inhibition
alone are encountered more frequently in goldfish with the defensive method,
i.e., in Just those cases when the general inhibiting effect of the magnetic

156

•

O En
M

M
H
M

o m

C/1

[t1

PLJ

1-1

■<J

•

l-l

Crt

>*-i to 1

O <U Id bO

. 2 '"^ f^

B^a

^O rH

<u o ri -u o

<»

CT\

t»

r^ 00

< 1 1 1

g r^ ^14-1

00 00

CTi

^ CM rH I^

9 H M-i -H m

I-l

VO -*

■<t

rH CM

rH 60

iw n) (d

o m -H -rt

S^S

r^ 00

<t-

O 4-1 -U

1 1 1 1

!^ Id -H

(U CO a) ^ (U

•9 (U 3 -H C

a eg D-^ o

3 n) a) Id .H
Z o to -H n)

vo m

rH
rH

O 1 rH O

O r^

6-S

<J-

CM ~a-

-<}•

1 1 1 1

r~.

<»

vO 00

~* rH CM VO

to
u

CM CM

rH rH

(U o

n s

O 4-1

■H to

3
U CO

r-l

o en

O O CM CM

■sT

4-1 3

O ,-1

o jd

4-1 O

cC 3

"4-4 4J

O -H

■u

<u a

U t{

CO -H

Q) XI

0) to

to -H

o Id

4-1 4J

i~-

O rH

CM O CM CO

•r4

H-l SO

U-l

O -H

O H

.-1
(U

Vj -H

O 0)

<U 4-1

Id jd

-9 Id

(U u

0) 3

§ 2!

S -H

5 E

.a o

° 2

ty>

m i~>

CM O CO rH

vi3

2; D*

ca 4-1

O 4J

m to

H rH

poxaad S^ej

UT asBaaouT

o rH m o

, td

u-i a) o

6-S

O CO

o y-i 4-1 -H

1^ r^

1-^

1 1 1 1

O (0 4-1

0) to "^3 ^

^ (U <U -H

T-i

CM CO

■* CM o r~.

§ 2 i -S

<-i

vo <t

^ CM

3 cfl i s

Z O -H •!-(

s3j:nsodx3 jo -on

0\ ON

<-{

O- eg CM r-

t» in

^ eg

-3-

aequinu qsfiL

vi3

1^ 00

rH
rH

rH CM CO <!■

•H

U CO

1 -H

O -Q

T3 4J

M-l (U

4-1

14-1

O 0)
h 60

rH M

CO o

4-1 -H

qsTj JO SBToads

qsTjpxog

>I0Fq3X5[0T5S

O 4-1
H CO

157

field is expressed more weakly. As in the analysis of the number of cases of
sequential inhibition, in an analysis of the general inhibiting effect of a
GMF V7e are surprised to find that the effect of the magnet is observed in
stickleback more frequently (on the average, 98%) than in goldfish (on the aver-
age, 78%), and in goldfish the inhibiting effect of the magnet is observed more
frequently with the food-getting method (on the average, 89%). It is evident
that in stickleback the inhibiting effect appears almost with each application
of the CMF, and that this effect is most frequently expressed in immediate in-
hibition of the conditioned light response. Cases of the magnet delaying both
the immediate and a subsequent light response are encountered rather frequently,
and once we noted the effect of the CMF appearing only in sequential inhibition.

Sometimes the effect of the magnetic field was not limited to immediate
and sequential inhibition. After the initial applications of the CMF we ob- /185
served a stable disturbance of the conditioned response to light, lasting
several days and appearing even in cases when the CMF was not applied. In the
analysis of this disturbance of the stability of the light reflex, our material
is distributed just as in the analysis of the other indices of the inhibiting
effect of the CMF, i.e., the stability of the light reflex is disturbed most
strongly in stickleback; to a lesser degree in tests on goldfish conducted by
the food-getting method; and the stability of reflexes in goldfish almost does
not change, or is even increased, with the use of the defensive method.

As the tests with pigeons showed, the inhibiting effect of a CMF cannot be
accompanied by the development of a time relationship. Therefore, as we did
during treatment of the results of tests on pigeons, we made special calcula-
tions of the lag period of the response to CMF + light during development of
conditioned inhibition in fish no. 4, 5, 6, 7 and 8. Figure 62 shows that,
although the inhibiting effect of the magnetic field appears during the first
applications of the complex CMF + light stimulus, this effect becomes more in-
tensive as the number of applications of the complex stimulus without food is
increased. Consequently, the external inhibition arising in the first applica-
tions of the combination of CMF + light stimuli passes into internal inhibi-
tion as a result of not reinforcing this combination. Thus, the development of/187
both positive and negative conditioned reflexes to a magnetic field occurs in
fish. But the magnetic field retains the properties that appear in the tests
on pigeons: i.e., development of a positive conditioned reflex to the magnet
is characterized by an excess of interslgnal reactions, and the magnetic field
exerts primarily an inhibiting effect on predeveloped conditioned reflexes.

We should also note that several tests conducted together with G. L.
Verevkina concerning the effect of a magnetic field on conditioned reflexes.
These investigations were conducted on 4 fish: 2 bullheads and 2 flounders.
Using the defensive method, in these fish we developed positive conditioned re-
flexes to light and sound. We alternated applications of sound and light
throughout the test. If there was a response to the conditioned stimulus, we
did not give reinforcement. After the reflexes were developed, in separate
tests with the aid of a solenoid we created a 100-200-Oe CMF. The solenoid
was switched on 20 sec before the conditioned stimulus and was switched off
together with it.

The effect of the CMF on conditioned reflexes is illustrated in Figure 63.

158

:'5

ortS

¥0

^^^^^Ciz.

i/^-^o**

•"x^ /^*~*

A.^4

\>/

3¥5B183W

f 5 6 7 S 9 W 11 tZ 13
(b) /fO-MCfj ufioifna

Figure 62. Dynamics of the Lag
Period of Conditioned Reflexes
to CMF + Light in Goldfish Dur-
ing Development of Conditioned
Inhibition to this Stimulus.
The Number of the Fish is Shown
on the Curves. Key: (a) Time
of Lag, Sec; (b) Number of the
Test.

cases for both the light and the
in stability is encountered more
with the light reflex (7%) .

In each test in which the magnetic field
was applied the stability of the light re-
flex was reduced in bullhead no. 1. The
stability of the sound reflex was reduced in
two cases of the application of the magnetic
field, but in one test we observed an in-
crease in the stability of the auditory re-
flex during the influence of the CMF.

With flounder no. 3 the stability of
the light conditioned reflex was reduced
during each application of the magnet, and
the reflex to the bell was inhibited in 3
out of 5 cases. Sometimes the magnet destroy-
ed all conditioned-reflex activity (tests
no. 25 and 32), although each conditioned
stimulus was accompanied by unconditioned
reinforcement .

The general results show that a CMF /188
most frequently inhibits conditioned reflex-
es, and that the reduction in stability of
the light conditioned reflex appears more
sharply than that of the sound reflex. Thus,
the stability of the light reflex is reduced
in 85% of the cases, and that of the sound
reflex in 70%. Absence of any effect from
the magnetic field is observed in 7% of the
sound conditioned reflexes, and an increase
frequently with the sound reflex (22%) than

Thus, a CMF applied 1-4 times during the tests and throughout the tests
has primarily an inhibiting effect on predeveloped time relationships.

We conducted a series of tests to determine the threshold of perception of
a magnetic field in fish during development of a positive conditioned reflex to
this stimulus. The tests were conducted on 3 fish by the food-getting method /189
(goldfish no. 1, 2 and 3) and on 3 fish by the electrodefensive method (carp
no. 11, 13 and 14). We varied the magnetic field strength with a rheostat.
Similar results were obtained on all 6 fish. From the test records which follow
it is evident that the conditioned reflex to a magentic field disappears at a
current of 0.25-0.37 amp which corresponds to a magnetic field strength of 10-30
Oe.

The threshold strength of the magnetic field (10-30 Oe) was approximately
10 times lower than the highest strength which we used in our tests to obtain
a conditioned positive reflex and conditioned inhibition in fish.

In the tests with goldfish no. 2, in which we developed the food-getting
positive conditioned reflex to a magnetic field with a strength of approximately

159

100 Oe, in 8 tests (110

X W

(a) BO

28 ® -W ® 32 33 3V ® 36 @ 38

^ gfi ■/.■/ 28 @ 3(1 .V @ 33

Figure 63. The Effect of a Magnetic Field on
the Stability of Light (1) and Sound (2) Condi-
tioned Reflexes in Fish. A = Bullhead No. 1;
B = Flounder No. 3. The Number of the Tests in
Which a Magnetic Field was Applied are Circled.
Key: (a) Stability of the Reaction, %; (b) Num-
ber of the Test.

annix cations)

»e later used

a constant magnetic field
on the order of 10,000 Oe
created by a special elec-
tromagnet at the physics
department of Moscow State
University.

The basic indices of /190
the conditioned reflexes
to the 100 and 10,000-Oe
magnetic fields are com-
pared on Figure 64. It is
evident that the stability
of the conditioned reflex
did not change with the
sharp increase in the in-
tensity of our stimulus,
but the average lag period
was reduced from 14.8 to
10.5 seconds and, what is
particularly interesting,
the number of intersignal
reactions increased by ap-
proximately one and a half
times. Of course, the in-
crease in the number of in-
tersignal reactions can be
explained by a variety of
reasons, but we tried not
to change the test condi-
tions, except for increasing

>4-(

Application of

Time of turning

„

u to

•H C

•rl

the stimulus

on the solenoid

•a o

4-1

o m

u <u

•H

Food-Getting Method

goldfish no. 2,

543

12 hr 56 min 15 sec

0.37

test no. 51,

544

12 hr 58 min 10 sec

0.37

18 December 1954

545

13 hr 01 min

0.25

546

13 hr 03 min 25 sec

0.37

547

13 hr 05 min 30 sec

0.37

548

13 hr 07 min 50 sec

0.37

160

TABLE (Continued)

Application o
the stimulus

Time of turning
on the solenoid

C!
O

O 4J
•H
(U TJ
O ti
O

•H
(0

u
u

0) o
w

0) 0)

eu u

a)
u

Food-Getting Method

goldfish no. 2,

549

13 hr 11 min

0.25

test no. 51,

550

13 hr 14 min 05 sec

0.37

18 December 1954

551

13 hr 16 min 30 sec
Defensive Method

0.37

carp no. 11,

303

18 hr 21 min 30 sec

0.50

test no. 27,

304

18 hr 23 min 15 sec

0.50

30 December 1954

305

18 hr 25 min 00 sec

0.50

306

18 hr 26 min 45 sec

0.25

307

18 hr 29 min 15 sec

0.37

308

18 hr 31 min 00 sec

0.37

309

18 hr 33 min 15 sec

0.25

310

18 hr 35 min 16 sec

0.25

311

18 hr 38 min 00 sec

1.00

"f,8

f0,5

i
lI

D/ m

the intensity of the stimulus; therefore, we relate the
increase in the number of intersignal reactions to the
increase in the magnetic field intensity, although ad-
ditional experimental material is needed for a final
judgement.

Thus, the stability of conditioned reflexes to a
magnetic field did not change when the intensity of the
stimulus was increased by two orders of magnitude. We
did note a certain increase in the number of intersignal
reactions. In other words, a quantitative increase in
the intensity of the magnetic field did not bring it
close in physiological effectiveness to such stimuli
as light or sound.

Figure 64. Stability in % (A); Lag Period in Sec (B)
and Number of Intersignal Reactions (C) During Develop-
ment of the Food-Getting Conditioned Reflex to a 100-Oe
(1) and 10,000-Oe (2) Magnetic Field.

161

Development of Conditioned Reflexes to a
UHF Field in Fish

The experiments were conducted on 6 carp, of which 2 were preliminarily
blinded (no. 3 and 5) and the forebrain was removed from one (no. 6). The gen-
eral results of the tests are given in Table 27. It is evident that, on the
average, the reflex appears after 12 combinations, does not become fixed m all
fish and attains a stability of 45%.

TABLE 27. CHARACTERISTICS OF THE ELECTRODEFENSIVE
CONDITIONED REFLEX TO A 500 v/m UHF FIELD IN FISH.

•

Number of combinations

Stabil-
ity, %

Average
lag pe-
riod, sec

before the
appearance
of the re-
flex

before the
fixation of
the reflex

1
2
3
4

5
6

42
44
53
42
47
45

3.0
3.0
3.6
3.5
3.5
3.2

aver
age

3.3

Development of Conditioned Reflexes to
Ionizing Radiation in Fish

Contemporary physiology affirms that many types of energy (mechanical,
thermal, electrical, etc.) can affect an organism as stimuli under definite
conditions. However, the question of whether ionizing radiation is a stimulus /19i
or not is still debated. In the radiobiological literature there are works
(although sometimes contradictory) showing the ability of ionizing radiation
to cause behavioral reactions in animals [Tsypin, 1964].

The phenomenon of so-called radiophosphene was established early in the
development of radiobiology [London, 1904; Lipetz, 1955; Pape and Zakovsky,
1954; and others]. This phenomenon is defined as the sensation of a weak glow
during the impingement of a beam of ionizing radiation on the dark-adapted
human eye. Another group of works [Hug, 1958; Born, 1960; Andrews and Cameron.
1960; Overall et al. , 1959; Khrushchev, Darenskaya and Pravdina, 1961; and
others] introduced facts concerning the defensive reaction of animals to radia-
tion. In this case, the reaction is manifested as the animal's avoiding the

162

radiation beam or in characteristic protective maneuvers. Finally, a third
group of works indicates that it maybe possible to develop conditioned reflexes
in animals through the use of ionizing radiation as an unconditioned stimulus
[Movsesyan et al. , 1954; Livshits, 1961; Garcia et al. , 1957; and others].

The problem of this investigation was to check the possibility of using
ionizing radiation as a conditioned stimulus. This work was conducted together
with A. B. Tsypin.

The tests were conducted on 11 yearling carp. The use of fish as the sub-
jects for our studies is explained by their facile development of conditioned
reflexes to different stimuli, and also by the convenience of recording condi-
tioned responses in fish, which could move freely about the aquarium.

Irradiation of the fish before development of conditioned reflexes did no t/ 192
cause motor reactions (Figure 65, A). After several combinations of irradia-
tion with an electric stimulus the fish began to move during irradiation alone
(Figure 65, B) . The appearance of a conditioned reflex was observed following
4-13 combinations (Table 28); however, we did not manage to obtain a stable
conditioned reflex in any fish, although some of them were given up to 250 com-
binations. In 4 fish, we could observe fixation of the conditioned reflex, but
in the remaining fish it was not fixed.

TABLE 28. CHARACTERISTICS OF CONDITIONED RE-
FLEXES TO IRRADIATION IN NORMAL AND OPERATED FISH

Number of combinations

rH B-S

•H

before the

appearance

fixation of

•r4

of the re-
flex

the reflex

CO Ti

Normal fish

Blinded fish

Fish with hemispheres remove

163

The stability of the conditioned reflexes varied from 31 to 51%. This
stability was already observed from the 3rd through ttie 5th test. Tn subsenuent
tests, in spite of the increase in the number of combinations, the number of ^
conditioned responses did not increase. Sometimes we even noted a decrease in
the stability of the conditioned reflex as the number of irradiations increased.
It is possible that in this case the overall effect of irradiation leads to
suppression of the conditioned reflex to irradiation itself.

These results definitely demonstrate the possibility of developing con-
ditioned reflexes to irradiation in fish. While confirming the effect of ir-
radiation on the behavior of fish, our data also indicate the peculiarities of
this effect. Although it appears strongly, the conditioned reflex is poorly
fixed and it does not attain the stability that is observed in the development
of conditioned reflexes to ordinary stimuli.

Since there is proof that it is /194
possible to perceive Y irradiation
through the visual analysor, our first
assumption about the mechanism of the
effect of irradiation on a fish was a
hypothesis concerning the effect of ir-
if radiation through the retina. However,

, m ...... n ■•■■■■■ 1 1 1 1 1 M 1 ' tests with 2 blinded fish that had the

entire eyeball removed on both sides

/ showed that conditioned reflexes to Y

' ' *" ~ irradiation also developed in these

_Z. fish. The conditioned reflexes of blind-

■ ~ ed fish did not differ at all from those

t_^ 3 of intact fish (Table 28).

If The next stage of our work was de-

II 1 1 ■ 1 1 11 ■ I ■ 1 1 III velopment of conditioned reflexes to

irradiation in fish after removal of
> the forebrain. This series of experi-

Figure 65. Kymograms of Tests on De-
veloping Electrodefensive Conditioned
Reflexes to Ionizing Radiation in Fish.
A = Absence of the Defensive Reaction
y to Irradiation before Development of

I u 1 1 1 1 1 II 1 1 1 1 1 1 1 1 1 M 1 1 1 1 1 1 n « 1 1 1 1 ■ j.j^g Reflex; B = Conditioned Reflex to

Irradiation in a Normal Fish; C = Con-
ditioned Reflex to Irradiation in a

I 1 I Fish that had its Brain Hemispheres Re-
moved; D = Absence of the Conditioned

»» • i Reflex to Irradiation in a Fish with a

Damaged Diencephalon; 1) Marking of Ir-
J. radiation; 2) Marking of the Electric

Stimulus; 3) Motor Reaction of the Fish;
V 4) Time Marking with an Interval of 1

. . ^ . . . 1 1 1 1 n iiiii i 1 1 1 iiii im I II 1 1 1 Sec.

164

merits was necessary because of questions concerning the direct effect of ir-
radiation on the brain hemispheres. Along with this series of tests, we an-
swered the question about the participation of the olfactory analysor in the
perception of t irradiation.

These tests were conducted on two carp. A morphological check showed
that the forebrain was completely removed. As is evident from the data of
Table 28, conditioned reflexes arose later in the operated fish than in normal
fish; however, the conditioned reflex developed clearly (Figure 65, C) and its
stability increased as the number of combinations increased, although during
200 combinations it did not attain the stability that characterizes normal fish.
These results testify that the forebrain does not play the basic role in per-
ception of Y irradiation.

We also decided to check how destruction of the diencephalon afffects these
conditioned reflexes in fish, since the literature contains many indications
of the important role of the diencephalon in reactions to radiation effects. In
carp no. 7 we could not develop a conditioned reflex to irradiation after des-
truction of the diencephalon (Figure 65, D) , although we gave 200 combinations
of the conditioned and unconditioned stimuli. After destruction of the dien-
cephalon in carp no. 8 the previously developed conditioned reflexes to y ir-
radiation disappeared and we could not restore them during 200 combinations.
Thus, destruction of the diencephalon completely precluded the possibility of
developing conditioned reflexes to y irradiation in fish.

10^/1"^^^ experiments conducted by A. B. Tsypin on rabbits [Tsypin and Kholodov,
iyb4J support the tests described above. Conditioned electrodefensive reflexes
to Y irradiation were developed in 10 adult rabbits. To exclude the effect of
the weak sound stimuli arising during switching of the cobalt source, 1-1.5
months before the start of the experiment bilateral destruction of the inner
ear was surgically performed on all rabbits. The conditioned stimulus was y
radiation in a dose of 0.5-0.1 R/sec. Electric stimulation of the hind legs /195
was used as reinforcement. The influence of the conditioned signal lasted 3-4
sec. The unconditioned stimulus was given with a lag of about 3 sec and lasted
about 0.5 sec. Five combinations were given each day. The motor reactions of
the rabbits were recorded with the aid of a special electric transducer.

Defensive motor conditioned reflexes to the Influence of y irradiation
occur in all test animals after 15-50 combinations. However, as in the tests
on fish, a stable reflex was not obtained. Furthermore, when the total absorb-
ed dose exceeded 150-200 R, the preformed conditioned reflex connection began
to weaken. This was evident in the fact that the number of positive responses
began to decrease gradually. If, however, the animal was given several days
of rest, the conditioned reflex activity improved again. Thus, the tests on
rabbits supported the supposition that ionizing radiation can exert an effect
as a weak stimulus.

The tests with extirpations showed that the distance exteroceptors (vision,
smell, hearing) do not play a large role in the perception of y irradiation.
This was proven with respect to vision and smell in direct experiments on fish
with removal of the corresponding analysors. As for hearing, direct experiments

165

were conducted with destruction of the middle ear in rabbits, and indirect data
were obtained in exoeriments with fish. The fact is that after destruction of
the diencephalon, conditioned reflexes to sound are retained in fish [Kholodov,
1959] The disappearance of conditioned reflexes to irradiation in fish after
destruction of the diencephalon shows that the possible accompanying auditory
stimuli do not play roles in the development of the reflex to irradiation and
that the auditory analysor has no importance in the process of radiation percep-
tion The basic role in the reactions to irradiation belongs to the diencepha-
lon. This conclusion is supported by additional facts. It turned out that
after the tests on development of conditioned reflexes to irradiation, the
coloring of the test fish became darker than that of control fish in conditions
of the same illumination. This phenomenon can be explained by the effect of
irradiation on the diencephalon where the coloration center is located in fish
[Puchkov, 1954].

Discussion

In presenting the material on the development of conditioned electrode-
fensive reflexes to penetrating factors in fish, we observed a similarity in
the effect of these stimuli. This similarity appears especially clearly in a
comparison of the average magnitudes of the conditioned reflexes to UHF and
constant magnetic fields, y irradiation, light, sound and tactile stimulation
(Table 29).

/196

TABLE 29. AVERAGE INDICES OF CONDITIONED-REFLEX
ACTIVITY TO DIFFERENT STIMULI IN NORMAL FISH.

Stimulus

X!
CO
•H

Number of combinations

Stabil-
ity, %

before the
appearance
of the re-

before the
fixation of
the reflex

flex

light
sound

8
8

4
3

90
80

tactile

76
45

Y irradiation

UHF field

magnetic field

The data of Table 29 show that with respect to all indices our stimuli are
divided into two groups. Conditioned reflexes are well developed to stimuli for
which specialized receptors are known (light, sound and the tactile stimulus),
but the reflexes developed poorly to stimuli for which receptors are not known.
Furthermore, responses to these penetrating stimuli were not fixed in all fish.

166

i.e., we did not manage to obtain five responses in a row in all fish.

Although it is difficult to compare the effects of stimuli of different
modalities, from the data of Table 29 it follows that, within the limits of
each of the two groups of stimuli, the differences between them are insignifi-
cant. We can assume that the electrodefensive method reveals only the most
general properties of the stimuli and that the two groups mentioned are reflect-
ed in this rough evaluation.

In the Pavlov school, the problem of the qualitative characteristics of
conditioned stimuli was stated in connection with a study of temperature con-
ditioned stimuli. The investigators working with this stimulus [Voskoboynikova-
Ganstrem, 1906; Solomonov, 1910; Shishlo, 1910; Vasil'yev, 1912] noted that the
temperature conditioned reflex was difficult to develop, soon disappeared in
spite of an increase in the number of combinations, and even began to reduce
the reaction to other conditioned stimuli. Dogs became drowsy and stopped res-
ponding to all conditioned stimuli. This phenomenon was called the soporific
reflex. Discussing the results of tests with temperature stimuli, in 1910, I.
P. Pavlov wrote: "It has become apparent that a definite agent of the external
world can bring about somnolence in an animal and extinction of its higher ner -/197
vous activity, in just the same lethal and unconditional manner as that by which
other agents cause certain complex-nerve functions. To put it differently, to-
gether with diverse active reflexes there are passive soporific reflexes".*

However, a qualitative uniqueness of temperature stimuli was cast into
doubt by the work of Rozhanskiy (1913) who, using special methods, developed a
stable conditioned reflex to a temperature stimulus. Although this did not
directly negate the qualitative difference of temperature stimuli from any other
stimuli, the difference in the effect of conditioned stimuli began to be ex-
plained from the point of view of their quantitative differences. "The differ-
ence in the magnitude of the effect of our ordinary conditioned stimuli which
refer to various analysors is caused by the difference in the strength of these
stimuli and is not connected with the qualities of the cells of different analy-
sors .**

Over the next 50 years, conditioned temperature reflexes were studied very
little. The well-known work of N, N. Dzidzishvili (1953) supports the conclu-
sions of earlier works of the Pavlov School about temperature conditioned
stimuli: "The unique flow of conditioned-reflex activity in response to tempera-
ture stimulations of moderate and high strength should be explained by the fact
that the temperature stimulus differs in its character from other exteroceptor
stimuli; as I. P. Pavlov affirmed, it is an agent that causes a well expressed
fatal inhibition".***

*I. P. Pavlov: Dvadtsatiletniy opyt ob" yektivnogo izucheniya vysdiey
nervnoy deyatel'nosti zhivotnykh. (Twenty Years' Experience in the Objective
Study of Higher Nervous Activity in Animals.) Moscow, 1951, p. 83.

**I. P. Pavlov: Lektsii o rabote bol'shikh polushariy golovnogo mozga.
(Lectures on the Work of the Cerebral Hemispheres.) Leningrad, 1927, p. 236.

***N. N. Dzidzishvili: Ob uslovnykh ref leksakh na teplovye razdrazheniya kozhi.
(Concerning Conditioned Reflexes to Thermal Stimulation of the Skin.) Trudy
Instituta Fiziologii AN Gruz. SSR. (Transactions of the Institute of Physiology
of the Georgian Academy of Sciences.) 9.: 94, 1953.

167

The predominantly inhibiting effect of a magnetic field observed during
development of conditioned stimulus in fish and pigeons makes this reflex ap-
proximate temperature conditioned stimuli. Like the temperature stimulx, a
magnetic field can be classified as a weak stimulus. However, we should note
that the predominantly inhibiting effect of electric and tactile stimuli [Yero-
feyeva, 1912; Podkopayev, 1932; V. K. Fedorov, 1954] is observed in the region
of threshold intensities, but the inhibiting effect of temperature and magnetic
stimuli is observed at intensities that greatly exceed threshold intensities.

The similarity of the magnetic stimulus and the temperature stimulus is
elicited not only in their predominantly inhibiting effect, but also in the
fact that their application increases the number of intersignal reactions, al
though this property of temperature stimuli has not been specially investigated.
The appearance of salivation in the intervals and the increase of somnolence ^lya
in dogs occurred in parallel in tests with temperature stimuli [Solomonov, 1910;
Shishlo, 1910]. Intermediate salivation is explained by the disinhibitmg in
fluence of the aftereffect from the temperature stimulus. Together with the
predominance of the inhibition process, the increase in the number of intersig^
nal reactions was noted during the influence of weak electric stimuli [Yerofey
eva, 1912], subsensory sound stimuli [Chistovich, 1949] and in tests with cover-
ing [Vinogradov, 1954] .

Studying the effect of stimuli of different strengths on the delayed con-
ditioned reflex, I. P. Pavlov showed that weak stimuli disinhibit the inert
phase of this reflex, stronger stimuli disinhibit the inert and inhibit the
active phase and, finally, strong stimuli inhibit the active phase of the delay-
ed reflex. The stimulus we analyzed probably should be classified among those
that disinhibit the inert and inhibit the active phase of the delayed reflex,
since both a magnetic field and a temperature stimulus inhibit the conditioned
reflexes developed to other stimuli and disinhibit the response to the situa-
tion, i.e., increase the number of intersignal reactions.

An unstable conditioned reflex can usually be developed to such weak
stimuli. In our tests with fish a relatively stable conditioned reflex to a
magnetic field was developed by the food-getting method and a less stable one
by the electrodefensive method. In the tests with pigeons we did not manage to
develop either a positive or an inhibiting conditioned reflex to a constant mag
netic field, and in the tests with rabbits our attempt to develop a positive
reflex was not successful. We propose that a magnetic field is a stronger stimu-
lus for fish than for pigeons or rabbits. In pigeons the effect of a constant
magnetic field is revealed only in induced inhibition of positive light-condi
tioned reflexes and in an increase in the number of intersignal reactions.

Consequently, under certain conditions the stimulus can be perceived, but
its strength will be insufficient for the formation of a conditioned reflex. In
other words, the receptor possibilities of an animal can be determined most pre
cisely not by development of a conditioned reflex to the investigated stimulus,
but by a study of its effect on previously formed conditioned reflexes. The et
feet on animals of a UHF field [Livshits, 1958], ionizing radiations [Lebedinskiy ,
1955], and stimulations of different internal organs [Bulygin, 1952J has been
studied by this means.

168

Stimulations that by themselves do not cause reflex activity have been call- / 19 9
ed correcting stimuli, in contrast to triggering stimuli. It seems to us that
the effect of a magnetic field on the behavior of pigeons can be an example of
the effect of a correcting stimulus. The correcting effect has been studied
most thoroughly by N. A. Bulygin, who "established that during stimulation of
the interoceptors the correcting effects precede triggering effects and contin-
ii§. after them so that they can be observed by themselves , without triggering ef-
fects, (the underline is mine — Yu. Kh.). while the latter are always accompani-
ed by the former and, consequently, the triggering effects from interoceptors
to the motor and salivary centers are only a higher stage of the correcting ef-
fects".*

It is probable that in pigeons we managed to see only the correcting ef-
fect of a magnetic field, but in fish the magnetic field could also cause a
triggering effect, i.e., become a conditioned stimulus.

The tests in which we studied the effect of a CMF on the reactance curve
in rabbits (see Part I) also demonstrate the presence of a directing effect of
this factor on mammals. From this we can assume that a CMF will have an effect
on developed conditioned reflexes in mammals. Having become a conditioned stimu-
lus for fish, the magnetic field revealed its correcting effect in an increase
in the number of intersignal reactions and in a predominantly inhibiting effect
rather sharply in comparison with other stimuli.

In our opinion, the results of all these tests give a sufficiently valid
affirmative answer to the question about whether a magnetic field is a stimulus
for an organism. By the conditioned-reflex method it was observed that a mag-
netic field is perceived by fish and pigeons, but its correcting effects are
projected more clearly than its triggering effects.

Conclusions

1. We did not manage to develop a positive defensive conditioned reflex

in rabbits or a food-getting conditioned reflex in pigeons to a CMF. The effect
of a CMF on pigeons was revealed in an increase in the number of intersignal
reactions and in inhibition of a previously developed light conditioned reflex.

2. In fish we managed to develop positive electrodefensive conditioned /200
reflexes to a magnetic field, a UHF field and y irradiation. The developed
reflexes appeared later and were less stable than the conditioned reflexes to
light or sound. Inhibiting reflexes to a magnetic field were developed better
than to light or sound. The effect of the magnetic field on fish was also ex-
pressed in an increase of the number of intersignal reactions and in inhibition

of previously developed light- and sound-conditioned reflexes.

*N. A. Bulygin: zakonomernosti i mekhanizmakh vliyaniy s interroretsepto-
rov na reflektornuyu deyatel'nost ' spinnogo i golovnogo mozga. (Concerning the
Regularities and the Mechanisms of Effects from the Interoceptors on the Reflex
Activity of the Spinal Cord and the Brain.) Doctoral Dissertation, Leningrad,
1952, p. 19.

169

3. The threshold of perception of a magnetic field by fish during develop-
ment of a positive electrodefenslve conditioned reflex was 10-30 Oe.

4. From the character of their effect, a magnetic field, a UHF field and
Y irradiation can be classified as weak correcting stimuli.

170

CHAPTER 6. ANALYSIS OF THE MECHANISM OF THE FORMATION OF CON-
DITIONED REFLEXES TO A MAGNETIC FIELD IN FISH

The fact that we did not manage to develop conditioned reflexes to a CMF in
rabbits allows us to assume that this physical factor somehow acts differently
on fish than on mammals. Since the basic data about the mechanism of the effect
of a CMF was obtained on rabbits through the use of the electrophysiological
method, this assumption forced us to conduct a special analysis of the mechanism
of perception of a CMF in fish. In this series we investigated the roles of the
lateral line organ, the retina, and also different structures of the brain in the
formation of conditioned reflexes to a CMF in fish. Most of the experiments
were conducted by the electrodefensive method.

The Effect of Denervation of the Lateral Line Organ on the
Conditioned Magnetic Reflex in Fish

Lissman (1958) assumed that a CMF acts on fish by induction of an electro-
motive force and that the basic role in perception of this electromotive force
is played by the lateral line organ. Although it is known that the basic func-
tion of this organ in fish is the perception of infrasonic oscillations
[Malyukina, 1955], we can assume that the perception of a magnetic field is
among the additional functions of this organ. For an experimental check of this/201
assumption we denervated the lateral line organ in goldfish no. 1 and 2 and in
carp no. 11.

BO
VO
20

(a)

33 36 JJ 3S il 13

Figure 66. Dynamics of the
Food-Getting Conditioned
Reflex to a Magnetic Field
in Fish After Partial De-
nervation of the Lateral
Line Organ. The Arrow
Notes the Moment of the
Operation. Key: (a) Reac-
tion Stability, %; (b) Num-
ber of the Test.

On Figure 66 one can see that bilateral tran-
section of n. lateralis, which was conducted af-
ter Test No. 39 on goldfish no. 2, absolutely did
not disturb the stability of the conditioned re-
flex to a magnetic field. Large variations in re-
flex stability are observed both before the opera-
tion and after it. In goldfish no. 1 we checked
the stability of the conditioned reflex to a mag-
netic field on the day of the operation. The re-
flex did not change. In carp no. 11 we denervated
the lateral line organ after bilateral enuclea-
tion. During the operation we performed bilateral
transection of n. lateralis and n. suborbitalis.
A test of the stability of the conditioned reflex
to a magnetic field conducted on the same day
showed that a response was observed in 9 out of
10 applications of the magnet.

Consequently, denervation of the lateral line
did affect perception of a magnetic field by fish.
Separate observations show that denervation of the
lateral line organ also does not change the con-
ditioned light reflex. Of course, these tests do

171

not answer the question about the participation of the integxjment in perception
of a magnetic field. However, the difficulty of fully precluding the skin ana-
lysor forced us to turn our attention to a determination of the central link in
the reflex arc for the reaction of fish to a magnetic field.

Similarity in the Effects of Light and a Magnetic Field on Fish

Having decided to look for the anatomic locxis of the conditioned reflex to
a magnetic field, we began to develop in our fish, in addition to the existing
conditioned magnetic reflex, conditioned reflexes to the flash of an electric
light and the sound of an electric bell. These conditioned reflexes served as
the control during various surgical procedures.

Before the operations we determined the effect of a magnetic conditioned
reflex on the light conditioned reflex. In goldfish no. 1, 2 and 3, in which a
conditioned reflex to a magnetic field had already been developed, the reflex
light appeared and became fixed during the first application in goldfish no.
1 and 2, and it appeared at the 3rd combination and became fixed after the 6th
combination in goldfish no. 3 (Table 30).

7202

TABLE 30. CHARACTERISTICS OF THE FOOD-GETTING AND ELECTRODEFENSIVE
POSITIVE CONDITIONED REFLEXES TO LIGHT AND SOUND DEVELOPED IN FISH
AFTER THE FORMATION OF A CONDITIONED REFLEX TO A MAGNETIC FIELD.

Light

Bell

Number of combinations

Stabil-
ity. %

Number of combinations

Stabil-
ity, %

Fish
No.

Before ap-
pearance
of the
reflex

Before
fixation
of the
reflex

Before ap-
pearance
of the
reflex

Before
fixation
of the
reflex

1
2
3

1
1
3

i
1
1
6

:ood-getting
65
72
63

method

39
27

55
72
48

51
88
89
90

7
1
1
1

elei
14
1

2trodefensi\

100

re method
2
4
1

29
15

7
1

70
82
85
79

In comparison with Table 24, which gives the characteristics of the posi-
tive conditioned reflex to light in goldfish in which time relationships had not
been previously developed, the data of Table 30 indicate not only the rapid ap-
pearance of the light reflex, but also its greater stability. The average sta-
bility of the light conditioned reflex for goldfish in Table 24 was 60%, and in

172

Table 30, 67%. These regularities are revealed even more sharply in carp through
the use of the electrodefensive method. Here the stability of the light reflex
exceeds 95% in comparison with a stability of 56% (Table 23) when the light re-
flex was developed first.

The clear generalization of the reflex during application of the light
stimulus cannot be explained only by the fact that the reflex to light was de-
veloped after the conditioned reflex to the magnetic field. For example, al-
though the conditioned reflex to the bell was developed after the reflexes to
the magnetic field and to light were fixed, the reflex to the bell does not show
such a degree of generalization. Under ordinary conditions, as we have stated
before, the reflex to light and to the bell are formed at an identical rate
(Table 23).

We assumed that the presence of a conditioned reflex to a magnetic field
accelerates subsequent development of a conditioned reflex to light and we con-
ducted special tests to check this assumption. In fish no. 11 and 12 we devel-
oped a conditioned reflex to a magnetic field, and once in the test we applied
light without reinforcement. At first neither the magnet nor the light caused
the conditioned response, but as soon as the conditioned reflex to the magnetic
field became fixed, the response also appeared to the application of light. To
illustrate this phenomenon we give the record, of Test No. 9 with carp no. 12.

/203

TEST NO. 9. CARP NO. 12, 21 DECEMBER 1954.

Stimulus

Time of switching

S timulus

Conditioned

Unconditioned

number

on the stimulus

response

reinforcement

11 hr 13 min 00 sec

magnet

11 hr 15 min 00 sec

11 hr 17 min 00 sec

—

11 hr 19 min 30 sec

11 hr 23 min 00 sec

11 hr 25 min 15 sec

light

—

11 hr 28 min 30 sec

magnet

11 hr 32 min 00 sec

11 hr 35 min 00 sec

11 hr 38 min 00 sec

11 hr 41 min 00 sec

11 hr 43 min 00 sec

bell

—

The response to light observed during development of the conditioned reflex to
a magnetic field cannot be explained by the general increase in excitability of
the nervous system, since the application of the bell in the same test did not
cause the response. It is Interesting to note that the response to light was
observed only at a definite degree of stability of the conditioned reflex to the
magnetic field. When the stability was low, there was no response to light.

173

TEST NO. 11. CARP NO. 14, 25 DECEM-
BER 1954. THE TEST BEGAN AT 11:29.

S tlmulus

Time of switching

Stimulus

Conditioned

Unconditioned

number

on the stimulus

response

reinforcement

111

11 hr 29 min

light

—

112

11 hr 32 min

113

11 hr 38 min

114

11 hr 42 min

—

115

11 hr 44 min

—

11 hr 46 min

magnet

"""

116

11 hr 48 min

light

—

117

11 hr 50 min

118

11 hr 52 min

119

11 hr 54 min

120

11 hr 56 min

—

11 hr 59 min

bell

—

"■"

In carp uo. 14 we developed a conditioned reflex to light, and sometimes
we applied a magnetic field without reinforcement. The obtained results are
well illustrated by the introduced test record.

The test results show that during development of the conditioned reflex to
light we observe a reaction to a magnetic field the application of which was
never accompanied by an electric shock. At the same time the application of the
bell did not cause a response. Based on this material we can conclude the pres-/204
ence of a definite similarity in the physiological effects of light and a magne-
tic field.

Retention of the Conditioned Magnetic Reflex in Fish After

Enucleation

Since the mechanism of the effect of light on animals is well known, we de-
cided that a magnetic field is perceived just as light is, i.e., by the retina.
This position was supported by data in the literature concerning the possibility
of producing the sensation of phosphene during the influence of a magnetic field
on man [Mogendovich and Skachedub, 1957]. It is natural, therefore, that the
next stage in our tests was a check on the retention of the conditioned reflex
to a magnetic field and the possibilities of its development after bilateral
enucleation.

In carp no. 11 and 12, in which we had developed the conditioned reflex to
a magnetic field, we disturbed the visual receptor by destroying the retina
(carp no. 12) or sectioning the optic nerves (carp no. 11). The tests began on
the day of the operation or on the next day.

174

too

80
it, BO

t vo

S 20

too

^60

(a)

vo ■

7 d 9 to II 12
(b) ^O'^^P on mm a

Figure 67. Dynamics of the
Conditioned Electrodefen-
sive Reflex to a Magnetic
Field in Fish After Bilat-
eral Enucleation. A = Carp
No. 11; B = Carp No. 12.
The Arrows Designate the
Moment of the Operation.
Key: (a) Reaction Stabil-
ity, %; (b) Number of the
Test.

lag period; thus the remaining
the retina.

As Figure 67 shows, after destruction of the
visual receptor the conditioned reflex to a mag-
netic field did not disappear, but even increased / 205
somewhat. The results obtained put the hypothe-
sis about the similar effects of light and a mag-
netic field on the retina in doubt. Therefore,
we assume that light and a magnetic field can be
perceived in fish not only by the retina. This
assimiption was based on the data about the possi-
bility of fish perceiving light with the aid of
the skin analysor [Parker, 1905] or directly by
the diencephalon [Scharrer, 1928; Jung, 1935].

We began to develop conditioned reflexes in
the blinded fish 1-10 days after the operation.
Table 31 gives the test results on the develop-
ment of conditioned reflexes to white light in 7
fish after blinding.

From the data of Table 31 it follows that
the rate of formation of the conditioned reflex
to light in blinded fish differs little from the
corresponding index for fish with vision, but the
reflex stability is lower in the blinded fish.

The test results clearly show that light
acts on fish not only through the eye, but through
other means whose activity can be successfully
studied by the method of conditioned reflexes.
A less stable conditioned reflex is formed to the
light that is perceived by the receptor left in
fish after enucleation; it appears with a longer
photoreceptors perceive light less perfectly than

After the possibility of light perception by blinded fish had been affirmed
[Kholodov, 1958a) , we decided to find out how the general nature of the percep-
tion of light and a magnetic field changes in enucleated fish.

In 3 blinded carp (no. 1, 2 and 3) we simultaneously developed conditioned
reflexes to light and to a magnetic field, alternating the stimuli in the test,
and in 2 blinded carp (no. 4 and 5) we developed the reflex to light and check- /206
ed its generalization for a magnetic field. The results of simultaneous devel-
opment of conditioned reflexes to light and to a magnetic field are given in
Table 32 and Figure 68.

The data of Table 32 show; that the conditioned reflexes to light and to
the magnetic field appear simultaneously, but the reflexes to light are fixed
sooner. At the same time in the fish with vision the conditioned reflex to
light appeared and was fixed much sooner than the reflex to the magnet. Conse-
quently, the similarity in the effects of light and a magnetic field was increas-

175

TABLE 31. CHARACTERISTICS OF THE CONDI-
TIONED REFLEXES TO LIGHT IN BLINDED FISH.

Number of Combinations

Lag peri-

Stabil-

Before ap-

Before

Fish No.

pearance
of the

fixation
of the

od, sec

ity, %

reflex

6.1

5.3

4.0

5.7

4.9

8.0

7.2

Averages

7.4

16.0

5.9

49.0

ed in the blinded fish. This similarity can be seen more completely in Figure
68, where the curves of the stability of the conditioned reflex to a magnet al-
most completely duplicate the curves of the stability of the conditioned reflex
to light for each fish.

TABLE 32. RATE OF DEVELOPMENT OF CONDITIONED REFLEX-
ES TO LIGHT AND TO A MAGNETIC FIELD IN BLINDED CARP.

Fish No.

Light

Magnet

Number of combinations

Before ap-
pearance
of the
reflex

Before
fixation
of the
reflex

Before ap-
pearance
of the
reflex

Before
fixation
of the
reflex

1
2
3

64
48
38

We can illustrate the generalization of the conditioned light reflex under /207
the effect of the magnetic field by the record of Test No. 6 on fish no. 49.

From the record it follows that generalization of the light conditioned re-
flex under the effect of a magnetic field in blinded fish appears very clearly.
Similar results were obtained in tests with carp no. 48. The generality of the

176

TEST NO. 6. CARP NO. 49, 15 JUNE 1955. THE TEST WAS BEGUN AT 10:00.

S timulus

Time of

switching

Stimulus

Conditioned

Unconditioned

number

on the

stimulus

response

reinforcement

10 hr 00

min 00 sec

light

10 hr

min sec

—

10 hr

min sec

—

10 hr

min sec

—

10 hr

min sec

10 hr

min sec

magnet

— M

10 hr

min sec

light

—

10 hr

min sec

—

10 hr

min sec

—

10 hr

min sec

—

10 hr

min sec

—

10 hr

min sec

magnet

too

^jf — -Ik

y='

■

' \ 1 1

\/-^^

1
1

1 \ 1 1

/ 2 3 H 5 6 7 8 9 10 11 1Z

/ Z J 1 5 6 7 8 9 10 It 12
(b) HOMPP onbimn

Figure 68. Dynamics of the Stability of Electrodef ensive
Conditioned Reflexea to a Magnetic . Field (1) and to Light
(2) in Blinded Carp.. A = Carp No. 1; B = Carp No. 2; C =
Carp No. 3. Key: (a) Reaction Stability, %; (b) Number
of the Test.

177

effect of light and a magnetic field after blinding of fish, not only did not de-
crease, but even increased. Therefore, the idea of a similar mechanism for the
pffppi- of Moht- anH a m=>o'netic field remains dominant in our later tests.

The Role of Different Sections of the Fish Brain in the Reali- /208.
zation of a_ Conditioned Electrodefensive Reflex to a Magnetic

Field

The localization of the fixing function in the fish brain has not been
sufficiently studied. Since this class of vertebrates lacks a cortex, certain
investigators have assumed that fixing is accomplished in fish in the midbrain
and the diencephalon [Beritov, 1932; Frolov, 1941; Bykov, 1952]. Based on the
results of other works we can conclude that the fixing of conditioned reflexes
in fish occurs in the cerebellum [Karamyan, 1949, 1956; Malyukina, 1955].

We decided to clarify the role of the 4 basic sections of the fish brain
(midbrain, diencephalon, visual tegmenta and cerebellum) in the realization of
the conditioned defensive reflex to a magnetic field. As a control we developed
conditioned reflexes to light and to a bell. In one test we usually gave 10 com-
binations of the magnet, 3 combinations of the light and 3 combinations of the
bell with the unconditioned electric stimulus.

Development of Conditioned Reflexes After Damage to the

Visual Tegmenta

TABLE 33. SUMMARIZED DATA ON FISH
AFTER DAMAGE TO THE VISUAL TEGMENTA.

Date of

Date the

Day the

Date of

Survival

Fish

the op-

tests

reflex to

Number

death.

after op-

No.

erations.

started,

the CMF

of tests

1955

eration.

1955

appeared

in days

Jan. 25

Jan. 28

Feb. 7

Jan. 25

Jan. 28

Feb. 8

Jan. 25

Jan. 28

Feb. 18

Mar. 1

Mar. 7

Mar. 10

Mar. 14

Mar. 29

Apr. 8

Mar. 7,
1956

Mar. 20,
1956

Mar. 31,
1956

We began the tests on extirpation of separate parts of the fish brain with

removal of the visual tegmenta since this section of the brain takes part in the

effectuation of the conditioned response to light [Baru, 1955], whose effect is ^209
similar to that of a magnetic field.

Conditioned reflexes were developed in 6 carp after removal of the visual

178

tegmenta. Table 33 gives the sunmarized data on the operated fish.

The data of Table 33 show that regardless of the length of the postop-
erative period, which varied from 3 to 15 days, reflexes to the magnetic field
are formed on the 1st or 2nd day after the beginning of the tests. It is char-
acteristic that the survival of the fish after the operation was brief (from 10
to 25 days) and that all the fish died. We explain this by the fact that either
during the operation or, more likely, because of the inflammation forming after
the operation, the functions of the lower-lying sections of the brain were dis-
turbed.

The results of the tests on development of conditioned reflexes in the op-
erated fish are given in Table 34.

TABLE 34. CHARACTERISTICS OF THE CONDITIONED REFLEXES TO A MAGNETIC
FIELD, LIGHT AND A BELL IN FISH AFTER REMOVAL OF THE VISUAL TEGMENTA.

Magnet

Light

Bell

Number of

combinations

Before

appear-
ance of

fixa-
tion

•rl

appear-
ance of

fixa-
tion

•H

appear-
ance of

fixa-
tion

•iH

the re-

of the

■f?l

the re-

of the

•H

the re-

of the

flex

reflex

■U

flex

reflex

CO
U
Cfl

flex

reflex

CO
4-1

—

We managed to develop a conditioned reflex to a magnetic field in all fish. / 210
although only 3-9 tests were conducted with each fish. The average stability of
the conditioned reflex to the magnetic field (43%) somewhat exceeded the same
index for intact fish.

We did not develop conditioned reflexes to other stimuli in all fish. Con-
ditioned reflexes to light and to the bell were developed in 4 carp (no. 1, 2,
3 and 6) . The stability of the conditioned light and sound reflexes was less
than that in intact fish. We explained the appearance of a conditioned reflex
to these stimuli during their first application, which was sometimes observed,
by generalization since both the light and the bell were applied in the test af-
ter the magnetic field. The possibility of developing conditioned reflexes af-
ter removal of the visual tegmenta is illustrated by the kjnnograms of the tests
(Figure 69).

179

i.-JUi-W

*— ««L.

/%7

^fl

/V//

-M S^

■A — i>*-

*mV.

■V-

Figure 69. Kymograms of Tests on the Development of
Electrodefensive Conditioned Reflexes to a Magnetic
Field and to Light in Fish After Damage to the Visual
Tegmenta. The Designations Are the Same as in Figure
61. Key: (a) Magnetic Field; (b) Light.

In one carp we destroyed the visual tegmenta after we had developed the con-
ditioned reflex to the magnetic field. It turned out that the reaction to the
magnetic field was retained. Thus, destruction of the visual tegmenta did not
change the conditioned reflex to a magnetic field and reduced the stability of
conditioned reflexes to light and to a bell.

Development of Conditioned Reflexes After Removal of the

Cerebellum

Tests on the development of conditioned reflexes were conducted on 7 carp
(Table 35).

TABLE 35. SUMMARIZED DATA ON FISH AFTER
COMPLETE REMOVAL OF THE CEREBELLUM.

Starting

Day the
reflex to

Date

Lifetime.

Fish

Date of

date of

NumDer

No.

operation

tests

a CMF ap-
peared

of tests

of
death

of sac-
rifice

in days

[1955]

Apr. 20

May 16

[1955]

May 27

June 7

June 14

June 20

—

June 7

June 14
[1956]

Jun$ 17
[1956]

Dec. 30
[1956]

Jan. 16

Jan. 20

[1956]

Mar. 7

Mar. 20

—

Apr. 4

Mar. 7

Mar. 20

—

Apr. 4

May 16

May 26

May 29

180

The conditioned reflex to a magnetic field appeared in all fish. We ob-
tained the most complete picture of the conditioned-reflex activity in the case /211
of 3 fish (no. 1, 5 and 6) that lived for more than 25 days after the operation
and that were sacrificed for a morphological check of the completeness of re-
moval of the cerebellum.

The characteristics of the conditioned reflexes of all fish are given in
Table 36.

TABLE 36. CHARACTERISTICS OF CONDTIONED REFLEXES TO A MAGNETIC
FIELD, LIGHT AND A BELL IN FISH AFTER REMOVAL OF THE CEREBELLUM.

Magnet

Light

Bell

Number of

combinations

Before

Before •

appear-

fixa-

4-1

appear-

fixa-

•H

appear-

fixa-

■iH

ance of

tion

.-1
■r-l

ance of

tion

ance of

tion

•H

■H

the re-

of the

-S

the re-

of the

■^

the re-

of the

•^

flex

reflex

flex

reflex

■U

flex

reflex

•U

—

MF
Ma

"XJ-

-« *—

L
CB

■^^

1 —

Figure 70. Kymograms of Tests on Developing Electrodefensive Condition-
ed Reflexes to a Magnetic Field, Light and Sound in Fish After Removal
of the Cerebellum. A = Fish No. 5; B = Fish No. 6; MF = Magnetic Field;
L = Light; S = Sound. The Remaining Designations Are the Same as in Fig-
ure 61.

181

In fish which lived long enough after the operation, we managed to develop
a conditioned reflex to all stimuli. In the fish that died soon after the op-
eration we observed the appearance of conditioned reflexes to all stimuli. The
stability of the conditioned reflexes shows that after removal of the cerebellum
the conditioned reflex to the bell is disturbed most severely. The stability
was not calculated for certain fish because the number of applications of the
conditioned stimulus was not great enough. The possibility of developing con-
ditioned reflexes in fish after removal of the cerebellum is illustrated by the
kymograms of Figure 70.

We assume that the noncorrespondence of our results with the data of A. I.
Karamyan can be explained by the low age of our fish (1-2 years) and their bet-
ter survival rate after the operation, which reduced the effect of postoperative
disturbances on the development of conditioned reflexes. The significant dis-
turbance in the conditioned sound reflex after removal of the cerebellum may
either be explained by the fact that sound reflexes are fixed in the cerebellum
[Malyukina, 1955] or by the fact that the conducting paths of this reflex are
tightly connected with the cerebellum [Kappers et al., 1936]. The second state-
ment seems more probable to us.

Development of Conditioned Reflexes After Removal of the Forebrain

Although many works deny the effect of the forebrain on conditioned reflex-
es to light and sound in fish [Baru, 1955; Healey, 1957], we decided to try to
develop a conditioned reflex to a magnetic field after removal of the forebrain
from fish. The summarized data on 5 carp used in these tests are given in Table
37.

TABLE 37. SUMMARIZED DATA ON FISH AFTER REMOVAL OF THE FOREBRAIN.

/213

Fish
No.

Date of
operation

Starting
date of
tests

Day the
reflex to
a CMF ap-
peared

Number
of tests

Date of
sacrifice

1
2
3
4

Jan. 17, 1955
Jan. 17, 1955
Feb. 28, 1955
Apr. 28, 1956
Nov. 9, 1956

Jan. 24, 1955
Jan. 24, 1955
Mar. 29, 1955
May 23, 1956
Nov. 29, 1956

1
2
1
1
3

8
10

7
10
20

Feb. 21, 1955
Feb. 20, 1955
May 24, 1955
June 4, 1956
Mar. 24, 1957

The behavior of the fish changes little after removal of the forebrain.
Reflexes to the magnetic field are developed in the first through the third test.
Not one fish died.

The results of developing conditioned reflexes, given in Table 38, indicate
that the higher nervous activity of the fish did not significantly change.

182

TABLE 38. CHARACTERISTICS OF CONDITIONED REFLEXES TO A MAGNETIC
FIELD, LIGHT AND A BELL AFTER REMOVAL OF THE FOREBRAIN IN FISH.

Magnet

Light

Bell

Number of
combinations

6-S

4-1
•i-l
rH
•H

Number of
combinations

•H
.H

•rl

4-1
CO

Number of
combinations

•H

Before
appear-
ance of
the re-
flex

Before
fixa-
tion
of the
reflex

Before
appear-
ance of
the re-
flex

Before
fixa-
tion
of the
reflex

Before
appear-
ance of
the re-
flex

Before
fixa-
tion
of the
reflex

u
■H

r-l
•H

c«

1
2
3
4
5

23
26
2
11
16

108

50
25
60
35
24

1
8
4
4
1

9
4
3

60
30
97

1
1
1
6
7

1
10
19

91
85
54

The rate of development of the conditioned reflexes to the applied stimuli /214
and their stability clearly show that, if we do not count a certain decrease in
the stability of the conditioned light reflex after the operation, a fish with-
out a forebrain differs little from an intact fish. Nevertheless, as a control,
on carp no. 13, 15 and 20 we checked the retention of the conditioned reflexes
after removal of the forebrain. After removal of the forebrain the developed
reflex to a magnetic field in fish did not change.

Development of Conditioned Reflexes After Damage to the Diencephalon

The role of the diencephalon in the behavior of fish remains less known.
The existing works on extirpation of the diencephalon concern the effect of this
section of the brain on motion [Steiner, 1888] and respiration [Springer, 1928]
of Selachii.

TABLE 39. SUMMARIZED DATA ON FISH AFTER
DAMAGE TO THE FOREBRAIN AND DIENCEPHALON.

Starting

Day the

Fish

Date of

date of

reflex to

Number

Date of

No.

operation

tests

a CMF ap-

of tests

sacrifice

peared

[1955]

Jan. 12

Mar. 21

Feb. 19

Mar. 21

Feb. 19

Mar. 21

Apr. 28, 1956

May 15, 1956

June 4, 1956

Nov. 9, 1955

Dec. 29, 1955

Mar. 23, 1956

Dec. 30, 1955

Feb. 11, 1956

Mar. 23, 1956

183

We conducted tests on developing conditioned reflexes in carp, the summariz-

aA (i3tia of v/liich. are

given in Tabic 39.

We see that the conditioned reflex to a magnetic field either appears very
late or does not appear at all (carp no. 3), although the fish withstood the
operation very well and they all survived. Table 40, which gives the character-
istics of the conditioned reflexes for operated fish, shows significant dxstur-
bances of the conditioned reflex to the magnetic field. In fish no. 3 we did
not manage to form the conditioned reflex to the magnetic field in spite of 249
combinations of exposure to the magnetic field with an electric shock. In
fish no. 4, 5 and 6, although the conditioned reflex to the magnetic field ap-
peared, we could not fix it. In the fish in which fixation of the reflex oc-
curred (no. 1 and 2), it was less stable than similar reflexes of intact fish
or fish with other types of brain damage.

/215

TABLE 40. CHARACTERISTICS OF CONDITIONED REFLEXES
DEVELOPED IN FISH AFTER DAMAGE TO THE DIENCEPHALON.

Magnet

Light

Bell

Number of

combinations

B-S

•

Before

appear-

fixa-

•H

appear-

fixa-

•H

appear-

fixa-

•rl

CO
.pi

ance of

tion

i-l
•H

ance of

tion

•rl

ance of

tion

•rl
4J

the re-

of the

•S

the re-

of the

■^

the re-

of the

flex

reflex

4-1
CO

flex

reflex

flex

reflex

173

* ? = designates absence of fixation of the conditioned reflex.

The conditioned reflex to light was developed more poorly than in intact
fish or in fish after extirpation of other sections of the brain, but it was
still better than the conditioned reflex to the magnetic field.

The conditioned reflex to the bell was developed in all operated fish and
it differed little in its properties from the sound reflexes of intact fish.
The test results are illustrated by the kymograms of Figure 71. Consequently,
damage to the diencephalon leads to disturbances in the development of condi- lALL
tioned reflexes to the magnetic field and to light, but it changes the develop-
ment of the conditioned reflex to the bell very little.

The tests with damage to the diencephalon of blinded fish, in which condi-

184

X3^

^55

^^^

^ 'a ■

•vj

0)
1 X!

•H -W

C IW

o o

0) cd

> >

•rl O

•

to a

4-1

C! OJ XI

0) PiJ

•H

(U w

<-i

ID 0)

o -w

M m

J-> <3

<U x!

• #»

iH 03

W -H

•rt

O fl

•H

4-1

(3 -a

•H

01 C

4-1

6 3

ft o

. o CO

0) 13

> fi

0) «

p£j

0) X!

X! 60

•

■P -H

• »>

<-l

d^'S

O "

I-l

CO ^

c«

4-) Q)

M -H

•H

(U P^

0) 'H

•H

J3 u

•

U 0)

i:!

>w MiH

o to

a XI

a cd

4)
O

n o

60 4J

•H

>? S

fed X "O

• 4-1

r-i 0)

r- Pi

•H

0) T3

V4 <u

t :3 ti

f. 60 O

•r4 -H

•H

fl4 U

tioned reflexes to light and to a magnetic
field were preliminarily developed, show
that the conditioned reflexes to the magne-
tic field and to light disappear after the
operation.

Discussion

To clarify the general picture of the
change in conditioned reflex activity of
fish during different disturbances of the
central nervous system, we composed summary
Table 41.

In the future our conclusions will be
based on the average magnitudes, which, of
course, only approximately characterize the
quantitative side of the conditioned respon-
ses, since the number of cases for which
we calculated the averages was not great.
Furthermore, in calculating the averages
we disregarded the cases in which we did
not manage to obtain a conditioned reflex,
although these cases would be more correct-
ly expressed by very large magnitudes (in
the table they are designated by «>) .

For a more detailed analysis of the re-
sults obtained we composed diagrams illus-
trating the effect of damage to different
sections of the fish brain on separate in-
dices that characterize the conditioned re-
flex. The character of the disturbances in
the fish brain are noted on the abscissa,
and the absolute or relative magnitude of
the corresponding index of the conditioned
reflex is shown on the ordinate. The rate
of appearance, rate of fixation and the
stability of the conditioned reflex enter
into a ntmiber of these indices.

Figure 72, A shows the change in the ab-
solute magnitude for the rate of appearance of the conditioned reflex to a magne-
tic field, light and a bell for different types of brain damage. The greatest
disturbance of the conditioned reflex to a magnetic field and to light is observ-
ed after damage to the diencephalon. The reflexes to light and to the magnetic
field change little after damage to the other sections of the brain. The condi-
tioned reflex to the bell is disturbed after damage to the visual tegmenta and
the cerebellum, but it changes little after damage to the forebrain and dienceph-
alon. After almost all types of brain damage the conditioned reflex to the mag-
netic field appears later than the reflexes to the other stimuli, and the rates

185

Damage to the:
dlencephalon

TTsq

1-14
(6)

^■+

0\ /"^
c^^

35-71
(6)

^^^T^.

0^ z*^
1 ^

00
CM

aau
-Sbui

o >-^

O
CM

CM /^
CM vO
1 ^
O

rH
rH

-B
a

•H
H to

ta u
> ^

0)
fl) o

Tiaq

1 "^

0^ z*^.

rH n

O
rH

O
00

^qs-ti

00 /-^

1 "^

1 ^

5au
-Sbui

1 ^

3 "^

lU
XI
4J

<4-i e
o 3

rH
H rH

rt 0)
> XI

O 0)
U
(U (U
Pi o

TT^q

8 C-
1 ^

CO N--'
(M

CM ^-N

CO CO

1 ^^

3qSTT

rH 1^

1 ^-'

•<^

ff CO

vi-
vo

5au
-SBm

CM r^
1 ^-^

00 ^-N

P- CO

Damage to the
visual tegmenta

Tiaq

tN •*

1 ^

1^
CM

^qSTT

o --^

ro CO

1 ^

rH
rH

aau
-8bui

rH VD
CO

r--

CO ^-N

CM VO
1 ^
CO

Tisq

rH (30

0^ ^-N

^^^JT

rH /-N

CM 00

O ^
CO 00

1 ^

CM
H

aau
-3Bra

CM 0>

1 rH

CM v-'

O ^^
rH m

Conditioned
response

aSuBj

aSsaaAB

aSuBa

a3BaaAB

aSuBJ

aSBaaAB

-UIOO J

■uoo aqn
-jBaddB

uoT:jBu-i:q

jaqranu

pauo-f^-pp

JO aouB

JO a^BH

-moD J

'xaxjaj

-«oo aq3

-Bx-pj

uo-p^Bu-pq

D aaqumu

pauoja-pp

JO uo-p3

JO aasy

pauo-p:iT

JO X

•xaxjaJ
puoo aii3
^TTTq^^S

« /218

(11 ••

186

Vm Inn

(b)|| wo

-la

Figure 72. Absolute (A) and Relative (B) Changes in
the Rate of Appearance of Electrodefensive Condition-
ed Reflexes to a Magnetic Field (1) , Light (2) and
Sound (3) in Fish in the Norm (I) and After Damage
to the Forebrain (II) , Diencephalon (III) , Midbrain
(IV) or Cerebellum (V). Key: (a) Number of Applica-
tions of the Conditioned Stimulus; (b) Rate of Appear-
ance of the Conditioned Reflexes, %.

of appearance of the conditioned reflexes to light and to the bell in the normal
fish and after removal of the forebrain are identical, which is again supported
by the data of Table 23. It is interesting to note the coincidence in form of
the diagrams for the rate of appearance of the conditioned reflexes to light and
to the magnetic field. This similarity resembles the graph for the development
of conditioned reflexes to light and to a magnetic field in blinded fish and
again forces us to assume a generality of the action mechanisms of light and a /219
magnetic field. In order to judge the degree of change in the rate of appear-
ance of conditioned reflexes to different stimuli after different types of brain
damage, in Figure 72, B we show these changes in percentages, taking the rate of
appearance of the reflexes in the norm as 100%. We see that the degree of dis-
turbance in the rate of appearance of the conditioned reflexes is greatest in
the reflex to the magnetic field (by 350%), then comes the bell (by 300%) and,
finally, light (by 275%).

Figure 73, A shows the change in the absolute magnitude of the rate of
fixation of conditioned reflexes to a magnetic field, light and a bell after
different types of damage to the fish brain. As in Figure 72, the greatest dis-
turbance in the conditioned reflex to a magnetic field and to light is observed
after damage to the diencephalon, and in the reflex to the bell, after damage to
the midbrain and the cerebellum. The conditioned reflex to the magnetic field
is almost always fixed later than the conditioned reflexes to light and the bell
in the norm and after removal of the forebrain.

The relative change in the rate of fixation of conditioned reflexes after
damage to separate sections of the brain (Figure 73, B) also coincides with the
graph of the relative change in the rate of appearance of conditioned reflexes,
except that after removal of the forebrain the conditioned reflexes to all the
applied stimuli are fixed sooner than in the norm, and after damage to the visu-
al tegmenta the conditioned reflex to the magnetic field is fixed relatively
sooner than the conditioned reflex to light.

187

a f

—

mil

U/.

?>^

120

(b)

ILM

Figure 73. Absolute (A) and Relative
(B) Changes in the Rate of Fixation of
Electrodefensive Conditioned Reflexes
to a Magnetic Field, Light and Sound
in Fish After Damage to Different Sec-
tions of the Brain. The Designations
Are the Same as in Figure 72. Key:
(a) Number of Applications of the Con-
ditioned Stimulus; (b) Rate of Fixation
of the Conditioned Reflexes, %.

Figure 74. Absolute (A)
Changes in the Stability
of Electrodefensive Con-
ditioned Reflexes to a
Magnetic Field, Light and
Sound in Fish After Damage
to Different Sections of
the Brain. Key: (a) Sta-
bility, %. The Designa-
tions Are the Same as in
Figure 72.

In our opinion, a conditioned
reflex is most completely character-
ized by its stability, and not the
rate of formation of time relation-
ships. But as Figure 7 4, A shows, in
this case the change in stability of
the conditioned reflexes to the ap-
plied stimuli after different types /221
of brain damage supports the conclu-
sions obtained in the analysis of the
formation rate for the time relation-
ships. The conditioned reflex to the
magnetic field was the least stable
reflex. Its stability changed only
after damage to the diencephalon.
The stability of the conditioned re-
flex to light is reduced after any
brain damage. The greatest distur-
bance in the stability of the light
reflex occurred after damage to the
diencephalon. The greatest distur-
bance in the stability of the condi-
tioned reflex to the bell occurred
after removal of the cerebellum, but
after removal of the forebrain the
conditioned reflex to the bell was
not disturbed at all. The relative
change in the stability of the con-
ditioned reflexes shows that the
greatest disturbance occurs in the
conditioned reflex to the bell after
removal of the cerebellum.

To compare the investigated in-
dices of the conditioned reflex we
composed Table 42, which reflects
the relative change in the condi-
tioned reflex after different types
of damage to the fish brain. We
took the value of the index in the
norm as 100%, and the deviations
from the norm are expressed in posi-
tive or negative percents.

From the tables it follows that /222
removal of the forebrain has little
effect on the development of condi-
tioned reflexes. If changes occur,
they are small and not equivalent
for different indices. Thus, if we
judge from the rate of fixation, the
conditioned reflex to the magnet in-

188

TABLE 42. RELATIVE CHANGES IN THE INDICES OF CONDITIONED RE-
FLEXES AFTER DAMAGE TO DIFFERENT SECTIONS OF THE FISH BRAIN.

Index of the con-
ditioned response

Character of the

. disturbance, %

Stimulus

Removal
of the
fore-
brain

Damage
to the
dien-
cephalon

Damage
to the
mid-
brain

Removal
of the
cere-
bellum

magnet

rate of appearance
rate of fixation

-60
+20

- 350
-106

+ 30
+ 80

+ 20
+ 41

stability

+ 2

- 66

+ 8

+ 2

light

rate of appearance
rate of fixation

+58

-275
-124

+ 25
+ 8

stability

-30

- 65

- 52

- 28

bell

rate of appearance
rate of fixation

+ 8

- 33

- 40

-300
- 93

-266
- 40

stability

- 31

- 44

- 75

creased; if we judge from the rate of appearance, it decreased; and if we judge
from the degree of stability of the reflex, it did not change.

Damage to the diencaphalon caused a decrease in the conditioned reflexes to
all stimuli according to all indices. However, the degree of decrease was dif-
ferent for different stimuli.

According to all indices damage to the midbrain increases the conditioned
reflex to the magnetic field a little, reduces the reflex to light a little and
significantly reduces the conditioned reflex to the bell. Upon removal of the
cerebellum, the conditioned reflex to the bell is strongly reduced; the reflex
to light almost does not change in comparison with the norm, and the reflex to
the magnetic field is increased a little.

On the basis of all tests with removal of different sections of the fish
brain we can say that if we quantitatively characterize the fixing function, it
is disturbed to some degree during damage of any section of the fish brain, but
there is a definite localization of this function for different stimuli. Thus,
the conditioned reflexes to light and to the magnetic field are disturbed most
significantly after damage to the diencephalon, and the reflex to the bell, af-
ter damage to the midbrain or cerebellum. These facts coincide with the results
of morphological investigations [Sepp, 1949] according to which the hindbrain is
closely related with the auditory analyser.

Our data do not answer the question about the localization of the fixing
function in fish, since a special investigation is required for this, but we did
clarify the role of different parts of the brain in the development of condition-

189

ed reflexes to a magnetic field in fish. However, if we quantitatively charac-
terize the degree of disturbance of the fixing function after damage to differ-
ent sections of the brain in the development of conditioned rellexes to a magne-
tic field, light and a bell, considering the rate of appearance, the rate of fix-
ation and the stability of the reflexes, then our averages show that the fixing
function is disturbed most after damage to the diencephalon and the midbrain, at
least after damage to the cerebellum. After removal of the forebrain the fix-
ing function is disturbed insignificantly, even during development of complex
conditioned reflexes in fish [Kholodov, I960].

After damage to the diencephalon the development of conditioned reflexes to
a tactile stimulus is also disturbed in fish [Kholodov et al., 1961].

Our data about the possibility of developing conditioned reflexes in fish
after removal of the cerebellum [Kholodov, 1959; Gusel'nikov and Kholodov, 1964]
were recently supported in the work of V. L. Bianki (1962) , in which it was
shown that the formation of time relationships to sound, light and the stimu- /223
lation of a floating bubble in fish is also possible after full removal of this
section of the brain. For a final answer to the question about the localization
of the fixing function in the fish brain we need further investigations with
the parallel application of several methods, including the electrophysiological
method.

Conclusions

1. Denervation of the lateral line organ does not change the conditioned
reflex to a magnetic field in fish.

2. A similarity is observed in the effects of light and a magnetic field;
it is expressed in a generalization of the conditioned reflexes during inter-
change of these stimuli.

3. Bilateral enucleation does not change the conditioned reflex to a mag-
netic field in fish.

4. The similarity in the effects of light and a magnetic field is increased
in blinded fish.

5. The conditioned reflexes to a magnetic field in fish were significantly
disturbed after damage to the diencephalon and did not change after removal of
the forebrain, cerebellum or visual tegmenta.

Synopsis

Using the conditioned reflex method, we managed to elucidate certain proper-
ties of EMF as stimuli, which was also done in tests with the electrographic
method. True, in the rabbit, the only object of the electrographic investiga-
tions, we did not manage to develop a conditioned reflex to a CMF. This fact
can be explained by the late appearance of the basic electrographic reaction to
a CMF in rabbits. Let us recall that the electrical reaction of both the whole

190

brain and separate neurons occurred with a latent period that exceeded 10 sec.
With a 7 sec lag in reinforcement only the desynchronization reaction, which
sometimes starts at the moment the electromagnet is turned on, can occur in the
rabbit EEC. It is evident that this reaction cannot serve as the basis for de-
velopment of a conditioned reflex. Consequently, the experiments on the devel-
opment of conditioned reflexes supported our electrographic data concerning the
long latent period of the reaction of the brain to EMF.

It is fully reasonable to explain the sharp inhibiting effect of EMF during
development of conditioned inhibition in fish and pigeons by the longer (more
than 10 sec) exposure to this factor. More likely, the inhibiting effect of
EMF on previously developed conditioned reflexes is also expressed in the experi-
ments on rabbits, since the inhibiting effect of a CMF on these animals was ob-
served in tests on the reactance curve. Speaking about future experiments, we /224
should also note that recording the brain electrical activity of fish can also
give substantial information on the peculiarities of the reactions of these
animals to a CMF.

At the present time, it is difficult to understand the high sensitivity of
fish to EMF (in comparison with birds and mammals) . We can explain the develop-
ment of conditioned reflexes to a CMF by the peculiarities of the brain struc-
ture (the diencephalon plays the main role in fixing the time relationships)
and the environment (a CMF can act through water). However, a comparison of the
results of tests on developing conditioned reflexes to EMF in fish and the trac-
ings of electrical brain activity in rabbits shows a certain general similarity
of the reactions to EMF in different classes of vertebrates. This includes the
low stability of the reactions, the predominantly inhibiting effect of EMF and
the prolonged aftereffect. While after the experiments with rabbits we spoke
about the similar nonspecific effect of a CMF, a UHF and an SHF field, after
the tests on fish we can speak about the similar nonspecific effect of a CMF,
a variable magnetic field with a frequency of 50 Hz, a UHF field, light and ir-
radiation. Thus, almost the whole spectrum of electromagnetic oscillations can
have a nonspecific effect. on the central nervous system.

In the analysis of the role of receptor formations in the reaction of fish
to EMF, it was observed that denervation of the lateral line organ, enucleation
and damage to the olfactory analyser do not disturb these reactions; and of the
different sections of the brain, the diencephalon plays the most important role.
Let us recall that an isolated brain preparation reacted to EMF better than an
intact brain, and that the hypothalamus was the most reactive structure. Thus,
the predominant interest in the diencephalon appeared during the study of reac-
tions to EMF in both rabbits and fish.

Whereas with the electrographic method there was danger that the effect of
EMF could be transferred by means of the electrodes, so that we had to remove
them during exposure in the control teats, the conditioned reflex method reli-
ably indicates the direct effect of EMF on the organism.

It has already been said that conditioned reflexes to EMF are similar in
their properties to the conditioned reflexes developed from the interoceptros of
the internal organs. This circumstance can be explained by the direct effect of
EMF on the brain tissue, where certain interoceptors are located. However, the

191

conditioned reflexes developed to weak stimulation of the exteroceptors also
have similar properties. It is possible that the peculiarities of the condition-
ed reflexes to EMF can be explained not only by the direct effect of these stim-
uli on the central nervous system, but also by their physiologically weak nature.

192

PART III. OTHER METHODS FOR STUDY- /225
ING THE EFFECT OF ELECTRO-
MAGNETIC FIELDS ON THE
CENTRAL NERVOUS SYSTEM

Although we obtained the basic material on the effect of EMF on the func-
tion of the vertebrate brain by means of electrographic and conditioned-reflex
methods, we also used other methods of investigation which provide additional
information on the nature of the studied phenomenon.

This includes the method of determining the sensitivity to electrical and
chemical stimulation, the method of recording motor activity, and histological
investigations of the changes in the brains of animals subjected to EMF.

In contrast to the two preceding parts, in this part of the book we expound
the results of experiments in which the effect of EMF was not limited to seconds,
but lasted for minutes, hours and even days. This circumstance allowed us to
reveal the more intensive EMF effects.

At the same time, we were limited to just a description of the obtained
phenomena, without offering the physiological analysis which was used in the
preceding chapters.

193

CHAPTER 7. THE CHANGE IN THE SENSITIVITY OF FISH A^D /22&*

AMPHIBIANS TO A MAGNETIC FIELD OR LIGHT

We assumed that fish perceive a magnetic field, like light, directly by the
diencephalon . It is known that chemical or electrical stimulation of the dien-
cephalon leads to inhibition of the signaling reflexes in frog [Sechenov, 1863] .
We decided to check whether a similar effect occurs during the influence of
light or a magnetic field directly on the diencephalon.

Investigation of Sechenov Inhibition During the Influence

of Light or a^ Magnetic Field on the Diencephalon

of Frogs with Their Hemispheres Removed

The tests were conducted according to the following method. The upper jaw
together with the eyes was cut away. The cerebral hemispheres were removed.
The skin was removed from the remaining upper part of the skull. The trunk of
the frog was fastened horizontally on a test plate and its hind legs were held
in the vertical position. The entire frog was covered with a black opaque paper
in which a hole was cut for illumination of the diencephalon. Either the light
from a 25-w electric light passed through a water filter 6-8 cm thick, or a mag-
netic field with a frequency of 50 Hz and a strength of 500-800 Oe was employed
on the region of the diencephalon. The magnetic field was created by an elec-
tromagnet with its core placed over the frog diencephalon.

The tests were conducted in a darkened room. The feet of the frog were
stimulated with a 1.0-1.5% sulfuric acid solution. The glasses containing the
acid were filled such that all the toes of the frog were immersed in the liquid.
After the frog removed its foot from the acid, the stimulated foot was washed
with water and the other foot was immersed in the acid after 1-2 min. The la-
tent period of the signaling reflexes was measured with an accuracy up to 1 sec.
A foot was stimulated only once.

The effect of light on the diencephalon was studied in 19 frogs. The gen-
eral results of the tests are given in Table 43.

The data of Table 43 show that an increase in the time of the signaling re-
flex, i.e., Sechenov inhibition, is observed most frequently (in 77% of the
cases) during the influence of light on the frog diencephalon. Inhibition of
signaling reflexes has also been noted by other authors during total- body illu-
mination of a frog [Johannes, 1930; Beburishvili, 1937]. /227

Figure 75 gives the results of the most typical test. We see that when
there was no influence on the diencephalon, the latent period of the signaling
reflex changed little over 5 min, being approximately equal to 10 sec. But, 1
min after the beginning of light exposure on the diencephalon, the latent period
of the reflex increased by a factor of 3. Inhibition was complete in 4 min.
The inhibiting effect caused by light is also retained after this stimulus is
turned off. Only 10 min after the light is turned off does the length of the

194

TABLE 43. CHANGE IN THE LATENT PERIOD OF SIGNALING REFLEXES
DURING THE INFLUENCE OF LIGHT ON THE FROG DIENCEPHALON .

60
O
U

CO
4J
CO
(U
4J

Character of the
change (number of

latent period
cases)

•

CO
4J
CO

Character of the latent period
change (number of cases)

absence

absence of

=5!=

xncrease

change

decrease

=«=

increase

change

decrease

3
2

4
2

3
2

Total:

raw

100

latent perxod begin to return to the initial level. Since the observed effect
has a reversible character, the results of this test indicate the presence of a
light effect directly on the diencephalon. The effect of light appears after a
defmxte latent period and is retained for a significant time after the light is
turned off.

The change in the signaling reflexes was observed from 1 to 21 min after the
start of the light exposure, but only in rare cases did it begin after 6 min.
in 9n^™^ °^ ^^^ aftereffect varied from 3 to 57 min, but in most tests it was
10-20 min. The aftereffect was always longer than the latent period.

Table 43 shows 5 cases in which the influence of light directly on the di- /229
encephalon did not increase the time of signaling reflexes. However, this ef-
T«7Qi*^^ ^°^^^ during the exposure to light on the skin of the frog [Wwedensky,
1879]. A detailed analysis of these tests showed that in all 5 cases the light
acted on an inhibiting background. Figure 76 shows that under the influence of
light there is inhibition of the signaling reflexes during stimulation of the
right foot with acid and disinhibition during stimulation of the left foot in
the same frog. Inhibition passes soon after the light is turned off, but the
disinhibition is retained throughout the tests. Consequently, the effect of
light depends on the initial functional state of the nervous system, and the in-
stances of a decrease in the latent period of signaling reflexes must be related
to tests that also support the perception of light during its direct effect on
the diencephalon. Then, according to the data of Table 43, the number of tests

195

5U
W
30
20
10

(a)

JjJL

f 3 5 7

\ —

(b)

3 ft 13 15 17 19 21 23 25 MUH

Figure 75. Increase in the Latent Periods
of Signaling Reflexes During the Direct In-
fluence of Light on the Frog Diencephalon.
The Arrows Note the Beginning and the End
of the Exposure; the Light Columns Desig-
nate the Absence of a Reaction over 1 Min;
the Time of the Test (in Min) is Displayed
Along the Abscissa, and the Time of the
Latent Period (in Sec), Along the Ordinate.
Key: (a) Sec; (b) Min.

13 n 21 ZS 23 33 37 VI ¥5 mum.

S \9 13 n 21 25 29 33 37 VI VS muh.
* ^^—^

Figure 76. Changes in the Latent Periods
of Signaling Reflexes in Two Feet of a Frog
During the Influence of Light on the Dien-
cephalon. A = During Acid Stimulation of
the Left Foot; B = the Right Foot. The Re-
maining Designations are the same as in
Figure 75. Key: (a) Sec; (b) Min.

that indicate the presence of a
light effect will be 77 + / =
84%. In a detailed analysis of
the cases involving an absence
of a light effect, we revealed
two factors: first, it was ob-
served most frequently in males;
second, the absence of a light
effect was observed with a la-
tent period for the signaling
reflex of less than 5 sec, i.e.,
in cases when the acid was a
very strong stimulus.

The effect of a variable
magnetic field with a strength
of 500 Oe was studied in 37
tests on 9 frogs (Table 44) .

From Table 44, it follows
that the latent period of sig-
naling reflexes during the in-
fluence of a magnetic field in-
creased in 58% of the cases, de-
creased in 13% and remained un-
changed in 19%.

Figure 77 shows the results /230
of one of these tests. It is
evident that the influence of a
magnetic field causes inhibition
of the signaling reflexes in the
frog. An analysis of the length
of the latent period and the
time of aftereffect showed that
the effect of a magnetic field
occurs, on the average, 8 min
after the electromagnet is turn-
ed on, and the aftereffect lasts
approximately 5 min after it is
turned off. Consequently, if
we judge from the length of the
latent period and the after-
effect, a magnetic field is a
weaker stimulus than light.

An analysis of the cases
in which the latent period of
the signaling reflexes was re-
duced showed that, like light,
a magnet changes the signaling

196

Cex (a)

140

30
20

lULI

(b)

/ 3 k5 7 9 11 13 IS n 13 Z1 muh.

Figure 77. Increase In the Latent Peri-
ods of Signaling Reflexes During the In-
fluence of a Magnetic Field on the Frog
Brain. The Designations are the same as
in Figure 75. Key: (a) Sec; (b) Min.

reflexes in a way that depends on
the initial state. As the test re-
sults given in Figure 78 indicate,
a magnetic field decreases the la-
tent period of the signaling re-
flexes on an inhibiting background.
In some cases this decrease is ir-
reversible, while in other cases
it is reversible in nature, i.e.,
the latent period again increases
soon after the end of the exposure
to the magnetic field.

As in the case of an increase
in the latent period, the cases of
a decrease in the latent period of
signaling reflexes also indicate
the presence of a magnetic field

TABLE 44. CHANGES IN THE LATENT PERIOD OF
SIGNALING REFLEXES DURING THE INFLUENCE OF
A MAGNETIC FIELD ON THE HEAD OF A FROG.

60
O
U

O (0

iH 0)
Cd 4J

■u

O <+-!

H O

Character of the latent period
change (number of cases)

increase

absence of
change

decrease

Total:

raw

100

effect. In total, we find that the effect of the magnetic field occurs with /231
68 + 13 = 81% of the exposures to this stimulus, i.e., a little less often than
during the influence of light (84%) .

197

We conducted tests on the effect of a magnetic field on frogs after removal
of the diencephalon, but did not observe changes in the latent period of re-
flexes. This is ill agreement with the data of other authors [Drozdov, 1879].

Oeif (a)

30
ZO
10

,1 ■■■, ■ ■ ■

,(b)

Q J 3 5 1 9 If 13 15 n 19 It MUH

Figure 78. Reversible (A) and Irre-
versible (B) Decreases in the Latent
Period of Signaling Reflexes During
the Influence of a Magnetic Field on
the Frog Brain. The Remaining Desig-
nations are the Same as in Figure 75.
Key: (a) Sec; (b) Min.

Although the electromagnet was
placed above the diencephalon in all
our tests, we could not strictly lo-
calize the magnetic field exposure,
which was propagated to the surround-
ing tissue to some degree. However,
the influence of the electromagnet on
a section of the spinal cord did not
cause Sechenov inhibition. Based on
this, we concluded that the magnetic
field acts directly on the diencepha-
lon.

The direct effect of a magnetic
field on the nervous system was noted
at the very start of the experimental
study of the physiological properties
of this stimulus. More recent works
contain indications of the possibility
of a direct effect of a magnetic field
on the brain [Sherstneva, 1951; /232
Selivanova and Erdman, 1956] . Our
data (which agree with the references)
offer a somewhat more specific locali-
zation of brain sections most sensi-
tive to a magnetic field. These should
probably include the hypothalamus re-
gion of the diencephalon. It is in-
teresting to note that the hypothala-
mus is also the site for perception of
light [Lisk and Kannwischer, 1964; and others], tem-

other physical factors. — „-_ ,

perature [Euler, 1950; and others], ionizing radiation [Aladzhalova , 1962; and
others] and a UHF field [Livshits, 1958; and others] during their direct effect
on the brain. Thus, the question regarding the mechanism of the perception of
a magnetic field has become part of the large problem concerning the receptor
function of the hypothalamus.

The direct effect of stimuli on the central nervous system show that the
reflex principle of nervous system activity should be more widely understood
than the classical diagram of the reflex arc allows. The afferent part of the
arc of certain reflexes can be reduced to extremely small dimensions, while the
receptor part actually merges with the central juncture. In principle, the
possibility of this diagram has been shown by numerous tests using electrical,
mechanical and chemical stimulation of the central nervous system, and by tests
on development of conditioned reflexes to electrical stimulation of the brain
[Rozhanskiy, 1957; and others].

198

The efferent part of the reflex arc can also be absent during the influence

an TfTT 'r™"''- .^' '• ^"P"'°" ^'''^> '^"-^-^ conditioned refLxes Without
an effector termination, class I conditioned reflexes, and reactions durinrSl
rect stimulation of the central nervous system, class 'll conditioned reflexes

The impossibility of developing conditioned reflexes to a magnetic field in
pigeons shows that the effector part of the reflex arc is absent during the in-
fluence of a magnetic field. We found that the magnetic stimulus invofves
neither an afferent, nor an efferent part of the reflex arc i e thP IfL.^ ^
the magnetic field is limited to the central nervous sysLm! E^;; within the

ixcit^tJfn %''"'"' r^^^"^ ^^^'^'"' ^ '"^^^^^^^ field^does not Sule spreading
excitation. For example, in fish the conditioned reflex to a magnetic field if

daml^e to otW b''°"'"" T"^' '° '^^ diencephalon, and does no? ch^ge during
damage to other brain sections. In a neuromuscular preparation, a magnetic

Erdian?"l9?6]? ^^^' ^""^^ ^° "°' '""""'' ""' ""'^"'^"" parabiosis [PetrSv. 1930;

The Effect of Light or a Magnetic Field 7233

on the Sensitivity of Fish and

Axolotl to an Electric Current

The change in the sensitivity of fish to an electric current during different
llllTT. r'-"°'' f-q-^ntly determined by the change in the anesthetization
tnreshold during exposure to a variable electric current [Puchkov 1954-

tlon^orfii^^^i' ^'^" ^''°'t ""^^ ''^^''^^ ^" ^^^ ^°ltage that causes immobiliza-
thT ... /°r''^^' ^"^h a strong stimulus causes prolonged changes in
r^nrll^^^.° ne^ous system. We are supposing that the threshold of cur-

c.^rr.nr^''^^ '^ u^"" ^^"^^ ^^ '^^ ^^^^^ °^ sensitivity of fish to an electric
elect.?. ""^^ ^?^ ""^"^ procedure of determining the sensitivity of fish to an
inH^r ^-^u^'r t°^^ ''°^ ""^^""S^ ^^^ current perception threshold. This latter
r^J!^^ . ff.^^^" determined for decades, remains at the same level
IKholodov and Akhmedov, 1962].

The ^rn-npr?^^^?^"""" "f Conducted on different species of fish and on axolotl.
n^r/n ^ diagram of the device is shown in Figure 79. Lead electrodes were
placed on two opposite walls of the aquarium. A direct current from battery
with^'the ^Jn^'of'^^r ^l^f^°^es through a rheostat (P) . The circuit was switched
with the aid of a key (K) and the applied voltage was measured by means of a
voltmeter (B) . Before determining the perception threshold of an electric cur-
rent, we always placed the test animal in the center of the aquarium and directed
J^ P^^P^°^^^"^^^ to the plane of the electrodes with its head toward the cathode.
! u ^ increasing the voltage to the electrodes, we found the voltage that
caused the primary tremor reaction in the fish. This determination was conducted
J times m a row and, if the readings were close, the average of these measure-
ments characterized the sensitivity of the fish to an electric current at the
given moment.

Then the fish was subjected to the influence of the studied physical factor
light or a 50-200-Oe magnetic field. Illumination was accomplished with a 50-w
electric light, and the magnetic field was created by switching on the solenoid
wound around the aquarium.

199

Figure 79. Diagram of the
Device for Determining the
Sensitivity of Fish to an
Electric Current. See Text
for a Description.

During the Influence of the studied factor, ^^^ _
we determined the threshold of per cep Lion of an / ^-''*
electric current by the fish according to the
method described above. However, this determi-
nation of the perception threshold was conducted ■
some time after the studied factor was switched
off. We counted the readings of only the tests
during which the sensitivity of fish to an elec-
tric current before and after exposure to the
studied factor was approximately identical.

The tests were conducted on stickleback
(the White Sea Biological Station of Moscow State
University), bleak, quab, brook trout, Raja
clavata, striped perch, bullhead, roach, Proxi-
mus , rudd, Leucaspius and carp (Zvenigorodsk
Biological Station of Moscow State University) .

The effect of the CMF on the sensitivity of
marine fish to an electric current was investi-
gated on 20 stickleback. The test results are shown in Figure 80, which gives
the average perception thresholds for each 10 fish. We see that the magnetic
field increases the threshold of perception of an electric current by 17-21/. on
the average. The fact that an increase in the perception threshold is observed
only during the influence of the magnetic field. Indicates the reliabxlity of
the obtained results. The test results on freshwater fish are given in Table
45.

From Table 45, it is evident that
the effect of a magnetic field on the
sensitivity of fish to an electric cur-
rent is observed in all 9 species of fish.
This effect is most frequently (65% of
the cases) manifested as a decrease in
the sensitivity of fish to an electric
current, but there were tests in which
the sensitivity was Increased during the
Influence of the magnetic field. These
include the tests on striped perch, bleak
and Proximus . These fish were less able
to withstand the conditions of the sole-
noid in the aquarium than the others, and
they died soon after the tests. A high
threshold of perception of an electric
current was characteristic for them. To
illustrate this, we offer the records of
two tests on the same Proximus .

0/ m2 ^3

Figure 80. Average Sensitivity
of Stickleback to an Electric
Field Before (1) during (2) and
after (3) exposure to a CMF. A =
test No. 1 (stickleback No. 1-10);
B = test No. 2 (stickleback No.
11-12).

creases it (Test No. 2), but the same

In the case of a low initial percep-
tion threshold, the magnetic field in-
magnetic field, acting on the background

200

TABLE 45. CHANGE IN THE SENSITIVITY OF FRESHWATER
FISH TO AN ELECTRIC CURRENT DURING EXPOSURE TO A
MAGNETIC FIELD.

/235

Change in

Species of

Number of

sensitivity

Absence of

Fish

tests

the effect

decrease

increase

Ra.ia clavata

quab

striped perch

bleak

bullhead

roach

brook trout

Proximus

rudd

Total:

raw

100

RECORDS OF TESTS ON PROXIMUS NO. 2.

Test no. 2

Test no. 3

time

perception
threshold

time

perception
threshold

before exposure
during exposure
after exposure

16 hr, 46 mln
16 hr, 51 mln
16 hr, 53 min

16 hr, 58 min

17 hr, 02 min
17 hr, 04 min

17 hr, 10 min
17 hr, 13 min
17 hr, 16 min

1.4
1.4
1.4

1.7
1.7
1.8

1.2
1.4
1.4

12 hr, 09 min
12 hr, 11 min
12 hr, 12 min

12 hr, 16 min
12 hr, 19 min
12 hr, 21 min

12 hr, 31 min
12 hr, 35 min
12 hr, 39 min

3.6
3.7
3.7

3.3
3.4
3.0

3.8
3.6
3.6

of lowered sensitivity of the fish to an electric current, causes a lowering of
the perception threshold. Consequently, the result of an exposure to a magnetic

201

field is determined by the initial functional state of the nervous system of the

fish and, to quantitatively characterize the effect of a magnetic field on the

senslLivity of fish to an electric current, we must sum all the cases of a change

in the sensitivity. Thus, the effect of a magnetic field is observed in 65 +
22 = 87% of the treated cases.

The general results of this series of tests are similar to the results of /236
tests on frogs. A change in the sensitivity of the animal to an external stimu-
lus during the influence of a magnetic field is observed in both cases, but dur-
ing both electrical and chemical stimulation, a magnetic field most frequently
reduces the sensitivity. However, depending on the initial functional state,
the magnetic field can also increase the sensitivity. Consequently, a magnetic
field somehow regulates the sensitivity of an organism to different influences.
This general regulation can be accomplished only through the central nervous
system, and we can assume that the change in the function of the CNS is a re-
sult of the influence of the magnetic field.

We also checked the hypothesis regarding the similar effect of light and a
magnetic field during the investigation of the sensitivity of fish to an elec-
tric current. The method of investigation in determining the effect of light on
the sensitivity of fish to an electric current was the same as during the in-
vestigation of the effect of a magnetic field. The test objects were the same
freshwater fish and also axolotl. The general test results are given in Table
46.

TABLE 46. CHANGE IN THE SENSITIVITY OF FISH TO AN
ELECTRIC CUREIENT DURING THE INFLUENCE OF LIGHT.

Changes in

Species of

Number of

sensitivity

Absence of
the effect

Fish

tests

decrease

increase

bleak

quab

brook trout

Raia clavata

bullhead

roach

Proximus

rudd

Leucaspius

carp

Total:

raw

100

202

The data of Table 46 show that most frequently (In 71% of the cases) light
increases the sensitivity of fish to an electric current, rarely reduces it (23%)
and only very rarely does not change the sensitivity (6%). The cause of the de-
crease in the sensitivity to an electric current observed in rudd and quab dur- /237
ing the influence of light is still unclear. However, regardless of these cases,
the general conclusion concerning the increase in the sensitivity of fish to an
electric current during illumination remains in force.

Consequently, light and a magnetic field affect the sensitivity of fish to
an electric current differently. This conclusion casts doubt on our assumption
regarding the similar effect of a magnetic field and light on fish. However,
in tests on development of conditioned reflexes, we established that the simi-
larity in the effect of a light and a magnetic field is increased after the fish
are blinded. Therefore, we decided to check how the sensitivity to an electric
current changes during the illumination of 7 blinded fish.

All the tests gave an identical result: in blinded fish the sensitivity
to an electric current was lowered during the influence of light. This shows
that, first, light receptors remain in fish after blinding and, second, the
photoreceptors of the blinded fish possibly are qualitatively different from
the retina, which reduces the sensitivity of fish to an electric current.

The tests involving blinded fish showed that the similarity in the effect
of light and a magnetic field is revealed only after removal of the specific
light receptor, the retina. The operation of blinding, by itself, either gener-
ally did not change the sensitivity of fish to an electric current, or changed
it in one or the other direction, but illumination always reduced the sensitiv-
ity to an electric current.

Figure 81 shows the results of tests conducted on the same day and on the
same fish before and after blinding. Before blinding, the sensitivity to an
electric current increased during the influence of light, and after blinding, it/238
was reduced by an average of 1.0 v.

5.0

I 10

^ 2.0

(a)
>,o

15950 5Z 5* SS Se mvOZ OV 21'3 /5 n ts zi 23 25 21
Pq\ Bpe^n uccjiedotaMuii

Figure 81. Change in the Threshold of Per-
ception of an Electric Current in a Brook
Trout During the Influence of Light Before
(A) and After (B) Blinding. The Horizontal
Line Designates the Time of the Light Influ-
ence. Key: (a) Threshold (b) Time of Test.

We obtained similar results
not only in other species of
fish, but also in axolotl. The
sensitivity to an electric cur-
rent in axolotl did not change
after blinding. In both blinded
and normal axolotl, the threshold
of perception of an electric cur-
rent was approximately 1 v. How-
ever, the change in the sensitiv-
ity during the influence of light
in blinded and normal axolotl has
a different direction, although
approximately an identical mag-
nitude. Consequently, following
blinding in both axolotl and
fish, light continues to have

203

an effect on the physiological state of the CNS.

Discussion

Tests on the change in the sensitivity of fish to an electric current dur-
ing the influence of a magnetic field or light again showed that a magnetic
field has a physiological effect, that the character of this effect is similar
to the character of the effect of light on blinded fish, and that its result is
a change in the sensitivity to chemical and electrical stimuli. The different
effects of light on blinded and normal animals force us to assume a dual nature
of the influence of light on the organism. One mechanism of the influence,
through the retina, basically ensures the function of objective vision; the
other mechanism of the influence, which is effected through both the retina and
other paths, affects many functions of the organism [Godnev, 1882; Svetozarov
and Shtraykh, 1941; Markelov, 1948; Berkovich, 1953].

The obtained results show that a magnetic field can act not only on birds
and fish, but also on amphibians. Generalizing our data and the reference data,
we can speak of the possibilities of an effect of a magnetic field on all
classes of vertebrates. While the sensitivity to a magnetic field plays some
role in the long-range migrations of fish and birds, the development of this
sensitivity occurred on the basis of a general (for all vertebrates) ability to
perceive a magnetic field.

Conclusions

1. During the direct effect of light or a magnetic field on the diencepha-
lon of frogs without hemispheres, changes are observed in the length of the la-
tent period of signaling reflexes.

2. The direction of the change in the latent period of signaling reflexes
under the stated influences depends on the initial functional state of the
nervous system. On an inhibited background, the signaling reflexes are accel-
erated, but on an excited background, they are inhibited; the last case is ob-
served more frequently.

3. Judging from the latent period and the time of the aftereffect, light /239
affects the diencephalon more strongly than a magnetic field.

4. A magnetic field reduces the sensitivity to a constant electric cur-
rent in marine and freshwater fish.

5. In normal fish and axolotl, light Increases the sensitivity to a con-
stant electric current, but in blinded animals, it reduces it.

6. The similarity in the effect of light and a magnetic field is revealed
in the change of sensitivity in blinded fish.

204

CHAPTER 8. THE CHANGE IN THE MOTOR ACTIVITY OF FISH AND BIRDS
DURING THE INFLUENCE OF A CONSTANT MAGNETIC FIELD

In studying the effect of a magnetic field on fish by the development of
conditioned reflexes to this factor, and also by determining the sensitivity to
an electric current, we tried to investigate the activity of the whole organism.
It seemed to us that to some degree this approach helped reveal the physiologi-
cal effect of such a weak stimulus as a magnetic field. We also tried to find
other methods of studying the activity of a whole organism in order to once
again check the possibility of the physiological effect of a magnetic field on
animals. One such method is that of determining the motor activity. These
tests were conducted on fish and birds.

The Change in the Motor Activity of Stickleback
During the Influence of a_ CMF

We recorded the motor activity of the fish by a very simple method. The
stickleback were placed in a tank around which a solenoid was wound; this sole-
noid, connected to a dc circuit, created a 50-150-Oe magnetic field. One end
of a thread was tied to the dorsal fin of the fish, the other end was fastened
to a lever whose motion was recorded on the smoked drum of a kymograph. This
recording did not allow us to quantitatively characterize the motions that were
observed during the influence of the magnetic field, but it demonstrated the
presence of this effect with sufficient persuasiveness (Figure 82) . On the ky-
mogram, it is evident that during a 1-hour exposure to a magnetic field, the /240
motor activity of the fish was greater than before or after exposure. The in-
fluence of the magnetic field was not felt immediately after turn-on, but after
15 min had passed. The effect was also retained for some time after the magnet-
ic field was turned off. However, we could not give a persuasive quantitative
characterization of the effect of a magnetic field on the motor activity of a
fish due to the imperfection of the method used. Therefore, we have summarized
the test results in the form of Table 47, which shows how frequently the effect
of the magnetic field was observed.

v44itTHifMf-^4^f^^

T r

T T

Figure 82. Increase in the Motor Activity
of a Fish During Exposure to a CMF. M =
Influence Period of the Magnetic Field.
The Time Markings Equal 2 Hours.

Most frequently (64% of the
cases) a magnetic field increase d/ 241
the motor activity of stickleback.
A decrease in motor activity dur-
ing the influence of the magnetic
field was rarely observed (in 15%
of the cases) . We observed the
absence of an effect in 23% of the
cases exposed to the magnetic
field.

Consequently, a magnetic
field increases the motor activity
in fish and, in this, its effect

205

TABLE 47. THE EFFECT OF A MAGNETIC FIELD
ON THE MOTOR ACTIVITY OF STICKLEBACK.

•

•H

Number of
tests

Character of the change in
motor activity

Absence of
the effect

increase

decrease

2
3
4
5
6
7
8
9
10
11

2
1

22
1
1
5

13
8

1
2
6

2
8
3

Total:

raw

85
100

54
64

11
13

20
23

is similar to the known effect of light on the motor activity of fish [Jones,
1955; Voronin and Kholodov, 1962; and others]. The similar effect of a magnet-
ic field and light in the change in motor activity forces us to assume that the
effect of light on motor activity is realized not only by means of the retina,
but by another means as well. Tests on blinded fish prove the assumption of
Woodhead (1958).

The Change in the Motor Activity of Birds
During the Influence of a CMF

The method of recording motor activity under laboratory conditions is ap-
plied most frequently in the study of the behavior of birds. Numerous methods
of recording motor activity have been developed for this class of vertebrates.
This index is frequently used by ornithologists and physiologists in studying
the effect of external and internal factors on the behavior of birds. The meth-
od of determining the motor activity of birds allows us to quantitatively char-
acterize the level of the organism's activity. Therefore, we decided to con-
duct experiments on the effect of a magnetic field on the motor activity of
birds from the sparrow family (4 bullfinches, 2 greenfinches, 2 titmice, 1
crossbill and 1 chaffinch). This work was conducted together with A. L.
El'darov.

The bird was placed in a wooden cage (Figure 83) , the floor of which was
suspended on rubber bands. The perches were fastened to the floor, and.

206

therefore, any movement of the bird from perch to perch forced the floor to os-
cillate. By dropping down, the floor closed electrical contact (K) , which was
switched into the circuit of the battery (A) and the electromagnetic marker (0).
Deflection of the electromagnetic marker lever ensured rotation of the anchor
wheel (An) by one tooth. The anchor wheel was set on the axis of the van wheel
of the kymograph (Kia^) such that 24 closures of the marker rotated the kymograph

drum by 1 mm. The glass ink stylus was connected by means of blocks with the
drum of the second kymograph (Km2) and moved vertically at a speed of 1 cm/hr.

Consequently, on the tape of the first kymograph we obtained a curve on whose
horizontal the total number of motions of the bird during one test was recorded,
and along the vertical, the time of the test. The test lasted 2 or 9 hours
(from 0900 to 1800 hr) .

Figure 83. Diagram of the Device for Recording the
Motor Activity of Birds (Description in the Text).

A constant 0.70-1.70-Oe magnetic field was created by switching Helmholtz /242
colls wound around the cage into the circuit of the battery. We began working
with each bird in such a way that we could record its motor activity without the
influence of a CMF by means of several tests. Thpn, one test was completely con-
ducted with the Helmholtz coils turned on, and after this we again recorded the
motor activity without a CMF for several days.

At the beginning of this work, we recorded the diurnal variations of the
air temperature and humidity, and also atmospheric pressure. However, signifi-
cant changes in the motor activity of birds were noted during stable environ-
mental conditions, and they basically depended on the degree of illumination.
The Increase in bird motor activity during the Influence of light has been noted
by many investigators and, therefore, the increase in motor activity of bull-
finches which we observed during the Influence of a magnetic field during natu-
ral illumination (the cage stood on a windowslll) could be explained by the in-
fluence of Illumination, the magnitude of which we did not measure. For a
stricter clarification of the effect of a magnetic field, we conducted the basic

207

tests with artificial illumination. The artificial illumination was from a 50-w
electric light placed over the glass ceiling of the cage. In another case, we
created weak artificial illumination in the cage. We judged the effect of a CMF
by comparing the number of bird movements during the test involving the influ-
ence of the CMF with the average number of movements during the test without
this stimulus. The results of all tests are given in Table 48.

TABLE 48. INFLUENCE OF A MAGNETIC FIELD ON BIRD MOTOR ACTIVITY.

Species of bird

bullfinch no. 1
bullfinch no. 2
bullfinch no. 3
bullfinch no. 4
greenfinch no. 1

total:

raw

greenfinch no. 2
titmouse no. 1
titmouse no. 2
chaffinch no. 1
crossbill no. 1

total:

raw

total of both
series:
raw
%

Duration

of the CMF

exposure, hr.

9
9
9
9
9

2
2
2
2
2

Number
of tests

12
2
9
4
1

28
100

1
7
5
1
6

20
100

48
100

Character of the
change in the
motor activity

increase

12
2
6

25
90

1
5
5
1
5

17
85

42
88

decrease

2
7

Absence of
the effect

3
15

5
10

1
3

1
2

Most frequently (88% of the cases) the magnetic field, acting for 2 or 9
hours, increases motor activity. Tests in which there was a decrease in motor
activity during the influence of the magnetic field, and in the absence of this
effect, compose 12% of all exposures to the magnetic field and, what is inter- /243
esting, all these involved females. It is possible that males are more sensi-
tive to a magnetic field.

Comparing the results of these tests with similar tests on stickleback
(see Table 47) , we can conclude that the motor activity of birds during the

208

influence of a magnetic field changes more frequently than the motor activity of
fish, although this comparison is very relative. However, although we cannot
compare the results of the tests on stickleback and bullfinches from a quantita-
tive point of view, we can qualitatively characterize them unambiguously, i.e.,
a magnetic field increases the motor activity of fish and birds.

Since the motor activity of birds strongly depends on illumination
[Kalabukhov, 1951; Eyster, 1954; Segal', 1955], we conducted tests on the effec t/244
of a magnetic field during illumination of different intensities. Figure 84
shows the results of tests with bullfinch no. 1, on which we conducted experi-
ments using natural (variable) illumination and constant artificial strong and
weak illumination. The test results show that under any illuminating conditions,
a CMF increases the motor activity of the bullfinch. The degree of this in-
crease, however, depends on the initial amount of the motor activity, which is
determined by both the intensity of illumination and by certain unconsidered
factors, because the amount of motor activity varied rather widely under identi-
cal illimiination.

22000

A A

20000

\ /\

moo

\/ \

/ \

tBOOO

1 f/OOO
% 12000

■\

K V

%, 10000

■ \

/ \/

^ 8000

% SOOO

(a) tOOO

2000

■I

_i 1 1 — 1 1 — 1-

1 2 3 9 5 6 7 8 3 10 11 12
(b) ffoMcp npaMBHeHUH MasMuma

Figure 84. Motor Activity of Bullfinch No. 1
Before (1) and During (2) Exposure to a CMF
Under Conditions of Natural (A) , Constant
Strong (B) and Weak (C) Illumination. Key:
(a) Number of Movements; (b) Magnet Application
Number.

ly under weak illumination (4.3 times).

Under natural illumina-
tion, the average amount of
motor activity during a test
was 3,000 movements, under
strong artificial illumina-
tion, 5,400, and under weak
artificial illumination,
2,600. During the influence
of the CMF, we recorded
7,500, 17,900 and 11,150
movements per test. From
this it follows that the back-
ground motor activity is low-
er under weak, and higher un-
der strong constant illumina-
tion, and during the influence
of the magnetic field, the
motor activity increases less
under natural illumination
and more under artificial il-
lumination, i.e., the effect
of the magnetic field is re-
vealed better at some con-
stant level of illumination,
and the relative increase in
the motor activity during /245
the influence of the magnetic
field is revealed most clear-

Having established that a general increase occurs in the motor activity of
bullfinches during the influence of a CMF, we decided to analyze the qualitative
peculiarities of the increase by studying the dynamics of motor activity through-
out the test. To do this, we constructed a graph of motor activity plotting

209

time, expressed In hours, on the abscissa and the number of bird movements per
hour on the ordinate. The graphs were constructed from the average values for
all birds at the given illuminatiou. One line designates the average values for
all our background tests, and the other, the average values for all tests in-
volving exposure to the CMF.

In Figure 85, we see that the motor
activity during artificial illumination has
2 peaks during the test, one at 1000 and the
other at 1700 hours. Only the first peak,
which coincided in time with the morning
peak of the background tests, appeared during
the influence of the CMF. During strong
artificial illumination, the graph of bull-
finch motor activity retained the peaks ob-
served under natural illumination, but these
peaks were flattened out and the morning
peak was shifted from 1000 to 1100 hours.
On this background, the influence of the CMF
sharply changes the dynamics of motor activ-
ity. In this case, the motor activity in-
creases from the morning, by 1400 hours it
attains its maximim, and after this it slow-
ly begins to drop. Under conditions of weak
illumination the motor activity of the bull-
finch continued only to 1500 hours, attained
its maximum at 1200 hours. The CMF in-
creased the duration of the motor activity
to 1800 hours and shifted the activity maxi-
mum to 1300 hours.

The test results show that during con -/ 246
ditions of constant artificial illumination,
a CMF changes the dynamics of the motor ac-
tivity during the test, causing a single-
peaked curve with a maximum at 1300-1400
hours. During conditions of natural illumi-
nation, however, the effect of a CMF is man-
ifested primarily as an increase in motor
activity, retaining the same type of dynam-
ics throughout %he test. Consequently, both
the quantitative and the qualitative changes
in motor activity during the influence of a
magnetic field are revealed more clearly
during conditions of constant illumination.

fff iZ IH IB 18
(b) BpcMR C(/moK, vacti

Figure 85. Dynamics of Motor
Activity Throughout the Test in
Bullfinch No. 1 Under Conditions
of Natural (A) or Artificial
Weak (B) and Strong (C) Illumina-
tion Before (1) and During (2)
Exposure to a CMF. Key: (a)
Number of Movements; (b) Time of
Day, Hours.

From the results reflected in the
graphs, we can conclude that a magnetic
field increases the motor activity of birds even during the first hour of its
influence. We see that at 1000 hours, the motor activity is higher during ex-
posure to the magnetic field than in the control tests. A detailed analysis of
all tests on all birds shows that the latent period of the effect of the magnetic

210

field IS less than 6 min in any case. Our methodology did not allow a more ac-
curate determination of the duration of the latent period. The results of tests
involving a 2-hour exposure to a CMF showed that in 75% of the cases the in-
creased motor activity of birds was retained for 2 hours after the exposure.

Under conditions of artificial illumination, the bullfinch usually stopped
moving ijnmediately after the light was turned off. In tests involving exposure
to a CMF, however, switching off the light did not affect the motor activity of
birds (Figure 86), which stopped only several minutes after the solenoid was
turned off. This fact clearly demonstrates the presence of a magnetic field
effect on the motor activity of bullfinches.

Figure 86. Retention of Motor
Activity of Bullfinch No. 1 Dur-
ing Exposure to a CMF After the
Light is Turned Off. A = Bull-
finch No. 1, Test No. 27, January
31, 1957 (the Arrow Shows When the
Light was Turned Off); B = Bull-
finch No. 1, Test No. 28, February
1, 1957 (the Arrow to the Left
Shows When the Light was Turned
Off; the One to the Right, When
the Magnet was Turned Off).

Discussion

In tests involving the application of
other methods, we most frequently observed
the inhibiting effect of a magnetic field,
while in recording the motor activity its
predominantly stimulating effect was re-
vealed. While in the tests with birds /247
we can assume that a very weak field
causes excitation and a stronger field
causes inhibition, in the tests with
stickleback the same field intensity
caused inhibition of conditioned reflexes
and an increase in motor activity. We
cannot now fully explain the cause of
these contradictory results, but we will
give some commentary on this conclusion.

The increase in motor activity during
exposure to a CMF and recorded in tests on
11 fish and 12 birds, again shows that a
CMF acts as a correcting stimulus. In ex-
ternal appearance, the increase in motor
activity during the influence of a magnet-
ic field is similar to the increase in the
number of intersignal reactions during de-
velopment of a positive conditioned reflex
to a magnetic field in goldfish and pig-
eons. If we consider that motor activity
includes reactions to conditioned stimuli,
a similarity in these indices with respect to
. this can explain only part of the increase in
motor activity, which includes the unconditioned responses of the animal. How-
ever, from the tests involving the change in sensitivity, we know that a magnet-
ic field affects unconditioned responses, changing the sensitivity of the animal
to unconditioned stimuli. It is fully probably that a magnetic field increases
the sensitivity of the animal to external and internal unconditioned stimuli,
and thereby increases its motor activity. These explanations are, of course,
only working hypotheses whose reliability must be checked in experiments on the

then it is probable that there is
the internal mechanism. However,

211

mechanism of the change in motor activity during the influence of different ex-
ternal and internal factors.

We again showed the similarity in the effect of light and a magnetic field,
since both agents change motor activity uniquely. But, while light acted more
strongly than a magnetic field during development of conditioned reflexes and
measurement of sensitivity, the magnetic field acted more strongly than light
during recording of the motor activity of bullfinches. Illumination increased
the average number of movements from 3,000 to 5,400, but a magnetic field in-
creased it to 7,500. Upon comparing the effect of a magnetic field on fish and
pigeons when the conditioned-reflex method was used, we observed a greater sen-
sitivity to this stimulus in fish. During the recording of motor activity, the
magnetic field had a stronger effect on birds. An increase in motor activity
during the influence of a magnetic field has been noted not only in birds
[ETdarov, Kholodov, 1964], but also in mice [Barnothy, 1960] and guinea pigs
[Gorshenina, 1963]. During the influence of an SHF field of the meter range,
a 20-30% increase was observed in human motor activity during sleep [Goncharuk
and Pivovarov, 1964].

Conclusions

1. A constant magnetic field Increases the motor activity of fish and /248
birds.

2. In birds the effect of a CMF is manifested as both a general increase
In the number of movements and a change in the dynamics of motor activity dur-
ing the test.

3. The effect of a magnetic field appears most clearly during conditions
of constant Illumination.

212

CHAPTER 9. CHANGES IN THE HISTOLOGICAL PICTURE OF THE BRAIN
DURING THE INFLUENCE OF ELECTROMAGNETIC FIELDS

In recording the electrical brain reaction to different EMF, we noted the
appearance of slow high-amplitude oscillations of potentials (see Part I) . In
recent years, certain investigators have attributed the main role in the forma-
tion of slow components of the EEC to glial elements [Galambos, 1961, 1962;
Aladzhalova, 1962; Sokolov, 1962; and others]. Recording the electrical activity
in the region of the medulla oblongata (area postrema) , which basically contains
glial cells, revealed a rhythm of 3-6 Hz and superslow oscillations [Aladzhalova,
Kol^tsova, 1964]. Recording the electrical activity of separate brain neurons
during the influence of a CMF provided less information on the changes which oc-
curred than recording the EEC. This circumstance forces us to assume that not
only neurons participate in reactions to EMF. Finally, morphological investi-
gations of the brain following an animal's stay in different EMF, indicated a
glial reaction to these influences. We noted a revived proliferation reaction
of the microglia In the brain following exposure of the animals to an SHF field

2 2

of average (40 mw/cm ) and low (10-20 mw/cm ) intensities, which indicated the

stimulating effect of an SHF field. Swelling of separate nerve cells was ob-
served at the same time [Tolgskaya et al. , I960]. A productive reaction of glia
was frequently more explicit following a 5-minute exposure to an SHF field than
after a 15-minute exposure [Dolina, 1961], These changes in the glia are con-
sidered to be a nonspecific protective reaction of the central nervous system.
Morphologists long ago noted the reaction of glia following exposure to ioniz-
ing radiation while neurons remained normal [Mogil'nitskiy and Podlyaschuk,
1929; Shefer, 1936; and others]. At the 1st Radiological Conference, L. 0. Or-
beli expressed an opinion concerning basic damage to glial cells and vessels /249
during irradiation. Studying the morphological structure of the brain from ir-
radiated dogs, L. L. Vannikov (1956, 1964) concluded that the glial tissue (es-
pecially the astrocytes) and the vascular system connected with it suffers pri-
ma^ry damage during irradiation. Especially acute degeneration of astroglia was
observed in the brain stem and in the region of the hypothalamus. Irradiation
of the head by x-rays, 150-930 R/min caused necrosis of oligodendroglia which
was maximal after 6-24 hours. There was no quantitative difference in the ef-
fects between the cerebral cortex and the brain stem, but the changes were ex-
pressed to a lesser degree in the cerebellum [Brownson et al., 1963]. Electron
microscopic investigation of the brain of animals irradiated with a dose of
15,000 R showed that glial cells are more sensitive than neurons [Pitcock, 1962],
An opinion has been expressed that glia can function as a receptor with respect
to a neuron [Hild, 1962].

These data serve as the basis for investigating the glia-neuron relation-
ships in the brain of animals subjected to a CMF with a strength of 200-300 Oe.
These tests were conducted together with Professor M. M. Aleksandrovskaya.

The reaction of neuroglia and neurons was determined by morphological me-
thods: The astrocytes were stained by the Cajal or Snesarev methods, the oligo-
dendrocytes and microglia by the Aleksandrovskaya method, and the nerve cells

213

by the Nissl method. The examination was conducted dynamically after the ani-
mals had stayed 1, 10 or 60-70 hours in a CMF. During these prolonged exposures
the animal v^as in the GMF for 3-7 hours at a time. Rats were placed completely
between the poles of the electromagnet, but only the heads of cats and rabbits
were subjected to the influence of a CMF. The animals were killed immediately
after exposure. The experiments were conducted on 9 rabbits (killed by the air
embolism method) , 5 cats (killed with nembutal) and 4 white rats (killed by de-
capitation) . The control animals were killed simultaneously by the same methods.
The animals were in a CMF 1 hour (3 rabbits) , 10 hours (3 rabbits and 2 cats)
and 60-70 hours (3 rabbits, 3 cats and 4 rats). The preliminary treatment of
the experimental results based on an analysis of the histological picture of the
sensorimotor cortex shows the following.

In rabbits, one hour after the start of the CMF influence, we noted a sharp
productive reaction of the astroglia and oligodendroglia involving hyperplasia
and hypertrophy of the cell bodies and processes. The neurons remained intact.

In rabbits and cats, 10-12 hours after the start of the influence the reac-
tion of the glia remained productive, involving the presence of perivascular and
marginal glial fibrosis, with swelling of the dendritic oligodendroglia and
hypertrophy of the drainage glia. The neurons underwent reversible changes in /250
the form of swelling and hyperchromatosis.

In rabbits, cats and rats, 60-70 hours after the start of the CMF influence,
we observed productive-dystrophic damage to the neuroglia involving swelling of
the oligodendrocytes and the appearance of drainage cells. Dystrophic damage
encompassed even the nerve cells. A picture of hypoxic encephalopathy with dys-
trophic changes of the glia was morphologically diagnosed (Figure 87).

This proffered material
allows us to assume that pri-
marily the neuroglia of the
brain react to the influence
of a CMF [Kholodov and Luk'-
yanova, 1964]. A. I. Ryzhov
(1964) noted a reaction of
the peripheral glia to a CMF.

Since the glial forma-
tions are distinguished by a
high metabolism, we can con-
sider that the effect of a
CMF on the brain is realized
to a significant degree
through changes in the meta-
bolism of neuroglia.

The previously preval-
Figure 87. The Change in the Histological Pic- ent opinion that glia perform
ture of the Rabbit Brain Following a 10-Hour only a support function has
Stay in a CMF. The Productive Reaction of As- been changed under the
trocytes in the Sensorimotor Cortex (Magnifi-
cation, 160).
214

Influence of new facts. Three types of glia are distlnugished: astroglia, cells
with many processes and a large nucleus; oligodendroglia, cells with a small nu-
cleus and few processes; and microglia. The first two types of glia are some-
times combined with the microglia. The closeness of glia to vessels forces us /251
to assume that glia are intermediaries between nerve and circulatory elements.
The high metabolism of glia keeps the activity of neurons at a sufficient level.
Galambos (1961) states that glial and neuronal elements are an entity and that
it is impossible to separate them.

Rhythmic pulsation of glial cells in a tissue culture has been observed un-
der electrical stimulation [Lumsden and Pomerat, 1951]. The glial cells con-
tracted with a latent period of 1.5-4.0 min. The duration of the contraction
varied from 1.4 to 3.4 min [Chang and Hild, 1959]. There are approximately 10
times more glial cells in the brain than neurons [Galambos, 1961; Blinkov and
Glezer, 1964]. It is widely accepted that glia play an important role in vari-
ous processes occurring in the brain in normal and pathological states, espec-
ially in the reaction of the brain to various damages, intoxications, infections,
traumas, and metabolic disturbances [Aleksandrovskaya, 1950; Snesarev, 1959;
Blinkov and Glezer, 1964]. Glia are distributed comparatively uniformly through-
out the gray and white matter of the brain, although there are data concerning a
significant predominance of glial cells in the rat hypothalamus in comparison
with other brain sections [Nurnberger, 1958].

Studying the experiments on the effect of EMF on the CNS, we can explain
many of the results by the effect of a CMF on the glia. The last two series of
experiments, involving the recording of neuronal activity and a morphological
analysis of the brain of rabbits subjected to a CMF, directly implicate the glia
in reactions to a CMF. In all our experiments we noted a prolonged latent peri-
od, measured in seconds and tens of seconds. The long latent period indicates
that neurons are not the primary elements reacting to the CMF since the latent
period of their reactions is measured in milliseconds. A significant afteref-
fect also characterizes the reaction of glial elements.

By embedding electrodes in various sections of the brain, we showed that
the most intensive reaction to a CMF is observed in the hypothalamus and in the
cerebral cortex. Why do these particular sections of the brain react most
strongly to a CMF? It was observed that, in contrast to other sections of the
brain, in the hypothalamus and the cortex we recorded superslow oscillations of
potential with variable parameters acting directly on the metabolism of the
"neuroglia-neuron" system [ Aladzhalova , 1962]. Consequently, the sensitivity
of these formation to a CMF can be explained by heightened metabolic processes
connected with the activity of glial elements.

Variations in the metabolic level, possibly reflected in superslow oscil- /252
lations of potential, may explain the statistical character of the reactions to
EMF.

The inhibiting effect of a CMF can also be explained via its influence on
glial elements. Actually, it has been shown that staphylococcus intoxication,
which causes a productive reaction of glia, is accompanied by the appearance of
defensive inhibition [Gorsheleva, 1957]. One can consider that activation of
the glia causes inhibition in the brain. The example also indicates the non-
specific character of glial reactions, which we observed using various EMF.

215

Conclusions

1. A one-hour exposure to a CMF causes a productive reaction of glia in
the animal's brain. In such a case, the neurons remain inactive.

2. An increase in the exposure duration of the CMF to 60-70 hours led to
productive-dystrophic damage of neuroglia. Dystrophic damage also encompassed
the nerve cells.

3. The described results lead to a conclusion concerning the effect of
EMF on neuron-glial relationships.

Synopsis

The experiments introduced in this section involving the prolonged exposure
of an organism to a CMF support and supplement the conclusions obtained during
the study of the physiological effect of EMF by the conditioned-reflex and
electrographic methods. Although we have studied only CMF, a comparison of the
results with the reference data (see Part I) indicates the similar effect of
UHF, SHF and constant magnetic fields. It is only necessary to recall the in-
hibition of signaling reflexes in frogs and the morphological picture of the
changes which occur in the brain following exposure to different EMF.

By determining the sensitivity to an electric current in fish, and to chem-
ical stimulation in frogs, we established that a magnetic field is a correcting
stimulus which is essentially inhibiting in character. The reactions to a mag-
netic field are effected with a long latent period (minutes) and are distin
guished by a prolonged aftereffect. A magnetic field and light have a similar
nonspecific effect on the sensitivity of blinded animals. The mechanism of the
reaction consists of the direct effect of these factors on the structures of
the diencephalon. All these conclusions coincide with the results of experi
ments given in the first two parts of this book.

The data concerning the increase in motor activity of animals during ex- /253
posure to a CMF are somewhat isolated. We also observed such a purely stimulat-
ing effect of EMF when we recorded the electrical brain activity (the appear-
ance of convulsive discharges) and when we developed conditioned reflexes (the
increase in the intersignal reactions) . The predominance of one or another
basic nervous process in the CNS is probably determined by the complex inter-
action of EMF with other weak and strong, external and internal stimuli that
continuously act on the CNS.

The morphological changes caused in the brain during exposure to EMF indi-
cate the paths for future analysis of this effect. It turned out that in the
glia-neuron complex, which occupies the greater part of brain tissue, the glia
undergo morphological changes sooner than the neurons. Since the neuron is
considered an electrically excitable structure, and the glia more sensitive to
chemical influences, we can assume that the primary effect of EMF is rendered
on certain chemical reactions of brain tissue.

216

GENERAL CONCLUSIONS /254

A discussion of questions on the mechanism of the effect of different EMF
on the organism should be Initiated with the essential physicochemical processes
occurring in a living cell during the influence of EMF. There can be several
such processes and we have not tried to enumerate them all. Let us simply re-
call that EMF can induce an electrical current, and that variable EMF of high
intensity can cause heating. Statements regarding the possibility of a reson-
ance effect of EMF on biological objects are encountered. The effect of EMF on
excitable structures can be connected with a change in the potassium-sodium
gradient in the cell due to oscillations of water molecules, hydrated ions, and
protein molecules in the surface layer of cell membranes [Presman, 1964b]. The
possibility of an effect of EMF on the structure of water was shown in the en-
gineering application of the so-called magnetic treatment of water, as a result
of which the precipitation of the dissolved salts decreased [Myagkov, 1960;
Lapotyshkina and Sazonov, 1961; and others]. EMF can polarize the side chains
of a protein molecule, causing cleavage of the hydrogen bonds and changing the
molecule hydration zone. There is a statement that strong CMF can affect the
orientation of macromolecules , in particular, MA and DNA molecules, and there-
by change biological processes [Dorfman, 1962]. The increase in the activity
of the enzjmies trypsin [Cook and Smith, 1964; Wiley et al., 1964] and carboxydis-
mutase [Akoyunoglou, 1964] under the effect of a CMF allows us to assume that
chemical changes play the main role in the primary mechanisms of the effect of
EMF.

The effect of EMF on excitable structures is similar to the effect of a dc
anode. It did not cause contractions of a neuromuscular preparation, but it
changed the chronaxy and reduced parabiosis. A UHF field [Vasil'yev and
Lapitskiy, 1938], an SHE field [Bychkov, 1962] and a CMF [Petrov, 1930; Erdman,
1956] acted in a similar manner.

At a definite intensity and exposure duration, the informational sensory
influence of an EMF on the organism predominates, and not its energy influence
[Barnothy, 1964]. This can be explained by the primary effect of EMF on the
functions of the CNS; in contrast to known neuronal impulsation, this effect i s/255
realized primarily through chemical processes in glial cells and is propagated
by some nonpulsed slow system [Rusinov, 1954; Aladzhalova, 1962; Becker et al.,
1962]. For example, in tests on lobster heart ganglia, it was shown that slow
changes in the membrane potential of one giant cell affect the discharge fre-
quency of the small cells located several millimeters away. This effect is
achieved without participation of nervous impulses [Watanabe and Bullock, I960].
We should note that the initial reactions of an organism to EMF are distinguished
by their nonspecificity. In expounding our material, we have frequently indi-
cated the nonspecificity of the effect of UHF, SHF and constant magnetic fields.
We should add that a high-voltage industrial frequency electrical field
[Sazopova, 1964], a low-frequency pulsed electrical field [Khvoles et al., 1962],
EMF of the sonic and radio-frequency ranges [Boyenko, 1963; Saley, 1964] and a
high-frequency field [Nikonova, 1964] have a similar effect on the CNS. Stimuli
of a nonelectromagnetic nature can also have a similar effect on electrical

217

brain activity and on developed conditioned reflexes. Here we must indicate
the nonspecific reaction of the CNS to a weak stimulus. It is possible that
protection from stimulation is no less important for an organism than percep-
tion of stimulation, and during the influence of a weak stimulus of any nature
we can observe reactions designated as preventative inhibition by certain
authors [Simonov, 1962],

Prolonged exposure to EMF not only involves the nervous system in the reac-
tion, but the hormonal, circulatory and other systems as well. For example,
during the influence of an SHF field, prolonged changes in the sympathetic-
adrenalin system have been observed [Yakovleva, 1964]. Consequently, the next
stage of the effect of EMF on an organism can be considered the development of
stress-type reactions [Sel'ye, 1960] which can also be observed during the mor-
phological disturbance of certain organs. We have usually been limited to a
study of the initial changes in the activity of the CNS. Since the majority of
experiments were conducted with a magnetic field, it is reasonable to introduce
certain results of the investigation of this factor.

Summary Table 49 provides a general characterization of the results of
tests on the effect of a magnetic field on different physiological processes of
vertebrates.

TABLE 49. THE EFFECT OF A MAGNETIC FIELD ON CER-
TAIN PHYSIOLOGICAL PROCESSES OF VERTEBRATES.

Character

of the
experiment

Fish

Amphibians

Birds

Mammals

CO
H

•H

>4-l

•

stabil-
ity

i-i
o

•

stabil-
ity

CO
•H

•

stabil-
ity

CO
14-1

•

stabil-
ity

0)
(0
CO
0)

u
o

0)
CO
CO
0)
M

0)
CO
cfl
0)

u
o

Q)
CO
cfl
0)

u
o

0)
CO

cS
0)

u
o
a

•rl

(U
en

u
o

0)
CO

u
o

0)
CO

CJ
0)

development of
a conditioned

reflex

—

none

influence on

conditioned

reflexes

—

influence on

sensitivity:

to an

electric

current

—

218

TABLE 49. (CONTINUED)

Character

of the
experiment

Fish

Amphibians

Birds

Mammals

<4-l

•

stabil-
ity

CO
Ci

cs
o

•

stabil-
ity

r-i
CO

.§

IH
O

•

stabil-
ity

"fi

•

stabil-
ity

<n
cd

u
u

CI
•H

O
(U
T3

0)
CO

u
o

CI
•H

cd
o

C
•H

0)
CO
CO
0)

M
O
C

CO
CO
(U
M
O
<U
X)

Influence on
sensitivity:

to acid
to light

Influence on
motor activity

influence on
the EEG

3
30

The dashes indicate that experiments of that type were not conducted. It
should be noted that the nvmiber of animals only approximately characterized the
volume of experimental work, since one animal was exposed many (up to hundreds)
times. The reaction stability is expressed in a percentage ratio of the number
of reactions to the number of exposures, and in the case of the influence of a
magnetic field on some activity, we have separately noted the stability of the
Increase and decrease in this activity.

Table 49 shows that in an overwhelming number of experiments, we observed /256
the effect of a magnetic field on physiological processes. Only the tests on
development of conditioned reflexes to a magnetic field in pigeons and rabbits
are exceptions. In spite of hundreds of combinations of the influence of a
field with food in pigeons and with electric stimulation in rabbits (the shak-
ing-off method) a conditioned response did not occur, which indicates the weak
nature of a magnetic field as a stimulus. Even if the conditioned reflex to a
magnetic field was developed (in fish) , its stability was 2 times less than the
stability of such reflexes to light or sound [Kholodov, 1958b]. The stability
of another reaction to a magnetic field (change in the EEG) was also low, being
equal to 53% [Kholodov, 1963a].

However, when the magnetic field acted on the background of some physiolog-
ical reaction, i.e., behaved as a correcting stimulus, then the reaction stabil-
ity was Increased approximately twice. For example, during development of a
conditioned reflex to a magnetic field, the reaction stability was 39%, but dur-
ing a study of the effect of a field on developed conditioned light and sound /257
reflexes the stability reached 88%. The time for the appearance of the

219

assimilation reaction on the rabbit EEG during the influence of interrupted
light of increasing brightness increased with statistical reliability (n < 0=05)
during exposure to the magnetic field. The stability of this reaction was 90%,
while changes on the spontaneous EEG during the influence of the field were ob-
served in only 53% of the cases. We managed to reveal only the correcting ef-
fect of a magnetic field in pigeons by the conditioned-reflex method. Thus, we
can conclude that a magnetic field is a weak correcting stimulus.

In what direction does a magnetic field change the current activity? In
tests on frogs, it was observed that on a background of increased sensitivity to
acid, a magnetic field reduced it, and on a background of reduced sensitivity,
it increased it. Thus, the results of the field influence depends on the ini-
tial functional state of the CNS. However, under ordinary experimental condi-
tions, we most frequently observed the inhibiting effect of the field. As is
evident from Table 49, the conditioned reflexes and the sensitivity to different
stimuli are reduced more frequently than increased. Only in the tests when we
recorded the motor activity did we see a predominance of excitation during the
influence of the field. However, in the development of conditioned reflexes to
a magnetic field in fish, inhibiting reflexes (conditioned inhibition) were de-
veloped much better than positive reflexes, and in recording the electrical ac-
tivity of the cerebral cortex during the influence of the field, we observed an
increase in the number of spindles and slow waves, which also appear when the
animal sleeps.

In different types of experiments, we observed an aftereffect immediately
after the electromagnet was turned off. This was manifested as sequential in-
hibition during development of conditioned inhibition to a magnetic field, as a
prolonged reduction in sensitivity (for several minutes) after the influence
ceased, and also as the EEG reaction to turn-off.

In determining the threshold of perception of a magnetic field, we observed
that in tests involving the recording of the rabbit EEG, it was approximately
100 Oe, in tests on development of conditioned reflexes in fish, it was 10-30
Oe, and in tests involving the recording of the motor activity of bullfinches,
it was 1-2 Oe. It is possible that under natural conditions, the changes in
the earth's magnetic field strength are threshold changes, and that what we ob-
tained in our experiments can serve as a more or less successful model of natu-
ral processes.

In connection with this, let us mention the experiments of Tromp (1939),
who showed that for certain people who can determine the presence of underground
water by the inhibition of the motor reaction of holding a willow stick at arm s
length, the threshold of perception of a CMF is below 0.001 Oe. Similar results
were recently obtained by Rocard (1964). Thus, a magnetic field is a weak cor-
recting stimulus, the reaction to which is effected following a significant JJ^
latent period and has a prolonged aftereffect. The threshold intensity is close
to the strength of the earth's magnetic field.

In the electrophysiological experiments, we observed similar elements in
the physiological effect of a UHF, an SHF and a constant magnetic field and io
nizing radiation. In tests involving different methods, we observed a similar-
ity in the effects of a CMF, light, a UHF field and ionizing radiation. If we

220

include the reference data concerning the characteristics of the development of
conditioned reflexes to a temperature stimulus, then the whole range of electro-
magnetic waves enters the sphere of our attention. Thus, different sections of
the spectrum of electromagnetic oscillations can have a similar physiological
effect on the CNS of vertebrates. This similarity is revealed more clearly when
specialized receptors for the perception of certain sections of this spectrum
are absent. As an example, the similarity in the effects of light and a CMF on
blinded fish is revealed more clearly than in intact fish.

The direct effect of EMF on the CNS serves as the physiological basis for
this similarity. A greater response to EMF is inherent to the cerebral cortex
and the diencephalon. In animals in which the cortex has not developed (fish) ,
the diencephalon (especially the hypothalamus) is the most reactive structure.

The greatest effect of EMF on the main regulatory center of the brain,
where the nervous and humoral paths of integration converge, forces us to as-
sume that even weak influences on this center can cause significant physiologi-
cal changes.

The insufficient attention devoted by neurophysiologists to the direct ef-
fect of stimuli on the CNS can be explained by many factors. The basic factor
was the absence of methods for directly determining the functional state of the
CNS. Experimental proofs of the direct effect of certain stimuli on the CNS
were obtained only after the wide introduction of electrophysiological methods
[Granit, 1957].

The predominance of morphological works for determining the relative par-
ticipation of the CNS in the reactions of the organism to penetrating factors
has led certain foreign investigators to an opinion concerning the high stabil-
ity of the CNS in comparison with other systems of the organism.

The introduction of different concepts regarding sensitivity and stability
[Livanov, 1962] can probably reconcile the opinions of morphologists and physi-
ologists concerning the position of the CNS in the reactions of an organism to
penetrating factors. The CNS remains extremely sensitive in a physiological
sense and stable in a morphological sense. Therefore, the CNS can react first /259
to a stimulus for which there is no specialized receptor and, perhaps, to a
stimulus for which a receptor exists. Then, the effect of any stimulus can be
examined as a polyreceptor effect. For example, in fish, the retina, the skin,
and the diencephalon can react to light. An increase in the intensity of a UHF
field can, apart from the CNS, lead to the involvement of thermal receptors,
pain receptors, and even muscle tissue in the reaction.

The subsensory character of the effect of penetrating factors is one of the
preventing reasons for not ascribing the properties of a physiological stimulus
to these factors. In this respect, the reception of EMF resembles the activity
of interoceptors. The slowness of the reaction to subsensory stimuli does not
allow us to attribute a signaling importance to them. It is on the basis of
this latter phenomenon that the fast motor reactions of an animal are formed.
Only in fish did we manage to develop conditioned reflexes to a magnetic field,
but in their stability these reactions significantly yielded to light and sound
conditioned reflexes. It is possible that EMF are the signals that determine
many rhythmic processes of an organism.

Two centuries have not yet passed since the time when the tests of Galvani
began electrophysiology. Now, electrophysiology is an important ^ essential
physiological science, but its development continues. The study of the role of
electromagnetic fields in the processes of vital activity must be considered one
of the most promising directions in this development. This should include both
investigations of the effect of electromagnetic fields on the functions of ani-
mals and plants and the clarification of the roles of EMF that occur during dif-
ferent physiological processes. This book is devoted to this first direction
of these Investigations. The investigation of the magnetic field created in a
nerve when a nervous pulse passes through [Seipel and Morrow, 1960] and the re-
cording of high-frequency electromagnetic fields during contraction of the hum-
an skeletal muscles [Volkers and Candib, 1960] can be related to the latter di-
rection.

The study of the biological role of EMF involves an examination of certain
general positions of physiological science, especially of neurophysiology. We
can already ask questions on the existence of slow regulatory systems, the pre-
sence of a receptor function in the brain, and about the polyafferent effect of
stimuli. This aspect of electrophysiology can provide an important contribution
to the development of subsensory stimulation and orientation of animals during
long-range migrations.

The problems of the biological effect of EMF, which was stated at the be-
ginning of this century by V. J. Danilewsky, can now be solved thanks to the
successes of electronics and computer technology, and also thanks to contempor-
ary methods of biological experimentation.

222

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FRANK C. FARNHAM COMPANY
133 South 36th Street
Philadelphia, Penna. 19104
Translated by: Frank Dion
NASw-1497

250 Ii'_l).65 NASA-Langley, 1967

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